ID   GUN9_ARATH              Reviewed;         484 AA.
AC   Q9C9H5; P80855; Q38817; Q8LDE8;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 140.
DE   RecName: Full=Endoglucanase 9;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase 3;
DE            Short=AtCEL3;
DE   AltName: Full=Endo-1,4-beta glucanase 9;
DE   Flags: Precursor;
GN   Name=CEL3; OrderedLocusNames=At1g71380; ORFNames=F26A9.24;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1] {ECO:0000312|EMBL:AAG51817.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia {ECO:0000312|EMBL:AAG51817.1};
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2] {ECO:0000312|EMBL:AAM63253.1}
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3] {ECO:0000312|EMBL:AAM63253.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 5-484.
RC   STRAIN=cv. Columbia;
RA   Lu G., Ferl R.J.;
RT   "An Arabidopsis cDNA encoding beta-glucanase.";
RL   Plant Mol. Biol. 29:883-883(1995).
RN   [5] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 22-40, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=9188482; DOI=10.1074/jbc.272.25.15841;
RA   Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P.,
RA   Slabas A.R.;
RT   "Differential extraction and protein sequencing reveals major differences
RT   in patterns of primary cell wall proteins from plants.";
RL   J. Biol. Chem. 272:15841-15848(1997).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL   J. Mol. Evol. 58:506-515(2004).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=15604746; DOI=10.1007/s11103-004-3380-3;
RA   del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.;
RT   "Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase):
RT   a new marker to study root development in Arabidopsis.";
RL   Plant Mol. Biol. 56:309-323(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:9188482}.
CC   -!- TISSUE SPECIFICITY: Specifically expressed in root cap cells.
CC       {ECO:0000269|PubMed:15604746}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA90944.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AC016163; AAG51817.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE35195.1; -; Genomic_DNA.
DR   EMBL; AY086043; AAM63253.1; -; mRNA.
DR   EMBL; U17888; AAA90944.1; ALT_FRAME; mRNA.
DR   PIR; S61430; S61430.
DR   RefSeq; NP_177294.1; NM_105807.2.
DR   AlphaFoldDB; Q9C9H5; -.
DR   SMR; Q9C9H5; -.
DR   BioGRID; 28699; 8.
DR   IntAct; Q9C9H5; 17.
DR   STRING; 3702.Q9C9H5; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; 3702-AT1G71380-1; -.
DR   ProteomicsDB; 247320; -.
DR   EnsemblPlants; AT1G71380.1; AT1G71380.1; AT1G71380.
DR   GeneID; 843479; -.
DR   Gramene; AT1G71380.1; AT1G71380.1; AT1G71380.
DR   KEGG; ath:AT1G71380; -.
DR   Araport; AT1G71380; -.
DR   TAIR; AT1G71380; CEL3.
DR   eggNOG; ENOG502QRXS; Eukaryota.
DR   HOGENOM; CLU_008926_1_2_1; -.
DR   InParanoid; Q9C9H5; -.
DR   OMA; GGFQPFF; -.
DR   OrthoDB; 1347382at2759; -.
DR   PhylomeDB; Q9C9H5; -.
DR   BioCyc; ARA:AT1G71380-MONOMER; -.
DR   BRENDA; 3.2.1.4; 399.
DR   PRO; PR:Q9C9H5; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C9H5; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR   GO; GO:0009505; C:plant-type cell wall; IDA:UniProtKB.
DR   GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF54; ENDOGLUCANASE 9; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
DR   Genevisible; Q9C9H5; AT.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cell wall; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:9188482"
FT   CHAIN           22..484
FT                   /note="Endoglucanase 9"
FT                   /id="PRO_0000043221"
FT   ACT_SITE        77
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        402
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        453
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        462
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CONFLICT        135
FT                   /note="G -> A (in Ref. 4; AAA90944)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        173
FT                   /note="E -> K (in Ref. 4; AAA90944)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        189
FT                   /note="D -> N (in Ref. 3; AAM63253)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   484 AA;  53274 MW;  227D3945FCD2AF39 CRC64;
     MTSLFFFVLL FSSLLISNGD ANPNYKEALS KSLLFFQGQR SGPLPRGQQI SWRASSGLSD
     GSAAHVDLTG GYYDAGDNVK FNLPMAFTTT MLSWSALEYG KRMGPELENA RVNIRWATDY
     LLKCARATPG KLYVGVGDPN VDHKCWERPE DMDTPRTVYS VSASNPGSDV AAETAAALAA
     ASMVFRKVDS KYSRLLLATA KDVMQFAIQY QGAYSDSLSS SVCPFYCSYS GYKDELMWGA
     SWLLRATNNP YYANFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRALLNKD SNFEQYKQAA
     ENFICKILPD SPSSSTQYTQ GGLMYKLPQS NLQYVTSITF LLTTYAKYMK ATKHTFNCGS
     SVIVPNALIS LSKRQVDYIL GDNPIKMSYM VGFSSNFPKR IHHRASSLPS HALRSQSLGC
     NGGFQSFYTQ NPNPNILTGA IVGGPNQNDG YPDQRDDYSH AEPATYINAA FVGPLAYFAA
     GRST
//