Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate dehydrogenase E1 component subunit beta-2, chloroplastic

Gene

PDH-E1 BETA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142Thiamine pyrophosphateBy similarity1

GO - Molecular functioni

  • pyruvate dehydrogenase (acetyl-transferring) activity Source: TAIR

GO - Biological processi

  • fatty acid biosynthetic process Source: TAIR
  • glycolytic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciARA:AT1G30120-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta-2, chloroplastic (EC:1.2.4.1)
Gene namesi
Name:PDH-E1 BETA
Synonyms:E1-BETA-1
Ordered Locus Names:At1g30120
ORF Names:T2H7.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G30120.
TAIRilocus:2202476. AT1G30120.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 44ChloroplastSequence analysisAdd BLAST44
ChainiPRO_000042137145 – 406Pyruvate dehydrogenase E1 component subunit beta-2, chloroplasticAdd BLAST362

Proteomic databases

PaxDbiQ9C6Z3.
PRIDEiQ9C6Z3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9C6Z3. baseline and differential.
GenevisibleiQ9C6Z3. AT.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

BioGridi25126. 6 interactors.
IntActiQ9C6Z3. 8 interactors.
STRINGi3702.AT1G30120.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C6Z3.
SMRiQ9C6Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0524. Eukaryota.
COG0022. LUCA.
HOGENOMiHOG000281450.
InParanoidiQ9C6Z3.
KOiK00162.
OMAiAVGMAMY.
OrthoDBiEOG09360DU7.
PhylomeDBiQ9C6Z3.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
InterProiView protein in InterPro
IPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
PfamiView protein in Pfam
PF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
SMARTiView protein in SMART
SM00861. Transket_pyr. 1 hit.
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C6Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIIHGAGA ATTTLSTFNS VDSKKLFVAP SRTNLSVRSQ RYIVAGSDAS
60 70 80 90 100
KKSFGSGLRV RHSQKLIPNA VATKEADTSA STGHELLLFE ALQEGLEEEM
110 120 130 140 150
DRDPHVCVMG EDVGHYGGSY KVTKGLADKF GDLRVLDTPI CENAFTGMGI
160 170 180 190 200
GAAMTGLRPV IEGMNMGFLL LAFNQISNNC GMLHYTSGGQ FTIPVVIRGP
210 220 230 240 250
GGVGRQLGAE HSQRLESYFQ SIPGIQMVAC STPYNAKGLM KAAIRSENPV
260 270 280 290 300
ILFEHVLLYN LKEKIPDEDY VCNLEEAEMV RPGEHITILT YSRMRYHVMQ
310 320 330 340 350
AAKTLVNKGY DPEVIDIRSL KPFDLHTIGN SVKKTHRVLI VEECMRTGGI
360 370 380 390 400
GASLTAAINE NFHDYLDAPV MCLSSQDVPT PYAGTLEEWT VVQPAQIVTA

VEQLCQ
Length:406
Mass (Da):44,245
Last modified:June 1, 2001 - v1
Checksum:i6C9F31F3D4290513
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271V → I in AAM65270 (PubMed:9393637).Curated1
Sequence conflicti271V → I in AAB86804 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80186 mRNA. Translation: AAB86804.1.
AC074176 Genomic DNA. Translation: AAG50862.1.
CP002684 Genomic DNA. Translation: AEE31181.1.
AF361583 mRNA. Translation: AAK32751.1.
AY093988 mRNA. Translation: AAM16249.1.
AK221898 mRNA. Translation: BAD94262.1.
AY087733 mRNA. Translation: AAM65270.1.
PIRiC86425.
RefSeqiNP_174304.1. NM_102751.5.
UniGeneiAt.10483.
At.70613.

Genome annotation databases

EnsemblPlantsiAT1G30120.1; AT1G30120.1; AT1G30120.
GeneIDi839891.
GrameneiAT1G30120.1; AT1G30120.1; AT1G30120.
KEGGiath:AT1G30120.

Similar proteinsi

Entry informationi

Entry nameiODPB2_ARATH
AccessioniPrimary (citable) accession number: Q9C6Z3
Secondary accession number(s): O24458
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: June 1, 2001
Last modified: December 20, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names