Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cyclin-dependent kinase D-2

Gene

CDKD-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a stable complex with cyclin CYCH1-1 that phosphorylates human CDK2 and the C-terminal domain (CTD) of the large subunit of RNA polymerase II.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Activated by phosphorylation by CDKF-1. Down-regulated by phosphorylation by WEE1.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATPCurated1
Active sitei135Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-113418. Formation of the Early Elongation Complex.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
R-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-ATH-69273. Cyclin A/B1 associated events during G2/M transition.
R-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-ATH-72086. mRNA Capping.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-ATH-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase D-2 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKD;2
Alternative name(s):
CDK-activating kinase 4-At
Short name:
CAK4-At
Gene namesi
Name:CDKD-2
Ordered Locus Names:At1g66750
ORF Names:F4N21.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2033349. AT1G66750.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
  • TFIIK complex Source: GO_Central

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24Y → F: Abolishes phosphorylation by WEE1. 1 Publication1
Mutagenesisi42K → R: Prevents autophosphorylation. 1 Publication1
Mutagenesisi162S → A: Reduces phosphorylation by CDKF-1 by 30%. 1 Publication1
Mutagenesisi168T → A: Almost abolishes phosphorylation by CDKF-1. Loss of CTD-kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931201 – 348Cyclin-dependent kinase D-2Add BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Phosphotyrosine1 Publication1
Modified residuei162Phosphoserine; by CAK1 Publication1
Modified residuei168Phosphothreonine; by CAK1 Publication1

Post-translational modificationi

Phosphorylated by CDKF-1 at Ser-162 and Thr-168. Phosphorylated by WEE1 at Tyr-24. Autophosphorylated.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C9M7.

PTM databases

iPTMnetiQ9C9M7.

Expressioni

Tissue specificityi

Expressed in suspension cell culture, but not in plant organs.1 Publication

Gene expression databases

ExpressionAtlasiQ9C9M7. baseline and differential.
GenevisibleiQ9C9M7. AT.

Interactioni

Subunit structurei

Interacts with CYCH1-1. Binding to CYCH1-1 activates CDK kinase.2 Publications

Protein-protein interaction databases

BioGridi28214. 43 interactors.
IntActiQ9C9M7. 21 interactors.
STRINGi3702.AT1G66750.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C9M7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST281

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0659. Eukaryota.
ENOG410XQDH. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9C9M7.
KOiK02202.
OMAiLAKMFTY.
OrthoDBiEOG09360B04.
PhylomeDBiQ9C9M7.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C9M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSGDNQPV DRYLRRQILG EGTYGVVYKA TDTKTGKTVA VKKIRLGNQK
60 70 80 90 100
EGVNFTALRE IKLLKELNHP HIVELIDAFP HDGSLHLVFE YMQTDLEAVI
110 120 130 140 150
RDRNIFLSPG DIKSYMLMTL KGLAYCHKKW VLHRDMKPNN LLIGENGLLK
160 170 180 190 200
LADFGLARLF GSPNRRFTHQ VFATWYRAPE LLFGSRQYGA GVDVWAAGCI
210 220 230 240 250
FAELLLRRPF LPGSTEIDQL GKIFQAFGTP VPSQWSDMIY LPDYMEFSYT
260 270 280 290 300
PAPPLRTIFP MASDDALDLL AKMFIYDPRQ RITIQQALDH RYFSSSPSPT
310 320 330 340
EPGKLQIPAS KGDALEPKAS EQNQHGNSPA VLSPPGKMRR VMGPEGFT
Length:348
Mass (Da):39,201
Last modified:June 1, 2001 - v1
Checksum:iA32CA3E98204DDAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074116 mRNA. Translation: BAB91558.1.
AC013288 Genomic DNA. Translation: AAG60076.1.
CP002684 Genomic DNA. Translation: AEE34551.1.
AY136355 mRNA. Translation: AAM97021.1.
BT000198 mRNA. Translation: AAN15517.1.
RefSeqiNP_176847.1. NM_105345.5.
UniGeneiAt.35737.

Genome annotation databases

EnsemblPlantsiAT1G66750.1; AT1G66750.1; AT1G66750.
GeneIDi842993.
GrameneiAT1G66750.1; AT1G66750.1; AT1G66750.
KEGGiath:AT1G66750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074116 mRNA. Translation: BAB91558.1.
AC013288 Genomic DNA. Translation: AAG60076.1.
CP002684 Genomic DNA. Translation: AEE34551.1.
AY136355 mRNA. Translation: AAM97021.1.
BT000198 mRNA. Translation: AAN15517.1.
RefSeqiNP_176847.1. NM_105345.5.
UniGeneiAt.35737.

3D structure databases

ProteinModelPortaliQ9C9M7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28214. 43 interactors.
IntActiQ9C9M7. 21 interactors.
STRINGi3702.AT1G66750.1.

PTM databases

iPTMnetiQ9C9M7.

Proteomic databases

PaxDbiQ9C9M7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66750.1; AT1G66750.1; AT1G66750.
GeneIDi842993.
GrameneiAT1G66750.1; AT1G66750.1; AT1G66750.
KEGGiath:AT1G66750.

Organism-specific databases

AraportiAT1G66750.
TAIRilocus:2033349. AT1G66750.

Phylogenomic databases

eggNOGiKOG0659. Eukaryota.
ENOG410XQDH. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9C9M7.
KOiK02202.
OMAiLAKMFTY.
OrthoDBiEOG09360B04.
PhylomeDBiQ9C9M7.

Enzyme and pathway databases

ReactomeiR-ATH-113418. Formation of the Early Elongation Complex.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
R-ATH-674695. RNA Polymerase II Pre-transcription Events.
R-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-ATH-69273. Cyclin A/B1 associated events during G2/M transition.
R-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-ATH-72086. mRNA Capping.
R-ATH-73776. RNA Polymerase II Promoter Escape.
R-ATH-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-ATH-75953. RNA Polymerase II Transcription Initiation.
R-ATH-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-ATH-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiQ9C9M7.

Gene expression databases

ExpressionAtlasiQ9C9M7. baseline and differential.
GenevisibleiQ9C9M7. AT.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCDKD2_ARATH
AccessioniPrimary (citable) accession number: Q9C9M7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2001
Last modified: February 15, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.