ABC transporter C family member 1 - Arabidopsis thaliana (Mouse-ear cress)

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Q9C8G9 (AB1C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
ABC transporter C family member 1
Short name=ABC transporter ABCC.1
Short name=AtABCC1
EC=3.6.3.44

Alternative name(s):
ATP-energized glutathione S-conjugate pump 1
Glutathione S-conjugate-transporting ATPase 1
Multidrug resistance-associated protein 1

Gene names

Name:	ABCC1
Synonyms:	EST1, MRP1
Ordered Locus Names:	At1g30400
ORF Names:	T4K22.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1622 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pump for glutathione S-conjugates. Mediates the transport of S-(2,4-dinitrophenyl)-glutathione (DNP-GS), GSSG, cyanidin 3-glucoside-GS (C3G-GS) and metolachlor-GS (MOC-GS). Ref.1
Catalytic activity	ATP + H₂O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subunit structure	Interacts with calmodulin (CaM), PAS1 and FKBP42/TWD1. Ref.11
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast. Ref.11 Ref.12 Ref.13
Tissue specificity	Ubiquitous, with higher levels in leaves and stems and lower levels in roots. Localized in the root apex, root hair tips and root epidermis. Ref.1 Ref.7 Ref.10 Ref.11
Induction	Slightly induced by benoxacor, cloquintocet, fenchlorazol and fluorazol. Ref.6
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Biophysicochemical properties	Kinetic parameters: K_M=70 µM for DNP-GS (at pH 8) Ref.7 K_M=220 µM for GSSG (at pH 8) K_M=60 µM for MOC-GS (at pH 8) V_max=0.82 nmol/min/mg enzyme with DNP-GS as substrate (at pH 8) V_max=0.68 nmol/min/mg enzyme with GSSG as substrate (at pH 8) V_max=1.75 nmol/min/mg enzyme with MOC-GS as substrate (at pH 8) V_max=0.79 nmol/min/mg enzyme with C3G-GS as substrate (at pH 8)
Sequence caution	The sequence AAL90919.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to arsenic-containing substance Inferred from mutant phenotype. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arsenite-transmembrane transporting ATPase activity Inferred from direct assay. Source: TAIR calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW phytochelatin transmembrane transporter activity Inferred from direct assay. Source: TAIR xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FKBP42	Q9LDC0	4	EBI-637633,EBI-360006

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1622

1622

ABC transporter C family member 1

PRO_0000226072

Regions

Transmembrane

37 – 57

Helical; Potential

Transmembrane

73 – 93

Helical; Potential

Transmembrane

110 – 130

Helical; Potential

Transmembrane

145 – 165

Helical; Potential

Transmembrane

174 – 194

Helical; Potential

Transmembrane

336 – 356

Helical; Potential

Transmembrane

440 – 460

Helical; Potential

Transmembrane

527 – 547

Helical; Potential

Transmembrane

557 – 577

Helical; Potential

Transmembrane

909 – 929

Helical; Potential

Transmembrane

951 – 971

Helical; Potential

Transmembrane

1027 – 1049

Helical; Potential

Transmembrane

1053 – 1072

Helical; Potential

Transmembrane

1138 – 1158

Helical; Potential

Transmembrane

1172 – 1192

Helical; Potential

Domain

302 – 582

281

ABC transmembrane type-1 1

Domain

614 – 838

225

ABC transporter 1

Domain

916 – 1200

285

ABC transmembrane type-1 2

Domain

1237 – 1471

235

ABC transporter 2

Nucleotide binding

649 – 656

ATP 1 Potential

Nucleotide binding

1271 – 1278

ATP 2 Potential

Region

1231 – 1246

Interaction with calmodulin and FKP42/TWD1

Experimental info

Sequence conflict

765

D → E in AAB67319. Ref.1

Sequence conflict

765

D → E in AAB71832. Ref.1

Sequence conflict

833

Q → P in AAB67319. Ref.1

Sequence conflict

833

Q → P in AAB71832. Ref.1

Sequence conflict

855

D → H in AAB67319. Ref.1

Sequence conflict

855

D → H in AAB71832. Ref.1

Sequence conflict

934

S → I in AAB67319. Ref.1

Sequence conflict

934

S → I in AAB71832. Ref.1

Sequence conflict

1152

L → W in AAB67319. Ref.1

Sequence conflict

1152

L → W in AAB71832. Ref.1

Sequence conflict

1314

L → V in AAB67319. Ref.1

Sequence conflict

1314

L → V in AAB71832. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9C8G9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: B71E085798F1BBD6
Show »

