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Protein

Enolase 1, chloroplastic

Gene

ENO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.1 Publication

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Kineticsi

  1. KM=0.082 mM for 2-PGA (at pH 8)1 Publication
  2. KM=0.149 mM for 2-PGA (at pH 7)1 Publication
  3. KM=0.180 mM for PEP (at pH 8)1 Publication
  4. KM=0.534 mM for PEP (at pH 7)1 Publication
  5. KM=1.281 mM for 3-PGA (at pH 8)1 Publication
  6. KM=0.390 mM for 3-PGA (at pH 7)1 Publication

    pH dependencei

    Optimum pH is 7.5-8.5 for the forward reaction (PEP formation) and 6.0-7.5 for the reverse reaction (2-PGA formation).1 Publication

    Pathwayi: glycolysis

    This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
    2. Phosphoglycerate kinase 3, cytosolic (PGK3)
    3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
    4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
    5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g63250), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (At3g25960), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At5g08490), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei203SubstrateBy similarity1
    Binding sitei212SubstrateBy similarity1
    Active sitei255Proton donorBy similarity1
    Metal bindingi290MagnesiumBy similarity1
    Metal bindingi340MagnesiumBy similarity1
    Binding sitei340SubstrateBy similarity1
    Metal bindingi365MagnesiumBy similarity1
    Binding sitei365SubstrateBy similarity1
    Active sitei390Proton acceptorBy similarity1
    Binding sitei441SubstrateBy similarity1

    GO - Molecular functioni

    • magnesium ion binding Source: InterPro
    • phosphopyruvate hydratase activity Source: TAIR

    GO - Biological processi

    • glycolytic process Source: UniProtKB-UniPathway
    • response to cytokinin Source: TAIR
    • trichome morphogenesis Source: TAIR

    Keywordsi

    Molecular functionLyase
    Biological processGlycolysis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G74030-MONOMER
    ReactomeiR-ATH-70171 Glycolysis
    R-ATH-70263 Gluconeogenesis
    UniPathwayiUPA00109; UER00187

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enolase 1, chloroplastic (EC:4.2.1.11)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyase 1
    2-phosphoglycerate dehydratase 1
    Gene namesi
    Name:ENO1
    Ordered Locus Names:At1g74030
    ORF Names:F2P9.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G74030
    TAIRilocus:2031476 AT1G74030

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Distorted trichomes and reduced numbers of root hairs.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 41ChloroplastSequence analysisAdd BLAST41
    ChainiPRO_000039951042 – 477Enolase 1, chloroplasticAdd BLAST436

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei476PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9C9C4
    PRIDEiQ9C9C4

    PTM databases

    iPTMnetiQ9C9C4

    Expressioni

    Tissue specificityi

    Highly expressed in young roots, young siliques, and shoot apex. Lowly expressed in young leaves, stems and cotyledons.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9C9C4 baseline and differential
    GenevisibleiQ9C9C4 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi28960, 1 interactor
    IntActiQ9C9C4, 1 interactor
    STRINGi3702.AT1G74030.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C9C4
    SMRiQ9C9C4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni417 – 420Substrate bindingBy similarity4

    Sequence similaritiesi

    Belongs to the enolase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG2670 Eukaryota
    COG0148 LUCA
    HOGENOMiHOG000072174
    InParanoidiQ9C9C4
    KOiK01689
    OMAiEFMIIPV
    OrthoDBiEOG093608UK
    PhylomeDBiQ9C9C4

    Family and domain databases

    CDDicd03313 enolase, 1 hit
    Gene3Di3.20.20.120, 1 hit
    3.30.390.10, 1 hit
    HAMAPiMF_00318 Enolase, 1 hit
    InterProiView protein in InterPro
    IPR000941 Enolase
    IPR036849 Enolase-like_C_sf
    IPR029017 Enolase-like_N
    IPR034390 Enolase-like_superfamily
    IPR020810 Enolase_C
    IPR020809 Enolase_CS
    IPR020811 Enolase_N
    PANTHERiPTHR11902 PTHR11902, 1 hit
    PfamiView protein in Pfam
    PF00113 Enolase_C, 1 hit
    PF03952 Enolase_N, 1 hit
    PIRSFiPIRSF001400 Enolase, 1 hit
    PRINTSiPR00148 ENOLASE
    SFLDiSFLDG00178 enolase, 1 hit
    SFLDS00001 Enolase, 1 hit
    SMARTiView protein in SMART
    SM01192 Enolase_C, 1 hit
    SM01193 Enolase_N, 1 hit
    SUPFAMiSSF51604 SSF51604, 1 hit
    TIGRFAMsiTIGR01060 eno, 1 hit
    PROSITEiView protein in PROSITE
    PS00164 ENOLASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9C9C4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALTTKPHHL QRSFLSPSRV SGERYLESAP SCLRFRRSGV QCSVVAKECR
    60 70 80 90 100
    VKGVKARQII DSRGNPTVEV DLITDDLYRS AVPSGASTGI YEALELRDGD
    110 120 130 140 150
    KSVYGGKGVL QAIKNINELV APKLIGVDVR NQADVDALML ELDGTPNKSK
    160 170 180 190 200
    LGANAILGVS LSVCRAGAGA KGVPLYKHIQ ETSGTKELVM PVPAFNVING
    210 220 230 240 250
    GSHAGNSLAM QEFMILPVGA TSFSEAFQMG SEVYHTLKGI IKTKYGQDAC
    260 270 280 290 300
    NVGDEGGFAP NVQDNREGLV LLIDAIEKAG YTGKIKIGMD VAASEFFMKD
    310 320 330 340 350
    GRYDLNFKKQ PNDGAHVLSA ESLADLYREF IKDFPIVSIE DPFDQDDWSS
    360 370 380 390 400
    WASLQSSVDI QLVGDDLLVT NPKRIAEAIK KQSCNALLLK VNQIGTVTES
    410 420 430 440 450
    IQAALDSKAA GWGVMVSHRS GETEDNFIAD LSVGLASGQI KTGAPCRSER
    460 470
    LSKYNQLLRI EEELGNVRYA GEAFRSP
    Length:477
    Mass (Da):51,474
    Last modified:June 1, 2001 - v1
    Checksum:i450D57056C64E43E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC016662 Genomic DNA Translation: AAG52510.1
    CP002684 Genomic DNA Translation: AEE35539.1
    AY034978 mRNA Translation: AAK59483.1
    AY062968 mRNA Translation: AAL33814.1
    PIRiB96768
    RefSeqiNP_177543.1, NM_106062.4
    UniGeneiAt.18207

    Genome annotation databases

    EnsemblPlantsiAT1G74030.1; AT1G74030.1; AT1G74030
    GeneIDi843741
    GrameneiAT1G74030.1; AT1G74030.1; AT1G74030
    KEGGiath:AT1G74030

    Similar proteinsi

    Entry informationi

    Entry nameiENO1_ARATH
    AccessioniPrimary (citable) accession number: Q9C9C4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 1, 2001
    Last modified: April 25, 2018
    This is version 115 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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