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Protein

Beta-galactosidase 15

Gene

BGAL15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei178 – 1781Proton donorSequence Analysis
Active sitei247 – 2471NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G31740-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 15 (EC:3.2.1.23)
Short name:
Lactase 15
Gene namesi
Name:BGAL15
Ordered Locus Names:At1g31740
ORF Names:F27M3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G31740.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 779760Beta-galactosidase 15PRO_0000293094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Ubiquitous, with higher levels in roots and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9C6W4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G31740.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C6W4.
SMRiQ9C6W4. Positions 21-359, 501-671.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini694 – 77986SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
InParanoidiQ9C6W4.
PhylomeDBiQ9C6W4.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C6W4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLSFILCC VLVSSCAYAT IVSHDGRAIT IDGHRRVLLS GSIHYPRSTT
60 70 80 90 100
EMWPDLIKKG KEGSLDAIET YVFWNAHEPT RRQYDFSGNL DLIRFLKTIQ
110 120 130 140 150
NEGMYGVLRI GPYVCAEWNY GGFPVWLHNM PGMEFRTTNT AFMNEMQNFT
160 170 180 190 200
TMIVEMVKKE KLFASQGGPI ILAQIENEYG NVIGSYGEAG KAYIQWCANM
210 220 230 240 250
ANSLDVGVPW IMCQQDDAPQ PMLNTCNGYY CDNFSPNNPN TPKMWTENWT
260 270 280 290 300
GWYKNWGGKD PHRTTEDVAF AVARFFQKEG TFQNYYMYHG GTNFDRTAGG
310 320 330 340 350
PYITTTYDYD APLDEFGNLN QPKYGHLKQL HDVLHAMEKT LTYGNISTVD
360 370 380 390 400
FGNLVTATVY QTEEGSSCFI GNVNETSDAK INFQGTSYDV PAWSVSILPD
410 420 430 440 450
CKTETYNTAK INTQTSVMVK KANEAENEPS TLKWSWRPEN IDSVLLKGKG
460 470 480 490 500
ESTMRQLFDQ KVVSNDESDY LWYMTTVNLK EQDPVLGKNM SLRINSTAHV
510 520 530 540 550
LHAFVNGQHI GNYRVENGKF HYVFEQDAKF NPGANVITLL SITVGLPNYG
560 570 580 590 600
AFFENFSAGI TGPVFIIGRN GDETIVKDLS THKWSYKTGL SGFENQLFSS
610 620 630 640 650
ESPSTWSAPL GSEPVVVDLL GLGKGTAWIN GNNIGRYWPA FLSDIDGCSA
660 670 680 690 700
EYHVPRSFLN SEGDNTLVLF EEIGGNPSLV NFQTIGVGSV CANVYEKNVL
710 720 730 740 750
ELSCNGKPIS AIKFASFGNP GGDCGSFEKG TCEASNNAAA ILTQECVGKE
760 770
KCSIDVSEDK FGAAECGALA KRLAVEAIC
Length:779
Mass (Da):86,342
Last modified:June 1, 2001 - v1
Checksum:i888813DF318890B0
GO

Sequence cautioni

The sequence AEE31387.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074360 Genomic DNA. Translation: AAG60136.1.
CP002684 Genomic DNA. Translation: AEE31387.1. Sequence problems.
RefSeqiNP_683341.1. NM_148500.1.
UniGeneiAt.51863.

Genome annotation databases

EnsemblPlantsiAT1G31740.1; AT1G31740.1; AT1G31740.
GeneIDi840061.
KEGGiath:AT1G31740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074360 Genomic DNA. Translation: AAG60136.1.
CP002684 Genomic DNA. Translation: AEE31387.1. Sequence problems.
RefSeqiNP_683341.1. NM_148500.1.
UniGeneiAt.51863.

3D structure databases

ProteinModelPortaliQ9C6W4.
SMRiQ9C6W4. Positions 21-359, 501-671.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G31740.1-P.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G31740.1; AT1G31740.1; AT1G31740.
GeneIDi840061.
KEGGiath:AT1G31740.

Organism-specific databases

TAIRiAT1G31740.

Phylogenomic databases

eggNOGiCOG1874.
InParanoidiQ9C6W4.
PhylomeDBiQ9C6W4.

Enzyme and pathway databases

BioCyciARA:AT1G31740-MONOMER.

Gene expression databases

GenevestigatoriQ9C6W4.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
    Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
    Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGA15_ARATH
AccessioniPrimary (citable) accession number: Q9C6W4
Secondary accession number(s): F4IAX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.