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Protein

3-ketoacyl-CoA synthase 5

Gene

KCS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates mostly the synthesis of VLCFAs from 26 to 30 carbons in length (e.g. C20:1, C26, C28, C30).1 Publication

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.Curated

Enzyme regulationi

Inhibited by K3 herbicides such as alachlor, allidochlor, anilofos, cafenstrole and flufenacet (PubMed:15277688). Strongly inhibited by metazachlor and mefluidide (PubMed:22284369).2 Publications

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei220By similarity1
Active sitei299By similarity1
Active sitei383By similarity1
Active sitei387By similarity1
Active sitei416By similarity1
Active sitei420By similarity1

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • pollen tube development Source: TAIR
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G25450-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 51 Publication (EC:2.3.1.199Curated)
Short name:
KCS-51 Publication
Alternative name(s):
Eceriferum 601 Publication
Very long-chain fatty acid condensing enzyme 51 Publication
Short name:
VLCFA condensing enzyme 51 Publication
Gene namesi
Name:KCS51 Publication
Synonyms:CER601 Publication
Ordered Locus Names:At1g25450Imported
ORF Names:F2J7.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G25450.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 40HelicalSequence analysisAdd BLAST21
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2242737.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002490971 – 4923-ketoacyl-CoA synthase 5Add BLAST492

Proteomic databases

PaxDbiQ9C6L5.
PRIDEiQ9C6L5.

Expressioni

Tissue specificityi

Expressed in siliques, flowers, leaves and seedlings.1 Publication

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Down-regulated by darkness, low temperature, salt, drought and osmotic stress (PubMed:18465198).2 Publications

Gene expression databases

GenevisibleiQ9C6L5. AT.

Interactioni

Protein-protein interaction databases

BioGridi24368. 1 interactor.
STRINGi3702.AT1G25450.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C6L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 365FAESequence analysisAdd BLAST290

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated
Contains 1 FAE (fatty acid elongase) domain.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9C6L5.
KOiK15397.
OMAiIFTAMED.
OrthoDBiEOG093606UZ.
PhylomeDBiQ9C6L5.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9C6L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDFSSSVKL KYVKLGYQYL INNFLTLLLI PVIATVAIEL LRMGPEEILS
60 70 80 90 100
VLNSLHFELL HILCSSFLII FVSTVYFMSK PRTVYLVDYS CYKPPVTCRV
110 120 130 140 150
PFSSFMEHSR LILKDNPKSV EFQMRILERS GLGEETCLPP AIHYIPPTPT
160 170 180 190 200
MESARNEAQM VIFTAMEDLF KNTGLKPKDI DILIVNCSLF SPTPSLSAMI
210 220 230 240 250
INKYKLRSNI KSYNLSGMGC SASLISVDVA RDLLQVHPNS NAIIISTEII
260 270 280 290 300
TPNYYKGNER AMLLPNCLFR MGGAAILLSN RRSDRWRAKY KLCHLVRTHR
310 320 330 340 350
GADDKSYNCV MEQEDKNGNV GINLSKDLMT IAGEALKANI TTIGPLVLPA
360 370 380 390 400
SEQLLFLSSL IGRKIFNPKW KPYIPDFKQA FEHFCIHAGG RAVIDELQKN
410 420 430 440 450
LQLSGEHVEA SRMTLHRFGN TSSSSLWYEL SYIEAQGRMK RNDRVWQIAF
460 470 480 490
GSGFKCNSAV WKCNRTIKTP TDGAWSDCIE RYPVFIPEVV KL
Length:492
Mass (Da):55,653
Last modified:June 1, 2001 - v1
Checksum:i42D21D34E4A0DDEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232D → N in AAM67234 (Ref. 5) Curated1
Sequence conflicti356F → S in AAO42223 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079281 Genomic DNA. Translation: AAG50800.1.
CP002684 Genomic DNA. Translation: AEE30626.1.
BT004205 mRNA. Translation: AAO42223.1.
AY088928 mRNA. Translation: AAM67234.1.
PIRiF86384.
RefSeqiNP_173916.1. NM_102356.4.
UniGeneiAt.48237.

Genome annotation databases

EnsemblPlantsiAT1G25450.1; AT1G25450.1; AT1G25450.
GeneIDi839131.
GrameneiAT1G25450.1; AT1G25450.1; AT1G25450.
KEGGiath:AT1G25450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079281 Genomic DNA. Translation: AAG50800.1.
CP002684 Genomic DNA. Translation: AEE30626.1.
BT004205 mRNA. Translation: AAO42223.1.
AY088928 mRNA. Translation: AAM67234.1.
PIRiF86384.
RefSeqiNP_173916.1. NM_102356.4.
UniGeneiAt.48237.

3D structure databases

ProteinModelPortaliQ9C6L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24368. 1 interactor.
STRINGi3702.AT1G25450.1.

Chemistry databases

ChEMBLiCHEMBL2242737.

Proteomic databases

PaxDbiQ9C6L5.
PRIDEiQ9C6L5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G25450.1; AT1G25450.1; AT1G25450.
GeneIDi839131.
GrameneiAT1G25450.1; AT1G25450.1; AT1G25450.
KEGGiath:AT1G25450.

Organism-specific databases

TAIRiAT1G25450.

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9C6L5.
KOiK15397.
OMAiIFTAMED.
OrthoDBiEOG093606UZ.
PhylomeDBiQ9C6L5.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT1G25450-MONOMER.

Miscellaneous databases

PROiQ9C6L5.

Gene expression databases

GenevisibleiQ9C6L5. AT.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiKCS5_ARATH
AccessioniPrimary (citable) accession number: Q9C6L5
Secondary accession number(s): Q84W57, Q8L8L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.