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Protein

Probable inositol transporter 2

Gene

INT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane inositol-proton symporter. Specific for several inositol epimers, such as myoinositol and scylloinositol. D-chiroinositol, mucoinositol, alloinositol and pinitol are also transported with a lower activity. Not active with galactinol and phytate.1 Publication

Enzyme regulationi

Inhibited by nickel and to a lesser extent by cobalt.

Kineticsi

  1. KM=1.16 mM for inositol2 Publications

    pH dependencei

    Optimum pH is 5.5-7.0.2 Publications

    GO - Molecular functioni

    GO - Biological processi

    • glucose import Source: GO_Central
    • myo-inositol transport Source: TAIR
    • signaling Source: TAIR

    Keywordsi

    Biological processSymport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-429593 Inositol transporters

    Protein family/group databases

    TCDBi2.A.1.1.63 the major facilitator superfamily (mfs)

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable inositol transporter 2
    Gene namesi
    Name:INT2
    Ordered Locus Names:At1g30220
    ORF Names:F12P21.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi

    Organism-specific databases

    AraportiAT1G30220
    TAIRilocus:2009832 AT1G30220

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei36 – 56Helical; Name=1Sequence analysisAdd BLAST21
    Transmembranei71 – 91Helical; Name=2Sequence analysisAdd BLAST21
    Transmembranei106 – 126Helical; Name=3Sequence analysisAdd BLAST21
    Transmembranei129 – 149Helical; Name=4Sequence analysisAdd BLAST21
    Transmembranei156 – 176Helical; Name=5Sequence analysisAdd BLAST21
    Transmembranei189 – 209Helical; Name=6Sequence analysisAdd BLAST21
    Transmembranei275 – 295Helical; Name=7Sequence analysisAdd BLAST21
    Transmembranei315 – 335Helical; Name=8Sequence analysisAdd BLAST21
    Transmembranei343 – 363Helical; Name=9Sequence analysisAdd BLAST21
    Transmembranei452 – 472Helical; Name=10Sequence analysisAdd BLAST21
    Transmembranei490 – 510Helical; Name=11Sequence analysisAdd BLAST21
    Transmembranei521 – 541Helical; Name=12Sequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi399C → A: Strongly decreased nickel inhibition; when associated with A-402, A-410 and A-413. 1 Publication1
    Mutagenesisi399C → S: No effect on inostol transport or nickel inhibition. No effect on inostol transport or nickel inhibition; when associated with S-410. 1 Publication1
    Mutagenesisi402C → A: Strongly decreased nickel inhibition; when associated with A-399, A-410 and A-413. 1 Publication1
    Mutagenesisi410C → A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-413. 1 Publication1
    Mutagenesisi410C → S: No effect on inostol transport or nickel inhibition; when associated with S-399. 1 Publication1
    Mutagenesisi413C → A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-410. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002598761 – 580Probable inositol transporter 2Add BLAST580

    Proteomic databases

    PaxDbiQ9C757
    PRIDEiQ9C757

    Expressioni

    Tissue specificityi

    Expressed in the tapetum, but not in pollen grains. Detected in leaf vascular tissue and in roots.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9C757 baseline and differential
    GenevisibleiQ9C757 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G30220.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C757
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The PSI domain (383-450) is not involved in the plasma membrane targeting and is dispensable for the transport function, but is required for the inhibition by nickel.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0254 Eukaryota
    ENOG410XNQK LUCA
    HOGENOMiHOG000202868
    InParanoidiQ9C757
    KOiK08150
    OMAiTTWFFNF
    OrthoDBiEOG093608AZ
    PhylomeDBiQ9C757

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR005828 MFS_sugar_transport-like
    IPR036259 MFS_trans_sf
    IPR003663 Sugar/inositol_transpt
    IPR005829 Sugar_transporter_CS
    PfamiView protein in Pfam
    PF00083 Sugar_tr, 2 hits
    PRINTSiPR00171 SUGRTRNSPORT
    SUPFAMiSSF103473 SSF103473, 2 hits
    TIGRFAMsiTIGR00879 SP, 1 hit
    PROSITEiView protein in PROSITE
    PS50850 MFS, 1 hit
    PS00216 SUGAR_TRANSPORT_1, 1 hit
    PS00217 SUGAR_TRANSPORT_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9C757-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEGGIIHGGA DESAFKECFS LTWKNPYVLR LAFSAGIGGL LFGYDTGVIS
    60 70 80 90 100
    GALLYIRDDF KSVDRNTWLQ EMIVSMAVAG AIVGAAIGGW ANDKLGRRSA
    110 120 130 140 150
    ILMADFLFLL GAIIMAAAPN PSLLVVGRVF VGLGVGMASM TAPLYISEAS
    160 170 180 190 200
    PAKIRGALVS TNGFLITGGQ FLSYLINLAF TDVTGTWRWM LGIAGIPALL
    210 220 230 240 250
    QFVLMFTLPE SPRWLYRKGR EEEAKAILRR IYSAEDVEQE IRALKDSVET
    260 270 280 290 300
    EILEEGSSEK INMIKLCKAK TVRRGLIAGV GLQVFQQFVG INTVMYYSPT
    310 320 330 340 350
    IVQLAGFASN RTALLLSLVT AGLNAFGSII SIYFIDRIGR KKLLIISLFG
    360 370 380 390 400
    VIISLGILTG VFYEAATHAP AISSLETQRF NNISCPDYKS AMNTNAWDCM
    410 420 430 440 450
    TCLKASSPSC GYCSSPIGKE HPGACWISDD SVKDLCHNEN RLWYTRGCPS
    460 470 480 490 500
    NFGWFALLGL GLYIIFFSPG MGTVPWIVNS EIYPLRFRGI CGGIAATANW
    510 520 530 540 550
    ISNLIVAQSF LSLTEAIGTS WTFLIFGVIS VIALLFVMVC VPETKGMPME
    560 570 580
    EIEKMLERRS MEFKFWKKKS KLVEKQNQSA
    Length:580
    Mass (Da):63,449
    Last modified:June 1, 2001 - v1
    Checksum:iB8E03518F05EED79
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ973176 mRNA Translation: CAJ00304.1
    AC073506 Genomic DNA Translation: AAG50560.1
    CP002684 Genomic DNA Translation: AEE31194.1
    AY074333 mRNA Translation: AAL67029.1
    AY123031 mRNA Translation: AAM67564.1
    PIRiD86426
    RefSeqiNP_174313.1, NM_102761.4
    UniGeneiAt.40594

    Genome annotation databases

    EnsemblPlantsiAT1G30220.1; AT1G30220.1; AT1G30220
    GeneIDi839902
    GrameneiAT1G30220.1; AT1G30220.1; AT1G30220
    KEGGiath:AT1G30220

    Similar proteinsi

    Entry informationi

    Entry nameiINT2_ARATH
    AccessioniPrimary (citable) accession number: Q9C757
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: June 1, 2001
    Last modified: May 23, 2018
    This is version 116 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

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