FASTA

1,622

181,927

        10         20         30         40         50         60 
MGFEPLDWYC KPVPNGVWTK TVDYAFGAYT PCAIDSFVLG ISHLVLLILC LYRLWLITKD 

        70         80         90        100        110        120 
HKVDKFCLRS KWFSYFLALL AAYATAEPLF RLVMRISVLD LDGAGFPPYE AFMLVLEAFA 

       130        140        150        160        170        180 
WGSALVMTVV ETKTYIHELR WYVRFAVIYA LVGDMVLLNL VLSVKEYYGS FKLYLYISEV 

       190        200        210        220        230        240 
AVQVAFGTLL FVYFPNLDPY PGYTPVGTEN SEDYEYEELP GGENICPERH ANLFDSIFFS 

       250        260        270        280        290        300 
WLNPLMTLGS KRPLTEKDVW HLDTWDKTET LMRSFQKSWD KELEKPKPWL LRALNNSLGG 

       310        320        330        340        350        360 
RFWWGGFWKI GNDCSQFVGP LLLNELLKSM QLNEPAWIGY IYAISIFVGV VLGVLCEAQY 

       370        380        390        400        410        420 
FQNVMRVGYR LRSALIAAVF RKSLRLTNEG RKKFQTGKIT NLMTTDAESL QQICQSLHTM 

       430        440        450        460        470        480 
WSAPFRIIVA LVLLYQQLGV ASIIGALFLV LMFPIQTVII SKTQKLTKEG LQRTDKRIGL 

       490        500        510        520        530        540 
MNEVLAAMDT VKCYAWENSF QSKVQTVRDD ELSWFRKAQL LSAFNMFILN SIPVLVTVVS 

       550        560        570        580        590        600 
FGVFSLLGGD LTPARAFTSL SLFSVLRFPL FMLPNIITQM VNANVSLNRL EEVLSTEERV 

       610        620        630        640        650        660 
LLPNPPIEPG QPAISIRNGY FSWDSKADRP TLSNINLDIP LGSLVAVVGS TGEGKTSLIS 

       670        680        690        700        710        720 
AMLGELPARS DATVTLRGSV AYVPQVSWIF NATVRDNILF GAPFDQEKYE RVIDVTALQH 

       730        740        750        760        770        780 
DLELLPGGDL TEIGERGVNI SGGQKQRVSM ARAVYSNSDV CILDDPLSAL DAHVGQQVFE 

       790        800        810        820        830        840 
KCIKRELGQT TRVLVTNQLH FLSQVDKILL VHEGTVKEEG TYEELCHSGP LFQRLMENAG 

       850        860        870        880        890        900 
KVEDYSEENG EAEVDQTSVK PVENGNANNL QKDGIETKNS KEGNSVLVKR EERETGVVSW 

       910        920        930        940        950        960 
KVLERYQNAL GGAWVVMMLV ICYVLTQVFR VSSSTWLSEW TDSGTPKTHG PLFYNIVYAL 

       970        980        990       1000       1010       1020 
LSFGQVSVTL INSYWLIMSS LYAAKKMHDA MLGSILRAPM VFFQTNPLGR IINRFAKDMG 

      1030       1040       1050       1060       1070       1080 
DIDRTVAVFV NMFMGSIAQL LSTVILIGIV STLSLWAIMP LLVVFYGAYL YYQNTSREIK 

      1090       1100       1110       1120       1130       1140 
RMDSTTRSPV YAQFGEALNG LSSIRAYKAY DRMAEINGRS MDNNIRFTLV NMAANRWLGI 

      1150       1160       1170       1180       1190       1200 
RLEVLGGLMV WLTASLAVMQ NGKAANQQAY ASTMGLLLSY ALSITSSLTA VLRLASLAEN 

      1210       1220       1230       1240       1250       1260 
SLNSVERVGN YIEIPSEAPL VIENNRPPPG WPSSGSIKFE DVVLRYRPEL PPVLHGVSFL 

      1270       1280       1290       1300       1310       1320 
ISPMDKVGIV GRTGAGKSSL LNALFRIVEL EKGRILIDEC DIGRFGLMDL RKVLGIIPQA 

      1330       1340       1350       1360       1370       1380 
PVLFSGTVRF NLDPFSEHND ADLWESLERA HLKDTIRRNP LGLDAEVTEA GENFSVGQRQ 

      1390       1400       1410       1420       1430       1440 
LLSLARALLR RSKILVLDEA TAAVDVRTDV LIQKTIREEF KSCTMLIIAH RLNTIIDCDK 

      1450       1460       1470       1480       1490       1500 
VLVLDSGKVQ EFSSPENLLS NGESSFSKMV QSTGTANAEY LRSITLENKR TREANGDDSQ 

      1510       1520       1530       1540       1550       1560 
PLEGQRKWQA SSRWAAAAQF ALAVSLTSSH NDLQSLEIED DNSILKKTKD AVVTLRSVLE 

      1570       1580       1590       1600       1610       1620 
GKHDKEIEDS LNQSDISRER WWPSLYKMVE GLAVMSRLAR NRMQHPDYNL EGKSFDWDNV 


EM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene." Lu Y.-P., Li Z.-S., Rea P.A. Proc. Natl. Acad. Sci. U.S.A. 94:8243-8248(1997) [PubMed: 9223346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 820-1622. Strain: cv. Columbia.
[5]	"Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics." Tommasini R., Vogt E., Schmid J., Fromentau M., Amrhein N., Martinoia E. FEBS Lett. 411:206-210(1997) [PubMed: 9271206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1390-1486.
[6]	"Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana." Sanchez-Fernandez R., Ardiles-Diaz W., Van Montagu M., Inze D., May M.J. Mol. Gen. Genet. 258:655-662(1998) [PubMed: 9671034] [Abstract] Cited for: INDUCTION.
[7]	"AtMRP2, an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with AtMRP1." Lu Y.-P., Li Z.-S., Drozdowicz Y.M., Hoertensteiner S., Martinoia E., Rea P.A. Plant Cell 10:267-282(1998) [PubMed: 9490749] [Abstract] Cited for: TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
[8]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"Multifunctionality of plant ABC transporters -- more than just detoxifiers." Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B. Planta 214:345-355(2002) [PubMed: 11855639] [Abstract] Cited for: GENE FAMILY.
[10]	"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana." Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B. Planta 216:107-119(2002) [PubMed: 12430019] [Abstract] Cited for: TISSUE SPECIFICITY.
[11]	"Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters." Geisler M., Girin M., Brandt S., Vincenzetti V., Plaza S., Paris N., Kobae Y., Maeshima M., Billion K., Kolukisaoglu U.H., Schulz B., Martinoia E. Mol. Biol. Cell 15:3393-3405(2004) [PubMed: 15133126] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH PAS1; CALMODULIN AND FKBP42/TWD1.
[12]	"Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana." Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T. Plant Cell Physiol. 45:672-683(2004) [PubMed: 15215502] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Large-scale characterization of integral proteins from Arabidopsis vacuolar membrane by two-dimensional liquid chromatography." Szponarski W., Sommerer N., Boyer J.-C., Rossignol M., Gibrat R. Proteomics 4:397-406(2004) [PubMed: 14760709] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008124 mRNA. Translation: AAB67319.1. AF008125 Genomic DNA. Translation: AAB71832.1. AC025295 Genomic DNA. Translation: AAG51096.1. CP002684 Genomic DNA. Translation: AEE31212.1. CP002684 Genomic DNA. Translation: AEE31213.1. AY090258 mRNA. Translation: AAL90919.1. Different initiation. U96398 mRNA. Translation: AAC49796.1.
IPI	IPI00519163.
PIR	D86428.
RefSeq	NP_001031116.1. NM_001036039.1. NP_174329.1. NM_102777.2.
UniGene	At.21491.
3D structure databases
ProteinModelPortal	Q9C8G9.
SMR	Q9C8G9. Positions 291-840, 900-1473.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C8G9. 2 interactions.
STRING	Q9C8G9.
Protein family/group databases
TCDB	3.A.1.208.21. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	Q9C8G9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G30400.1; AT1G30400.1; AT1G30400. AT1G30400.2; AT1G30400.2; AT1G30400.
GeneID	839920.
GenomeReviews	Gene locus AT1G30400 in contig CT485782_GR.
KEGG	ath:AT1G30400.
NMPDR	fig\|3702.1.peg.3375.
Organism-specific databases
TAIR	At1g30400.
Phylogenomic databases
eggNOG	KOG0054.
GeneTree	EPGT00070000028299.
HOGENOM	HBG758042.
InParanoid	Q9C8G9.
OMA	VLATKNR.
PhylomeDB	Q9C8G9.
ProtClustDB	PLN03130.
Gene expression databases
Genevestigator	Q9C8G9.
GermOnline	AT1G30400. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB1C_ARATH

Accession

Primary (citable) accession number: Q9C8G9
Secondary accession number(s): O24635, Q8RX76, Q9SAQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents