Q9C8M3 (GSTUS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U28
Short name=AtGSTU28
EC=2.5.1.18

Alternative name(s):
GST class-tau member 28

Gene names

Name:	GSTU28
Ordered Locus Names:	At1g53680
ORF Names:	F22G10.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Ref.4.
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.3. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.3. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Glutathione S-transferase U28		PRO_0000413573
Regions
	Domain	6 – 85	80	GST N-terminal
	Domain	91 – 217	127	GST C-terminal
	Region	16 – 17	2	Glutathione binding By similarity
	Region	42 – 43	2	Glutathione binding By similarity
	Region	56 – 57	2	Glutathione binding By similarity
	Region	69 – 70	2	Glutathione binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C8M3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 1EC8B43B38573E5D	FASTA	224	25,591
	10 20 30 40 50 60 MGKENSKVVV LDFWASPYAM RTKVALREKG VEFEVQEEDL WNKSELLLKS NPVHKKVPVL 70 80 90 100 110 120 IHNNTPISES LIQVQYIDET WTDAASFLPS DPQSRATARF WADYADKTIS FEGGRKIWGN 130 140 150 160 170 180 KKGEEQEKGK KEFLESLKVL EAELGDKSYF GGETFGYVDI TLVPFYSWFY ALEKCGDFSV 190 200 210 220 EAECPKIVAW GKRCVERNSV AATLPESEKV YQQVLKLRQI FGVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily." Dixon D.P., Hawkins T., Hussey P.J., Edwards R. J. Exp. Bot. 60:1207-1218(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC024260 Genomic DNA. Translation: AAG51968.1. CP002684 Genomic DNA. Translation: AEE32981.1.
IPI	IPI00531999.
PIR	A96577.
RefSeq	NP_175772.1. NM_104246.1.
UniGene	At.52184.
3D structure databases
HSSP	HSSP built from PDB template 1OYJ based on UniProtKB O65032.
ProteinModelPortal	Q9C8M3.
SMR	Q9C8M3. Positions 7-224.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G53680.1-P.
Proteomic databases
PaxDb	Q9C8M3.
PRIDE	Q9C8M3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G53680.1; AT1G53680.1; AT1G53680.
GeneID	841805.
KEGG	ath:AT1G53680.
Organism-specific databases
TAIR	At1g53680.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125749.
InParanoid	Q9C8M3.
KO	K00799.
OMA	CVERNSV.
PhylomeDB	Q9C8M3.
ProtClustDB	CLSN2914524.
Gene expression databases
Genevestigator	Q9C8M3.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTUS_ARATH

Accession

Primary (citable) accession number: Q9C8M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program