Q9C6Z1 (NCED5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic
Short name=AtNCED5
EC=1.13.11.51

Gene names

Name:	NCED5
Ordered Locus Names:	At1g30100
ORF Names:	T2H7.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids By similarity.
Catalytic activity	A 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal. 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al. 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subunit structure	Interacts in vitro with VAR3. Ref.6
Subcellular location	Plastid › chloroplast thylakoid membrane Ref.5 Ref.6.
Tissue specificity	Detected only in seeds. Ref.5 Ref.7
Developmental stage	Expressed at later stages of seed maturation. Ref.7
Induction	Low induction by drought stress. Ref.4 Ref.5
Sequence similarities	Belongs to the carotenoid oxygenase family.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	9-cis-epoxycarotenoid dioxygenase activity Traceable author statement Ref.5. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Chloroplast Potential
	Chain	46 – 589	544	Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic		PRO_0000285994
Sites
	Metal binding	287	1	Iron By similarity
	Metal binding	336	1	Iron By similarity
	Metal binding	401	1	Iron By similarity
	Metal binding	576	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C6Z1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 6A79B2BF7EAA8179	FASTA	589	65,337
	10 20 30 40 50 60 MACSYILTPN PTKLNLSFAP SDLDAPSPSS SVSFTNTKPR RRKLSANSVS DTPNLLNFPN 70 80 90 100 110 120 YPSPNPIIPE KDTSRWNPLQ RAASAALDFA ETALLRRERS KPLPKTVDPR HQISGNYAPV 130 140 150 160 170 180 PEQSVKSSLS VDGKIPDCID GVYLRNGANP LFEPVSGHHL FDGDGMVHAV KITNGDASYS 190 200 210 220 230 240 CRFTETERLV QEKQLGSPIF PKAIGELHGH SGIARLMLFY ARGLFGLLNH KNGTGVANAG 250 260 270 280 290 300 LVYFHDRLLA MSEDDLPYQV RVTDNGDLET IGRFDFDGQL SSAMIAHPKI DPVTKELFAL 310 320 330 340 350 360 SYDVVKKPYL KYFKFSPEGE KSPDVEIPLA SPTMMHDFAI TENFVVIPDQ QVVFKLSDMF 370 380 390 400 410 420 LGKSPVKYDG EKISRFGILP RNAKDASEMV WVESPETFCF HLWNAWESPE TDEVVVIGSC 430 440 450 460 470 480 MTPADSIFNE CDEQLNSVLS EIRLNLKTGK STRRTIIPGS VQMNLEAGMV NRNLLGRKTR 490 500 510 520 530 540 YAYLAIAEPW PKVSGFAKVD LSTGEVKNHF YGGKKYGGEP FFLPRGLESD GEDDGYIMSF 550 560 570 580 VHDEESWESE LHIVNAVTLE LEATVKLPSR VPYGFHGTFV NSADMLNQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis." Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K. Plant J. 27:325-333(2001) [PubMed: 11532178] [Abstract] Cited for: INDUCTION BY DROUGHT STRESS.
[5]	"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family." Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R. Plant J. 35:44-56(2003) [PubMed: 12834401] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY DROUGHT STRESS.
[6]	"Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development." Naested H., Holm A., Jenkins T., Nielsen H.B., Harris C.A., Beale M.H., Andersen M., Mant A., Scheller H., Camara B., Mattsson O., Mundy J. J. Cell Sci. 117:4807-4818(2004) [PubMed: 15340011] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH VAR3.
[7]	"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy." Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A. Plant J. 45:309-319(2006) [PubMed: 16412079] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC074176 Genomic DNA. Translation: AAG50855.1. CP002684 Genomic DNA. Translation: AEE31178.1. AK226774 mRNA. Translation: BAE98872.1.
IPI	IPI00530821.
PIR	A86425.
RefSeq	NP_174302.1. NM_102749.2.
UniGene	At.51834.
3D structure databases
ProteinModelPortal	Q9C6Z1.
SMR	Q9C6Z1. Positions 77-589.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C6Z1. 1 interaction.
STRING	Q9C6Z1.
Proteomic databases
PRIDE	Q9C6Z1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G30100.1; AT1G30100.1; AT1G30100.
GeneID	839889.
GenomeReviews	Gene locus AT1G30100 in contig CT485782_GR.
KEGG	ath:AT1G30100.
NMPDR	fig|3702.1.peg.3346.
Organism-specific databases
TAIR	At1g30100.
Phylogenomic databases
eggNOG	KOG1285.
GeneTree	EPGT00050000007653.
HOGENOM	HBG559594.
InParanoid	Q9C6Z1.
OMA	DASEMVW.
PhylomeDB	Q9C6Z1.
ProtClustDB	PLN02258.
Gene expression databases
Genevestigator	Q9C6Z1.
Family and domain databases
InterPro	IPR004294. Carotenoid_Oase. [Graphical view]
KO	K09840.
PANTHER	PTHR10543. Carotenoid_Oase. 1 hit.
Pfam	PF03055. RPE65. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCED5_ARATH

Accession

Primary (citable) accession number: Q9C6Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program