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Q9C9H5 (GUN9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 9

EC=3.2.1.4
Alternative name(s):
Cellulase 3
Short name=AtCEL3
Endo-1,4-beta glucanase 9
Gene names
Name:CEL3
Ordered Locus Names:At1g71380
ORF Names:F26A9.24
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secretedcell wall Ref.5.

Tissue specificity

Specifically expressed in root cap cells. Ref.7

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Sequence caution

The sequence AAA90944.1 differs from that shown. Reason: Frameshift at position 292.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.5
Chain22 – 484463Endoglucanase 9 Ref.5
PRO_0000043221

Sites

Active site4021 By similarity UniProtKB P05522
Active site4531 By similarity UniProtKB P05522
Active site4621 By similarity UniProtKB P05522

Experimental info

Sequence conflict1351G → A in AAA90944. Ref.4
Sequence conflict1731E → K in AAA90944. Ref.4
Sequence conflict1891D → N in AAM63253. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9C9H5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 227D3945FCD2AF39

FASTA48453,274
        10         20         30         40         50         60 
MTSLFFFVLL FSSLLISNGD ANPNYKEALS KSLLFFQGQR SGPLPRGQQI SWRASSGLSD 

        70         80         90        100        110        120 
GSAAHVDLTG GYYDAGDNVK FNLPMAFTTT MLSWSALEYG KRMGPELENA RVNIRWATDY 

       130        140        150        160        170        180 
LLKCARATPG KLYVGVGDPN VDHKCWERPE DMDTPRTVYS VSASNPGSDV AAETAAALAA 

       190        200        210        220        230        240 
ASMVFRKVDS KYSRLLLATA KDVMQFAIQY QGAYSDSLSS SVCPFYCSYS GYKDELMWGA 

       250        260        270        280        290        300 
SWLLRATNNP YYANFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRALLNKD SNFEQYKQAA 

       310        320        330        340        350        360 
ENFICKILPD SPSSSTQYTQ GGLMYKLPQS NLQYVTSITF LLTTYAKYMK ATKHTFNCGS 

       370        380        390        400        410        420 
SVIVPNALIS LSKRQVDYIL GDNPIKMSYM VGFSSNFPKR IHHRASSLPS HALRSQSLGC 

       430        440        450        460        470        480 
NGGFQSFYTQ NPNPNILTGA IVGGPNQNDG YPDQRDDYSH AEPATYINAA FVGPLAYFAA 


GRST 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"An Arabidopsis cDNA encoding beta-glucanase."
Lu G., Ferl R.J.
Plant Mol. Biol. 29:883-883(1995)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-484.
Strain: cv. Columbia.
[5]"Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants."
Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R.
J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-40, SUBCELLULAR LOCATION.
Strain: cv. Landsberg erecta.
[6]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis."
del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.
Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC016163 Genomic DNA. Translation: AAG51817.1.
CP002684 Genomic DNA. Translation: AEE35195.1.
AY086043 mRNA. Translation: AAM63253.1.
U17888 mRNA. Translation: AAA90944.1. Frameshift.
PIRS61430.
RefSeqNP_177294.1. NM_105807.1.
UniGeneAt.1677.

3D structure databases

ProteinModelPortalQ9C9H5.
SMRQ9C9H5. Positions 24-476.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid28699. 8 interactions.
IntActQ9C9H5. 16 interactions.
STRING3702.AT1G71380.1-P.

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbQ9C9H5.
PRIDEQ9C9H5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G71380.1; AT1G71380.1; AT1G71380.
GeneID843479.
KEGGath:AT1G71380.

Organism-specific databases

TAIRAT1G71380.

Phylogenomic databases

eggNOGNOG249374.
HOGENOMHOG000021033.
InParanoidQ9C9H5.
OMAENFICKI.
PhylomeDBQ9C9H5.

Enzyme and pathway databases

BioCycARA:AT1G71380-MONOMER.

Gene expression databases

ArrayExpressQ9C9H5.
GenevestigatorQ9C9H5.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN9_ARATH
AccessionPrimary (citable) accession number: Q9C9H5
Secondary accession number(s): P80855, Q38817, Q8LDE8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names