Reviewed, UniProtKB/Swiss-Prot Q9C826 (ABA2_ARATH)

Names and origin

Protein names

Recommended name:
    Xanthoxin dehydrogenase
    EC=1.1.1.288
Alternative name(s):
    Xanthoxin oxidase
    Short-chain alcohol dehydrogenase ABA2
    Protein GLUCOSE INSENSITIVE 1
    Protein IMPAIRED SUCROSE INDUCTION 4
    Protein SALOBRENO 3
    Protein SUGAR INSENSITIVE 4
    Protein SALT RESISTANT 1

Gene names

Name:	ABA2
Synonyms:	GIN1, ISI4, SAN3, SDR1, SIS4, SRE1
Ordered Locus Names:	At1g52340
ORF Names:	F19K6.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of abscisic acid. Ref.3 Ref.4 Ref.7
Catalytic activity	Xanthoxin + NAD+ = abscisic aldehyde + NADH.
Subcellular location	Cytoplasm. Ref.3
Tissue specificity	Predominantly in roots and stems, and at lower levels in leaves and seeds. Ref.3 Ref.7
Induction	Constitutively expressed and not up regulated by osmotic stress. Ref.3 Ref.4 Ref.7
Miscellaneous	The biological substrate is probably 2-cis,4-trans-xanthoxin.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
biophysicochemical properties	Kinetic parameters: no activity with xanthoxic acid, ethanol, isopropanol, butanol, cyclohexanol and 2,6-dimethylcyclohexanol. KM=19 µM for xanthoxin KM=10 mM for 3,5,5'-trimethylcyclohexanol

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Ref.4 Inferred from mutant phenotype. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from mutant phenotype. Source: TAIR regulation of abscisic acid biosynthetic process Inferred from mutant phenotype. Source: TAIR response to heat Inferred from mutant phenotype. Source: TAIR response to water deprivation Inferred from mutant phenotype. Source: TAIR sugar mediated signaling Traceable author statement. Source: TAIR
Cellular component	cytosol Ref.3 Inferred from direct assay. Source: TAIR
Molecular function	binding Inferred from electronic annotation. Source: InterPro xanthoxin dehydrogenase activity Ref.7 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Xanthoxin dehydrogenase		PRO_0000282347
Experimental info
	Mutagenesis	28	1	G → R in aba2-12/sre1-2; reduced absicic acid synthesis. Ref.7
	Mutagenesis	45	1	A → V in aba2-4/sis4-2; reduced absicic acid synthesis. Ref.3
	Mutagenesis	145	1	R → C in gin1-2; reduced absicic acid synthesis. Ref.3
	Mutagenesis	162	1	G → R in aba2-3/sis4-1; reduced absicic acid synthesis. Ref.3
	Mutagenesis	176	1	S → F in aba2-13/san3-1; reduced absicic acid synthesis. Ref.7
	Mutagenesis	236	1	A → V in isi4; reduced absicic acid synthesis. Ref.6
	Mutagenesis	264	1	S → N in aba2-1; reduced absicic acid synthesis. Ref.3 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9C826-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8B555DAED7386DF4	FASTA	285	30,272
	10 20 30 40 50 60 MSTNTESSSY SSLPSQRLLG KVALITGGAT GIGESIVRLF HKHGAKVCIV DLQDDLGGEV 70 80 90 100 110 120 CKSLLRGESK ETAFFIHGDV RVEDDISNAV DFAVKNFGTL DILINNAGLC GAPCPDIRNY 130 140 150 160 170 180 SLSEFEMTFD VNVKGAFLSM KHAARVMIPE KKGSIVSLCS VGGVVGGVGP HSYVGSKHAV 190 200 210 220 230 240 LGLTRSVAAE LGQHGIRVNC VSPYAVATKL ALAHLPEEER TEDAFVGFRN FAAANANLKG 250 260 270 280 VELTVDDVAN AVLFLASDDS RYISGDNLMI DGGFTCTNHS FKVFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions." Cheng W.-H., Endo A., Zhou L., Penney J., Chen H.-C., Arroyo A., Leon P., Nambara E., Asami T., Seo M., Koshiba T., Sheen J. Plant Cell 14:2723-2743(2002) [PubMed: 12417697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF ALA-45; ARG-145; GLY-162 AND SER-264, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, FUNCTION. Strain: cv. Columbia.
[4]	"Biochemical characterization of the aba2 and aba3 mutants in Arabidopsis thaliana." Schwartz S.H., Leon-Kloosterziel K.M., Koornneef M., Zeevaart J.A. Plant Physiol. 114:161-166(1997) [PubMed: 9159947] [Abstract] Cited for: FUNCTION, INDUCTION.
[5]	"Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant." Zhou L., Jang J.-C., Jones T.L., Sheen J. Proc. Natl. Acad. Sci. U.S.A. 95:10294-10299(1998) [PubMed: 9707641] [Abstract] Cited for: IDENTIFICATION.
[6]	"Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling." Rook F., Corke F., Card R., Munz G., Smith C., Bevan M.W. Plant J. 26:421-433(2001) [PubMed: 11439129] [Abstract] Cited for: IDENTIFICATION, MUTAGENESIS OF ALA-236. Strain: cv. Columbia.
[7]	"The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde." Gonzalez-Guzman M., Apostolova N., Belles J.M., Barrero J.M., Piqueras P., Ponce M.R., Micol J.L., Serrano R., Rodriguez P.L. Plant Cell 14:1833-1846(2002) [PubMed: 12172025] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-28; SER-176 AND SER-264. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC037424 Genomic DNA. Translation: AAG51536.1. BT003412 mRNA. Translation: AAO30075.1. AY099603 mRNA. Translation: AAM20454.1. AY082344 mRNA. Translation: AAL99237.1. AY082345 Genomic DNA. Translation: AAL99238.1.
IPI	IPI00534478.
PIR	F96563.
RefSeq	NP_175644.1.
UniGene	At.37618
3D structure databases
HSSP	HSSP built from PDB template 1DFI based on UniProtKB P29132.
ModBase	Search...
Proteomic databases
PRIDE	Q9C826.
Genome annotation databases
GeneID	841665.
GenomeReviews	Gene locus AT1G52340 in contig CT485782_GR.
KEGG	ath:AT1G52340.
NMPDR	fig|3702.1.peg.4716.
Organism-specific databases
TAIR	At1g52340.
Phylogenomic databases
OMA	Q9C826. HGAKVCI.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G52340-MON.
BRENDA	1.1.1.288. 302.
Gene expression databases
ArrayExpress	Q9C826.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABA2_ARATH

Accession

Primary (citable) accession number: Q9C826

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)