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Protein

Glucan endo-1,3-beta-glucosidase 2

Gene

At1g66250

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei272NucleophileBy similarity1
Active sitei334Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processPlant defense

Enzyme and pathway databases

BioCyciARA:AT1G66250-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 2 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 2
Short name:
(1->3)-beta-glucanase 2
Beta-1,3-endoglucanase 2
Short name:
Beta-1,3-glucanase 2
Gene namesi
Ordered Locus Names:At1g66250
ORF Names:T6J19.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G66250.
TAIRilocus:2205298. AT1G66250.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • plasma membrane Source: TAIR

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000025233021 – 477Glucan endo-1,3-beta-glucosidase 2Add BLAST457
PropeptideiPRO_0000252331478 – 505Removed in mature formSequence analysisAdd BLAST28

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi180N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi361N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi365N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi369 ↔ 432By similarity
Glycosylationi461N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi473N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi477GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9C7U5.
PRIDEiQ9C7U5.

Expressioni

Gene expression databases

GenevisibleiQ9C7U5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G66250.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C7U5.
SMRiQ9C7U5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEQ4. Eukaryota.
ENOG410ZF4V. LUCA.
HOGENOMiHOG000238220.
InParanoidiQ9C7U5.
KOiK19891.
OMAiNGTMVNI.
OrthoDBiEOG093607VJ.
PhylomeDBiQ9C7U5.

Family and domain databases

InterProiView protein in InterPro
IPR000490. Glyco_hydro_17.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
PfamiView protein in Pfam
PF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
SMARTiView protein in SMART
SM00768. X8. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00587. GLYCOSYL_HYDROL_F17. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C7U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLLHLLLL SLSLLVLASA SPSPPADEGS YIGVNIGTDL SDMPHPTQVV
60 70 80 90 100
ALLKAQEIRH IRLYNADPGL LIALANTGIK VIISIPNDQL LGIGQSNSTA
110 120 130 140 150
ANWVKRNVIA HYPATMITAV SVGSEVLTSL SNAAPVLVSA IKNVHAALLS
160 170 180 190 200
ANLDKLIKVS TPLSTSLILD PFPPSQAFFN RSLNAVIVPL LSFLQSTNSY
210 220 230 240 250
LMVNVYPYID YMQSNGVIPL DYALFKPIPP NKEAVDANTL VRYSNAFDAM
260 270 280 290 300
VDATYFAMAF LNFTNIPVLV TESGWPSKGE TNEPDATLDN ANTYNSNLIR
310 320 330 340 350
HVLNKTGTPK RPGIAVSTYI YELYNEDTKA GLSEKNWGLF NANGEPVYVL
360 370 380 390 400
RLTNSGSVLA NDTTNQTYCT AREGADTKML QAALDWACGP GKIDCSPIKQ
410 420 430 440 450
GETCYEPDNV VAHANYAFDT YYHQTGNNPD ACNFNGVASI TTTDPSHGTC
460 470 480 490 500
VFAGSRGNGR NGTSVNITAP SANSTTSSGI RSDLYYSRGI WSILTVMILN

VANIL
Length:505
Mass (Da):54,206
Last modified:October 3, 2006 - v2
Checksum:i1A1CC342B307F29F
GO

Sequence cautioni

The sequence AAG51762 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC066691 Genomic DNA. Translation: AAG51762.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34485.1.
BX814184 mRNA. No translation available.
PIRiE96687.
RefSeqiNP_176799.2. NM_105296.3.
UniGeneiAt.49486.

Genome annotation databases

EnsemblPlantsiAT1G66250.1; AT1G66250.1; AT1G66250.
GeneIDi842942.
GrameneiAT1G66250.1; AT1G66250.1; AT1G66250.
KEGGiath:AT1G66250.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiE132_ARATH
AccessioniPrimary (citable) accession number: Q9C7U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: July 5, 2017
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families