Q9C826 (ABA2_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 74.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Xanthoxin dehydrogenase EC=1.1.1.288 Alternative name(s): Protein GLUCOSE INSENSITIVE 1 Protein IMPAIRED SUCROSE INDUCTION 4 Protein SALOBRENO 3 Protein SALT RESISTANT 1 Protein SUGAR INSENSITIVE 4 Short-chain alcohol dehydrogenase ABA2 Xanthoxin oxidase | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 285 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in the biosynthesis of abscisic acid. Ref.4 Ref.5 Ref.8 |
| Catalytic activity | Xanthoxin + NAD+ = abscisic aldehyde + NADH. |
| Subcellular location | |
| Tissue specificity | Predominantly in roots and stems, and at lower levels in leaves and seeds. Ref.4 Ref.8 |
| Induction | Constitutively expressed and not up regulated by osmotic stress. Ref.4 Ref.5 Ref.8 |
| Miscellaneous | The biological substrate is probably 2-cis,4-trans-xanthoxin. |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| Biophysicochemical properties | Kinetic parameters: no activity with xanthoxic acid, ethanol, isopropanol, butanol, cyclohexanol and 2,6-dimethylcyclohexanol. KM=19 µM for xanthoxin Ref.8 KM=10 mM for 3,5,5'-trimethylcyclohexanol |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 285 | 285 | Xanthoxin dehydrogenase | PRO_0000282347 | |||||
Experimental info | |||||||||
| Mutagenesis | 28 | 1 | G → R in aba2-12/sre1-2; reduced absicic acid synthesis. Ref.8 | ||||||
| Mutagenesis | 45 | 1 | A → V in aba2-4/sis4-2; reduced absicic acid synthesis. Ref.4 | ||||||
| Mutagenesis | 145 | 1 | R → C in gin1-2; reduced absicic acid synthesis. Ref.4 | ||||||
| Mutagenesis | 162 | 1 | G → R in aba2-3/sis4-1; reduced absicic acid synthesis. Ref.4 | ||||||
| Mutagenesis | 176 | 1 | S → F in aba2-13/san3-1; reduced absicic acid synthesis. Ref.8 | ||||||
| Mutagenesis | 236 | 1 | A → V in isi4; reduced absicic acid synthesis. Ref.7 | ||||||
| Mutagenesis | 264 | 1 | S → N in aba2-1; reduced absicic acid synthesis. Ref.4 Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions." Cheng W.-H., Endo A., Zhou L., Penney J., Chen H.-C., Arroyo A., Leon P., Nambara E., Asami T., Seo M., Koshiba T., Sheen J. Plant Cell 14:2723-2743(2002) [PubMed: 12417697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF ALA-45; ARG-145; GLY-162 AND SER-264, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, FUNCTION. Strain: cv. Columbia. |
| [5] | "Biochemical characterization of the aba2 and aba3 mutants in Arabidopsis thaliana." Schwartz S.H., Leon-Kloosterziel K.M., Koornneef M., Zeevaart J.A. Plant Physiol. 114:161-166(1997) [PubMed: 9159947] [Abstract] Cited for: FUNCTION, INDUCTION. |
| [6] | "Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant." Zhou L., Jang J.-C., Jones T.L., Sheen J. Proc. Natl. Acad. Sci. U.S.A. 95:10294-10299(1998) [PubMed: 9707641] [Abstract] Cited for: IDENTIFICATION. |
| [7] | "Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling." Rook F., Corke F., Card R., Munz G., Smith C., Bevan M.W. Plant J. 26:421-433(2001) [PubMed: 11439129] [Abstract] Cited for: IDENTIFICATION, MUTAGENESIS OF ALA-236. Strain: cv. Columbia. |
| [8] | "The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde." Gonzalez-Guzman M., Apostolova N., Belles J.M., Barrero J.M., Piqueras P., Ponce M.R., Micol J.L., Serrano R., Rodriguez P.L. Plant Cell 14:1833-1846(2002) [PubMed: 12172025] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-28; SER-176 AND SER-264. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AC037424 Genomic DNA. Translation: AAG51536.1. CP002684 Genomic DNA. Translation: AEE32789.1. BT003412 mRNA. Translation: AAO30075.1. AY099603 mRNA. Translation: AAM20454.1. AY082344 mRNA. Translation: AAL99237.1. AY082345 Genomic DNA. Translation: AAL99238.1. |
| IPI | IPI00534478. |
| PIR | F96563. |
| RefSeq | NP_175644.1. NM_104113.4. |
| UniGene | At.37618. At.66802. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NFF based on UniProtKB P69167. |
| ProteinModelPortal | Q9C826. |
| SMR | Q9C826. Positions 15-283. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9C826. 4 interactions. |
| STRING | Q9C826. |
Proteomic databases | |
| PRIDE | Q9C826. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT1G52340.1; AT1G52340.1; AT1G52340. |
| GeneID | 841665. |
| GenomeReviews | Gene locus AT1G52340 in contig CT485782_GR. |
| KEGG | ath:AT1G52340. |
| NMPDR | fig|3702.1.peg.4716. |
Organism-specific databases | |
| TAIR | At1g52340. |
Phylogenomic databases | |
| eggNOG | KOG0725. |
| GeneTree | EPGT00070000027921. |
| HOGENOM | HBG750976. |
| InParanoid | Q9C826. |
| OMA | ISGDNLM. |
| PhylomeDB | Q9C826. |
| ProtClustDB | PLN02253. |
Enzyme and pathway databases | |
| BioCyc | ARA:AT1G52340-MONOMER. MetaCyc:AT1G52340-MONOMER. |
Gene expression databases | |
| ArrayExpress | Q9C826. |
| Genevestigator | Q9C826. |
Family and domain databases | |
| InterPro | IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K09841. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. PR00080. SDRFAMILY. |
| PROSITE | PS00061. ADH_SHORT. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ABA2_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9C826 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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