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Protein

Probable inositol transporter 2

Gene

INT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane inositol-proton symporter. Specific for several inositol epimers, such as myoinositol and scylloinositol. D-chiroinositol, mucoinositol, alloinositol and pinitol are also transported with a lower activity. Not active with galactinol and phytate.1 Publication

Enzyme regulationi

Inhibited by nickel and to a lesser extent by cobalt.

Kineticsi

  1. KM=1.16 mM for inositol2 Publications

    pH dependencei

    Optimum pH is 5.5-7.0.2 Publications

    GO - Molecular functioni

    GO - Biological processi

    • glucose import Source: GO_Central
    • glucose transmembrane transport Source: GOC
    • hexose transmembrane transport Source: GO_Central
    • myo-inositol transport Source: TAIR
    • proton transport Source: GOC
    • signaling Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-429593. Inositol transporters.

    Protein family/group databases

    TCDBi2.A.1.1.63. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable inositol transporter 2
    Gene namesi
    Name:INT2
    Ordered Locus Names:At1g30220
    ORF Names:F12P21.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G30220.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621Helical; Name=1Sequence analysisAdd
    BLAST
    Transmembranei71 – 9121Helical; Name=2Sequence analysisAdd
    BLAST
    Transmembranei106 – 12621Helical; Name=3Sequence analysisAdd
    BLAST
    Transmembranei129 – 14921Helical; Name=4Sequence analysisAdd
    BLAST
    Transmembranei156 – 17621Helical; Name=5Sequence analysisAdd
    BLAST
    Transmembranei189 – 20921Helical; Name=6Sequence analysisAdd
    BLAST
    Transmembranei275 – 29521Helical; Name=7Sequence analysisAdd
    BLAST
    Transmembranei315 – 33521Helical; Name=8Sequence analysisAdd
    BLAST
    Transmembranei343 – 36321Helical; Name=9Sequence analysisAdd
    BLAST
    Transmembranei452 – 47221Helical; Name=10Sequence analysisAdd
    BLAST
    Transmembranei490 – 51021Helical; Name=11Sequence analysisAdd
    BLAST
    Transmembranei521 – 54121Helical; Name=12Sequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: TAIR
    • pollen tube Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi399 – 3991C → A: Strongly decreased nickel inhibition; when associated with A-402, A-410 and A-413. 1 Publication
    Mutagenesisi399 – 3991C → S: No effect on inostol transport or nickel inhibition. No effect on inostol transport or nickel inhibition; when associated with S-410. 1 Publication
    Mutagenesisi402 – 4021C → A: Strongly decreased nickel inhibition; when associated with A-399, A-410 and A-413. 1 Publication
    Mutagenesisi410 – 4101C → A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-413. 1 Publication
    Mutagenesisi410 – 4101C → S: No effect on inostol transport or nickel inhibition; when associated with S-399. 1 Publication
    Mutagenesisi413 – 4131C → A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-410. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580Probable inositol transporter 2PRO_0000259876Add
    BLAST

    Proteomic databases

    PaxDbiQ9C757.
    PRIDEiQ9C757.

    Expressioni

    Tissue specificityi

    Expressed in the tapetum, but not in pollen grains. Detected in leaf vascular tissue and in roots.1 Publication

