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Q9C9P4

- KASC2_ARATH

UniProt

Q9C9P4 - KASC2_ARATH

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Protein

3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic

Gene

KAS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential protein that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-16 and C-16 to unsaturated C-18 fatty acids. Confers resistance to low temperatures by maintaining chloroplast membranes integrity. Involved in the regulation of fatty acids ratios during seed metabolism. Required for embryo development, especially at the transition from the globular to the heart stage.6 Publications

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei292 – 2921PROSITE-ProRule annotation

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: TAIR

GO - Biological processi

  1. cold acclimation Source: UniProtKB
  2. embryo development ending in seed dormancy Source: UniProtKB
  3. fatty acid biosynthetic process Source: TAIR
  4. unsaturated fatty acid biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Developmental protein, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT1G74960-MONOMER.
ARA:GQT-1583-MONOMER.
ARA:GQT-1593-MONOMER.
ARA:MONOMER-14118.
MetaCyc:MONOMER-14118.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase II
Short name:
AtKAS2
Short name:
Beta-ketoacyl-ACP synthetase 2
Protein FATTY ACID BIOSYNTHESIS 1
Gene namesi
Name:KAS2
Synonyms:FAB1
Ordered Locus Names:At1g74960
ORF Names:F25A4.7, F9E10.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G74960.

Subcellular locationi

Plastidchloroplast stroma 2 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal when homozygous due to embryo abortion before the torpedo stage. Converts temperate oilseed composition (rich in unsaturated 18-carbon fatty acids) to that of a palm-like tropical oil (enriched in saturated 16-carbon fatty acids).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi337 – 3371L → F in fab1; partial activity deficience due to structural instability and reduced substrate binding affinity, resulting in increased levels of saturated 16:0 but reduced levels of 18:0 fatty acids, particularly in chloroplasts, and associated with damage and death at continuous low temperature (accompanied by chloroplast degenerescence), but not after transient chilling or freezing. 5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 103103ChloroplastSequence AnalysisAdd
BLAST
Chaini104 – 5414383-oxoacyl-[acyl-carrier-protein] synthase II, chloroplasticPRO_0000406094Add
BLAST

Proteomic databases

PaxDbiQ9C9P4.
PRIDEiQ9C9P4.

Expressioni

Tissue specificityi

Mostly expressed in siliques, and, to a lower extent, in leaves, stems, flower buds, and flowers.1 Publication

Developmental stagei

First observed during the transition from the late globular to the early heart embryo stages. Later observed during heart, tropedo, and cotyledonary embryo stages. In seedlings, observed in the shoot apex and stomatal guard cells. In adult plants, expressed in inflorescences. In flowers, strongly present in styles and pollen grains.1 Publication

Gene expression databases

ExpressionAtlasiQ9C9P4. baseline and differential.
GenevestigatoriQ9C9P4.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi29054. 1 interaction.
STRINGi3702.AT1G74960.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C9P4.
SMRiQ9C9P4. Positions 130-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0304.
HOGENOMiHOG000060166.
InParanoidiQ9C9P4.
KOiK09458.
OMAiCMANALR.
PhylomeDBiQ9C9P4.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C9P4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVGASSSYAS PLCTWFVAAC MSVSHGGGDS RQAVALQSGG RSRRRRQLSK
60 70 80 90 100
CSVASGSASI QALVTSCLDF GPCTHYNNNN ALSSLFGSNS VSLNRNQRRL
110 120 130 140 150
NRAASSGGAM AVMEMEKEAA VNKKPPTEQR RVVVTGMGVE TSLGHDPHTF
160 170 180 190 200
YENLLQGNSG ISQIENFDCS EFPTRIAGEI KSFSTEGWVA PKLSKRMDKF
210 220 230 240 250
MLYLLTAGKK ALADGGVTDE VMAEFDKTKC GVLIGSAMGG MKVFYDAIEA
260 270 280 290 300
LRISYKKMNP FCVPFATTNM GSAMLAMDLG WMGPNYSIST ACATSNFCIL
310 320 330 340 350
NSANHIIKGE ADVMLCGGSD AVIIPIGLGG FVACRALSQR NNDPTKASRP
360 370 380 390 400
WDTNRDGFVM GEGAGVLLLE ELEHAKKRGA TIYAEFLGGS FTCDAYHMTE
410 420 430 440 450
PHPDGAGVIL CIERALASAG ISKEQINYIN AHATSTHAGD IKEYQALAHC
460 470 480 490 500
FGQNPELKVN STKSMIGHLL GAAGAVEAVA TVQAIRTGWV HPNINLENPD
510 520 530 540
SGVDTKLLVG PKKERLDIKA ALSNSFGFGG HNSSIIFAPY K
Length:541
Mass (Da):57,600
Last modified:June 1, 2001 - v1
Checksum:iCB4F87E7B0C82189
GO

