Q9C809 (B3GT8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,3-galactosyltransferase 8
EC=2.4.1.-

Gene names

Name:	B3GALT8
Ordered Locus Names:	At1g33430
ORF Names:	F10C21.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence BX813522 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9C809-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Probable beta-1,3-galactosyltransferase 8		PRO_0000359418
Regions
	Transmembrane	5 – 27	23	Helical; Signal-anchor for type II membrane protein; Potential
Amino acid modifications
	Glycosylation	117	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7C289A6881DAB366	FASTA	395	44,732
	10 20 30 40 50 60 MRAKAASGKA IIVLCLASFL AGSLFMSRTL SRSYIPEEED HHLTKHLSKH LEIQKDCDEH 70 80 90 100 110 120 KRKLIESKSR DIIGEVSRTH QAVKSLERTM STLEMELAAA RTSDRSSEFW SERSAKNQSR 130 140 150 160 170 180 LQKVFAVIGI NTAFSSKKRR DSVRQTWMPT GEKLKKIEKE KGIVVRFVIG HSATPGGVLD 190 200 210 220 230 240 KAIDEEDSEH KDFLRLKHIE GYHQLSTKTR LYFSTATAMY DAEFYVKVDD DVHVNLGMLV 250 260 270 280 290 300 TTLARYQSRP RIYIGCMKSG PVLSQKGVKY HEPEFWKFGE EGNKYFRHAT GQIYAISKDL 310 320 330 340 350 360 ATYISTNQGI LHRYANEDVS LGAWMLGLEV EHVDERSMCC GTPPDCQWKA QAGNVCAASF 370 380 390 DWSCSGICKS VDRMARVHRA CAEGDTPLAN FRFFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC051630 Genomic DNA. Translation: AAG51207.1. CP002684 Genomic DNA. Translation: AEE31593.1. BX813522 mRNA. No translation available.
IPI	IPI00534656.
PIR	A86458.
RefSeq	NP_174609.1. NM_103068.2.
UniGene	At.69763.
3D structure databases
ProteinModelPortal	Q9C809.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C809.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q9C809.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G33430.1; AT1G33430.1; AT1G33430.
GeneID	840236.
GenomeReviews	Gene locus AT1G33430 in contig CT485782_GR.
KEGG	ath:AT1G33430.
NMPDR	fig|3702.1.peg.3697.
Organism-specific databases
TAIR	At1g33430.
Phylogenomic databases
eggNOG	KOG2288.
GeneTree	EPGT00070000028122.
HOGENOM	HBG318611.
InParanoid	Q9C809.
OMA	WSERSAK.
PhylomeDB	Q9C809.
ProtClustDB	CLSN2679270.
Gene expression databases
Genevestigator	Q9C809.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GT8_ARATH

Accession

Primary (citable) accession number: Q9C809

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program