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Protein

Endonuclease 2

Gene

ENDO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable endonuclease (By similarity). Cannot hydrolyze single-stranded DNA and does not cleave mismatches.By similarity1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Zn2+By similarityNote: Binds 3 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281Zinc 3By similarity
Metal bindingi33 – 331Zinc 3By similarity
Metal bindingi72 – 721Zinc 1By similarity
Metal bindingi85 – 851Zinc 1By similarity
Metal bindingi147 – 1471Zinc 1By similarity
Metal bindingi151 – 1511Zinc 1By similarity
Metal bindingi151 – 1511Zinc 3By similarity
Metal bindingi157 – 1571Zinc 2By similarity
Metal bindingi181 – 1811Zinc 2By similarity
Metal bindingi185 – 1851Zinc 2By similarity

GO - Molecular functioni

  • endonuclease activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-1309-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 2 (EC:3.1.30.1)
Short name:
AtENDO2
Alternative name(s):
Deoxyribonuclease ENDO2
Single-stranded-nucleate endonuclease ENDO2
Gene namesi
Name:ENDO2
Ordered Locus Names:At1g68290
ORF Names:T22E19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G68290.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 290263Endonuclease 2PRO_0000417620Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi93 ↔ 245By similarity
Disulfide bondi101 ↔ 111By similarity
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9C9G4.
PRIDEiQ9C9G4.

Expressioni

Gene expression databases

GenevisibleiQ9C9G4. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G68290.1.

Structurei

Secondary structure

1
290
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 4213Combined sources
Helixi46 – 5510Combined sources
Helixi58 – 603Combined sources
Helixi64 – 674Combined sources
Helixi70 – 745Combined sources
Turni75 – 784Combined sources
Helixi79 – 857Combined sources
Beta strandi87 – 893Combined sources
Beta strandi91 – 933Combined sources
Helixi97 – 1004Combined sources
Helixi112 – 12312Combined sources
Turni124 – 1274Combined sources
Helixi138 – 15114Combined sources
Helixi155 – 1573Combined sources
Beta strandi158 – 1603Combined sources
Helixi161 – 1633Combined sources
Turni164 – 1685Combined sources
Beta strandi170 – 1734Combined sources
Beta strandi176 – 1794Combined sources
Helixi180 – 1856Combined sources
Helixi187 – 1959Combined sources
Turni196 – 1994Combined sources
Helixi203 – 21412Combined sources
Helixi217 – 2248Combined sources
Beta strandi228 – 2303Combined sources
Helixi233 – 24614Combined sources
Helixi260 – 28930Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W52X-ray1.76A28-290[»]
4CWMX-ray2.09A/B28-290[»]
4CXOX-ray1.67A28-290[»]
4CXPX-ray1.22A28-290[»]
4CXVX-ray2.00A/B28-290[»]
ProteinModelPortaliQ9C9G4.
SMRiQ9C9G4. Positions 28-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nuclease type I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF74. Eukaryota.
ENOG4111UE2. LUCA.
HOGENOMiHOG000032024.
InParanoidiQ9C9G4.
OMAiDWAYKGV.
OrthoDBiEOG09360JJA.
PhylomeDBiQ9C9G4.

Family and domain databases

CDDicd11010. S1-P1_nuclease. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR003154. S1/P1nuclease.
[Graphical view]
PfamiPF02265. S1-P1_nuclease. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C9G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANQKGLHVV MMIITVWLLY AAPNIHGWGK EGHEIICKIA QTRLDETAAK
60 70 80 90 100
AVKELLPESA EGDLSSLCLW ADRVKFRYHW SSPLHYINTP DACSYQYNRD
110 120 130 140 150
CKDESGEKGR CVAGAIYNYT TQLLSYKTAA SSQSQYNLTE ALLFVSHFMG
160 170 180 190 200
DIHQPLHVSY ASDKGGNTIE VHWYTRKANL HHIWDSNIIE TAEADLYNSA
210 220 230 240 250
LEGMVDALKK NITTEWADQV KRWETCTKKT ACPDIYASEG IQAACDWAYK
260 270 280 290
GVTEGDTLED EYFYSRLPIV YQRLAQGGVR LAATLNRIFG
Length:290
Mass (Da):32,745
Last modified:June 1, 2001 - v1
Checksum:i1FA734DAA2AC5736
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241E → D in AAM63596 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016447 Genomic DNA. Translation: AAG52597.1.
CP002684 Genomic DNA. Translation: AEE34777.1.
AY086531 mRNA. Translation: AAM63596.1.
PIRiE96706.
RefSeqiNP_176996.1. NM_105500.2.
UniGeneiAt.35544.
At.43330.

Genome annotation databases

EnsemblPlantsiAT1G68290.1; AT1G68290.1; AT1G68290.
GeneIDi843158.
GrameneiAT1G68290.1; AT1G68290.1; AT1G68290.
KEGGiath:AT1G68290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016447 Genomic DNA. Translation: AAG52597.1.
CP002684 Genomic DNA. Translation: AEE34777.1.
AY086531 mRNA. Translation: AAM63596.1.
PIRiE96706.
RefSeqiNP_176996.1. NM_105500.2.
UniGeneiAt.35544.
At.43330.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W52X-ray1.76A28-290[»]
4CWMX-ray2.09A/B28-290[»]
4CXOX-ray1.67A28-290[»]
4CXPX-ray1.22A28-290[»]
4CXVX-ray2.00A/B28-290[»]
ProteinModelPortaliQ9C9G4.
SMRiQ9C9G4. Positions 28-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G68290.1.

Proteomic databases

PaxDbiQ9C9G4.
PRIDEiQ9C9G4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G68290.1; AT1G68290.1; AT1G68290.
GeneIDi843158.
GrameneiAT1G68290.1; AT1G68290.1; AT1G68290.
KEGGiath:AT1G68290.

Organism-specific databases

TAIRiAT1G68290.

Phylogenomic databases

eggNOGiENOG410IF74. Eukaryota.
ENOG4111UE2. LUCA.
HOGENOMiHOG000032024.
InParanoidiQ9C9G4.
OMAiDWAYKGV.
OrthoDBiEOG09360JJA.
PhylomeDBiQ9C9G4.

Enzyme and pathway databases

BioCyciARA:GQT-1309-MONOMER.

Miscellaneous databases

PROiQ9C9G4.

Gene expression databases

GenevisibleiQ9C9G4. AT.

Family and domain databases

CDDicd11010. S1-P1_nuclease. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR003154. S1/P1nuclease.
[Graphical view]
PfamiPF02265. S1-P1_nuclease. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENDO2_ARATH
AccessioniPrimary (citable) accession number: Q9C9G4
Secondary accession number(s): Q8LCL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.