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Q9C9L4 (HMOX3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heme oxygenase 3, chloroplastic

EC=1.14.99.3
Gene names
Name:HO3
Ordered Locus Names:At1g69720
ORF Names:T6C23.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Plays a minor role in phytochrome assembly and photomorphogenesis. Ref.5 Ref.6

Catalytic activity

Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

Subcellular location

Plastidchloroplast Ref.6.

Tissue specificity

Widely expressed at low levels. Ref.5

Disruption phenotype

No visible phenotype under normal growth conditions. Ref.5

Sequence similarities

Belongs to the heme oxygenase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0. Ref.6

Ontologies

Keywords
   Biological processPhotosynthesis
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processheme oxidation

Inferred from electronic annotation. Source: InterPro

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from direct assay PubMed 10072395. Source: TAIR

   Molecular_functionheme binding

Inferred from direct assay Ref.6. Source: TAIR

heme oxygenase (decyclizing) activity

Inferred from direct assay Ref.5Ref.6. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9C9L4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9C9L4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     220-227: VSKKILDN → LCRYLRRY
     228-285: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5858Chloroplast Potential
Chain59 – 285227Heme oxygenase 3, chloroplastic
PRO_0000412187

Sites

Metal binding891Iron (heme axial ligand) By similarity

Natural variations

Alternative sequence220 – 2278VSKKILDN → LCRYLRRY in isoform 2.
VSP_041653
Alternative sequence228 – 28558Missing in isoform 2.
VSP_041654

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D552E638E7DFF241

FASTA28532,434
        10         20         30         40         50         60 
MATTRLNPSC HFPASTRLSC ESYLGLRTTG RISYARTLTA PRGYLAVKAN GGQASVVTAA 

        70         80         90        100        110        120 
AITEKQQKKY PGESKGFVEE MRFVAMRLHT KDQAREGEKE SRSPEEGPVA KWEPTVEGYL 

       130        140        150        160        170        180 
HFLVDSKLVY DTLEGIIDGS NFPTYAGFKN TGLERAESLR KDLEWFKEQG YEIPEPMAPG 

       190        200        210        220        230        240 
KTYSEYLKDL AENDPQAFIC HFYNIYFAHS AGGQMIGTKV SKKILDNKEL EFYKWDGQLS 

       250        260        270        280 
QLLQNVRQKL NKVAEWWTRE EKSHCLEETE KSFKFSGEIL RLILS 

« Hide

Isoform 2 [UniParc].

Checksum: DC1F3852838F4265
Show »

FASTA22725,607

References

« Hide 'large scale' references
[1]"The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants."
Davis S.J., Bhoo S.H., Durski A.M., Walker J.M., Vierstra R.D.
Plant Physiol. 126:656-669(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis."
Emborg T.J., Walker J.M., Noh B., Vierstra R.D.
Plant Physiol. 140:856-868(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]"Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions."
Gisk B., Yasui Y., Kohchi T., Frankenberg-Dinkel N.
Biochem. J. 425:425-434(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF320022 Genomic DNA. Translation: AAK63006.1.
AC013289 Genomic DNA. Translation: AAG52552.1.
CP002684 Genomic DNA. Translation: AEE34966.1.
CP002684 Genomic DNA. Translation: AEE34967.1.
AY084250 mRNA. Translation: AAM60844.1.
PIRB96719.
RefSeqNP_001117574.1. NM_001124102.1. [Q9C9L4-2]
NP_177130.1. NM_105640.3. [Q9C9L4-1]
UniGeneAt.35382.
At.71252.

3D structure databases

ProteinModelPortalQ9C9L4.
SMRQ9C9L4. Positions 153-280.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G69720.1-P.

Proteomic databases

PRIDEQ9C9L4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G69720.1; AT1G69720.1; AT1G69720. [Q9C9L4-1]
GeneID843308.
KEGGath:AT1G69720.

Organism-specific databases

TAIRAT1G69720.

Phylogenomic databases

eggNOGNOG308332.
HOGENOMHOG000265822.
InParanoidQ9C9L4.
KOK00510.
OMAHIMANSG.
PhylomeDBQ9C9L4.

Enzyme and pathway databases

BioCycARA:AT1G69720-MONOMER.
MetaCyc:AT1G69720-MONOMER.

Gene expression databases

GenevestigatorQ9C9L4.

Family and domain databases

Gene3D1.20.910.10. 1 hit.
InterProIPR016053. Haem_Oase-like.
IPR016084. Haem_Oase-like_multi-hlx.
IPR016951. Haem_Oase_decyc_pln.
[Graphical view]
PfamPF01126. Heme_oxygenase. 1 hit.
[Graphical view]
PIRSFPIRSF030219. Heme_Oase_decyc_pln. 1 hit.
SUPFAMSSF48613. SSF48613. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHMOX3_ARATH
AccessionPrimary (citable) accession number: Q9C9L4
Secondary accession number(s): B3H531
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names