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Protein

Heme oxygenase 3, chloroplastic

Gene

HO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Plays a minor role in phytochrome assembly and photomorphogenesis.2 Publications

Catalytic activityi

Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • heme binding Source: TAIR
  • heme oxygenase (decyclizing) activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G69720-MONOMER.
MetaCyc:AT1G69720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heme oxygenase 3, chloroplastic (EC:1.14.99.3)
Gene namesi
Name:HO3
Ordered Locus Names:At1g69720
ORF Names:T6C23.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G69720.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5858ChloroplastSequence AnalysisAdd
BLAST
Chaini59 – 285227Heme oxygenase 3, chloroplasticPRO_0000412187Add
BLAST

Proteomic databases

PRIDEiQ9C9L4.

Expressioni

Tissue specificityi

Widely expressed at low levels.1 Publication

Gene expression databases

GenevestigatoriQ9C9L4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G69720.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C9L4.
SMRiQ9C9L4. Positions 153-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heme oxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG308332.
HOGENOMiHOG000265822.
InParanoidiQ9C9L4.
KOiK00510.
OMAiGKTYSEY.
PhylomeDBiQ9C9L4.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
InterProiIPR016053. Haem_Oase-like.
IPR016084. Haem_Oase-like_multi-hlx.
IPR016951. Haem_Oase_decyc_pln.
[Graphical view]
PfamiPF01126. Heme_oxygenase. 1 hit.
[Graphical view]
PIRSFiPIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAMiSSF48613. SSF48613. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C9L4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTRLNPSC HFPASTRLSC ESYLGLRTTG RISYARTLTA PRGYLAVKAN
60 70 80 90 100
GGQASVVTAA AITEKQQKKY PGESKGFVEE MRFVAMRLHT KDQAREGEKE
110 120 130 140 150
SRSPEEGPVA KWEPTVEGYL HFLVDSKLVY DTLEGIIDGS NFPTYAGFKN
160 170 180 190 200
TGLERAESLR KDLEWFKEQG YEIPEPMAPG KTYSEYLKDL AENDPQAFIC
210 220 230 240 250
HFYNIYFAHS AGGQMIGTKV SKKILDNKEL EFYKWDGQLS QLLQNVRQKL
260 270 280
NKVAEWWTRE EKSHCLEETE KSFKFSGEIL RLILS
Length:285
Mass (Da):32,434
Last modified:June 1, 2001 - v1
Checksum:iD552E638E7DFF241
GO
Isoform 2 (identifier: Q9C9L4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-227: VSKKILDN → LCRYLRRY
     228-285: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:227
Mass (Da):25,607
Checksum:iDC1F3852838F4265
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei220 – 2278VSKKILDN → LCRYLRRY in isoform 2. CuratedVSP_041653
Alternative sequencei228 – 28558Missing in isoform 2. CuratedVSP_041654Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320022 Genomic DNA. Translation: AAK63006.1.
AC013289 Genomic DNA. Translation: AAG52552.1.
CP002684 Genomic DNA. Translation: AEE34966.1.
CP002684 Genomic DNA. Translation: AEE34967.1.
AY084250 mRNA. Translation: AAM60844.1.
PIRiB96719.
RefSeqiNP_001117574.1. NM_001124102.1. [Q9C9L4-2]
NP_177130.1. NM_105640.3. [Q9C9L4-1]
UniGeneiAt.35382.
At.71252.

Genome annotation databases

EnsemblPlantsiAT1G69720.1; AT1G69720.1; AT1G69720. [Q9C9L4-1]
GeneIDi843308.
KEGGiath:AT1G69720.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320022 Genomic DNA. Translation: AAK63006.1.
AC013289 Genomic DNA. Translation: AAG52552.1.
CP002684 Genomic DNA. Translation: AEE34966.1.
CP002684 Genomic DNA. Translation: AEE34967.1.
AY084250 mRNA. Translation: AAM60844.1.
PIRiB96719.
RefSeqiNP_001117574.1. NM_001124102.1. [Q9C9L4-2]
NP_177130.1. NM_105640.3. [Q9C9L4-1]
UniGeneiAt.35382.
At.71252.

3D structure databases

ProteinModelPortaliQ9C9L4.
SMRiQ9C9L4. Positions 153-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G69720.1-P.

Proteomic databases

PRIDEiQ9C9L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G69720.1; AT1G69720.1; AT1G69720. [Q9C9L4-1]
GeneIDi843308.
KEGGiath:AT1G69720.

Organism-specific databases

TAIRiAT1G69720.

Phylogenomic databases

eggNOGiNOG308332.
HOGENOMiHOG000265822.
InParanoidiQ9C9L4.
KOiK00510.
OMAiGKTYSEY.
PhylomeDBiQ9C9L4.

Enzyme and pathway databases

BioCyciARA:AT1G69720-MONOMER.
MetaCyc:AT1G69720-MONOMER.

Miscellaneous databases

PROiQ9C9L4.

Gene expression databases

GenevestigatoriQ9C9L4.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
InterProiIPR016053. Haem_Oase-like.
IPR016084. Haem_Oase-like_multi-hlx.
IPR016951. Haem_Oase_decyc_pln.
[Graphical view]
PfamiPF01126. Heme_oxygenase. 1 hit.
[Graphical view]
PIRSFiPIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAMiSSF48613. SSF48613. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants."
    Davis S.J., Bhoo S.H., Durski A.M., Walker J.M., Vierstra R.D.
    Plant Physiol. 126:656-669(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis."
    Emborg T.J., Walker J.M., Noh B., Vierstra R.D.
    Plant Physiol. 140:856-868(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. "Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions."
    Gisk B., Yasui Y., Kohchi T., Frankenberg-Dinkel N.
    Biochem. J. 425:425-434(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiHMOX3_ARATH
AccessioniPrimary (citable) accession number: Q9C9L4
Secondary accession number(s): B3H531
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: June 1, 2001
Last modified: April 29, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.