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Q9C826 (ABA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xanthoxin dehydrogenase
EC=1.1.1.288
Alternative name(s):
Protein GLUCOSE INSENSITIVE 1
Protein IMPAIRED SUCROSE INDUCTION 4
Protein SALOBRENO 3
Protein SALT RESISTANT 1
Protein SUGAR INSENSITIVE 4
Short-chain alcohol dehydrogenase ABA2
Xanthoxin oxidase
Gene names
Name:ABA2
Synonyms:GIN1, ISI4, SAN3, SDR1, SIS4, SRE1
Ordered Locus Names:At1g52340
ORF Names:F19K6.3
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of abscisic acid. Ref.4 Ref.5 Ref.8

Catalytic activity

Xanthoxin + NAD+ = abscisic aldehyde + NADH.

Subcellular location

Cytoplasm Ref.4.

Tissue specificity

Predominantly in roots and stems, and at lower levels in leaves and seeds. Ref.4 Ref.8

Induction

Constitutively expressed and not up regulated by osmotic stress. Ref.4 Ref.5 Ref.8

Miscellaneous

The biological substrate is probably 2-cis,4-trans-xanthoxin.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Biophysicochemical properties

Kinetic parameters:

no activity with xanthoxic acid, ethanol, isopropanol, butanol, cyclohexanol and 2,6-dimethylcyclohexanol.

KM=19 µM for xanthoxin Ref.8

KM=10 mM for 3,5,5'-trimethylcyclohexanol

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Xanthoxin dehydrogenase
PRO_0000282347

Experimental info

Mutagenesis281G → R in aba2-12/sre1-2; reduced absicic acid synthesis. Ref.8
Mutagenesis451A → V in aba2-4/sis4-2; reduced absicic acid synthesis. Ref.4
Mutagenesis1451R → C in gin1-2; reduced absicic acid synthesis. Ref.4
Mutagenesis1621G → R in aba2-3/sis4-1; reduced absicic acid synthesis. Ref.4
Mutagenesis1761S → F in aba2-13/san3-1; reduced absicic acid synthesis. Ref.8
Mutagenesis2361A → V in isi4; reduced absicic acid synthesis. Ref.7
Mutagenesis2641S → N in aba2-1; reduced absicic acid synthesis. Ref.4 Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q9C826 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 8B555DAED7386DF4

FASTA28530,272
        10         20         30         40         50         60 
MSTNTESSSY SSLPSQRLLG KVALITGGAT GIGESIVRLF HKHGAKVCIV DLQDDLGGEV 

        70         80         90        100        110        120 
CKSLLRGESK ETAFFIHGDV RVEDDISNAV DFAVKNFGTL DILINNAGLC GAPCPDIRNY 

       130        140        150        160        170        180 
SLSEFEMTFD VNVKGAFLSM KHAARVMIPE KKGSIVSLCS VGGVVGGVGP HSYVGSKHAV 

       190        200        210        220        230        240 
LGLTRSVAAE LGQHGIRVNC VSPYAVATKL ALAHLPEEER TEDAFVGFRN FAAANANLKG 

       250        260        270        280 
VELTVDDVAN AVLFLASDDS RYISGDNLMI DGGFTCTNHS FKVFR

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions."
Cheng W.-H., Endo A., Zhou L., Penney J., Chen H.-C., Arroyo A., Leon P., Nambara E., Asami T., Seo M., Koshiba T., Sheen J.
Plant Cell 14:2723-2743(2002) [PubMed: 12417697] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF ALA-45; ARG-145; GLY-162 AND SER-264, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, FUNCTION.
Strain: cv. Columbia.
[5]"Biochemical characterization of the aba2 and aba3 mutants in Arabidopsis thaliana."
Schwartz S.H., Leon-Kloosterziel K.M., Koornneef M., Zeevaart J.A.
Plant Physiol. 114:161-166(1997) [PubMed: 9159947] [Abstract]
Cited for: FUNCTION, INDUCTION.
[6]"Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant."
Zhou L., Jang J.-C., Jones T.L., Sheen J.
Proc. Natl. Acad. Sci. U.S.A. 95:10294-10299(1998) [PubMed: 9707641] [Abstract]
Cited for: IDENTIFICATION.
[7]"Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling."
Rook F., Corke F., Card R., Munz G., Smith C., Bevan M.W.
Plant J. 26:421-433(2001) [PubMed: 11439129] [Abstract]
Cited for: IDENTIFICATION, MUTAGENESIS OF ALA-236.
Strain: cv. Columbia.
[8]"The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde."
Gonzalez-Guzman M., Apostolova N., Belles J.M., Barrero J.M., Piqueras P., Ponce M.R., Micol J.L., Serrano R., Rodriguez P.L.
Plant Cell 14:1833-1846(2002) [PubMed: 12172025] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-28; SER-176 AND SER-264.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC037424 Genomic DNA. Translation: AAG51536.1.
CP002684 Genomic DNA. Translation: AEE32789.1.
BT003412 mRNA. Translation: AAO30075.1.
AY099603 mRNA. Translation: AAM20454.1.
AY082344 mRNA. Translation: AAL99237.1.
AY082345 Genomic DNA. Translation: AAL99238.1.
IPIIPI00534478.
PIRF96563.
RefSeqNP_175644.1. NM_104113.4.
UniGeneAt.37618.
At.66802.

3D structure databases

HSSPHSSP built from PDB template 1NFF based on UniProtKB P69167.
ProteinModelPortalQ9C826.
SMRQ9C826. Positions 15-283.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9C826. 4 interactions.
STRINGQ9C826.

Proteomic databases

PRIDEQ9C826.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G52340.1; AT1G52340.1; AT1G52340.
GeneID841665.
GenomeReviewsGene locus AT1G52340 in contig CT485782_GR.
KEGGath:AT1G52340.
NMPDRfig|3702.1.peg.4716.

Organism-specific databases

TAIRAt1g52340.

Phylogenomic databases

eggNOGKOG0725.
GeneTreeEPGT00070000027921.
HOGENOMHBG750976.
InParanoidQ9C826.
OMAISGDNLM.
PhylomeDBQ9C826.
ProtClustDBPLN02253.

Enzyme and pathway databases

BioCycARA:AT1G52340-MONOMER.
MetaCyc:AT1G52340-MONOMER.

Gene expression databases

ArrayExpressQ9C826.
GenevestigatorQ9C826.

Family and domain databases

InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK09841.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABA2_ARATH
AccessionPrimary (citable) accession number: Q9C826
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2001
Last modified: December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents