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Q9C774

- PSD7B_ARATH

UniProt

Q9C774 - PSD7B_ARATH

Protein

26S proteasome non-ATPase regulatory subunit 7 homolog B

Gene

RPN8B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.By similarity

    GO - Biological processi

    1. embryo development ending in seed dormancy Source: TAIR
    2. protein catabolic process Source: TAIR

    Enzyme and pathway databases

    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Protein family/group databases

    MEROPSiM67.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    26S proteasome non-ATPase regulatory subunit 7 homolog B
    Alternative name(s):
    26S proteasome regulatory subunit RPN8b
    Short name:
    AtRPN8b
    Protein MATERNAL EFFECT EMBRYO ARREST 34
    Gene namesi
    Name:RPN8B
    Synonyms:MEE34
    Ordered Locus Names:At3g11270
    ORF Names:F11B9.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G11270.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: TAIR
    2. proteasome complex Source: TAIR

    Keywords - Cellular componenti

    Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 31031026S proteasome non-ATPase regulatory subunit 7 homolog BPRO_0000423178Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ9C774.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9C774.
    GenevestigatoriQ9C774.

    Interactioni

    Subunit structurei

    Component of the 19S regulatory particule (RP/PA700) lid subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.2 Publications

    Protein-protein interaction databases

    IntActiQ9C774. 15 interactions.
    STRINGi3702.AT3G11270.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C774.
    SMRiQ9C774. Positions 14-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 123110MPNAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M67A family.Curated
    Contains 1 MPN (JAB/Mov34) domain.Curated

    Phylogenomic databases

    HOGENOMiHOG000209236.
    InParanoidiQ9C774.
    KOiK03038.
    OMAiINAKEHI.
    PhylomeDBiQ9C774.

    Family and domain databases

    InterProiIPR000555. JAMM/MPN+_dom.
    IPR024969. Rpn11/EIF3F_C.
    [Graphical view]
    PfamiPF01398. JAB. 1 hit.
    PF13012. MitMem_reg. 1 hit.
    [Graphical view]
    SMARTiSM00232. JAB_MPN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9C774-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDVIKTQQIS ARTIEKVIVH PLVLLSIVDH YNRVAKDTSK RVVGVLLGSS    50
    SRGTVDVTNS YAVPFEEDDK DTSIWFLDHN YHESMFHMFK RINAKEHIVG 100
    WYSTGPKLRE NDLDVHALFN GYVPNPVLVI IDVQPKELGI PTKAYYAVEE 150
    VKENATQKSQ QVFVHVPTEI AAHEVEEIGV EHLLRDVKDT TISTLATEVT 200
    AKLTALKGLD ARLREIRTYL DLVIEGKLPL NHEILYHLQD VFNLLPNLNV 250
    NELVKAFAVK TNDMMLVIYL SSLIRSVIAL HSLINNKLLN KEHEKAEDSK 300
    PVDIPLITES 310
    Length:310
    Mass (Da):35,085
    Last modified:June 1, 2001 - v1
    Checksum:i122D45B6A9E2A7ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY258412 mRNA. Translation: AAP83300.1.
    AC073395 Genomic DNA. Translation: AAG50979.1.
    CP002686 Genomic DNA. Translation: AEE75022.1.
    AK228032 mRNA. Translation: BAE99993.1.
    RefSeqiNP_187736.1. NM_111962.4. [Q9C774-1]
    UniGeneiAt.39785.

    Genome annotation databases

    EnsemblPlantsiAT3G11270.1; AT3G11270.1; AT3G11270. [Q9C774-1]
    GeneIDi820298.
    KEGGiath:AT3G11270.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY258412 mRNA. Translation: AAP83300.1 .
    AC073395 Genomic DNA. Translation: AAG50979.1 .
    CP002686 Genomic DNA. Translation: AEE75022.1 .
    AK228032 mRNA. Translation: BAE99993.1 .
    RefSeqi NP_187736.1. NM_111962.4. [Q9C774-1 ]
    UniGenei At.39785.

    3D structure databases

    ProteinModelPortali Q9C774.
    SMRi Q9C774. Positions 14-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9C774. 15 interactions.
    STRINGi 3702.AT3G11270.1-P.

    Protein family/group databases

    MEROPSi M67.973.

    Proteomic databases

    PRIDEi Q9C774.

    Protocols and materials databases

    DNASUi 820298.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G11270.1 ; AT3G11270.1 ; AT3G11270 . [Q9C774-1 ]
    GeneIDi 820298.
    KEGGi ath:AT3G11270.

    Organism-specific databases

    TAIRi AT3G11270.

    Phylogenomic databases

    HOGENOMi HOG000209236.
    InParanoidi Q9C774.
    KOi K03038.
    OMAi INAKEHI.
    PhylomeDBi Q9C774.

    Enzyme and pathway databases

    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_107429. Orc1 removal from chromatin.
    REACT_185297. Separation of Sister Chromatids.
    REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_187719. ER-Phagosome pathway.
    REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    ArrayExpressi Q9C774.
    Genevestigatori Q9C774.

    Family and domain databases

    InterProi IPR000555. JAMM/MPN+_dom.
    IPR024969. Rpn11/EIF3F_C.
    [Graphical view ]
    Pfami PF01398. JAB. 1 hit.
    PF13012. MitMem_reg. 1 hit.
    [Graphical view ]
    SMARTi SM00232. JAB_MPN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
      Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
      J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
      Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
      J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT, ACETYLATION AT MET-1.

    Entry informationi

    Entry nameiPSD7B_ARATH
    AccessioniPrimary (citable) accession number: Q9C774
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3