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Protein

ABC transporter C family member 1

Gene

ABCC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pump for glutathione S-conjugates. Mediates the transport of S-(2,4-dinitrophenyl)-glutathione (DNP-GS), GSSG, cyanidin 3-glucoside-GS (C3G-GS) and metolachlor-GS (MOC-GS).1 Publication

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Kineticsi

  1. KM=70 µM for DNP-GS (at pH 8)1 Publication
  2. KM=220 µM for GSSG (at pH 8)1 Publication
  3. KM=60 µM for MOC-GS (at pH 8)1 Publication
  1. Vmax=0.82 nmol/min/mg enzyme with DNP-GS as substrate (at pH 8)1 Publication
  2. Vmax=0.68 nmol/min/mg enzyme with GSSG as substrate (at pH 8)1 Publication
  3. Vmax=1.75 nmol/min/mg enzyme with MOC-GS as substrate (at pH 8)1 Publication
  4. Vmax=0.79 nmol/min/mg enzyme with C3G-GS as substrate (at pH 8)1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi649 – 656ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1271 – 1278ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • (+)-abscisic acid D-glucopyranosyl ester transmembrane transporter activity Source: TAIR
  • ATPase activity, coupled to transmembrane movement of substances Source: TAIR
  • ATPase-coupled arsenite transmembrane transporter activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • phytochelatin transmembrane transporter activity Source: TAIR
  • xenobiotic-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • (+)-abscisic acid D-glucopyranosyl ester transmembrane transport Source: TAIR
  • arsenite transport Source: TAIR
  • response to arsenic-containing substance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G30400-MONOMER.
ReactomeiR-ATH-2142850. Hyaluronan biosynthesis and export.
R-ATH-382556. ABC-family proteins mediated transport.

Protein family/group databases

TCDBi3.A.1.208.20. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter C family member 1 (EC:3.6.3.44)
Short name:
ABC transporter ABCC.1
Short name:
AtABCC1
Alternative name(s):
ATP-energized glutathione S-conjugate pump 1
Glutathione S-conjugate-transporting ATPase 1
Multidrug resistance-associated protein 1
Gene namesi
Name:ABCC1
Synonyms:EST1, MRP1
Ordered Locus Names:At1g30400
ORF Names:T4K22.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G30400.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei37 – 57HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei73 – 93HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei110 – 130HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei145 – 165HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei174 – 194HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei336 – 356HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei440 – 460HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei527 – 547HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei557 – 577HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei909 – 929HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei951 – 971HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1027 – 1049HelicalPROSITE-ProRule annotationAdd BLAST23
Transmembranei1053 – 1072HelicalPROSITE-ProRule annotationAdd BLAST20
Transmembranei1138 – 1158HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1172 – 1192HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plant-type vacuole Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002260721 – 1622ABC transporter C family member 1Add BLAST1622

Proteomic databases

PaxDbiQ9C8G9.
PRIDEiQ9C8G9.

PTM databases

iPTMnetiQ9C8G9.

Expressioni

Tissue specificityi

Ubiquitous, with higher levels in leaves and stems and lower levels in roots. Localized in the root apex, root hair tips and root epidermis.4 Publications

Inductioni

Slightly induced by benoxacor, cloquintocet, fenchlorazol and fluorazol.1 Publication

Gene expression databases

GenevisibleiQ9C8G9. AT.

Interactioni

Subunit structurei

Interacts with calmodulin (CaM), PAS1 and FKBP42/TWD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FKBP42Q9LDC04EBI-637633,EBI-360006

Protein-protein interaction databases

BioGridi25155. 1 interactor.
IntActiQ9C8G9. 2 interactors.
STRINGi3702.AT1G30400.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C8G9.
SMRiQ9C8G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini302 – 582ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST281
Domaini614 – 838ABC transporter 1PROSITE-ProRule annotationAdd BLAST225
Domaini916 – 1200ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST285
Domaini1237 – 1471ABC transporter 2PROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1231 – 1246Interaction with calmodulin and FKP42/TWD1Add BLAST16

