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Protein

Endoglucanase 9

Gene

CEL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei402 – 4021By similarityPROSITE-ProRule annotation
Active sitei453 – 4531By similarityPROSITE-ProRule annotation
Active sitei462 – 4621By similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT1G71380-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 9 (EC:3.2.1.4)
Alternative name(s):
Cellulase 3
Short name:
AtCEL3
Endo-1,4-beta glucanase 9
Gene namesi
Name:CEL3
Ordered Locus Names:At1g71380
ORF Names:F26A9.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G71380.

Subcellular locationi

Secretedcell wall 1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB
  2. extracellular region Source: UniProtKB-KW
  3. Golgi apparatus Source: TAIR
  4. plant-type cell wall Source: TAIR
  5. plasma membrane Source: TAIR
  6. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 484463Endoglucanase 9PRO_0000043221Add
BLAST

Proteomic databases

PaxDbiQ9C9H5.
PRIDEiQ9C9H5.

Expressioni

Tissue specificityi

Specifically expressed in root cap cells.1 Publication

Gene expression databases

ExpressionAtlasiQ9C9H5. baseline and differential.
GenevestigatoriQ9C9H5.

Interactioni

Protein-protein interaction databases

BioGridi28699. 8 interactions.
IntActiQ9C9H5. 16 interactions.
STRINGi3702.AT1G71380.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C9H5.
SMRiQ9C9H5. Positions 24-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 9 (cellulase E) family.Sequence Analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG249374.
HOGENOMiHOG000021033.
InParanoidiQ9C9H5.
OMAiHTFSCQS.
PhylomeDBiQ9C9H5.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C9H5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSLFFFVLL FSSLLISNGD ANPNYKEALS KSLLFFQGQR SGPLPRGQQI
60 70 80 90 100
SWRASSGLSD GSAAHVDLTG GYYDAGDNVK FNLPMAFTTT MLSWSALEYG
110 120 130 140 150
KRMGPELENA RVNIRWATDY LLKCARATPG KLYVGVGDPN VDHKCWERPE
160 170 180 190 200
DMDTPRTVYS VSASNPGSDV AAETAAALAA ASMVFRKVDS KYSRLLLATA
210 220 230 240 250
KDVMQFAIQY QGAYSDSLSS SVCPFYCSYS GYKDELMWGA SWLLRATNNP
260 270 280 290 300
YYANFIKSLG GGDQPDIFSW DNKYAGAYVL LSRRALLNKD SNFEQYKQAA
310 320 330 340 350
ENFICKILPD SPSSSTQYTQ GGLMYKLPQS NLQYVTSITF LLTTYAKYMK
360 370 380 390 400
ATKHTFNCGS SVIVPNALIS LSKRQVDYIL GDNPIKMSYM VGFSSNFPKR
410 420 430 440 450
IHHRASSLPS HALRSQSLGC NGGFQSFYTQ NPNPNILTGA IVGGPNQNDG
460 470 480
YPDQRDDYSH AEPATYINAA FVGPLAYFAA GRST
Length:484
Mass (Da):53,274
Last modified:June 1, 2001 - v1
Checksum:i227D3945FCD2AF39
GO

Sequence cautioni

The sequence AAA90944.1 differs from that shown. Reason: Frameshift at position 292. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 1351G → A in AAA90944. 1 PublicationCurated
Sequence conflicti173 – 1731E → K in AAA90944. 1 PublicationCurated
Sequence conflicti189 – 1891D → N in AAM63253. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016163 Genomic DNA. Translation: AAG51817.1.
CP002684 Genomic DNA. Translation: AEE35195.1.
AY086043 mRNA. Translation: AAM63253.1.
U17888 mRNA. Translation: AAA90944.1. Frameshift.
PIRiS61430.
RefSeqiNP_177294.1. NM_105807.1.
UniGeneiAt.1677.

Genome annotation databases

EnsemblPlantsiAT1G71380.1; AT1G71380.1; AT1G71380.
GeneIDi843479.
KEGGiath:AT1G71380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016163 Genomic DNA. Translation: AAG51817.1.
CP002684 Genomic DNA. Translation: AEE35195.1.
AY086043 mRNA. Translation: AAM63253.1.
U17888 mRNA. Translation: AAA90944.1. Frameshift.
PIRiS61430.
RefSeqiNP_177294.1. NM_105807.1.
UniGeneiAt.1677.

3D structure databases

ProteinModelPortaliQ9C9H5.
SMRiQ9C9H5. Positions 24-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28699. 8 interactions.
IntActiQ9C9H5. 16 interactions.
STRINGi3702.AT1G71380.1-P.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbiQ9C9H5.
PRIDEiQ9C9H5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G71380.1; AT1G71380.1; AT1G71380.
GeneIDi843479.
KEGGiath:AT1G71380.

Organism-specific databases

TAIRiAT1G71380.

Phylogenomic databases

eggNOGiNOG249374.
HOGENOMiHOG000021033.
InParanoidiQ9C9H5.
OMAiHTFSCQS.
PhylomeDBiQ9C9H5.

Enzyme and pathway databases

BioCyciARA:AT1G71380-MONOMER.

Gene expression databases

ExpressionAtlasiQ9C9H5. baseline and differential.
GenevestigatoriQ9C9H5.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. ColumbiaImported.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "An Arabidopsis cDNA encoding beta-glucanase."
    Lu G., Ferl R.J.
    Plant Mol. Biol. 29:883-883(1995)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-484.
    Strain: cv. Columbia.
  5. "Differential extraction and protein sequencing reveals major differences in patterns of primary cell wall proteins from plants."
    Robertson D., Mitchell G.P., Gilroy J.S., Gerrish C., Bolwell G.P., Slabas A.R.
    J. Biol. Chem. 272:15841-15848(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-40, SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta.
  6. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
    Libertini E., Li Y., McQueen-Mason S.J.
    J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "Root cap specific expression of an endo-beta-1,4-D-glucanase (cellulase): a new marker to study root development in Arabidopsis."
    del Campillo E., Abdel-Aziz A., Crawford D., Patterson S.E.
    Plant Mol. Biol. 56:309-323(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGUN9_ARATH
AccessioniPrimary (citable) accession number: Q9C9H5
Secondary accession number(s): P80855, Q38817, Q8LDE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.