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Q9C9P4

- KASC2_ARATH

UniProt

Q9C9P4 - KASC2_ARATH

Protein

3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic

Gene

KAS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Essential protein that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-16 and C-16 to unsaturated C-18 fatty acids. Confers resistance to low temperatures by maintaining chloroplast membranes integrity. Involved in the regulation of fatty acids ratios during seed metabolism. Required for embryo development, especially at the transition from the globular to the heart stage.6 Publications

    Catalytic activityi

    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei292 – 2921PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: TAIR

    GO - Biological processi

    1. cold acclimation Source: UniProtKB
    2. embryo development ending in seed dormancy Source: UniProtKB
    3. fatty acid biosynthetic process Source: TAIR
    4. unsaturated fatty acid biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Acyltransferase, Developmental protein, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT1G74960-MONOMER.
    ARA:GQT-1583-MONOMER.
    ARA:GQT-1593-MONOMER.
    ARA:MONOMER-14118.
    MetaCyc:MONOMER-14118.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic (EC:2.3.1.41)
    Alternative name(s):
    Beta-ketoacyl-acyl-carrier-protein synthase II
    Short name:
    AtKAS2
    Short name:
    Beta-ketoacyl-ACP synthetase 2
    Protein FATTY ACID BIOSYNTHESIS 1
    Gene namesi
    Name:KAS2
    Synonyms:FAB1
    Ordered Locus Names:At1g74960
    ORF Names:F25A4.7, F9E10.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G74960.

    Subcellular locationi

    Plastidchloroplast stroma 2 Publications

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Lethal when homozygous due to embryo abortion before the torpedo stage. Converts temperate oilseed composition (rich in unsaturated 18-carbon fatty acids) to that of a palm-like tropical oil (enriched in saturated 16-carbon fatty acids).2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi337 – 3371L → F in fab1; partial activity deficience due to structural instability and reduced substrate binding affinity, resulting in increased levels of saturated 16:0 but reduced levels of 18:0 fatty acids, particularly in chloroplasts, and associated with damage and death at continuous low temperature (accompanied by chloroplast degenerescence), but not after transient chilling or freezing. 5 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 103103ChloroplastSequence AnalysisAdd
    BLAST
    Chaini104 – 5414383-oxoacyl-[acyl-carrier-protein] synthase II, chloroplasticPRO_0000406094Add
    BLAST

    Proteomic databases

    PaxDbiQ9C9P4.
    PRIDEiQ9C9P4.

    Expressioni

    Tissue specificityi

    Mostly expressed in siliques, and, to a lower extent, in leaves, stems, flower buds, and flowers.1 Publication

    Developmental stagei

    First observed during the transition from the late globular to the early heart embryo stages. Later observed during heart, tropedo, and cotyledonary embryo stages. In seedlings, observed in the shoot apex and stomatal guard cells. In adult plants, expressed in inflorescences. In flowers, strongly present in styles and pollen grains.1 Publication

    Gene expression databases

    ArrayExpressiQ9C9P4.
    GenevestigatoriQ9C9P4.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi29054. 1 interaction.
    STRINGi3702.AT1G74960.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C9P4.
    SMRiQ9C9P4. Positions 130-537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the beta-ketoacyl-ACP synthases family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0304.
    HOGENOMiHOG000060166.
    InParanoidiQ9C9P4.
    KOiK09458.
    OMAiCMANALR.
    PhylomeDBiQ9C9P4.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 2 hits.
    TIGRFAMsiTIGR03150. fabF. 1 hit.
    PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9C9P4-1 [UniParc]FASTAAdd to Basket

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    MVGASSSYAS PLCTWFVAAC MSVSHGGGDS RQAVALQSGG RSRRRRQLSK    50
    CSVASGSASI QALVTSCLDF GPCTHYNNNN ALSSLFGSNS VSLNRNQRRL 100
    NRAASSGGAM AVMEMEKEAA VNKKPPTEQR RVVVTGMGVE TSLGHDPHTF 150
    YENLLQGNSG ISQIENFDCS EFPTRIAGEI KSFSTEGWVA PKLSKRMDKF 200
    MLYLLTAGKK ALADGGVTDE VMAEFDKTKC GVLIGSAMGG MKVFYDAIEA 250
    LRISYKKMNP FCVPFATTNM GSAMLAMDLG WMGPNYSIST ACATSNFCIL 300
    NSANHIIKGE ADVMLCGGSD AVIIPIGLGG FVACRALSQR NNDPTKASRP 350
    WDTNRDGFVM GEGAGVLLLE ELEHAKKRGA TIYAEFLGGS FTCDAYHMTE 400
    PHPDGAGVIL CIERALASAG ISKEQINYIN AHATSTHAGD IKEYQALAHC 450
    FGQNPELKVN STKSMIGHLL GAAGAVEAVA TVQAIRTGWV HPNINLENPD 500
    SGVDTKLLVG PKKERLDIKA ALSNSFGFGG HNSSIIFAPY K 541
    Length:541
    Mass (Da):57,600
    Last modified:June 1, 2001 - v1
    Checksum:iCB4F87E7B0C82189
    GO

