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Q9C7U5

- E132_ARATH

UniProt

Q9C7U5 - E132_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 2

Gene

At1g66250

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei272 – 2721NucleophileBy similarity
    Active sitei334 – 3341Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT1G66250-MONOMER.

    Protein family/group databases

    CAZyiCBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 2 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 2
    Short name:
    (1->3)-beta-glucanase 2
    Beta-1,3-endoglucanase 2
    Short name:
    Beta-1,3-glucanase 2
    Gene namesi
    Ordered Locus Names:At1g66250
    ORF Names:T6J19.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G66250.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: TAIR
    2. anchored component of plasma membrane Source: TAIR
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 477457Glucan endo-1,3-beta-glucosidase 2PRO_0000252330Add
    BLAST
    Propeptidei478 – 50528Removed in mature formSequence AnalysisPRO_0000252331Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi180 – 1801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi369 ↔ 432By similarity
    Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence Analysis
    Lipidationi477 – 4771GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    Contains two additional disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9C7U5.
    PRIDEiQ9C7U5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9C7U5.
    GenevestigatoriQ9C7U5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C7U5.
    SMRiQ9C7U5. Positions 32-348, 367-452.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG290245.
    HOGENOMiHOG000238220.
    InParanoidiQ9C7U5.
    OMAiSHGTCVF.
    PhylomeDBiQ9C7U5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view]
    SMARTiSM00768. X8. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9C7U5-1 [UniParc]FASTAAdd to Basket

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    MASLLHLLLL SLSLLVLASA SPSPPADEGS YIGVNIGTDL SDMPHPTQVV    50
    ALLKAQEIRH IRLYNADPGL LIALANTGIK VIISIPNDQL LGIGQSNSTA 100
    ANWVKRNVIA HYPATMITAV SVGSEVLTSL SNAAPVLVSA IKNVHAALLS 150
    ANLDKLIKVS TPLSTSLILD PFPPSQAFFN RSLNAVIVPL LSFLQSTNSY 200
    LMVNVYPYID YMQSNGVIPL DYALFKPIPP NKEAVDANTL VRYSNAFDAM 250
    VDATYFAMAF LNFTNIPVLV TESGWPSKGE TNEPDATLDN ANTYNSNLIR 300
    HVLNKTGTPK RPGIAVSTYI YELYNEDTKA GLSEKNWGLF NANGEPVYVL 350
    RLTNSGSVLA NDTTNQTYCT AREGADTKML QAALDWACGP GKIDCSPIKQ 400
    GETCYEPDNV VAHANYAFDT YYHQTGNNPD ACNFNGVASI TTTDPSHGTC 450
    VFAGSRGNGR NGTSVNITAP SANSTTSSGI RSDLYYSRGI WSILTVMILN 500
    VANIL 505
    Length:505
    Mass (Da):54,206
    Last modified:October 3, 2006 - v2
    Checksum:i1A1CC342B307F29F
    GO

    Sequence cautioni

    The sequence AAG51762.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC066691 Genomic DNA. Translation: AAG51762.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34485.1.
    BX814184 mRNA. No translation available.
    PIRiE96687.
    RefSeqiNP_176799.2. NM_105296.2.
    UniGeneiAt.49486.

    Genome annotation databases

    EnsemblPlantsiAT1G66250.1; AT1G66250.1; AT1G66250.
    GeneIDi842942.
    KEGGiath:AT1G66250.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC066691 Genomic DNA. Translation: AAG51762.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34485.1 .
    BX814184 mRNA. No translation available.
    PIRi E96687.
    RefSeqi NP_176799.2. NM_105296.2.
    UniGenei At.49486.

    3D structure databases

    ProteinModelPortali Q9C7U5.
    SMRi Q9C7U5. Positions 32-348, 367-452.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi Q9C7U5.
    PRIDEi Q9C7U5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G66250.1 ; AT1G66250.1 ; AT1G66250 .
    GeneIDi 842942.
    KEGGi ath:AT1G66250.

    Organism-specific databases

    TAIRi AT1G66250.

    Phylogenomic databases

    eggNOGi NOG290245.
    HOGENOMi HOG000238220.
    InParanoidi Q9C7U5.
    OMAi SHGTCVF.
    PhylomeDBi Q9C7U5.

    Enzyme and pathway databases

    BioCyci ARA:AT1G66250-MONOMER.

    Gene expression databases

    ArrayExpressi Q9C7U5.
    Genevestigatori Q9C7U5.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view ]
    SMARTi SM00768. X8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis."
      Borner G.H.H., Lilley K.S., Stevens T.J., Dupree P.
      Plant Physiol. 132:568-577(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
      Tissue: Callus.

    Entry informationi

    Entry nameiE132_ARATH
    AccessioniPrimary (citable) accession number: Q9C7U5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3