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Q9C9H5

- GUN9_ARATH

UniProt

Q9C9H5 - GUN9_ARATH

Protein

Endoglucanase 9

Gene

CEL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei402 – 4021By similarityPROSITE-ProRule annotation
    Active sitei453 – 4531By similarityPROSITE-ProRule annotation
    Active sitei462 – 4621By similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciARA:AT1G71380-MONOMER.

    Protein family/group databases

    CAZyiGH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 9 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase 3
    Short name:
    AtCEL3
    Endo-1,4-beta glucanase 9
    Gene namesi
    Name:CEL3
    Ordered Locus Names:At1g71380
    ORF Names:F26A9.24
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G71380.

    Subcellular locationi

    Secretedcell wall 1 Publication

    GO - Cellular componenti

    1. cell wall Source: UniProtKB
    2. extracellular region Source: UniProtKB-KW
    3. Golgi apparatus Source: TAIR
    4. plant-type cell wall Source: TAIR
    5. plasma membrane Source: TAIR
    6. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 21211 PublicationAdd
    BLAST
    Chaini22 – 484463Endoglucanase 9PRO_0000043221Add
    BLAST

    Proteomic databases

    PaxDbiQ9C9H5.
    PRIDEiQ9C9H5.

    Expressioni

    Tissue specificityi

    Specifically expressed in root cap cells.1 Publication

    Gene expression databases

    ArrayExpressiQ9C9H5.
    GenevestigatoriQ9C9H5.

    Interactioni

    Protein-protein interaction databases

    BioGridi28699. 8 interactions.
    IntActiQ9C9H5. 16 interactions.
    STRINGi3702.AT1G71380.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C9H5.
    SMRiQ9C9H5. Positions 24-476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 9 (cellulase E) family.Sequence Analysis

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG249374.
    HOGENOMiHOG000021033.
    InParanoidiQ9C9H5.
    OMAiENFICKI.
    PhylomeDBiQ9C9H5.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9C9H5-1 [UniParc]FASTAAdd to Basket

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    MTSLFFFVLL FSSLLISNGD ANPNYKEALS KSLLFFQGQR SGPLPRGQQI    50
    SWRASSGLSD GSAAHVDLTG GYYDAGDNVK FNLPMAFTTT MLSWSALEYG 100
    KRMGPELENA RVNIRWATDY LLKCARATPG KLYVGVGDPN VDHKCWERPE 150
    DMDTPRTVYS VSASNPGSDV AAETAAALAA ASMVFRKVDS KYSRLLLATA 200
    KDVMQFAIQY QGAYSDSLSS SVCPFYCSYS GYKDELMWGA SWLLRATNNP 250
    YYANFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRALLNKD SNFEQYKQAA 300
    ENFICKILPD SPSSSTQYTQ GGLMYKLPQS NLQYVTSITF LLTTYAKYMK 350
    ATKHTFNCGS SVIVPNALIS LSKRQVDYIL GDNPIKMSYM VGFSSNFPKR 400
    IHHRASSLPS HALRSQSLGC NGGFQSFYTQ NPNPNILTGA IVGGPNQNDG 450
    YPDQRDDYSH AEPATYINAA FVGPLAYFAA GRST 484
    Length:484
    Mass (Da):53,274
    Last modified:June 1, 2001 - v1
    Checksum:i227D3945FCD2AF39
    GO

    Sequence cautioni

    The sequence AAA90944.1 differs from that shown. Reason: Frameshift at position 292.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti135 – 1351G → A in AAA90944. 1 PublicationCurated
    Sequence conflicti173 – 1731E → K in AAA90944. 1 PublicationCurated
    Sequence conflicti189 – 1891D → N in AAM63253. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC016163 Genomic DNA. Translation: AAG51817.1.
    CP002684 Genomic DNA. Translation: AEE35195.1.
    AY086043 mRNA. Translation: AAM63253.1.
    U17888 mRNA. Translation: AAA90944.1. Frameshift.
    PIRiS61430.
    RefSeqiNP_177294.1. NM_105807.1.
    UniGeneiAt.1677.

    Genome annotation databases

    EnsemblPlantsiAT1G71380.1; AT1G71380.1; AT1G71380.
    GeneIDi843479.
    KEGGiath:AT1G71380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC016163 Genomic DNA. Translation: AAG51817.1 .
    CP002684 Genomic DNA. Translation: AEE35195.1 .
    AY086043 mRNA. Translation: AAM63253.1 .
    U17888 mRNA. Translation: AAA90944.1 . Frameshift.
    PIRi S61430.
    RefSeqi NP_177294.1. NM_105807.1.
    UniGenei At.1677.

    3D structure databases

    ProteinModelPortali Q9C9H5.
    SMRi Q9C9H5. Positions 24-476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28699. 8 interactions.
    IntActi Q9C9H5. 16 interactions.
    STRINGi 3702.AT1G71380.1-P.

    Protein family/group databases

    CAZyi GH9. Glycoside Hydrolase Family 9.

    Proteomic databases

    PaxDbi Q9C9H5.
    PRIDEi Q9C9H5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G71380.1 ; AT1G71380.1 ; AT1G71380 .
    GeneIDi 843479.
    KEGGi ath:AT1G71380.

    Organism-specific databases

    TAIRi AT1G71380.

    Phylogenomic databases

    eggNOGi NOG249374.
    HOGENOMi HOG000021033.
    InParanoidi Q9C9H5.
    OMAi ENFICKI.
    PhylomeDBi Q9C9H5.

    Enzyme and pathway databases

    BioCyci ARA:AT1G71380-MONOMER.

    Gene expression databases

    ArrayExpressi Q9C9H5.
    Genevestigatori Q9C9H5.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. ColumbiaImported.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "An Arabidopsis cDNA encoding beta-glucanase."
      Lu G., Ferl R.J.
      Plant Mol. Biol. 29:883-883(1995)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-484.
      Strain: cv. Columbia.
    5. "Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants."
      Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R.
      J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-40, SUBCELLULAR LOCATION.
      Strain: cv. Landsberg erecta.
    6. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
      Libertini E., Li Y., McQueen-Mason S.J.
      J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    7. "Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis."
      del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.
      Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGUN9_ARATH
    AccessioniPrimary (citable) accession number: Q9C9H5
    Secondary accession number(s): P80855, Q38817, Q8LDE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3