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Q9C9P4 (KASC2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic

EC=2.3.1.41
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase II
Short name=AtKAS2
Short name=Beta-ketoacyl-ACP synthetase 2
Protein FATTY ACID BIOSYNTHESIS 1
Gene names
Name:KAS2
Synonyms:FAB1
Ordered Locus Names:At1g74960
ORF Names:F25A4.7, F9E10.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential protein that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-16 and C-16 to unsaturated C-18 fatty acids. Confers resistance to low temperatures by maintaining chloroplast membranes integrity. Involved in the regulation of fatty acids ratios during seed metabolism. Required for embryo development, especially at the transition from the globular to the heart stage. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Catalytic activity

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. Ref.5

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast stroma Ref.10 Ref.11.

Tissue specificity

Mostly expressed in siliques, and, to a lower extent, in leaves, stems, flower buds, and flowers. Ref.9

Developmental stage

First observed during the transition from the late globular to the early heart embryo stages. Later observed during heart, tropedo, and cotyledonary embryo stages. In seedlings, observed in the shoot apex and stomatal guard cells. In adult plants, expressed in inflorescences. In flowers, strongly present in styles and pollen grains. Ref.9

Disruption phenotype

Lethal when homozygous due to embryo abortion before the torpedo stage. Converts temperate oilseed composition (rich in unsaturated 18-carbon fatty acids) to that of a palm-like tropical oil (enriched in saturated 16-carbon fatty acids). Ref.8 Ref.9

Sequence similarities

Belongs to the beta-ketoacyl-ACP synthases family.

Sequence caution

The sequence AAD55280.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAL06498.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAL06498.1 differs from that shown. Reason: Frameshift at positions 38 and 328.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 103103Chloroplast Potential
Chain104 – 5414383-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic
PRO_0000406094

Sites

Active site2921 By similarity

Experimental info

Mutagenesis3371L → F in fab1; partial activity deficience due to structural instability and reduced substrate binding affinity, resulting in increased levels of saturated 16:0 but reduced levels of 18:0 fatty acids, particularly in chloroplasts, and associated with damage and death at continuous low temperature (accompanied by chloroplast degenerescence), but not after transient chilling or freezing. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8
Sequence conflict4721A → V in AAL91174. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9C9P4 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: CB4F87E7B0C82189

FASTA54157,600
        10         20         30         40         50         60 
MVGASSSYAS PLCTWFVAAC MSVSHGGGDS RQAVALQSGG RSRRRRQLSK CSVASGSASI 

        70         80         90        100        110        120 
QALVTSCLDF GPCTHYNNNN ALSSLFGSNS VSLNRNQRRL NRAASSGGAM AVMEMEKEAA 

       130        140        150        160        170        180 
VNKKPPTEQR RVVVTGMGVE TSLGHDPHTF YENLLQGNSG ISQIENFDCS EFPTRIAGEI 

       190        200        210        220        230        240 
KSFSTEGWVA PKLSKRMDKF MLYLLTAGKK ALADGGVTDE VMAEFDKTKC GVLIGSAMGG 

       250        260        270        280        290        300 
MKVFYDAIEA LRISYKKMNP FCVPFATTNM GSAMLAMDLG WMGPNYSIST ACATSNFCIL 

       310        320        330        340        350        360 
NSANHIIKGE ADVMLCGGSD AVIIPIGLGG FVACRALSQR NNDPTKASRP WDTNRDGFVM 

       370        380        390        400        410        420 
GEGAGVLLLE ELEHAKKRGA TIYAEFLGGS FTCDAYHMTE PHPDGAGVIL CIERALASAG 

       430        440        450        460        470        480 
ISKEQINYIN AHATSTHAGD IKEYQALAHC FGQNPELKVN STKSMIGHLL GAAGAVEAVA 

       490        500        510        520        530        540 
TVQAIRTGWV HPNINLENPD SGVDTKLLVG PKKERLDIKA ALSNSFGFGG HNSSIIFAPY 


