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Protein

Protein argonaute 7

Gene

AGO7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for the processing of 21 nucleotide trans-acting siRNAs (ta-siRNAs) derived from TAS3a transcripts. Associates preferentially with the microRNA (miRNA) miR390 which guides the cleavage of TAS3 precursor RNA. Seems to act as miR390 specific slicer. Associates mainly with small RNAs of 21 nucleotide in length and with a 5' terminal adenosine. Acts in the RDR6/SGS3/DCL4/AGO7 trans-acting siRNA pathway involved in leaf developmental timing. Does not seems to act on leaf polarity. Required for the production of the 30-40nt bacterial-induced long siRNAs (lsiRNA). Involved in antiviral RNA silencing by contributing to efficient viral RNAs clearance. Targets less structured viral RNAs than AGO1 which is capable of targeting RNAs with more compact structures.8 Publications

GO - Molecular functioni

GO - Biological processi

  • gene silencing by miRNA Source: TAIR
  • production of lsiRNA involved in RNA interference Source: TAIR
  • production of ta-siRNAs involved in RNA interference Source: TAIR
  • regulation of development, heterochronic Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • vegetative phase change Source: TAIR
  • viral gene silencing in virus induced gene silencing Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

Plant defense, RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-426486. Small interfering RNA (siRNA) biogenesis.
R-ATH-426496. Post-transcriptional silencing by small RNAs.
R-ATH-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute 7
Alternative name(s):
Protein ZIPPY
Gene namesi
Name:AGO7
Synonyms:ZIP
Ordered Locus Names:At1g69440
ORF Names:F10D13.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G69440.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • intracellular ribonucleoprotein complex Source: UniProtKB-KW
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

First leaves are elongated and curl downward. Increased number of hydathodes per leaf. Accelerated appearance of abaxial trichomes. Presence of stigmatic tissue in the middle of the septum at the apical end of the carpels.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 990990Protein argonaute 7PRO_0000404669Add
BLAST

Proteomic databases

PaxDbiQ9C793.
PRIDEiQ9C793.

Expressioni

Tissue specificityi

Expressed in leaves and floral buds, and at low levels in roots.1 Publication

Gene expression databases

GenevisibleiQ9C793. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G69440.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C793.
SMRiQ9C793. Positions 153-949.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini375 – 484110PAZPROSITE-ProRule annotationAdd
BLAST
Domaini649 – 950302PiwiPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi62 – 7110Poly-Tyr

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
HOGENOMiHOG000116043.
InParanoidiQ9C793.
KOiK11593.
OMAiQCCLYSN.
PhylomeDBiQ9C793.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKTHHHHH STNKHIPSSK SRTPLLHKPY HHHVQTNPPP FLLHPSSHQN
60 70 80 90 100
LNLVASNLPS SYYYYYYCYF YSQFHNSLPP PPPPHLLPLS PPLPPLLPLP
110 120 130 140 150
PPHSMTRFHK SLPVSQVVER KQQHQQKKKI QVSNNKVSGS IAIEEAALVV
160 170 180 190 200
AKRPDFGGQD GSVIYLLANH FLVKFDSSQR IYHYNVEISP QPSKEIARMI
210 220 230 240 250
KQKLVETDRN SFSGVVPAFD GRQNIYSPVE FQGDRLEFFV NLPIPSCKAV
260 270 280 290 300
MNYGDLREKQ PQKKIEKLFR VNMKLVSKFD GKEQRKEGED WAPLPPEYIH
310 320 330 340 350
ALDVILRENP MEKCTSIGRS FYSSSMGGSK EIGGGAVGLR GFFQSLRHTQ
360 370 380 390 400
QGLALNMDLS ITAFHESIGV IAYLQKRLEF LTDLPRNKGR ELSLEEKREV
410 420 430 440 450
EKALKNIRVF VCHRETVQRY RVYGLTEEIT ENIWFPDREG KYLRLMSYFK
460 470 480 490 500
DHYGYEIQFK NLPCLQISRA RPCYLPMELC MICEGQKFLG KLSDDQAAKI
510 520 530 540 550
MKMGCQKPNE RKAIIDKVMT GSVGPSSGNQ TREFNLEVSR EMTLLKGRIL
560 570 580 590 600
QPPKLKLDRP RNLKESKVFK GTRIERWALM SIGGSSDQKS TIPKFINELT
610 620 630 640 650
QKCEHLGVFL SKNTLSSTFF EPSHILNNIS LLESKLKEIQ RAASNNLQLI
660 670 680 690 700
ICVMEKKHKG YGDLKRISET RIGVVTQCCL YPNITKLSSQ FVSNLALKIN
710 720 730 740 750
AKIGGSMTEL YNSIPSHIPR LLRPDEPVIF MGADVTHPHP FDDCSPSVAA
760 770 780 790 800
VVGSINWPEA NRYVSRMRSQ THRQEIIQDL DLMVKELLDD FYKAVKKLPN
810 820 830 840 850
RIIFFRDGVS ETQFKKVLQE ELQSIKTACS KFQDYNPSIT FAVVQKRHHT
860 870 880 890 900
RLFRCDPDHE NIPPGTVVDT VITHPKEFDF YLCSHLGVKG TSRPTHYHIL
910 920 930 940 950
WDENEFTSDE LQRLVYNLCY TFVRCTKPIS IVPPAYYAHL AAYRGRLYIE
960 970 980 990
RSSESNGGSM NPSSVSRVGP PKTIPLPKLS DNVKNLMFYC
Length:990
Mass (Da):113,397
Last modified:June 1, 2001 - v1
Checksum:i8C3A270FA7E51D9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC073178 Genomic DNA. Translation: AAG60096.1.
CP002684 Genomic DNA. Translation: AEE34925.1.
AY394564 mRNA. Translation: AAQ92355.1.
RefSeqiNP_177103.1. NM_105611.4.
UniGeneiAt.35421.

