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Protein

Endonuclease 2

Gene

ENDO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable endonuclease (By similarity). Cannot hydrolyze single-stranded DNA and does not cleave mismatches.By similarity1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Zn2+By similarityNote: Binds 3 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Zinc 3By similarity1
Metal bindingi33Zinc 3By similarity1
Metal bindingi72Zinc 1By similarity1
Metal bindingi85Zinc 1By similarity1
Metal bindingi147Zinc 1By similarity1
Metal bindingi151Zinc 1By similarity1
Metal bindingi151Zinc 3By similarity1
Metal bindingi157Zinc 2By similarity1
Metal bindingi181Zinc 2By similarity1
Metal bindingi185Zinc 2By similarity1

GO - Molecular functioni

  • endonuclease activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 2 (EC:3.1.30.1)
Short name:
AtENDO2
Alternative name(s):
Deoxyribonuclease ENDO2
Single-stranded-nucleate endonuclease ENDO2
Gene namesi
Name:ENDO2
Ordered Locus Names:At1g68290
ORF Names:T22E19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G68290
TAIRilocus:2199297 AT1G68290

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27By similarityAdd BLAST27
ChainiPRO_000041762028 – 290Endonuclease 2Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi93 ↔ 245By similarity
Disulfide bondi101 ↔ 111By similarity
Glycosylationi118N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi137N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi211N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9C9G4
PRIDEiQ9C9G4

Expressioni

Gene expression databases

ExpressionAtlasiQ9C9G4 differential
GenevisibleiQ9C9G4 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G68290.1

Structurei

Secondary structure

1290
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 42Combined sources13
Helixi46 – 55Combined sources10
Helixi58 – 60Combined sources3
Helixi64 – 67Combined sources4
Helixi70 – 74Combined sources5
Turni75 – 78Combined sources4
Helixi79 – 85Combined sources7
Beta strandi87 – 89Combined sources3
Beta strandi91 – 93Combined sources3
Helixi97 – 100Combined sources4
Helixi112 – 123Combined sources12
Turni124 – 127Combined sources4
Helixi138 – 151Combined sources14
Helixi155 – 157Combined sources3
Beta strandi158 – 160Combined sources3
Helixi161 – 163Combined sources3
Turni164 – 168Combined sources5
Beta strandi170 – 173Combined sources4
Beta strandi176 – 179Combined sources4
Helixi180 – 185Combined sources6
Helixi187 – 195Combined sources9
Turni196 – 199Combined sources4
Helixi203 – 214Combined sources12
Helixi217 – 224Combined sources8
Beta strandi228 – 230Combined sources3
Helixi233 – 246Combined sources14
Helixi260 – 289Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W52X-ray1.76A28-290[»]
4CWMX-ray2.09A/B28-290[»]
4CXOX-ray1.67A28-290[»]
4CXPX-ray1.22A28-290[»]
4CXVX-ray2.00A/B28-290[»]
ProteinModelPortaliQ9C9G4
SMRiQ9C9G4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nuclease type I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF74 Eukaryota
ENOG4111UE2 LUCA
HOGENOMiHOG000032024
InParanoidiQ9C9G4
OMAiWGKTGHR
OrthoDBiEOG09360JJA
PhylomeDBiQ9C9G4

Family and domain databases

CDDicd11010 S1-P1_nuclease, 1 hit
Gene3Di1.10.575.10, 1 hit
InterProiView protein in InterPro
IPR008947 PLipase_C/P1_nuclease_dom_sf
IPR003154 S1/P1nuclease
PANTHERiPTHR33146 PTHR33146, 1 hit
PfamiView protein in Pfam
PF02265 S1-P1_nuclease, 1 hit
SUPFAMiSSF48537 SSF48537, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C9G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANQKGLHVV MMIITVWLLY AAPNIHGWGK EGHEIICKIA QTRLDETAAK
60 70 80 90 100
AVKELLPESA EGDLSSLCLW ADRVKFRYHW SSPLHYINTP DACSYQYNRD
110 120 130 140 150
CKDESGEKGR CVAGAIYNYT TQLLSYKTAA SSQSQYNLTE ALLFVSHFMG
160 170 180 190 200
DIHQPLHVSY ASDKGGNTIE VHWYTRKANL HHIWDSNIIE TAEADLYNSA
210 220 230 240 250
LEGMVDALKK NITTEWADQV KRWETCTKKT ACPDIYASEG IQAACDWAYK
260 270 280 290
GVTEGDTLED EYFYSRLPIV YQRLAQGGVR LAATLNRIFG
Length:290
Mass (Da):32,745
Last modified:June 1, 2001 - v1
Checksum:i1FA734DAA2AC5736
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224E → D in AAM63596 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016447 Genomic DNA Translation: AAG52597.1
CP002684 Genomic DNA Translation: AEE34777.1
AY086531 mRNA Translation: AAM63596.1
PIRiE96706
RefSeqiNP_176996.1, NM_105500.2
UniGeneiAt.35544
At.43330

Genome annotation databases

EnsemblPlantsiAT1G68290.1; AT1G68290.1; AT1G68290
GeneIDi843158
GrameneiAT1G68290.1; AT1G68290.1; AT1G68290
KEGGiath:AT1G68290

Similar proteinsi

Entry informationi

Entry nameiENDO2_ARATH
AccessioniPrimary (citable) accession number: Q9C9G4
Secondary accession number(s): Q8LCL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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