Reviewed, UniProtKB/Swiss-Prot Q9C888 (PLDE1_ARATH)

Names and origin

Protein names

Recommended name:
    Phospholipase D epsilon
      Short name=AtPLDepsilon
      Short name=PLD epsilon
    EC=3.1.4.4
Alternative name(s):
    PLDalpha3

Gene names

Name:	PLDEPSILON
Ordered Locus Names:	At1g55180
ORF Names:	F7A10.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium By similarity.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from mutant phenotype. Source: TAIR cell growth Inferred from mutant phenotype. Source: TAIR cellular response to nitrogen starvation Inferred from mutant phenotype. Source: TAIR cellular response to phosphate starvation Inferred from mutant phenotype. Source: TAIR cellular response to potassium ion starvation Inferred from mutant phenotype. Source: TAIR phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro phospholipid catabolic process Inferred from direct assay. Source: TAIR positive regulation of nitrogen utilization Inferred from mutant phenotype. Source: TAIR post-embryonic development Inferred from mutant phenotype. Source: TAIR response to osmotic stress Inferred from mutant phenotype. Source: TAIR root development Inferred from mutant phenotype. Source: TAIR
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase D activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	Phospholipase D epsilon		PRO_0000218816
Regions
	Domain	13 – 109	97	C2
	Domain	301 – 339	39	PLD phosphodiesterase 1
	Domain	610 – 637	28	PLD phosphodiesterase 2
Sites
	Active site	306	1	Potential
	Active site	308	1	Potential
	Active site	313	1	Potential
	Active site	615	1	Potential
	Active site	617	1	Potential
	Active site	622	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9C888-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5E9DA51B463994CC	FASTA	762	86,770
	10 20 30 40 50 60 MELEEQKKYF HGTLEITIFD ATPFSPPFPF NCICTKPKAA YVTIKINKKK VAKTSSEYDR 70 80 90 100 110 120 IWNQTFQILC AHPVTDTTIT ITLKTRCSVL GRFRISAEQI LTSNSAVING FFPLIADNGS 130 140 150 160 170 180 TKRNLKLKCL MWFRPAYLEP GWCRALEEAS FQGIRNASFP QRSNCRVVLY QDAHHKATFD 190 200 210 220 230 240 PRVDDVPFNA RNLWEDVYKA IESARHLVYI AGWALNPNLV LVRDNETEIP HAVGVTVGEL 250 260 270 280 290 300 LKRKSEEGVA VRVMLWNDET SLPMIKNKGV MRTNVERALA YFRNTNVVCR LCPRLHKKLP 310 320 330 340 350 360 TAFAHHQKTI TLDTRVTNSS TKEREIMSFL GGFDLCDGRY DTEEHSLFRT LGTEADFYQT 370 380 390 400 410 420 SVAGAKLSRG GPREPWHDCH VSVVGGAAWD VLKNFEQRWT KQCNPSVLVN TSGIRNLVNL 430 440 450 460 470 480 TGPTEENNRK WNVQVLRSID HISATEMPRG LPVEKSVHDG YVAAIRKAER FIYIENQYFM 490 500 510 520 530 540 GSCDHWESKN DKICSGCTNL IPVEIALKIA AKIRARERFA VYIVIPMWPE GPPESETVEE 550 560 570 580 590 600 ILHWTRETMS MMYQIIGEAI WEVGDKSHPR DYLNFFCLAN REEKRDGEFE AVSSPHQKTH 610 620 630 640 650 660 YWNAQRNRRF MVYVHSKLMI VDDTYILIGS ANINQRSMDG CRDTEIAIGC YQTNTNNTNE 670 680 690 700 710 720 IQAYRLSLWY EHTGGKITAD DLSSSEPESL ECVRGLRTIG EQMWEIYSGD KVVDMLGIHL 730 740 750 760 VAYPISVTGD GAVEEVGDGC FPDTKTLVKG KRSKMFPPVL TT

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC027034 Genomic DNA. Translation: AAG51567.1.
IPI	IPI00525972.
PIR	E96593.
RefSeq	NP_175914.1.
UniGene	At.37104
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C888.
Genome annotation databases
GeneID	841961.
GenomeReviews	Gene locus AT1G55180 in contig CT485782_GR.
KEGG	ath:AT1G55180.
NMPDR	fig|3702.1.peg.5040.
Organism-specific databases
TAIR	At1g55180.
Enzyme and pathway databases
BRENDA	3.1.4.4. 302.
Gene expression databases
ArrayExpress	Q9C888.
Genevestigator	Q9C888.
GermOnline	AT1G55180. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50004. C2. False negative. PS50035. PLD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDE1_ARATH

Accession

Primary (citable) accession number: Q9C888

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	June 1, 2001
Last modified:	October 13, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)