Sequence cautioni

The sequence AAD55280.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAL06498.1 differs from that shown. Reason: Frameshift at positions 38 and 328. Curated
The sequence AAL06498.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti472 – 4721A → V in AAL91174. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF318307 mRNA. Translation: AAK69603.1.
AC008263 Genomic DNA. Translation: AAD55280.1. Sequence problems.
AC013258 Genomic DNA. Translation: AAG51920.1.
CP002684 Genomic DNA. Translation: AEE35655.1.
CP002684 Genomic DNA. Translation: AEE35656.1.
CP002684 Genomic DNA. Translation: AEE35657.1.
AF412045 mRNA. Translation: AAL06498.1. Frameshift.
AF419598 mRNA. Translation: AAL31930.1.
AY054196 mRNA. Translation: AAL06857.1.
AY081285 mRNA. Translation: AAL91174.1.
AY097344 mRNA. Translation: AAM19860.1.
PIRiD96779.
RefSeqiNP_001185400.1. NM_001198471.1.
NP_565097.1. NM_106154.3.
NP_849888.1. NM_179557.3.
UniGeneiAt.26097.

Genome annotation databases

EnsemblPlantsiAT1G74960.1; AT1G74960.1; AT1G74960.
AT1G74960.2; AT1G74960.2; AT1G74960.
AT1G74960.3; AT1G74960.3; AT1G74960.
GeneIDi843835.
KEGGiath:AT1G74960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF318307 mRNA. Translation: AAK69603.1 .
AC008263 Genomic DNA. Translation: AAD55280.1 . Sequence problems.
AC013258 Genomic DNA. Translation: AAG51920.1 .
CP002684 Genomic DNA. Translation: AEE35655.1 .
CP002684 Genomic DNA. Translation: AEE35656.1 .
CP002684 Genomic DNA. Translation: AEE35657.1 .
AF412045 mRNA. Translation: AAL06498.1 . Frameshift.
AF419598 mRNA. Translation: AAL31930.1 .
AY054196 mRNA. Translation: AAL06857.1 .
AY081285 mRNA. Translation: AAL91174.1 .
AY097344 mRNA. Translation: AAM19860.1 .
PIRi D96779.
RefSeqi NP_001185400.1. NM_001198471.1.
NP_565097.1. NM_106154.3.
NP_849888.1. NM_179557.3.
UniGenei At.26097.

3D structure databases

ProteinModelPortali Q9C9P4.
SMRi Q9C9P4. Positions 130-537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 29054. 1 interaction.
STRINGi 3702.AT1G74960.1-P.

Proteomic databases

PaxDbi Q9C9P4.
PRIDEi Q9C9P4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G74960.1 ; AT1G74960.1 ; AT1G74960 .
AT1G74960.2 ; AT1G74960.2 ; AT1G74960 .
AT1G74960.3 ; AT1G74960.3 ; AT1G74960 .
GeneIDi 843835.
KEGGi ath:AT1G74960.

Organism-specific databases

TAIRi AT1G74960.

Phylogenomic databases

eggNOGi COG0304.
HOGENOMi HOG000060166.
InParanoidi Q9C9P4.
KOi K09458.
OMAi CMANALR.
PhylomeDBi Q9C9P4.

Enzyme and pathway databases

BioCyci ARA:AT1G74960-MONOMER.
ARA:GQT-1583-MONOMER.
ARA:GQT-1593-MONOMER.
ARA:MONOMER-14118.
MetaCyc:MONOMER-14118.

Gene expression databases

ExpressionAtlasi Q9C9P4. baseline and differential.
Genevestigatori Q9C9P4.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 2 hits.
TIGRFAMsi TIGR03150. fabF. 1 hit.
PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A KAS2 cDNA complements the phenotypes of the Arabidopsis fab1 mutant that differs in a single residue bordering the substrate binding pocket."
    Carlsson A.S., LaBrie S.T., Kinney A.J., von Wettstein-Knowles P., Browse J.
    Plant J. 29:761-770(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LEU-337.
    Strain: cv. Wassilewskija.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "A Mutant of Arabidopsis Deficient in the Elongation of Palmitic Acid."
    Wu J., James D.W. Jr., Dooner H.K., Browse J.
    Plant Physiol. 106:143-150(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF LEU-337.
    Strain: cv. Columbia.
  6. "Elevated levels of high-melting-point phosphatidylglycerols do not induce chilling sensitivity in an Arabidopsis mutant."
    Wu J., Browse J.
    Plant Cell 7:17-27(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LEU-337.
    Strain: cv. Columbia.
  7. "Low-temperature damage and subsequent recovery of fab1 mutant Arabidopsis exposed to 2 degrees C."
    Wu J., Lightner J., Warwick N., Browse J.
    Plant Physiol. 113:347-356(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LEU-337.
    Strain: cv. Columbia.
  8. "Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil."
    Pidkowich M.S., Nguyen H.T., Heilmann I., Ischebeck T., Shanklin J.
    Proc. Natl. Acad. Sci. U.S.A. 104:4742-4747(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LEU-337.
  9. "Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana."
    Hakozaki H., Park J.-I., Endo M., Takada Y., Kazama T., Takeda Y., Suzuki G., Kawagishi-Kobayashi M., Watanabe M.
    Genes Genet. Syst. 83:143-152(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  10. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKASC2_ARATH
AccessioniPrimary (citable) accession number: Q9C9P4
Secondary accession number(s): Q8RXF5, Q945N5, Q9SSG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3