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Protein

Endonuclease 2

Gene

ENDO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable endonuclease (By similarity). Cannot hydrolyze single-stranded DNA and does not cleave mismatches.By similarity1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Zn2+By similarityNote: Binds 3 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Zinc 3By similarity1
Metal bindingi33Zinc 3By similarity1
Metal bindingi72Zinc 1By similarity1
Metal bindingi85Zinc 1By similarity1
Metal bindingi147Zinc 1By similarity1
Metal bindingi151Zinc 1By similarity1
Metal bindingi151Zinc 3By similarity1
Metal bindingi157Zinc 2By similarity1
Metal bindingi181Zinc 2By similarity1
Metal bindingi185Zinc 2By similarity1

GO - Molecular functioni

  • endonuclease activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 2 (EC:3.1.30.1)
Short name:
AtENDO2
Alternative name(s):
Deoxyribonuclease ENDO2
Single-stranded-nucleate endonuclease ENDO2
Gene namesi
Name:ENDO2
Ordered Locus Names:At1g68290
ORF Names:T22E19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G68290.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27By similarityAdd BLAST27
ChainiPRO_000041762028 – 290Endonuclease 2Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi93 ↔ 245By similarity
Disulfide bondi101 ↔ 111By similarity
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Glycosylationi137N-linked (GlcNAc...)Sequence analysis1
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9C9G4.

Expressioni

Gene expression databases

GenevisibleiQ9C9G4. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G68290.1.

Structurei

Secondary structure

1290
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 42Combined sources13
Helixi46 – 55Combined sources10
Helixi58 – 60Combined sources3
Helixi64 – 67Combined sources4
Helixi70 – 74Combined sources5
Turni75 – 78Combined sources4
Helixi79 – 85Combined sources7
Beta strandi87 – 89Combined sources3
Beta strandi91 – 93Combined sources3
Helixi97 – 100Combined sources4
Helixi112 – 123Combined sources12
Turni124 – 127Combined sources4
Helixi138 – 151Combined sources14
Helixi155 – 157Combined sources3
Beta strandi158 – 160Combined sources3
Helixi161 – 163Combined sources3
Turni164 – 168Combined sources5
Beta strandi170 – 173Combined sources4
Beta strandi176 – 179Combined sources4
Helixi180 – 185Combined sources6
Helixi187 – 195Combined sources9
Turni196 – 199Combined sources4
Helixi203 – 214Combined sources12
Helixi217 – 224Combined sources8
Beta strandi228 – 230Combined sources3
Helixi233 – 246Combined sources14
Helixi260 – 289Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W52X-ray1.76A28-290[»]
4CWMX-ray2.09A/B28-290[»]
4CXOX-ray1.67A28-290[»]
4CXPX-ray1.22A28-290[»]
4CXVX-ray2.00A/B28-290[»]
ProteinModelPortaliQ9C9G4.
SMRiQ9C9G4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nuclease type I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF74. Eukaryota.
ENOG4111UE2. LUCA.
HOGENOMiHOG000032024.
InParanoidiQ9C9G4.
OMAiDWAYKGV.
OrthoDBiEOG09360JJA.
PhylomeDBiQ9C9G4.

Family and domain databases

CDDicd11010. S1-P1_nuclease. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR003154. S1/P1nuclease.
[Graphical view]
PfamiPF02265. S1-P1_nuclease. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C9G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANQKGLHVV MMIITVWLLY AAPNIHGWGK EGHEIICKIA QTRLDETAAK
60 70 80 90 100
AVKELLPESA EGDLSSLCLW ADRVKFRYHW SSPLHYINTP DACSYQYNRD
110 120 130 140 150
CKDESGEKGR CVAGAIYNYT TQLLSYKTAA SSQSQYNLTE ALLFVSHFMG
160 170 180 190 200
DIHQPLHVSY ASDKGGNTIE VHWYTRKANL HHIWDSNIIE TAEADLYNSA
210 220 230 240 250
LEGMVDALKK NITTEWADQV KRWETCTKKT ACPDIYASEG IQAACDWAYK
260 270 280 290
GVTEGDTLED EYFYSRLPIV YQRLAQGGVR LAATLNRIFG
Length:290
Mass (Da):32,745
Last modified:June 1, 2001 - v1
Checksum:i1FA734DAA2AC5736
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224E → D in AAM63596 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016447 Genomic DNA. Translation: AAG52597.1.
CP002684 Genomic DNA. Translation: AEE34777.1.
AY086531 mRNA. Translation: AAM63596.1.
PIRiE96706.
RefSeqiNP_176996.1. NM_105500.2.
UniGeneiAt.35544.
At.43330.

Genome annotation databases

EnsemblPlantsiAT1G68290.1; AT1G68290.1; AT1G68290.
GeneIDi843158.
GrameneiAT1G68290.1; AT1G68290.1; AT1G68290.
KEGGiath:AT1G68290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016447 Genomic DNA. Translation: AAG52597.1.
CP002684 Genomic DNA. Translation: AEE34777.1.
AY086531 mRNA. Translation: AAM63596.1.
PIRiE96706.
RefSeqiNP_176996.1. NM_105500.2.
UniGeneiAt.35544.
At.43330.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W52X-ray1.76A28-290[»]
4CWMX-ray2.09A/B28-290[»]
4CXOX-ray1.67A28-290[»]
4CXPX-ray1.22A28-290[»]
4CXVX-ray2.00A/B28-290[»]
ProteinModelPortaliQ9C9G4.
SMRiQ9C9G4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G68290.1.

Proteomic databases

PaxDbiQ9C9G4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G68290.1; AT1G68290.1; AT1G68290.
GeneIDi843158.
GrameneiAT1G68290.1; AT1G68290.1; AT1G68290.
KEGGiath:AT1G68290.

Organism-specific databases

TAIRiAT1G68290.

Phylogenomic databases

eggNOGiENOG410IF74. Eukaryota.
ENOG4111UE2. LUCA.
HOGENOMiHOG000032024.
InParanoidiQ9C9G4.
OMAiDWAYKGV.
OrthoDBiEOG09360JJA.
PhylomeDBiQ9C9G4.

Miscellaneous databases

PROiQ9C9G4.

Gene expression databases

GenevisibleiQ9C9G4. AT.

Family and domain databases

CDDicd11010. S1-P1_nuclease. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR003154. S1/P1nuclease.
[Graphical view]
PfamiPF02265. S1-P1_nuclease. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENDO2_ARATH
AccessioniPrimary (citable) accession number: Q9C9G4
Secondary accession number(s): Q8LCL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.