    Sequence cautioni

    The sequence AAL06498.1 differs from that shown. Reason: Frameshift at positions 38 and 328.
    The sequence AAL06498.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAD55280.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti472 – 4721A → V in AAL91174. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF318307 mRNA. Translation: AAK69603.1.
    AC008263 Genomic DNA. Translation: AAD55280.1. Sequence problems.
    AC013258 Genomic DNA. Translation: AAG51920.1.
    CP002684 Genomic DNA. Translation: AEE35655.1.
    CP002684 Genomic DNA. Translation: AEE35656.1.
    CP002684 Genomic DNA. Translation: AEE35657.1.
    AF412045 mRNA. Translation: AAL06498.1. Frameshift.
    AF419598 mRNA. Translation: AAL31930.1.
    AY054196 mRNA. Translation: AAL06857.1.
    AY081285 mRNA. Translation: AAL91174.1.
    AY097344 mRNA. Translation: AAM19860.1.
    PIRiD96779.
    RefSeqiNP_001185400.1. NM_001198471.1.
    NP_565097.1. NM_106154.3.
    NP_849888.1. NM_179557.3.
    UniGeneiAt.26097.

    Genome annotation databases

    EnsemblPlantsiAT1G74960.1; AT1G74960.1; AT1G74960.
    AT1G74960.2; AT1G74960.2; AT1G74960.
    AT1G74960.3; AT1G74960.3; AT1G74960.
    GeneIDi843835.
    KEGGiath:AT1G74960.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF318307 mRNA. Translation: AAK69603.1 .
    AC008263 Genomic DNA. Translation: AAD55280.1 . Sequence problems.
    AC013258 Genomic DNA. Translation: AAG51920.1 .
    CP002684 Genomic DNA. Translation: AEE35655.1 .
    CP002684 Genomic DNA. Translation: AEE35656.1 .
    CP002684 Genomic DNA. Translation: AEE35657.1 .
    AF412045 mRNA. Translation: AAL06498.1 . Frameshift.
    AF419598 mRNA. Translation: AAL31930.1 .
    AY054196 mRNA. Translation: AAL06857.1 .
    AY081285 mRNA. Translation: AAL91174.1 .
    AY097344 mRNA. Translation: AAM19860.1 .
    PIRi D96779.
    RefSeqi NP_001185400.1. NM_001198471.1.
    NP_565097.1. NM_106154.3.
    NP_849888.1. NM_179557.3.
    UniGenei At.26097.

    3D structure databases

    ProteinModelPortali Q9C9P4.
    SMRi Q9C9P4. Positions 130-537.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29054. 1 interaction.
    STRINGi 3702.AT1G74960.1-P.

    Proteomic databases

    PaxDbi Q9C9P4.
    PRIDEi Q9C9P4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G74960.1 ; AT1G74960.1 ; AT1G74960 .
    AT1G74960.2 ; AT1G74960.2 ; AT1G74960 .
    AT1G74960.3 ; AT1G74960.3 ; AT1G74960 .
    GeneIDi 843835.
    KEGGi ath:AT1G74960.

    Organism-specific databases

    TAIRi AT1G74960.

    Phylogenomic databases

    eggNOGi COG0304.
    HOGENOMi HOG000060166.
    InParanoidi Q9C9P4.
    KOi K09458.
    OMAi CMANALR.
    PhylomeDBi Q9C9P4.

    Enzyme and pathway databases

    BioCyci ARA:AT1G74960-MONOMER.
    ARA:GQT-1583-MONOMER.
    ARA:GQT-1593-MONOMER.
    ARA:MONOMER-14118.
    MetaCyc:MONOMER-14118.

    Gene expression databases

    ArrayExpressi Q9C9P4.
    Genevestigatori Q9C9P4.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR017568. 3-oxoacyl-ACP_synth-2.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 2 hits.
    TIGRFAMsi TIGR03150. fabF. 1 hit.
    PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A KAS2 cDNA complements the phenotypes of the Arabidopsis fab1 mutant that differs in a single residue bordering the substrate binding pocket."
      Carlsson A.S., LaBrie S.T., Kinney A.J., von Wettstein-Knowles P., Browse J.
      Plant J. 29:761-770(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LEU-337.
      Strain: cv. Wassilewskija.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "A Mutant of Arabidopsis Deficient in the Elongation of Palmitic Acid."
      Wu J., James D.W. Jr., Dooner H.K., Browse J.
      Plant Physiol. 106:143-150(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF LEU-337.
      Strain: cv. Columbia.
    6. "Elevated levels of high-melting-point phosphatidylglycerols do not induce chilling sensitivity in an Arabidopsis mutant."
      Wu J., Browse J.
      Plant Cell 7:17-27(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LEU-337.
      Strain: cv. Columbia.
    7. "Low-temperature damage and subsequent recovery of fab1 mutant Arabidopsis exposed to 2 degrees C."
      Wu J., Lightner J., Warwick N., Browse J.
      Plant Physiol. 113:347-356(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LEU-337.
      Strain: cv. Columbia.
    8. "Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil."
      Pidkowich M.S., Nguyen H.T., Heilmann I., Ischebeck T., Shanklin J.
      Proc. Natl. Acad. Sci. U.S.A. 104:4742-4747(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LEU-337.
    9. "Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana."
      Hakozaki H., Park J.-I., Endo M., Takada Y., Kazama T., Takeda Y., Suzuki G., Kawagishi-Kobayashi M., Watanabe M.
      Genes Genet. Syst. 83:143-152(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: cv. Columbia.
    10. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKASC2_ARATH
    AccessioniPrimary (citable) accession number: Q9C9P4
    Secondary accession number(s): Q8RXF5, Q945N5, Q9SSG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3