Q9C9B0 (U89B1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 89B1
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT89B1
EC=2.4.1.91
Flavonol 7-O-glucosyltransferase UGT89B1

Gene names

Name:	UGT89B1
Ordered Locus Names:	At1g73880
ORF Names:	F2P9.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses quercetin 3-O-glucosyltransferase, 7-O-glucosyltransferase and 4'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Ref.5 Ref.6
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside. UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Sequence caution	The sequence AAG52529.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM12962.1 differs from that shown. Reason: Frameshift at position 8.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR quercetin 4'-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR quercetin 7-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	UDP-glycosyltransferase 89B1		PRO_0000409137
Regions
	Region	348 – 350	3	UDP-glucose binding By similarity
	Region	365 – 373	9	UDP-glucose binding By similarity
	Region	387 – 390	4	UDP-glucose binding By similarity
Sites
	Binding site	289	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C9B0 [UniParc]. Last modified May 31, 2011. Version 2. Checksum: 293587240C506F6A	FASTA	473	52,106
	10 20 30 40 50 60 MKVNEENNKP TKTHVLIFPF PAQGHMIPLL DFTHRLALRG GAALKITVLV TPKNLPFLSP 70 80 90 100 110 120 LLSAVVNIEP LILPFPSHPS IPSGVENVQD LPPSGFPLMI HALGNLHAPL ISWITSHPSP 130 140 150 160 170 180 PVAIVSDFFL GWTKNLGIPR FDFSPSAAIT CCILNTLWIE MPTKINEDDD NEILHFPKIP 190 200 210 220 230 240 NCPKYRFDQI SSLYRSYVHG DPAWEFIRDS FRDNVASWGL VVNSFTAMEG VYLEHLKREM 250 260 270 280 290 300 GHDRVWAVGP IIPLSGDNRG GPTSVSVDHV MSWLDAREDN HVVYVCFGSQ VVLTKEQTLA 310 320 330 340 350 360 LASGLEKSGV HFIWAVKEPV EKDSTRGNIL DGFDDRVAGR GLVIRGWAPQ VAVLRHRAVG 370 380 390 400 410 420 AFLTHCGWNS VVEAVVAGVL MLTWPMRADQ YTDASLVVDE LKVGVRACEG PDTVPDPDEL 430 440 450 460 470 ARVFADSVTG NQTERIKAVE LRKAALDAIQ ERGSSVNDLD GFIQHVVSLG LNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[5]	"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates." Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J. J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC016662 Genomic DNA. Translation: AAG52529.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE35520.1. AY092963 mRNA. Translation: AAM12962.1. Frameshift. BT006596 mRNA. Translation: AAP31940.1.
IPI	IPI00519286.
PIR	D96766.
RefSeq	NP_177529.2. NM_106048.3.
UniGene	At.43757.
3D structure databases
ProteinModelPortal	Q9C9B0.
SMR	Q9C9B0. Positions 12-468.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G73880.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	Q9C9B0.
PRIDE	Q9C9B0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G73880.1; AT1G73880.1; AT1G73880.
GeneID	843725.
KEGG	ath:AT1G73880.
Organism-specific databases
TAIR	At1g73880.
Phylogenomic databases
eggNOG	NOG265147.
HOGENOM	HOG000237565.
InParanoid	Q9C9B0.
OMA	PAQGHMI.
Gene expression databases
Genevestigator	Q9C9B0.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U89B1_ARATH

Accession

Primary (citable) accession number: Q9C9B0
Secondary accession number(s): Q8RWP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	May 31, 2011
Last modified:	April 3, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program