Reviewed,
UniProtKB/Swiss-Prot Q9C826 (ABA2_ARATH)
Last modified
June 16, 2009.
Version 50.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Xanthoxin dehydrogenase EC=1.1.1.288 Alternative name(s): Xanthoxin oxidase Short-chain alcohol dehydrogenase ABA2 Protein GLUCOSE INSENSITIVE 1 Protein IMPAIRED SUCROSE INDUCTION 4 Protein SALOBRENO 3 Protein SUGAR INSENSITIVE 4 Protein SALT RESISTANT 1 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 285 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the biosynthesis of abscisic acid. Ref.3 Ref.4 Ref.7 |
| Catalytic activity | Xanthoxin + NAD+ = abscisic aldehyde + NADH. |
| Subcellular location | |
| Tissue specificity | Predominantly in roots and stems, and at lower levels in leaves and seeds. Ref.3 Ref.7 |
| Induction | Constitutively expressed and not up regulated by osmotic stress. Ref.3 Ref.4 Ref.7 |
| Miscellaneous | The biological substrate is probably 2-cis,4-trans-xanthoxin. |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| biophysicochemical properties | Kinetic parameters: no activity with xanthoxic acid, ethanol, isopropanol, butanol, cyclohexanol and 2,6-dimethylcyclohexanol. KM=19 µM for xanthoxin KM=10 mM for 3,5,5'-trimethylcyclohexanol |
Ontologies
| Keywords | |
|---|---|
| Biological process | Abscisic acid biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | abscisic acid biosynthetic process Ref.4 Inferred from mutant phenotype. Source: TAIR oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic processInferred from mutant phenotype. Source: TAIR regulation of abscisic acid biosynthetic processInferred from mutant phenotype. Source: TAIR response to heatInferred from mutant phenotype. Source: TAIR response to water deprivationInferred from mutant phenotype. Source: TAIR sugar mediated signalingTraceable author statement. Source: TAIR |
| Cellular component | cytosol Ref.3 Inferred from direct assay. Source: TAIR |
| Molecular function | binding Inferred from electronic annotation. Source: InterPro xanthoxin dehydrogenase activity Ref.7Inferred from direct assay. Source: TAIR |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 285 | 285 | Xanthoxin dehydrogenase | PRO_0000282347 | |||||
Experimental info | |||||||||
| Mutagenesis | 28 | 1 | G → R in aba2-12/sre1-2; reduced absicic acid synthesis. Ref.7 | ||||||
| Mutagenesis | 45 | 1 | A → V in aba2-4/sis4-2; reduced absicic acid synthesis. Ref.3 | ||||||
| Mutagenesis | 145 | 1 | R → C in gin1-2; reduced absicic acid synthesis. Ref.3 | ||||||
| Mutagenesis | 162 | 1 | G → R in aba2-3/sis4-1; reduced absicic acid synthesis. Ref.3 | ||||||
| Mutagenesis | 176 | 1 | S → F in aba2-13/san3-1; reduced absicic acid synthesis. Ref.7 | ||||||
| Mutagenesis | 236 | 1 | A → V in isi4; reduced absicic acid synthesis. Ref.6 | ||||||
| Mutagenesis | 264 | 1 | S → N in aba2-1; reduced absicic acid synthesis. Ref.3 Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [3] | "A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions." Cheng W.-H., Endo A., Zhou L., Penney J., Chen H.-C., Arroyo A., Leon P., Nambara E., Asami T., Seo M., Koshiba T., Sheen J. Plant Cell 14:2723-2743(2002) [PubMed: 12417697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF ALA-45; ARG-145; GLY-162 AND SER-264, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, FUNCTION. Strain: cv. Columbia. |
| [4] | "Biochemical characterization of the aba2 and aba3 mutants in Arabidopsis thaliana." Schwartz S.H., Leon-Kloosterziel K.M., Koornneef M., Zeevaart J.A. Plant Physiol. 114:161-166(1997) [PubMed: 9159947] [Abstract] Cited for: FUNCTION, INDUCTION. |
| [5] | "Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant." Zhou L., Jang J.-C., Jones T.L., Sheen J. Proc. Natl. Acad. Sci. U.S.A. 95:10294-10299(1998) [PubMed: 9707641] [Abstract] Cited for: IDENTIFICATION. |
| [6] | "Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling." Rook F., Corke F., Card R., Munz G., Smith C., Bevan M.W. Plant J. 26:421-433(2001) [PubMed: 11439129] [Abstract] Cited for: IDENTIFICATION, MUTAGENESIS OF ALA-236. Strain: cv. Columbia. |
| [7] | "The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde." Gonzalez-Guzman M., Apostolova N., Belles J.M., Barrero J.M., Piqueras P., Ponce M.R., Micol J.L., Serrano R., Rodriguez P.L. Plant Cell 14:1833-1846(2002) [PubMed: 12172025] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-28; SER-176 AND SER-264. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| AC037424 Genomic DNA. Translation: AAG51536.1. BT003412 mRNA. Translation: AAO30075.1. AY099603 mRNA. Translation: AAM20454.1. AY082344 mRNA. Translation: AAL99237.1. AY082345 Genomic DNA. Translation: AAL99238.1. | |
| IPI | IPI00534478. |
| PIR | F96563. |
| RefSeq | NP_175644.1. |
| UniGene | At.37618 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DFI based on UniProtKB P29132. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q9C826. |
Genome annotation databases | |
| GeneID | 841665. |
| GenomeReviews | Gene locus AT1G52340 in contig CT485782_GR. |
| KEGG | ath:AT1G52340. |
| NMPDR | fig|3702.1.peg.4716. |
Organism-specific databases | |
| TAIR | At1g52340. |
Phylogenomic databases | |
| OMA | Q9C826. HGAKVCI. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT1G52340-MON. |
| BRENDA | 1.1.1.288. 302. |
Gene expression databases | |
| ArrayExpress | Q9C826. |
Family and domain databases | |
| InterPro | IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR19410. ADH_short_C2. 1 hit. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. PR00080. SDRFAMILY. |
| PROSITE | PS00061. ADH_SHORT. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ABA2_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9C826 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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