Q9C8G4 (PLY4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase 4
EC=4.2.2.2

Gene names

Ordered Locus Names:	At1g30350
ORF Names:	T4K22.5

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 368	343	Probable pectate lyase 4		PRO_0000024868
Sites
	Active site	247	1	Potential
	Metal binding	167	1	Calcium By similarity
	Metal binding	191	1	Calcium By similarity
	Metal binding	195	1	Calcium By similarity
Amino acid modifications
	Glycosylation	27	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9C8G4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F35EDEDB2CED34BC	FASTA	368	40,576
	10 20 30 40 50 60 MASLVVIVSL LLAAFASPLL ETAHSYNVTA PRVSLNPIDA CWRRNPKWAT NRQALAHCAV 70 80 90 100 110 120 GYGKAAIGGK NGPIYVVTNP SDNPTRPSPG TLRYAVSQPK PLWITFARDM VIVLKSQLMI 130 140 150 160 170 180 NSYKTIDGRG AKVEIANGPC LRIRQVKHVI IHGISIHDCK ADPNGMDGDG IRVFQSTHVW 190 200 210 220 230 240 IDHCFLSRCH DGLIDVIVSS TAVTISNNYF TQHDKVMLLG HDDSYMGDKD MRVTIAFNTF 250 260 270 280 290 300 GPGLIERMPR VRRGYAHVAN NRYEKWQMYA IGGSANPIIF SEGNYFVAPE KRSSKQVTKR 310 320 330 340 350 360 MMAGPDSKRW KWGTSRDVFM NGAFFGPPGV IVRPLYKGGE GFRVAHGSLV PSLTSSAGPL RCYVGRIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC025295 Genomic DNA. Translation: AAG51103.1. CP002684 Genomic DNA. Translation: AEE31208.1. DQ446309 mRNA. Translation: ABE65673.1.
IPI	IPI00543154.
PIR	G86427.
RefSeq	NP_174324.1. NM_102772.2.
UniGene	At.51837.
3D structure databases
ProteinModelPortal	Q9C8G4.
SMR	Q9C8G4. Positions 35-361.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C8G4.
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q9C8G4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G30350.1; AT1G30350.1; AT1G30350.
GeneID	839915.
KEGG	ath:AT1G30350.
NMPDR	fig|3702.1.peg.3371.
Organism-specific databases
TAIR	At1g30350.
Phylogenomic databases
GeneTree	EPGT00050000008990.
InParanoid	Q9C8G4.
OMA	TIAFNTF.
PhylomeDB	Q9C8G4.
ProtClustDB	CLSN2914504.
Gene expression databases
ArrayExpress	Q9C8G4.
Genevestigator	Q9C8G4.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY4_ARATH

Accession

Primary (citable) accession number: Q9C8G4
Secondary accession number(s): Q1PFQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program