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Protein

Caffeoylshikimate esterase

Gene

CSE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Esterase involved in the biosynthesis of lignin. Hydrolyzes caffeoylshikimate into caffeate and shikimate. Together with 4-coumarate--CoA ligase (4CL), acts on an alternative reaction for the formation of caffeoyl-CoA and bypasses the second reaction of shikimate O-hydroxycinnamoyltransferase (HST). Accepts also 4-coumaroylshikimate as substrate, but with lower activity. According to PubMed:20345607 and PubMed:22915575, posseses monoacylglycerol O-acyltransferase, monoacylglycerol lipase and lysophospholipase activities in vitro. With the association of ACBP2, may promote the degradation of lysophosphatidylcholine and detoxify the peroxidized membrane in response to cadmium-induced oxidative stress. However these results require additional confirmation in vivo.4 Publications

Catalytic activityi

Caffeoylshikimate + H2O = caffeate + shikimate.1 Publication

Kineticsi

  1. KM=96.5 µM for caffeoylshikimate2 Publications
  2. KM=211 µM for 4-coumarylshikimate2 Publications
  3. KM=6.6 µM for lysophosphatidylcholine (at pH 8.0 and 33 degrees Celsius)2 Publications
  1. Vmax=9.3 pmol/sec/mg enzyme toward caffeoylshikimate2 Publications
  2. Vmax=0.66 pmol/sec/mg enzyme toward 4-coumarylshikimate2 Publications
  3. Vmax=0.030 µmol/min/mg enzyme toward lysophosphatidylcholine (at pH 8.0 and 33 degrees Celsius)2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471NucleophileBy similarity
Active sitei268 – 2681Charge relay systemBy similarity
Active sitei298 – 2981Charge relay systemBy similarity

GO - Molecular functioni

  • 2-acylglycerol O-acyltransferase activity Source: TAIR
  • caffeoyl-CoA: alcohol caffeoyl transferase activity Source: TAIR
  • hydrolase activity Source: TAIR
  • lysophospholipase activity Source: TAIR

GO - Biological processi

  • lignin biosynthetic process Source: UniProtKB-KW
  • lipid metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to hydrogen peroxide Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to zinc ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lignin biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-1482883. Acyl chain remodeling of DAG and TAG.
R-ATH-426048. Arachidonate production from DAG.

Protein family/group databases

ESTHERiarath-F14G24.3. Monoglyceridelipase_lysophospholip.
MEROPSiS33.A37.

Names & Taxonomyi

Protein namesi
Recommended name:
Caffeoylshikimate esterase (EC:3.1.1.-)
Alternative name(s):
Lysophospholipase 2
Short name:
LysoPL2
Gene namesi
Name:CSE
Ordered Locus Names:At1g52760
ORF Names:F14G24.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G52760.

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Colocalizes with ACBP2.

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced height and weight of senescent plants due to reduced lignin content.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Caffeoylshikimate esterasePRO_0000424295Add
BLAST

Proteomic databases

PaxDbiQ9C942.
PRIDEiQ9C942.

Expressioni

Tissue specificityi

Expressed in vasculature of roots and leaves, stems, flowers and siliques.2 Publications

Inductioni

By zinc and H2O2.1 Publication

Gene expression databases

GenevisibleiQ9C942. AT.