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
InParanoidiQ9C8G9.
OMAiVVETKTY.
OrthoDBiEOG093600AW.
PhylomeDBiQ9C8G9.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C8G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFEPLDWYC KPVPNGVWTK TVDYAFGAYT PCAIDSFVLG ISHLVLLILC
60 70 80 90 100
LYRLWLITKD HKVDKFCLRS KWFSYFLALL AAYATAEPLF RLVMRISVLD
110 120 130 140 150
LDGAGFPPYE AFMLVLEAFA WGSALVMTVV ETKTYIHELR WYVRFAVIYA
160 170 180 190 200
LVGDMVLLNL VLSVKEYYGS FKLYLYISEV AVQVAFGTLL FVYFPNLDPY
210 220 230 240 250
PGYTPVGTEN SEDYEYEELP GGENICPERH ANLFDSIFFS WLNPLMTLGS
260 270 280 290 300
KRPLTEKDVW HLDTWDKTET LMRSFQKSWD KELEKPKPWL LRALNNSLGG
310 320 330 340 350
RFWWGGFWKI GNDCSQFVGP LLLNELLKSM QLNEPAWIGY IYAISIFVGV
360 370 380 390 400
VLGVLCEAQY FQNVMRVGYR LRSALIAAVF RKSLRLTNEG RKKFQTGKIT
410 420 430 440 450
NLMTTDAESL QQICQSLHTM WSAPFRIIVA LVLLYQQLGV ASIIGALFLV
460 470 480 490 500
LMFPIQTVII SKTQKLTKEG LQRTDKRIGL MNEVLAAMDT VKCYAWENSF
510 520 530 540 550
QSKVQTVRDD ELSWFRKAQL LSAFNMFILN SIPVLVTVVS FGVFSLLGGD
560 570 580 590 600
LTPARAFTSL SLFSVLRFPL FMLPNIITQM VNANVSLNRL EEVLSTEERV
610 620 630 640 650
LLPNPPIEPG QPAISIRNGY FSWDSKADRP TLSNINLDIP LGSLVAVVGS
660 670 680 690 700
TGEGKTSLIS AMLGELPARS DATVTLRGSV AYVPQVSWIF NATVRDNILF
710 720 730 740 750
GAPFDQEKYE RVIDVTALQH DLELLPGGDL TEIGERGVNI SGGQKQRVSM
760 770 780 790 800
ARAVYSNSDV CILDDPLSAL DAHVGQQVFE KCIKRELGQT TRVLVTNQLH
810 820 830 840 850
FLSQVDKILL VHEGTVKEEG TYEELCHSGP LFQRLMENAG KVEDYSEENG
860 870 880 890 900
EAEVDQTSVK PVENGNANNL QKDGIETKNS KEGNSVLVKR EERETGVVSW
910 920 930 940 950
KVLERYQNAL GGAWVVMMLV ICYVLTQVFR VSSSTWLSEW TDSGTPKTHG
960 970 980 990 1000
PLFYNIVYAL LSFGQVSVTL INSYWLIMSS LYAAKKMHDA MLGSILRAPM
1010 1020 1030 1040 1050
VFFQTNPLGR IINRFAKDMG DIDRTVAVFV NMFMGSIAQL LSTVILIGIV
1060 1070 1080 1090 1100
STLSLWAIMP LLVVFYGAYL YYQNTSREIK RMDSTTRSPV YAQFGEALNG
1110 1120 1130 1140 1150
LSSIRAYKAY DRMAEINGRS MDNNIRFTLV NMAANRWLGI RLEVLGGLMV
1160 1170 1180 1190 1200
WLTASLAVMQ NGKAANQQAY ASTMGLLLSY ALSITSSLTA VLRLASLAEN
1210 1220 1230 1240 1250
SLNSVERVGN YIEIPSEAPL VIENNRPPPG WPSSGSIKFE DVVLRYRPEL
1260 1270 1280 1290 1300
PPVLHGVSFL ISPMDKVGIV GRTGAGKSSL LNALFRIVEL EKGRILIDEC
1310 1320 1330 1340 1350
DIGRFGLMDL RKVLGIIPQA PVLFSGTVRF NLDPFSEHND ADLWESLERA
1360 1370 1380 1390 1400
HLKDTIRRNP LGLDAEVTEA GENFSVGQRQ LLSLARALLR RSKILVLDEA
1410 1420 1430 1440 1450
TAAVDVRTDV LIQKTIREEF KSCTMLIIAH RLNTIIDCDK VLVLDSGKVQ
1460 1470 1480 1490 1500
EFSSPENLLS NGESSFSKMV QSTGTANAEY LRSITLENKR TREANGDDSQ
1510 1520 1530 1540 1550
PLEGQRKWQA SSRWAAAAQF ALAVSLTSSH NDLQSLEIED DNSILKKTKD
1560 1570 1580 1590 1600
AVVTLRSVLE GKHDKEIEDS LNQSDISRER WWPSLYKMVE GLAVMSRLAR
1610 1620
NRMQHPDYNL EGKSFDWDNV EM
Length:1,622
Mass (Da):181,927
Last modified:June 1, 2001 - v1
Checksum:iB71E085798F1BBD6
GO

