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Q9C774 (PSD7B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
26S proteasome non-ATPase regulatory subunit 7 homolog B
Alternative name(s):
26S proteasome regulatory subunit RPN8b
Short name=AtRPN8b
Protein MATERNAL EFFECT EMBRYO ARREST 34
Gene names
Name:RPN8B
Synonyms:MEE34
Ordered Locus Names:At3g11270
ORF Names:F11B9.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins By similarity.

Subunit structure

Component of the 19S regulatory particule (RP/PA700) lid subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Ref.1 Ref.5

Sequence similarities

Belongs to the peptidase M67A family.

Contains 1 MPN (JAB/Mov34) domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9C774-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 31031026S proteasome non-ATPase regulatory subunit 7 homolog B
PRO_0000423178

Regions

Domain14 – 123110MPN

Amino acid modifications

Modified residue11N-acetylmethionine Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 122D45B6A9E2A7ED

FASTA31035,085
        10         20         30         40         50         60 
MDVIKTQQIS ARTIEKVIVH PLVLLSIVDH YNRVAKDTSK RVVGVLLGSS SRGTVDVTNS 

        70         80         90        100        110        120 
YAVPFEEDDK DTSIWFLDHN YHESMFHMFK RINAKEHIVG WYSTGPKLRE NDLDVHALFN 

       130        140        150        160        170        180 
GYVPNPVLVI IDVQPKELGI PTKAYYAVEE VKENATQKSQ QVFVHVPTEI AAHEVEEIGV 

       190        200        210        220        230        240 
EHLLRDVKDT TISTLATEVT AKLTALKGLD ARLREIRTYL DLVIEGKLPL NHEILYHLQD 

       250        260        270        280        290        300 
VFNLLPNLNV NELVKAFAVK TNDMMLVIYL SSLIRSVIAL HSLINNKLLN KEHEKAEDSK 

       310 
PVDIPLITES 

« Hide

References

« Hide 'large scale' references
[1]"Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT MET-1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY258412 mRNA. Translation: AAP83300.1.
AC073395 Genomic DNA. Translation: AAG50979.1.
CP002686 Genomic DNA. Translation: AEE75022.1.
AK228032 mRNA. Translation: BAE99993.1.
RefSeqNP_187736.1. NM_111962.4. [Q9C774-1]
UniGeneAt.39785.

3D structure databases

ProteinModelPortalQ9C774.
SMRQ9C774. Positions 14-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9C774. 15 interactions.
STRING3702.AT3G11270.1-P.

Protein family/group databases

MEROPSM67.973.

Proteomic databases

PRIDEQ9C774.

Protocols and materials databases

DNASU820298.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G11270.1; AT3G11270.1; AT3G11270. [Q9C774-1]
GeneID820298.
KEGGath:AT3G11270.

Organism-specific databases

TAIRAT3G11270.

Phylogenomic databases

HOGENOMHOG000209236.
InParanoidQ9C774.
KOK03038.
OMAINAKEHI.
PhylomeDBQ9C774.

Gene expression databases

ArrayExpressQ9C774.
GenevestigatorQ9C774.

Family and domain databases

InterProIPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSD7B_ARATH
AccessionPrimary (citable) accession number: Q9C774
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names