ID   LACS3_ARATH             Reviewed;         665 AA.
AC   Q9C7W4;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=Long chain acyl-CoA synthetase 3;
DE            EC=6.2.1.3;
GN   Name=LACS3; OrderedLocusNames=At1g64400; ORFNames=F15H21.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND ENZYME ACTIVITY.
RX   PubMed=12177484; DOI=10.1104/pp.003269;
RA   Shockey J.M., Fulda M.S., Browse J.A.;
RT   "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that
RT   participate in fatty acid and glycerolipid metabolism.";
RL   Plant Physiol. 129:1710-1722(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   GENE FAMILY ORGANIZATION.
RX   PubMed=12805634; DOI=10.1104/pp.103.020552;
RA   Shockey J.M., Fulda M.S., Browse J.;
RT   "Arabidopsis contains a large superfamily of acyl-activating enzymes.
RT   Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme
RT   a synthetases.";
RL   Plant Physiol. 132:1065-1076(2003).
CC   -!- FUNCTION: Activation of long-chain fatty acids for both synthesis of
CC       cellular lipids, and degradation via beta-oxidation. Preferentially
CC       uses palmitate, palmitoleate, oleate and linoleate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-
CC         CoA + AMP + diphosphate; Xref=Rhea:RHEA:15421, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:83139, ChEBI:CHEBI:456215; EC=6.2.1.3;
CC         Evidence={ECO:0000269|PubMed:12177484};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AF503753; AAM28870.1; -; mRNA.
DR   EMBL; AC066689; AAG51719.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE34237.1; -; Genomic_DNA.
DR   EMBL; AY039935; AAK64039.1; -; mRNA.
DR   EMBL; AY079314; AAL85045.1; -; mRNA.
DR   PIR; B96668; B96668.
DR   RefSeq; NP_176622.1; NM_105115.4.
DR   AlphaFoldDB; Q9C7W4; -.
DR   SMR; Q9C7W4; -.
DR   STRING; 3702.Q9C7W4; -.
DR   iPTMnet; Q9C7W4; -.
DR   PaxDb; 3702-AT1G64400-1; -.
DR   ProteomicsDB; 238653; -.
DR   EnsemblPlants; AT1G64400.1; AT1G64400.1; AT1G64400.
DR   GeneID; 842748; -.
DR   Gramene; AT1G64400.1; AT1G64400.1; AT1G64400.
DR   KEGG; ath:AT1G64400; -.
DR   Araport; AT1G64400; -.
DR   TAIR; AT1G64400; LACS3.
DR   eggNOG; KOG1256; Eukaryota.
DR   HOGENOM; CLU_000022_45_4_1; -.
DR   InParanoid; Q9C7W4; -.
DR   OMA; HGLTIYS; -.
DR   OrthoDB; 22305at2759; -.
DR   PhylomeDB; Q9C7W4; -.
DR   BioCyc; ARA:AT1G64400-MONOMER; -.
DR   BioCyc; MetaCyc:AT1G64400-MONOMER; -.
DR   UniPathway; UPA00199; -.
DR   PRO; PR:Q9C7W4; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C7W4; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004467; F:long-chain fatty acid-CoA ligase activity; IDA:UniProtKB.
DR   GO; GO:0006631; P:fatty acid metabolic process; TAS:UniProtKB.
DR   CDD; cd05927; LC-FACS_euk; 1.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR045311; LC-FACS_euk.
DR   PANTHER; PTHR43272:SF42; LONG CHAIN ACYL-COA SYNTHETASE 3; 1.
DR   PANTHER; PTHR43272; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
DR   Genevisible; Q9C7W4; AT.
PE   2: Evidence at transcript level;
KW   ATP-binding; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..665
FT                   /note="Long chain acyl-CoA synthetase 3"
FT                   /id="PRO_0000401411"
FT   REGION          495..519
FT                   /note="Fatty acid-binding"
FT                   /evidence="ECO:0000255"
FT   BINDING         228..239
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   665 AA;  74751 MW;  B546A845F101DB6C CRC64;
     MATGRYIVEV EKGKQGVDGG SPSVGPVYRS IYAKDGFPEP PDDLVSAWDI FRLSVEKSPN
     NPMLGRREIV DGKAGKYVWQ TYKEVHNVVI KLGNSIRTIG VGKGDKCGIY GANSPEWIIS
     MEACNAHGLY CVPLYDTLGA GAIEFIICHA EVSLAFAEEN KISELLKTAP KSTKYLKYIV
     SFGEVTNNQR VEAERHRLTI YSWDQFLKLG EGKHYELPEK RRSDVCTIMY TSGTTGDPKG
     VLLTNESIIH LLEGVKKLLK TIDEELTSKD VYLSYLPLAH IFDRVIEELC IYEAASIGFW
     RGDVKILIED IAALKPTVFC AVPRVLERIY TGLQQKLSDG GFVKKKLFNF AFKYKHKNME
     KGQPHEQASP IADKIVFKKV KEGLGGNVRL ILSGAAPLAA HIESFLRVVA CAHVLQGYGL
     TESCGGTFVS IPNELSMLGT VGPPVPNVDI RLESVPEMGY DALASNPRGE ICIRGKTLFS
     GYYKREDLTQ EVFIDGWLHT GDVGEWQPDG AMKIIDRKKN IFKLSQGEYV AVENLENIYS
     HVAAIESIWV YGNSYESYLV AVVCPSKIQI EHWAKEHKVS GDFESICRNQ KTKEFVLGEF
     NRVAKDKKLK GFELIKGVHL DTVPFDMERD LITPSYKMKR PQLLKYYQKE IDEMYKKNRE
     VQLRV
//