Q9C9L4 (HMOX3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme oxygenase 3, chloroplastic
EC=1.14.99.3

Gene names

Name:	HO3
Ordered Locus Names:	At1g69720
ORF Names:	T6C23.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Plays a minor role in phytochrome assembly and photomorphogenesis. Ref.5 Ref.6
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.
Subcellular location	Plastid › chloroplast Ref.6.
Tissue specificity	Widely expressed at low levels. Ref.5
Disruption phenotype	No visible phenotype under normal growth conditions. Ref.5
Sequence similarities	Belongs to the heme oxygenase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Ref.6

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme oxidation Inferred from electronic annotation. Source: InterPro photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from direct assay PubMed 10072395. Source: TAIR
Molecular_function	heme binding Inferred from direct assay Ref.6. Source: TAIR heme oxygenase (decyclizing) activity Inferred from direct assay Ref.5Ref.6. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C9L4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9C9L4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     220-227: VSKKILDN → LCRYLRRY
     228-285: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast Potential
	Chain	59 – 285	227	Heme oxygenase 3, chloroplastic		PRO_0000412187
Sites
	Metal binding	89	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	220 – 227	8	VSKKILDN → LCRYLRRY in isoform 2.		VSP_041653
	Alternative sequence	228 – 285	58	Missing in isoform 2.		VSP_041654

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D552E638E7DFF241	FASTA	285	32,434
	10 20 30 40 50 60 MATTRLNPSC HFPASTRLSC ESYLGLRTTG RISYARTLTA PRGYLAVKAN GGQASVVTAA 70 80 90 100 110 120 AITEKQQKKY PGESKGFVEE MRFVAMRLHT KDQAREGEKE SRSPEEGPVA KWEPTVEGYL 130 140 150 160 170 180 HFLVDSKLVY DTLEGIIDGS NFPTYAGFKN TGLERAESLR KDLEWFKEQG YEIPEPMAPG 190 200 210 220 230 240 KTYSEYLKDL AENDPQAFIC HFYNIYFAHS AGGQMIGTKV SKKILDNKEL EFYKWDGQLS 250 260 270 280 QLLQNVRQKL NKVAEWWTRE EKSHCLEETE KSFKFSGEIL RLILS
	Isoform 2 [UniParc]. Checksum: DC1F3852838F4265	FASTA	227	25,607
	10 20 30 40 50 60 MATTRLNPSC HFPASTRLSC ESYLGLRTTG RISYARTLTA PRGYLAVKAN GGQASVVTAA 70 80 90 100 110 120 AITEKQQKKY PGESKGFVEE MRFVAMRLHT KDQAREGEKE SRSPEEGPVA KWEPTVEGYL 130 140 150 160 170 180 HFLVDSKLVY DTLEGIIDGS NFPTYAGFKN TGLERAESLR KDLEWFKEQG YEIPEPMAPG 190 200 210 220 KTYSEYLKDL AENDPQAFIC HFYNIYFAHS AGGQMIGTKL CRYLRRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants." Davis S.J., Bhoo S.H., Durski A.M., Walker J.M., Vierstra R.D. Plant Physiol. 126:656-669(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis." Emborg T.J., Walker J.M., Noh B., Vierstra R.D. Plant Physiol. 140:856-868(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions." Gisk B., Yasui Y., Kohchi T., Frankenberg-Dinkel N. Biochem. J. 425:425-434(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF320022 Genomic DNA. Translation: AAK63006.1. AC013289 Genomic DNA. Translation: AAG52552.1. CP002684 Genomic DNA. Translation: AEE34966.1. CP002684 Genomic DNA. Translation: AEE34967.1. AY084250 mRNA. Translation: AAM60844.1.
IPI	IPI00548070. IPI00891017.
PIR	B96719.
RefSeq	NP_001117574.1. NM_001124102.1. NP_177130.1. NM_105640.3.
UniGene	At.35382. At.71252.
3D structure databases
ProteinModelPortal	Q9C9L4.
SMR	Q9C9L4. Positions 153-280.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G69720.1-P.
Proteomic databases
PRIDE	Q9C9L4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	843308.
KEGG	ath:AT1G69720.
Organism-specific databases
TAIR	At1g69720.
Phylogenomic databases
eggNOG	NOG308332.
HOGENOM	HOG000265822.
InParanoid	Q9C9L4.
KO	K00510.
OMA	ISYARTL.
PhylomeDB	Q9C9L4.
ProtClustDB	CLSN2682571.
Gene expression databases
Genevestigator	Q9C9L4.
Family and domain databases
Gene3D	1.20.910.10. 1 hit.
InterPro	IPR016053. Haem_Oase-like. IPR016084. Haem_Oase-like_multi-hlx. IPR016951. Haem_Oase_decyc_pln. [Graphical view]
Pfam	PF01126. Heme_oxygenase. 1 hit. [Graphical view]
PIRSF	PIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAM	SSF48613. Heme_oxygenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HMOX3_ARATH

Accession

Primary (citable) accession number: Q9C9L4
Secondary accession number(s): B3H531

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program