K 

« Hide

References

« Hide 'large scale' references
[1]"A KAS2 cDNA complements the phenotypes of the Arabidopsis fab1 mutant that differs in a single residue bordering the substrate binding pocket."
Carlsson A.S., LaBrie S.T., Kinney A.J., von Wettstein-Knowles P., Browse J.
Plant J. 29:761-770(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LEU-337.
Strain: cv. Wassilewskija.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"A Mutant of Arabidopsis Deficient in the Elongation of Palmitic Acid."
Wu J., James D.W. Jr., Dooner H.K., Browse J.
Plant Physiol. 106:143-150(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF LEU-337.
Strain: cv. Columbia.
[6]"Elevated levels of high-melting-point phosphatidylglycerols do not induce chilling sensitivity in an Arabidopsis mutant."
Wu J., Browse J.
Plant Cell 7:17-27(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LEU-337.
Strain: cv. Columbia.
[7]"Low-temperature damage and subsequent recovery of fab1 mutant Arabidopsis exposed to 2 degrees C."
Wu J., Lightner J., Warwick N., Browse J.
Plant Physiol. 113:347-356(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LEU-337.
Strain: cv. Columbia.
[8]"Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil."
Pidkowich M.S., Nguyen H.T., Heilmann I., Ischebeck T., Shanklin J.
Proc. Natl. Acad. Sci. U.S.A. 104:4742-4747(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LEU-337.
[9]"Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana."
Hakozaki H., Park J.-I., Endo M., Takada Y., Kazama T., Takeda Y., Suzuki G., Kawagishi-Kobayashi M., Watanabe M.
Genes Genet. Syst. 83:143-152(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: cv. Columbia.
[10]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF318307 mRNA. Translation: AAK69603.1.
AC008263 Genomic DNA. Translation: AAD55280.1. Sequence problems.
AC013258 Genomic DNA. Translation: AAG51920.1.
CP002684 Genomic DNA. Translation: AEE35655.1.
CP002684 Genomic DNA. Translation: AEE35656.1.
CP002684 Genomic DNA. Translation: AEE35657.1.
AF412045 mRNA. Translation: AAL06498.1. Frameshift.
AF419598 mRNA. Translation: AAL31930.1.
AY054196 mRNA. Translation: AAL06857.1.
AY081285 mRNA. Translation: AAL91174.1.
AY097344 mRNA. Translation: AAM19860.1.
PIRD96779.
RefSeqNP_001185400.1. NM_001198471.1.
NP_565097.1. NM_106154.3.
NP_849888.1. NM_179557.3.
UniGeneAt.26097.

3D structure databases

ProteinModelPortalQ9C9P4.
SMRQ9C9P4. Positions 130-537.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid29054. 1 interaction.
STRING3702.AT1G74960.1-P.

Proteomic databases

PaxDbQ9C9P4.
PRIDEQ9C9P4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G74960.1; AT1G74960.1; AT1G74960.
AT1G74960.2; AT1G74960.2; AT1G74960.
AT1G74960.3; AT1G74960.3; AT1G74960.
GeneID843835.
KEGGath:AT1G74960.

Organism-specific databases

TAIRAT1G74960.

Phylogenomic databases

eggNOGCOG0304.
HOGENOMHOG000060166.
InParanoidQ9C9P4.
KOK09458.
OMAITPIGNT.
PhylomeDBQ9C9P4.
ProtClustDBPLN02787.

Enzyme and pathway databases

BioCycARA:AT1G74960-MONOMER.
ARA:GQT-1583-MONOMER.
ARA:GQT-1593-MONOMER.
ARA:MONOMER-14118.
MetaCyc:MONOMER-14118.

Gene expression databases

ArrayExpressQ9C9P4.
GenevestigatorQ9C9P4.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 2 hits.
TIGRFAMsTIGR03150. fabF. 1 hit.
PROSITEPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKASC2_ARATH
AccessionPrimary (citable) accession number: Q9C9P4
Secondary accession number(s): Q8RXF5, Q945N5, Q9SSG8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names