    Gene expression databases

    GenevisibleiQ9C757. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G30220.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C757.
    SMRiQ9C757. Positions 25-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The PSI domain (383-450) is not involved in the plasma membrane targeting and is dispensable for the transport function, but is required for the inhibition by nickel.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0254. Eukaryota.
    ENOG410XNQK. LUCA.
    HOGENOMiHOG000202868.
    InParanoidiQ9C757.
    KOiK08150.
    OMAiCTACLVF.
    PhylomeDBiQ9C757.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 2 hits.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9C757-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEGGIIHGGA DESAFKECFS LTWKNPYVLR LAFSAGIGGL LFGYDTGVIS
    60 70 80 90 100
    GALLYIRDDF KSVDRNTWLQ EMIVSMAVAG AIVGAAIGGW ANDKLGRRSA
    110 120 130 140 150
    ILMADFLFLL GAIIMAAAPN PSLLVVGRVF VGLGVGMASM TAPLYISEAS
    160 170 180 190 200
    PAKIRGALVS TNGFLITGGQ FLSYLINLAF TDVTGTWRWM LGIAGIPALL
    210 220 230 240 250
    QFVLMFTLPE SPRWLYRKGR EEEAKAILRR IYSAEDVEQE IRALKDSVET
    260 270 280 290 300
    EILEEGSSEK INMIKLCKAK TVRRGLIAGV GLQVFQQFVG INTVMYYSPT
    310 320 330 340 350
    IVQLAGFASN RTALLLSLVT AGLNAFGSII SIYFIDRIGR KKLLIISLFG
    360 370 380 390 400
    VIISLGILTG VFYEAATHAP AISSLETQRF NNISCPDYKS AMNTNAWDCM
    410 420 430 440 450
    TCLKASSPSC GYCSSPIGKE HPGACWISDD SVKDLCHNEN RLWYTRGCPS
    460 470 480 490 500
    NFGWFALLGL GLYIIFFSPG MGTVPWIVNS EIYPLRFRGI CGGIAATANW
    510 520 530 540 550
    ISNLIVAQSF LSLTEAIGTS WTFLIFGVIS VIALLFVMVC VPETKGMPME
    560 570 580
    EIEKMLERRS MEFKFWKKKS KLVEKQNQSA
    Length:580
    Mass (Da):63,449
    Last modified:June 1, 2001 - v1
    Checksum:iB8E03518F05EED79
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ973176 mRNA. Translation: CAJ00304.1.
    AC073506 Genomic DNA. Translation: AAG50560.1.
    CP002684 Genomic DNA. Translation: AEE31194.1.
    AY074333 mRNA. Translation: AAL67029.1.
    AY123031 mRNA. Translation: AAM67564.1.
    PIRiD86426.
    RefSeqiNP_174313.1. NM_102761.3.
    UniGeneiAt.40594.

    Genome annotation databases

    EnsemblPlantsiAT1G30220.1; AT1G30220.1; AT1G30220.
    GeneIDi839902.
    GrameneiAT1G30220.1; AT1G30220.1; AT1G30220.
    KEGGiath:AT1G30220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ973176 mRNA. Translation: CAJ00304.1.
    AC073506 Genomic DNA. Translation: AAG50560.1.
    CP002684 Genomic DNA. Translation: AEE31194.1.
    AY074333 mRNA. Translation: AAL67029.1.
    AY123031 mRNA. Translation: AAM67564.1.
    PIRiD86426.
    RefSeqiNP_174313.1. NM_102761.3.
    UniGeneiAt.40594.

    3D structure databases

    ProteinModelPortaliQ9C757.
    SMRiQ9C757. Positions 25-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT1G30220.1.

    Protein family/group databases

    TCDBi2.A.1.1.63. the major facilitator superfamily (mfs).

    Proteomic databases

    PaxDbiQ9C757.
    PRIDEiQ9C757.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G30220.1; AT1G30220.1; AT1G30220.
    GeneIDi839902.
    GrameneiAT1G30220.1; AT1G30220.1; AT1G30220.
    KEGGiath:AT1G30220.

    Organism-specific databases

    TAIRiAT1G30220.

    Phylogenomic databases

    eggNOGiKOG0254. Eukaryota.
    ENOG410XNQK. LUCA.
    HOGENOMiHOG000202868.
    InParanoidiQ9C757.
    KOiK08150.
    OMAiCTACLVF.
    PhylomeDBiQ9C757.

    Enzyme and pathway databases

    ReactomeiR-ATH-429593. Inositol transporters.

    Miscellaneous databases

    PROiQ9C757.

    Gene expression databases

    GenevisibleiQ9C757. AT.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR005828. MFS_sugar_transport_like.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 2 hits.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Arabidopsis INOSITOL TRANSPORTER4 mediates high-affinity H+ symport of myoinositol across the plasma membrane."
      Schneider S., Schneidereit A., Konrad K.R., Hajirezaei M.-R., Gramann M., Hedrich R., Sauer N.
      Plant Physiol. 141:565-577(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The monosaccharide transporter gene family in land plants is ancient and shows differential subfamily expression and expansion across lineages."
      Johnson D.A., Hill J.P., Thomas M.A.
      BMC Evol. Biol. 6:64-64(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    6. "Arabidopsis INOSITOL TRANSPORTER2 mediates H+ symport of different inositol epimers and derivatives across the plasma membrane."
      Schneider S., Schneidereit A., Udvardi P., Hammes U., Gramann M., Dietrich P., Sauer N.
      Plant Physiol. 145:1395-1407(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    7. "Novel PSI domains in plant and animal H+-inositol symporters."
      Dotzauer D., Wolfenstetter S., Eibert D., Schneider S., Dietrich P., Sauer N.
      Traffic 11:767-781(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN, MUTAGENESIS OF CYS-399; CYS-402; CYS-410 AND CYS-413, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiINT2_ARATH
    AccessioniPrimary (citable) accession number: Q9C757
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: June 1, 2001
    Last modified: February 17, 2016
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.