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Protein

Enolase 1, chloroplastic

Gene

ENO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.1 Publication

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Kineticsi

  1. KM=0.082 mM for 2-PGA (at pH 8)1 Publication
  2. KM=0.149 mM for 2-PGA (at pH 7)1 Publication
  3. KM=0.180 mM for PEP (at pH 8)1 Publication
  4. KM=0.534 mM for PEP (at pH 7)1 Publication
  5. KM=1.281 mM for 3-PGA (at pH 8)1 Publication
  6. KM=0.390 mM for 3-PGA (at pH 7)1 Publication

    pH dependencei

    Optimum pH is 7.5-8.5 for the forward reaction (PEP formation) and 6.0-7.5 for the reverse reaction (2-PGA formation).1 Publication

    Pathwayi: glycolysis

    This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
    2. Phosphoglycerate kinase 3, cytosolic (PGK3)
    3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
    4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
    5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (At5g63680), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (At3g25960), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (At5g08570), Pyruvate kinase (At5g56350), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (At2g36580), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei203SubstrateBy similarity1
    Binding sitei212SubstrateBy similarity1
    Active sitei255Proton donorBy similarity1
    Metal bindingi290MagnesiumBy similarity1
    Metal bindingi340MagnesiumBy similarity1
    Binding sitei340SubstrateBy similarity1
    Metal bindingi365MagnesiumBy similarity1
    Binding sitei365SubstrateBy similarity1
    Active sitei390Proton acceptorBy similarity1
    Binding sitei441SubstrateBy similarity1

    GO - Molecular functioni

    • magnesium ion binding Source: InterPro
    • phosphopyruvate hydratase activity Source: TAIR

    GO - Biological processi

    • glycolytic process Source: UniProtKB-UniPathway
    • response to cytokinin Source: TAIR
    • trichome morphogenesis Source: TAIR

    Keywordsi

    Molecular functionLyase
    Biological processGlycolysis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G74030-MONOMER.
    ReactomeiR-ATH-70171. Glycolysis.
    R-ATH-70263. Gluconeogenesis.
    UniPathwayiUPA00109; UER00187.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enolase 1, chloroplastic (EC:4.2.1.11)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyase 1
    2-phosphoglycerate dehydratase 1
    Gene namesi
    Name:ENO1
    Ordered Locus Names:At1g74030
    ORF Names:F2P9.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G74030.
    TAIRilocus:2031476. AT1G74030.

    Subcellular locationi

    GO - Cellular componenti

    • chloroplast Source: TAIR
    • chloroplast stroma Source: TAIR
    • phosphopyruvate hydratase complex Source: InterPro

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Distorted trichomes and reduced numbers of root hairs.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 41ChloroplastSequence analysisAdd BLAST41
    ChainiPRO_000039951042 – 477Enolase 1, chloroplasticAdd BLAST436

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei476PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9C9C4.
    PRIDEiQ9C9C4.

    PTM databases

    iPTMnetiQ9C9C4.

    Expressioni

    Tissue specificityi

    Highly expressed in young roots, young siliques, and shoot apex. Lowly expressed in young leaves, stems and cotyledons.1 Publication

    Gene expression databases

    GenevisibleiQ9C9C4. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi28960. 1 interactor.
    IntActiQ9C9C4. 1 interactor.
    STRINGi3702.AT1G74030.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C9C4.
    SMRiQ9C9C4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni417 – 420Substrate bindingBy similarity4

    Sequence similaritiesi

    Belongs to the enolase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG2670. Eukaryota.
    COG0148. LUCA.
    HOGENOMiHOG000072174.
    InParanoidiQ9C9C4.
    KOiK01689.
    OMAiEFMIIPV.
    OrthoDBiEOG093608UK.
    PhylomeDBiQ9C9C4.

    Family and domain databases

    CDDicd03313. enolase. 1 hit.
    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00318. Enolase. 1 hit.
    InterProiView protein in InterPro
    IPR000941. Enolase.
    IPR034390. Enolase-like_superfamily.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N-like.
    PANTHERiPTHR11902. PTHR11902. 1 hit.
    PfamiView protein in Pfam
    PF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    PIRSFiPIRSF001400. Enolase. 1 hit.
    PRINTSiPR00148. ENOLASE.
    SFLDiSFLDG00178. enolase. 1 hit.
    SFLDS00001. Enolase. 1 hit.
    SMARTiView protein in SMART
    SM01192. Enolase_C. 1 hit.
    SM01193. Enolase_N. 1 hit.
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01060. eno. 1 hit.
    PROSITEiView protein in PROSITE
    PS00164. ENOLASE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9C9C4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALTTKPHHL QRSFLSPSRV SGERYLESAP SCLRFRRSGV QCSVVAKECR
    60 70 80 90 100
    VKGVKARQII DSRGNPTVEV DLITDDLYRS AVPSGASTGI YEALELRDGD
    110 120 130 140 150
    KSVYGGKGVL QAIKNINELV APKLIGVDVR NQADVDALML ELDGTPNKSK
    160 170 180 190 200
    LGANAILGVS LSVCRAGAGA KGVPLYKHIQ ETSGTKELVM PVPAFNVING
    210 220 230 240 250
    GSHAGNSLAM QEFMILPVGA TSFSEAFQMG SEVYHTLKGI IKTKYGQDAC
    260 270 280 290 300
    NVGDEGGFAP NVQDNREGLV LLIDAIEKAG YTGKIKIGMD VAASEFFMKD
    310 320 330 340 350
    GRYDLNFKKQ PNDGAHVLSA ESLADLYREF IKDFPIVSIE DPFDQDDWSS
    360 370 380 390 400
    WASLQSSVDI QLVGDDLLVT NPKRIAEAIK KQSCNALLLK VNQIGTVTES
    410 420 430 440 450
    IQAALDSKAA GWGVMVSHRS GETEDNFIAD LSVGLASGQI KTGAPCRSER
    460 470
    LSKYNQLLRI EEELGNVRYA GEAFRSP
    Length:477
    Mass (Da):51,474
    Last modified:June 1, 2001 - v1
    Checksum:i450D57056C64E43E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC016662 Genomic DNA. Translation: AAG52510.1.
    CP002684 Genomic DNA. Translation: AEE35539.1.
    AY034978 mRNA. Translation: AAK59483.1.
    AY062968 mRNA. Translation: AAL33814.1.
    PIRiB96768.
    RefSeqiNP_177543.1. NM_106062.4.
    UniGeneiAt.18207.

    Genome annotation databases

    EnsemblPlantsiAT1G74030.1; AT1G74030.1; AT1G74030.
    GeneIDi843741.
    GrameneiAT1G74030.1; AT1G74030.1; AT1G74030.
    KEGGiath:AT1G74030.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiENO1_ARATH
    AccessioniPrimary (citable) accession number: Q9C9C4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: June 1, 2001
    Last modified: June 7, 2017
    This is version 110 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families