Reviewed, UniProtKB/Swiss-Prot Q9C971 (G3OX4_ARATH)

Names and origin

Protein names

Recommended name:
    Gibberellin 3-beta-dioxygenase 4
    EC=1.14.11.15
Alternative name(s):
    Gibberellin 3 beta-hydroxylase 4
    GA 3-oxidase 4
      Short name=AtGA3ox4
      Short name=AtGA3ox3

Gene names

Ordered Locus Names:	At1g80330
ORF Names:	F5I6.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts the inactive gibbberellin precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1.
Catalytic activity	Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2.
Cofactor	Ascorbate By similarity. Iron By similarity.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily.
biophysicochemical properties	Kinetic parameters: KM=0.85 µM for GA20 Vmax=52500 pmol/sec/mg enzyme

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gibberellin biosynthetic process Ref.2 Inferred from direct assay. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gibberellin 3-beta-dioxygenase activity Ref.2 Inferred from direct assay. Source: TAIR iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Gibberellin 3-beta-dioxygenase 4		PRO_0000067316
Sites
	Active site	294	1	Potential
	Metal binding	227	1	Iron By similarity
	Metal binding	229	1	Iron By similarity
	Metal binding	284	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C971-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9A0333A2112308C1	FASTA	355	39,153
	10 20 30 40 50 60 MPSLAEEICI GNLGSLQTLP ESFTWKLTAA DSLLRPSSAV SFDAVEESIP VIDLSNPDVT 70 80 90 100 110 120 TLIGDASKTW GAFQIANHGI SQKLLDDIES LSKTLFDMPS ERKLEAASSD KGVSGYGEPR 130 140 150 160 170 180 ISPFFEKKMW SEGFTIADDS YRNHFNTLWP HDHTKYCGII QEYVDEMEKL ASRLLYCILG 190 200 210 220 230 240 SLGVTVEDIE WAHKLEKSGS KVGRGAIRLN HYPVCPEPER AMGLAAHTDS TILTILHQSN 250 260 270 280 290 300 TGGLQVFREE SGWVTVEPAP GVLVVNIGDL FHILSNGKIP SVVHRAKVNH TRSRISIAYL 310 320 330 340 350 WGGPAGDVQI APISKLTGPA EPSLYRSITW KEYLQIKYEV FDKAMDAIRV VNPTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Purification and kinetic studies of recombinant gibberellin dioxygenases." Lester D.R., Phillips A., Hedden P., Andersson I. BMC Plant Biol. 5:19-19(2005) [PubMed: 16181493] [Abstract] Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC018848 Genomic DNA. Translation: AAG52440.1.
IPI	IPI00519408.
PIR	H96834.
RefSeq	NP_178149.1.
UniGene	At.33922
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	844373.
GenomeReviews	Gene locus AT1G80330 in contig CT485782_GR.
KEGG	ath:AT1G80330.
NMPDR	fig|3702.1.peg.7620.
Organism-specific databases
GeneFarm	5170. 494.
TAIR	At1g80330.
Phylogenomic databases
OMA	Q9C971. ISIAYLW.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G80330-MON.
BRENDA	1.14.11.15. 302.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
ProtoNet	Search...

Entry information

Entry name

G3OX4_ARATH

Accession

Primary (citable) accession number: Q9C971

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)