Interactioni

Subunit structurei

Interacts with ACBP2.1 Publication

Protein-protein interaction databases

BioGridi26934. 5 interactions.
STRINGi3702.AT1G52760.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C942.
SMRiQ9C942. Positions 51-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1455. Eukaryota.
COG2267. LUCA.
HOGENOMiHOG000003228.
InParanoidiQ9C942.
KOiK18368.
OMAiMKPSKAH.
PhylomeDBiQ9C942.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR022742. Hydrolase_4.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSEAESSAN SAPATPPPPP NFWGTMPEEE YYTSQGVRNS KSYFETPNGK
60 70 80 90 100
LFTQSFLPLD GEIKGTVYMS HGYGSDTSWM FQKICMSFSS WGYAVFAADL
110 120 130 140 150
LGHGRSDGIR CYMGDMEKVA ATSLAFFKHV RCSDPYKDLP AFLFGESMGG
160 170 180 190 200
LVTLLMYFQS EPETWTGLMF SAPLFVIPED MKPSKAHLFA YGLLFGLADT
210 220 230 240 250
WAAMPDNKMV GKAIKDPEKL KIIASNPQRY TGKPRVGTMR ELLRKTQYVQ
260 270 280 290 300
ENFGKVTIPV FTAHGTADGV TCPTSSKLLY EKASSADKTL KIYEGMYHSL
310 320 330
IQGEPDENAE IVLKDMREWI DEKVKKYGSK TA
Length:332
Mass (Da):36,975
Last modified:June 1, 2001 - v1
Checksum:i985297835CF58AE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC019018 Genomic DNA. Translation: AAG52273.1.
CP002684 Genomic DNA. Translation: AEE32849.1.
AY054577 mRNA. Translation: AAK96768.1.
BT008729 mRNA. Translation: AAP42742.1.
PIRiF96568.
RefSeqiNP_175685.1. NM_104154.3.
UniGeneiAt.24466.

Genome annotation databases

EnsemblPlantsiAT1G52760.1; AT1G52760.1; AT1G52760.
GeneIDi841709.
GrameneiAT1G52760.1; AT1G52760.1; AT1G52760.
KEGGiath:AT1G52760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC019018 Genomic DNA. Translation: AAG52273.1.
CP002684 Genomic DNA. Translation: AEE32849.1.
AY054577 mRNA. Translation: AAK96768.1.
BT008729 mRNA. Translation: AAP42742.1.
PIRiF96568.
RefSeqiNP_175685.1. NM_104154.3.
UniGeneiAt.24466.

3D structure databases

ProteinModelPortaliQ9C942.
SMRiQ9C942. Positions 51-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26934. 5 interactions.
STRINGi3702.AT1G52760.1.

Protein family/group databases

ESTHERiarath-F14G24.3. Monoglyceridelipase_lysophospholip.
MEROPSiS33.A37.

Proteomic databases

PaxDbiQ9C942.
PRIDEiQ9C942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G52760.1; AT1G52760.1; AT1G52760.
GeneIDi841709.
GrameneiAT1G52760.1; AT1G52760.1; AT1G52760.
KEGGiath:AT1G52760.

Organism-specific databases

TAIRiAT1G52760.

Phylogenomic databases

eggNOGiKOG1455. Eukaryota.
COG2267. LUCA.
HOGENOMiHOG000003228.
InParanoidiQ9C942.
KOiK18368.
OMAiMKPSKAH.
PhylomeDBiQ9C942.

Enzyme and pathway databases

ReactomeiR-ATH-1482883. Acyl chain remodeling of DAG and TAG.
R-ATH-426048. Arachidonate production from DAG.

Miscellaneous databases

PROiQ9C942.

Gene expression databases

GenevisibleiQ9C942. AT.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR022742. Hydrolase_4.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Acyl-CoA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis."
    Gao W., Li H.Y., Xiao S., Chye M.L.
    Plant J. 62:989-1003(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH ACBP2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  5. "Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis."
    Gao W., Li H.Y., Xiao S., Chye M.L.
    Plant Signal. Behav. 5:1025-1027(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A bifunctional enzyme that has both monoacylglycerol acyltransferase and acyl hydrolase activities."
    Vijayaraj P., Jashal C.B., Vijayakumar A., Rani S.H., Venkata Rao D.K., Rajasekharan R.
    Plant Physiol. 160:667-683(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCSE_ARATH
AccessioniPrimary (citable) accession number: Q9C942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutant plants exhibit increased sensitivity to zinc, cadmium and H2O2.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.