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Protein

Beta-glucosidase 22

Gene

BGLU22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-D-glucosidase active on scopolin >> esculin >> 4-MU-glucoside. No activity with DIMBOA-glucoside, pNP-glucoside, oNP-glucoside and sinigrin as substrates.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Enzyme regulationi

Activated upon binding to PBP1 or PBP2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55SubstrateBy similarity1
Binding sitei158SubstrateBy similarity1
Binding sitei203SubstrateBy similarity1
Active sitei204Proton donorBy similarity1
Binding sitei346SubstrateBy similarity1
Active sitei418NucleophileBy similarity1
Binding sitei468SubstrateBy similarity1

GO - Molecular functioni

  • beta-glucosidase activity Source: TAIR
  • scopolin beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cellular response to cold Source: TAIR
  • cellular response to salt stress Source: TAIR
  • glucosinolate catabolic process Source: GO_Central
  • response to hormone Source: GO_Central
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G66280-MONOMER.
BRENDAi3.2.1.21. 399.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 22 (EC:3.2.1.21)
Short name:
AtBGLU22
Gene namesi
Name:BGLU22
Ordered Locus Names:At1g66280
ORF Names:T27F4.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G66280.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000038958425 – 524Beta-glucosidase 22Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi223 ↔ 230By similarity
Glycosylationi494N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9C8Y9.
PRIDEiQ9C8Y9.

PTM databases

iPTMnetiQ9C8Y9.

Expressioni

Tissue specificityi

Expressed exclusively in roots.1 Publication

Inductioni

Up-regulated by salt, 2,4-D and methyl jasmonate. Down-regulated by cold and manitol.1 Publication

Gene expression databases

GenevisibleiQ9C8Y9. AT.

Interactioni

Subunit structurei

Component of the PYK10 complex, at least composed of PYK10/BGLU23, BGLU21, BGLU22, JAL22, JAL23, PBP1/JAL30, PBP2/JAL31, JAL32, JAL33, JAL34, JAL35, GLL22 and GLL23.1 Publication

Protein-protein interaction databases

BioGridi28166. 4 interactors.
IntActiQ9C8Y9. 3 interactors.
STRINGi3702.AT1G66280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C8Y9.
SMRiQ9C8Y9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni475 – 476Substrate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi521 – 524Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ9C8Y9.
KOiK01188.
OMAiSARIYSE.
OrthoDBiEOG093612GG.
PhylomeDBiQ9C8Y9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C8Y9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALQKFPLLG LLFLITIVVS STIAVDDPVC PTTSKLSRAS FPNGFVFGTA
60 70 80 90 100
TAAFQVEGAI NETCRGPALW DIFCKRNPER CSGHNADVAV DFFHRYKEDI
110 120 130 140 150
QLMKNLNTDA FRLSIAWSRI FPHGRKEKGV SQAGVKFYHD LIDELLKNGI
160 170 180 190 200
IPFVTVFHWD TPQDLEDEYG GFLSENIVKD FREYADYVFT EYGGKVKNWI
210 220 230 240 250
TFNEPWVFAH AGYDVGKKAP GRCSRYLKGC EDRDGRSGYE AYLVSHNLLN
260 270 280 290 300
AHAEAVEVFR QKVKGGKIGI AHSPAWFEPH DLKDSNDVPT VSRVLDFMLG
310 320 330 340 350
WHLDPTTFGD YPQIMKDLLG HRLPKFTSSQ KAKLKDSTDF VGLNYYTSTF
360 370 380 390 400
SNHNEKPDPS TPSWKQDSLV AWEPKNVDHS AIGSQPLTAA LPVYAKGFRS
410 420 430 440 450
LLKYIKDKYA NPEIMIMENG YGDKLKDKDS VEVGTADYNR KYYLQRHLLA
460 470 480 490 500
MNEAICIDKV RVTGYFVWSL LDNFEWQDGY NNRFGLYYVD FKNNLTRYEK
510 520
ESAKYYKDFL GQGVRPSALK KDEL
Length:524
Mass (Da):59,781
Last modified:June 1, 2001 - v1
Checksum:iB05574AECFC56C41
GO
Isoform 2 (identifier: Q9C8Y9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     456-456: C → W
     457-524: Missing.

Note: No experimental confirmation available.
Show »
Length:456
Mass (Da):51,669
Checksum:i88D47178B41A2E9E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti280H → R in BAH56964 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038458456C → W in isoform 2. 1 Publication1
Alternative sequenceiVSP_038459457 – 524Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020665 Genomic DNA. Translation: AAG52159.1.
CP002684 Genomic DNA. Translation: AEE34490.1.
AY074378 mRNA. Translation: AAL67074.1.
AK318849 mRNA. Translation: BAH56964.1.
PIRiH96687.
RefSeqiNP_176802.1. NM_105299.3. [Q9C8Y9-1]
UniGeneiAt.47576.
At.75574.

Genome annotation databases

EnsemblPlantsiAT1G66280.1; AT1G66280.1; AT1G66280. [Q9C8Y9-1]
GeneIDi842945.
GrameneiAT1G66280.1; AT1G66280.1; AT1G66280.
KEGGiath:AT1G66280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020665 Genomic DNA. Translation: AAG52159.1.
CP002684 Genomic DNA. Translation: AEE34490.1.
AY074378 mRNA. Translation: AAL67074.1.
AK318849 mRNA. Translation: BAH56964.1.
PIRiH96687.
RefSeqiNP_176802.1. NM_105299.3. [Q9C8Y9-1]
UniGeneiAt.47576.
At.75574.

3D structure databases

ProteinModelPortaliQ9C8Y9.
SMRiQ9C8Y9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28166. 4 interactors.
IntActiQ9C8Y9. 3 interactors.
STRINGi3702.AT1G66280.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

PTM databases

iPTMnetiQ9C8Y9.

Proteomic databases

PaxDbiQ9C8Y9.
PRIDEiQ9C8Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66280.1; AT1G66280.1; AT1G66280. [Q9C8Y9-1]
GeneIDi842945.
GrameneiAT1G66280.1; AT1G66280.1; AT1G66280.
KEGGiath:AT1G66280.

Organism-specific databases

TAIRiAT1G66280.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ9C8Y9.
KOiK01188.
OMAiSARIYSE.
OrthoDBiEOG093612GG.
PhylomeDBiQ9C8Y9.

Enzyme and pathway databases

BioCyciARA:AT1G66280-MONOMER.
BRENDAi3.2.1.21. 399.

Miscellaneous databases

PROiQ9C8Y9.

Gene expression databases

GenevisibleiQ9C8Y9. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL22_ARATH
AccessioniPrimary (citable) accession number: Q9C8Y9
Secondary accession number(s): C0Z2N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.