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Protein

Cyclin-dependent kinase D-2

Gene

CDKD-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms a stable complex with cyclin CYCH1-1 that phosphorylates human CDK2 and the C-terminal domain (CTD) of the large subunit of RNA polymerase II.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Activated by phosphorylation by CDKF-1. Down-regulated by phosphorylation by WEE1.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATPCurated1
Active sitei135Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • positive regulation of long-day photoperiodism, flowering Source: TAIR
  • positive regulation of transcription by RNA polymerase II Source: GO_Central
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-113418 Formation of the Early Elongation Complex
R-ATH-5696395 Formation of Incision Complex in GG-NER
R-ATH-674695 RNA Polymerase II Pre-transcription Events
R-ATH-6781823 Formation of TC-NER Pre-Incision Complex
R-ATH-6782135 Dual incision in TC-NER
R-ATH-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-ATH-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-ATH-69202 Cyclin E associated events during G1/S transition
R-ATH-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-ATH-69656 Cyclin A:Cdk2-associated events at S phase entry
R-ATH-72086 mRNA Capping
R-ATH-73776 RNA Polymerase II Promoter Escape
R-ATH-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-ATH-75953 RNA Polymerase II Transcription Initiation
R-ATH-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-ATH-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase D-2 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKD;2
Alternative name(s):
CDK-activating kinase 4-At
Short name:
CAK4-At
Gene namesi
Name:CDKD-2
Ordered Locus Names:At1g66750
ORF Names:F4N21.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G66750
TAIRilocus:2033349 AT1G66750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24Y → F: Abolishes phosphorylation by WEE1. 1 Publication1
Mutagenesisi42K → R: Prevents autophosphorylation. 1 Publication1
Mutagenesisi162S → A: Reduces phosphorylation by CDKF-1 by 30%. 1 Publication1
Mutagenesisi168T → A: Almost abolishes phosphorylation by CDKF-1. Loss of CTD-kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931201 – 348Cyclin-dependent kinase D-2Add BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Phosphotyrosine1 Publication1
Modified residuei162Phosphoserine; by CAK1 Publication1
Modified residuei168Phosphothreonine; by CAK1 Publication1

Post-translational modificationi

Phosphorylated by CDKF-1 at Ser-162 and Thr-168. Phosphorylated by WEE1 at Tyr-24. Autophosphorylated.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C9M7
PRIDEiQ9C9M7

PTM databases

iPTMnetiQ9C9M7

Expressioni

Tissue specificityi

Expressed in suspension cell culture, but not in plant organs.1 Publication

Gene expression databases

ExpressionAtlasiQ9C9M7 baseline and differential
GenevisibleiQ9C9M7 AT

Interactioni

Subunit structurei

Interacts with CYCH1-1. Binding to CYCH1-1 activates CDK kinase.2 Publications

Protein-protein interaction databases

BioGridi28214, 43 interactors
IntActiQ9C9M7, 21 interactors
STRINGi3702.AT1G66750.1

Structurei

3D structure databases

ProteinModelPortaliQ9C9M7
SMRiQ9C9M7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST281

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0659 Eukaryota
ENOG410XQDH LUCA
HOGENOMiHOG000233024
InParanoidiQ9C9M7
KOiK02202
OMAiLAKMFIY
OrthoDBiEOG09360B04
PhylomeDBiQ9C9M7

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9C9M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSGDNQPV DRYLRRQILG EGTYGVVYKA TDTKTGKTVA VKKIRLGNQK
60 70 80 90 100
EGVNFTALRE IKLLKELNHP HIVELIDAFP HDGSLHLVFE YMQTDLEAVI
110 120 130 140 150
RDRNIFLSPG DIKSYMLMTL KGLAYCHKKW VLHRDMKPNN LLIGENGLLK
160 170 180 190 200
LADFGLARLF GSPNRRFTHQ VFATWYRAPE LLFGSRQYGA GVDVWAAGCI
210 220 230 240 250
FAELLLRRPF LPGSTEIDQL GKIFQAFGTP VPSQWSDMIY LPDYMEFSYT
260 270 280 290 300
PAPPLRTIFP MASDDALDLL AKMFIYDPRQ RITIQQALDH RYFSSSPSPT
310 320 330 340
EPGKLQIPAS KGDALEPKAS EQNQHGNSPA VLSPPGKMRR VMGPEGFT
Length:348
Mass (Da):39,201
Last modified:June 1, 2001 - v1
Checksum:iA32CA3E98204DDAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074116 mRNA Translation: BAB91558.1
AC013288 Genomic DNA Translation: AAG60076.1
CP002684 Genomic DNA Translation: AEE34551.1
AY136355 mRNA Translation: AAM97021.1
BT000198 mRNA Translation: AAN15517.1
RefSeqiNP_176847.1, NM_105345.5
UniGeneiAt.35737

Genome annotation databases

EnsemblPlantsiAT1G66750.1; AT1G66750.1; AT1G66750
GeneIDi842993
GrameneiAT1G66750.1; AT1G66750.1; AT1G66750
KEGGiath:AT1G66750

Similar proteinsi

Entry informationi

Entry nameiCDKD2_ARATH
AccessioniPrimary (citable) accession number: Q9C9M7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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