Q9C9H5 (GUN9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 9
EC=3.2.1.4

Alternative name(s):
Cellulase 3
Short name=AtCEL3
Endo-1,4-beta glucanase 9

Gene names

Name:	CEL3
Ordered Locus Names:	At1g71380
ORF Names:	F26A9.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted › cell wall Ref.5.
Tissue specificity	Specifically expressed in root cap cells. Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence AAA90944.1 differs from that shown. Reason: Frameshift at position 292.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plant-type cell wall Inferred from direct assay PubMed 16287169. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.5
	Chain	22 – 484	463	Endoglucanase 9 Ref.5		PRO_0000043221
Sites
	Active site	402	1	By similarity UniProtKB P05522
	Active site	453	1	By similarity UniProtKB P05522
	Active site	462	1	By similarity UniProtKB P05522
Experimental info
	Sequence conflict	135	1	G → A in AAA90944. Ref.4
	Sequence conflict	173	1	E → K in AAA90944. Ref.4
	Sequence conflict	189	1	D → N in AAM63253. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9C9H5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 227D3945FCD2AF39	FASTA	484	53,274
	10 20 30 40 50 60 MTSLFFFVLL FSSLLISNGD ANPNYKEALS KSLLFFQGQR SGPLPRGQQI SWRASSGLSD 70 80 90 100 110 120 GSAAHVDLTG GYYDAGDNVK FNLPMAFTTT MLSWSALEYG KRMGPELENA RVNIRWATDY 130 140 150 160 170 180 LLKCARATPG KLYVGVGDPN VDHKCWERPE DMDTPRTVYS VSASNPGSDV AAETAAALAA 190 200 210 220 230 240 ASMVFRKVDS KYSRLLLATA KDVMQFAIQY QGAYSDSLSS SVCPFYCSYS GYKDELMWGA 250 260 270 280 290 300 SWLLRATNNP YYANFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRALLNKD SNFEQYKQAA 310 320 330 340 350 360 ENFICKILPD SPSSSTQYTQ GGLMYKLPQS NLQYVTSITF LLTTYAKYMK ATKHTFNCGS 370 380 390 400 410 420 SVIVPNALIS LSKRQVDYIL GDNPIKMSYM VGFSSNFPKR IHHRASSLPS HALRSQSLGC 430 440 450 460 470 480 NGGFQSFYTQ NPNPNILTGA IVGGPNQNDG YPDQRDDYSH AEPATYINAA FVGPLAYFAA GRST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"An Arabidopsis cDNA encoding beta-glucanase." Lu G., Ferl R.J. Plant Mol. Biol. 29:883-883(1995) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-484. Strain: cv. Columbia.
[5]	"Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants." Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R. J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-40, SUBCELLULAR LOCATION. Strain: cv. Landsberg erecta.
[6]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[7]	"Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis." del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E. Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC016163 Genomic DNA. Translation: AAG51817.1. CP002684 Genomic DNA. Translation: AEE35195.1. AY086043 mRNA. Translation: AAM63253.1. U17888 mRNA. Translation: AAA90944.1. Frameshift.
IPI	IPI00526368.
PIR	S61430.
RefSeq	NP_177294.1. NM_105807.1.
UniGene	At.1677.
3D structure databases
ProteinModelPortal	Q9C9H5.
SMR	Q9C9H5. Positions 24-476.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C9H5. 16 interactions.
STRING	3702.AT1G71380.1-P.
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PaxDb	Q9C9H5.
PRIDE	Q9C9H5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G71380.1; AT1G71380.1; AT1G71380.
GeneID	843479.
KEGG	ath:AT1G71380.
Organism-specific databases
TAIR	At1g71380.
Phylogenomic databases
eggNOG	NOG249374.
HOGENOM	HOG000021033.
InParanoid	Q9C9H5.
OMA	ENARVNI.
PhylomeDB	Q9C9H5.
ProtClustDB	PLN02175.
Gene expression databases
ArrayExpress	Q9C9H5.
Genevestigator	Q9C9H5.
GermOnline	AT1G71380. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN9_ARATH

Accession

Primary (citable) accession number: Q9C9H5
Secondary accession number(s): P80855, Q38817, Q8LDE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program