Genome annotation databases

EnsemblPlantsiAT1G69440.1; AT1G69440.1; AT1G69440.
GeneIDi843276.
GrameneiAT1G69440.1; AT1G69440.1; AT1G69440.
KEGGiath:AT1G69440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC073178 Genomic DNA. Translation: AAG60096.1.
CP002684 Genomic DNA. Translation: AEE34925.1.
AY394564 mRNA. Translation: AAQ92355.1.
RefSeqiNP_177103.1. NM_105611.4.
UniGeneiAt.35421.

3D structure databases

ProteinModelPortaliQ9C793.
SMRiQ9C793. Positions 153-949.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G69440.1.

Proteomic databases

PaxDbiQ9C793.
PRIDEiQ9C793.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G69440.1; AT1G69440.1; AT1G69440.
GeneIDi843276.
GrameneiAT1G69440.1; AT1G69440.1; AT1G69440.
KEGGiath:AT1G69440.

Organism-specific databases

TAIRiAT1G69440.

Phylogenomic databases

eggNOGiKOG1041. Eukaryota.
ENOG410XP07. LUCA.
HOGENOMiHOG000116043.
InParanoidiQ9C793.
KOiK11593.
OMAiQCCLYSN.
PhylomeDBiQ9C793.

Enzyme and pathway databases

ReactomeiR-ATH-426486. Small interfering RNA (siRNA) biogenesis.
R-ATH-426496. Post-transcriptional silencing by small RNAs.
R-ATH-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

PROiQ9C793.

Gene expression databases

GenevisibleiQ9C793. AT.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis heterochronic gene ZIPPY is an ARGONAUTE family member."
    Hunter C., Sun H., Poethig R.S.
    Curr. Biol. 13:1734-1739(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7."
    Adenot X., Elmayan T., Lauressergues D., Boutet S., Bouche N., Gasciolli V., Vaucheret H.
    Curr. Biol. 16:927-932(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway."
    Garcia D., Collier S.A., Byrne M.E., Martienssen R.A.
    Curr. Biol. 16:933-938(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Trans-acting siRNA-mediated repression of ETTIN and ARF4 regulates heteroblasty in Arabidopsis."
    Hunter C., Willmann M.R., Wu G., Yoshikawa M., de la Luz Gutierrez-Nava M., Poethig S.R.
    Development 133:2973-2981(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis."
    Xu L., Yang L., Pi L., Liu Q., Ling Q., Wang H., Poethig R.S., Huang H.
    Plant Cell Physiol. 47:853-863(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A novel class of bacteria-induced small RNAs in Arabidopsis."
    Katiyar-Agarwal S., Gao S., Vivian-Smith A., Jin H.
    Genes Dev. 21:3123-3134(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation."
    Montgomery T.A., Howell M.D., Cuperus J.T., Li D., Hansen J.E., Alexander A.L., Chapman E.J., Fahlgren N., Allen E., Carrington J.C.
    Cell 133:128-141(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  10. "Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1."
    Qu F., Ye X., Morris T.J.
    Proc. Natl. Acad. Sci. U.S.A. 105:14732-14737(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAGO7_ARATH
AccessioniPrimary (citable) accession number: Q9C793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.