Reviewed, UniProtKB/Swiss-Prot Q9C927 (PPA5_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 5
    EC=3.1.3.2

Gene names

Name:	PAP5
Synonyms:	AT9
Ordered Locus Names:	At1g52940
ORF Names:	F14G24.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C927-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: Q9C927-2)

The sequence of this isoform differs from the canonical sequence as follows:
     221-251: NKYTPQNSWLQDEFKKVNRSETPWLIVLVHA → STSPLWYSIKRASAYIIILSSL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 13	13	Potential
	Chain	14 – 396	383	Purple acid phosphatase 5		PRO_0000372810
Regions
	Region	287 – 289	3	Substrate binding By similarity
Sites
	Active site	260	1	Proton donor By similarity
	Metal binding	125	1	Iron By similarity
	Metal binding	153	1	Iron By similarity
	Metal binding	153	1	Zinc By similarity
	Metal binding	156	1	Iron By similarity
	Metal binding	190	1	Zinc By similarity
	Metal binding	250	1	Zinc By similarity
	Metal binding	287	1	Zinc By similarity
	Metal binding	289	1	Iron By similarity
	Binding site	190	1	Substrate By similarity
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	360	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	221 – 251	31	NKYTP…VLVHA → STSPLWYSIKRASAYIIILS SL in isoform 2.		VSP_037189

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 52296BA227053E84	FASTA	396	45,420
	10 20 30 40 50 60 MSLETFPPPA GYNAPEQVHI TQGDHNGRGM IISWVTSLNE DGSNVVTYWI ASSDGSDNKS 70 80 90 100 110 120 VIATTSSYRY FDYTSGYLHH AIIKELEYKT KYFYELGTGR STRQFNLTPP KVGPDVPYTF 130 140 150 160 170 180 GVIGDLGQTY ASNQTLYNYM SNPKGQAVLF AGDLSYADDH PNHDQSKWDS YGRFVEPSAA 190 200 210 220 230 240 YQPWIWAAGN HEIDYAQSIG ETQPFKPYKN RYHVPYRASQ NKYTPQNSWL QDEFKKVNRS 250 260 270 280 290 300 ETPWLIVLVH APWYNSNNYH YMEGESMRVT FEPWFVENKV DIVFAGHVHA YERSERVSNI 310 320 330 340 350 360 QYNITDGMST PVKDQNAPVY ITIGDGGNIE GIANIFTDPQ PSYSAFREAS FGHALLEIKN 370 380 390 RTHAHYTWHR NKEDEAVIAD SIWLKNRYYL PEEETI
	Isoform 2. Checksum: 086ED8BE5ACD7A42	FASTA	387	44,148
	10 20 30 40 50 60 MSLETFPPPA GYNAPEQVHI TQGDHNGRGM IISWVTSLNE DGSNVVTYWI ASSDGSDNKS 70 80 90 100 110 120 VIATTSSYRY FDYTSGYLHH AIIKELEYKT KYFYELGTGR STRQFNLTPP KVGPDVPYTF 130 140 150 160 170 180 GVIGDLGQTY ASNQTLYNYM SNPKGQAVLF AGDLSYADDH PNHDQSKWDS YGRFVEPSAA 190 200 210 220 230 240 YQPWIWAAGN HEIDYAQSIG ETQPFKPYKN RYHVPYRASQ STSPLWYSIK RASAYIIILS 250 260 270 280 290 300 SLPWYNSNNY HYMEGESMRV TFEPWFVENK VDIVFAGHVH AYERSERVSN IQYNITDGMS 310 320 330 340 350 360 TPVKDQNAPV YITIGDGGNI EGIANIFTDP QPSYSAFREA SFGHALLEIK NRTHAHYTWH 370 380 RNKEDEAVIA DSIWLKNRYY LPEEETI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"Differential expression of Arabidopsis thaliana acid phosphatases in response to abiotic stresses." Lohrasebi T., Malboobi M.A., Samaeian A. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 125-242 (ISOFORM 2). Tissue: Root.
[4]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC019018 Genomic DNA. Translation: AAG52275.1. DQ459170 mRNA. Translation: ABE97169.1. DQ874389 mRNA. Translation: ABI49506.1.
IPI	IPI00539319. IPI00929936.
RefSeq	NP_564619.1.
UniGene	At.37498
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
SMR	Q9C927. Positions 5-389.
ModBase	Search...
Genome annotation databases
GeneID	841727.
GenomeReviews	Gene locus AT1G52940 in contig CT485782_GR.
KEGG	ath:AT1G52940.
NMPDR	fig|3702.1.peg.4786.
Organism-specific databases
TAIR	At1g52940.
Phylogenomic databases
eggNOG	KOG1378.
HOGENOM	HBG316723.
InParanoid	Q9C927.
PhylomeDB	Q9C927.
Gene expression databases
Genevestigator	Q9C927.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR008963. Purple_acid_Pase-like_N. IPR015914. Purple_acid_Pase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA5_ARATH

Accession

Primary (citable) accession number: Q9C927
Secondary accession number(s): Q09LH2, Q1KS91

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)