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Q9C6W4 (BGA15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 15

Short name=Lactase 15
EC=3.2.1.23
Gene names
Name:BGAL15
Ordered Locus Names:At1g31740
ORF Names:F27M3.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Ubiquitous, with higher levels in roots and siliques. Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence AEE31387.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 779760Beta-galactosidase 15
PRO_0000293094

Regions

Domain694 – 77986SUEL-type lectin

Sites

Active site1781Proton donor Potential
Active site2471Nucleophile Potential

Amino acid modifications

Glycosylation1481N-linked (GlcNAc...) Potential
Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential
Glycosylation4891N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9C6W4 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 888813DF318890B0

FASTA77986,342
        10         20         30         40         50         60 
MVSLSFILCC VLVSSCAYAT IVSHDGRAIT IDGHRRVLLS GSIHYPRSTT EMWPDLIKKG 

        70         80         90        100        110        120 
KEGSLDAIET YVFWNAHEPT RRQYDFSGNL DLIRFLKTIQ NEGMYGVLRI GPYVCAEWNY 

       130        140        150        160        170        180 
GGFPVWLHNM PGMEFRTTNT AFMNEMQNFT TMIVEMVKKE KLFASQGGPI ILAQIENEYG 

       190        200        210        220        230        240 
NVIGSYGEAG KAYIQWCANM ANSLDVGVPW IMCQQDDAPQ PMLNTCNGYY CDNFSPNNPN 

       250        260        270        280        290        300 
TPKMWTENWT GWYKNWGGKD PHRTTEDVAF AVARFFQKEG TFQNYYMYHG GTNFDRTAGG 

       310        320        330        340        350        360 
PYITTTYDYD APLDEFGNLN QPKYGHLKQL HDVLHAMEKT LTYGNISTVD FGNLVTATVY 

       370        380        390        400        410        420 
QTEEGSSCFI GNVNETSDAK INFQGTSYDV PAWSVSILPD CKTETYNTAK INTQTSVMVK 

       430        440        450        460        470        480 
KANEAENEPS TLKWSWRPEN IDSVLLKGKG ESTMRQLFDQ KVVSNDESDY LWYMTTVNLK 

       490        500        510        520        530        540 
EQDPVLGKNM SLRINSTAHV LHAFVNGQHI GNYRVENGKF HYVFEQDAKF NPGANVITLL 

       550        560        570        580        590        600 
SITVGLPNYG AFFENFSAGI TGPVFIIGRN GDETIVKDLS THKWSYKTGL SGFENQLFSS 

       610        620        630        640        650        660 
ESPSTWSAPL GSEPVVVDLL GLGKGTAWIN GNNIGRYWPA FLSDIDGCSA EYHVPRSFLN 

       670        680        690        700        710        720 
SEGDNTLVLF EEIGGNPSLV NFQTIGVGSV CANVYEKNVL ELSCNGKPIS AIKFASFGNP 

       730        740        750        760        770 
GGDCGSFEKG TCEASNNAAA ILTQECVGKE KCSIDVSEDK FGAAECGALA KRLAVEAIC 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC074360 Genomic DNA. Translation: AAG60136.1.
CP002684 Genomic DNA. Translation: AEE31387.1. Sequence problems.
RefSeqNP_683341.1. NM_148500.1.
UniGeneAt.51863.

3D structure databases

ProteinModelPortalQ9C6W4.
SMRQ9C6W4. Positions 20-689.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G31740.1-P.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G31740.1; AT1G31740.1; AT1G31740.
GeneID840061.
KEGGath:AT1G31740.

Organism-specific databases

TAIRAT1G31740.

Phylogenomic databases

eggNOGCOG1874.
InParanoidQ9C6W4.
PhylomeDBQ9C6W4.

Enzyme and pathway databases

BioCycARA:AT1G31740-MONOMER.

Gene expression databases

GenevestigatorQ9C6W4.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGA15_ARATH
AccessionPrimary (citable) accession number: Q9C6W4
Secondary accession number(s): F4IAX4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names