Sequence cautioni

The sequence AAL90919 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti765D → E in AAB67319 (PubMed:9223346).Curated1
Sequence conflicti765D → E in AAB71832 (PubMed:9223346).Curated1
Sequence conflicti833Q → P in AAB67319 (PubMed:9223346).Curated1
Sequence conflicti833Q → P in AAB71832 (PubMed:9223346).Curated1
Sequence conflicti855D → H in AAB67319 (PubMed:9223346).Curated1
Sequence conflicti855D → H in AAB71832 (PubMed:9223346).Curated1
Sequence conflicti934S → I in AAB67319 (PubMed:9223346).Curated1
Sequence conflicti934S → I in AAB71832 (PubMed:9223346).Curated1
Sequence conflicti1152L → W in AAB67319 (PubMed:9223346).Curated1
Sequence conflicti1152L → W in AAB71832 (PubMed:9223346).Curated1
Sequence conflicti1314L → V in AAB67319 (PubMed:9223346).Curated1
Sequence conflicti1314L → V in AAB71832 (PubMed:9223346).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008124 mRNA. Translation: AAB67319.1.
AF008125 Genomic DNA. Translation: AAB71832.1.
AC025295 Genomic DNA. Translation: AAG51096.1.
CP002684 Genomic DNA. Translation: AEE31212.1.
CP002684 Genomic DNA. Translation: AEE31213.1.
AY090258 mRNA. Translation: AAL90919.1. Different initiation.
U96398 mRNA. Translation: AAC49796.1.
PIRiD86428.
RefSeqiNP_001031116.1. NM_001036039.2.
NP_174329.1. NM_102777.3.
UniGeneiAt.21491.

Genome annotation databases

EnsemblPlantsiAT1G30400.1; AT1G30400.1; AT1G30400.
AT1G30400.2; AT1G30400.2; AT1G30400.
GeneIDi839920.
GrameneiAT1G30400.1; AT1G30400.1; AT1G30400.
AT1G30400.2; AT1G30400.2; AT1G30400.
KEGGiath:AT1G30400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008124 mRNA. Translation: AAB67319.1.
AF008125 Genomic DNA. Translation: AAB71832.1.
AC025295 Genomic DNA. Translation: AAG51096.1.
CP002684 Genomic DNA. Translation: AEE31212.1.
CP002684 Genomic DNA. Translation: AEE31213.1.
AY090258 mRNA. Translation: AAL90919.1. Different initiation.
U96398 mRNA. Translation: AAC49796.1.
PIRiD86428.
RefSeqiNP_001031116.1. NM_001036039.2.
NP_174329.1. NM_102777.3.
UniGeneiAt.21491.

3D structure databases

ProteinModelPortaliQ9C8G9.
SMRiQ9C8G9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25155. 1 interactor.
IntActiQ9C8G9. 2 interactors.
STRINGi3702.AT1G30400.1.

Protein family/group databases

TCDBi3.A.1.208.20. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiQ9C8G9.

Proteomic databases

PaxDbiQ9C8G9.
PRIDEiQ9C8G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G30400.1; AT1G30400.1; AT1G30400.
AT1G30400.2; AT1G30400.2; AT1G30400.
GeneIDi839920.
GrameneiAT1G30400.1; AT1G30400.1; AT1G30400.
AT1G30400.2; AT1G30400.2; AT1G30400.
KEGGiath:AT1G30400.

Organism-specific databases

TAIRiAT1G30400.

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
InParanoidiQ9C8G9.
OMAiVVETKTY.
OrthoDBiEOG093600AW.
PhylomeDBiQ9C8G9.

Enzyme and pathway databases

BioCyciARA:AT1G30400-MONOMER.
ReactomeiR-ATH-2142850. Hyaluronan biosynthesis and export.
R-ATH-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiQ9C8G9.

Gene expression databases

GenevisibleiQ9C8G9. AT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAB1C_ARATH
AccessioniPrimary (citable) accession number: Q9C8G9
Secondary accession number(s): O24635, Q8RX76, Q9SAQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.