Q9C6I4 (G2OX7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gibberellin 2-beta-dioxygenase 7
EC=1.14.11.13

Alternative name(s):
GA 2-oxidase 7
Gibberellin 2-beta-hydroxylase 7
Gibberellin 2-oxidase 7

Gene names

Name:	GA2OX7
Ordered Locus Names:	At1g50960
ORF Names:	F8A12.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the 2-beta-hydroxylation of gibberellins (GA) precursors, rendering them unable to be converted to active GAs. Hydroxylates the C20-GA GA12 and GA53, but is not active on C19-GAs, like GA1, GA4, GA9 and GA20. Ref.3
Catalytic activity	Gibberellin 1 + 2-oxoglutarate + O2 = 2-beta-hydroxygibberellin 1 + succinate + CO2.
Cofactor	Iron By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gibberellin metabolic process Inferred from direct assay Ref.3. Source: TAIR response to salt stress Inferred from genetic interaction. Source: TAIR
Molecular function	C-20 gibberellin 2-beta-dioxygenase activity Inferred from direct assay Ref.3. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Gibberellin 2-beta-dioxygenase 7		PRO_0000067308
Regions
	Domain	191 – 291	101	Fe2OG dioxygenase
Sites
	Active site	282	1	Potential
	Metal binding	216	1	Iron By similarity
	Metal binding	218	1	Iron By similarity
	Metal binding	272	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C6I4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BA8A0F6DB4236558	FASTA	336	38,289
	10 20 30 40 50 60 MASQPPFKTN FCSIFGSSFP NSTSESNTNT STIQTSGIKL PVIDLSHLTS GEEVKRKRCV 70 80 90 100 110 120 KQMVAAAKEW GFFQIVNHGI PKDVFEMMLL EEKKLFDQPF SVKVRERFSD LSKNSYRWGN 130 140 150 160 170 180 PSATSPAQYS VSEAFHIILS EVSRISDDRN NLRTIVETYV QEIARVAQMI CEILGKQVNV 190 200 210 220 230 240 SSEYFENIFE LENSFLRLNK YHPSVFGSEV FGLVPHTDTS FLTILSQDQI GGLELENNGQ 250 260 270 280 290 300 WISVKPCLEA LTVNIGDMFQ ALSNGVYQSV RHRVISPANI ERMSIAFFVC PYLETEIDCF 310 320 330 GYPKKYRRFS FREYKEQSEH DVKETGDKVG LSRFLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants." Schomburg F.M., Bizzell C.M., Lee D.J., Zeevaart J.A.D., Amasino R.M. Plant Cell 15:151-163(2003) [PubMed: 12509528] [Abstract] Cited for: FUNCTION, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC079284 Genomic DNA. Translation: AAG50945.1. CP002684 Genomic DNA. Translation: AEE32606.1.
IPI	IPI00519781.
PIR	G96546.
RefSeq	NP_175509.1. NM_103976.1.
UniGene	At.52121.
3D structure databases
ProteinModelPortal	Q9C6I4.
SMR	Q9C6I4. Positions 9-321.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C6I4.
Proteomic databases
PRIDE	Q9C6I4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G50960.1; AT1G50960.1; AT1G50960.
GeneID	841518.
GenomeReviews	Gene locus AT1G50960 in contig CT485782_GR.
KEGG	ath:AT1G50960.
NMPDR	fig|3702.1.peg.4566.
Organism-specific databases
TAIR	At1g50960.
Phylogenomic databases
eggNOG	KOG0143.
GeneTree	EPGT00050000009705.
HOGENOM	HBG592995.
InParanoid	Q9C6I4.
OMA	RVISPAN.
PhylomeDB	Q9C6I4.
ProtClustDB	CLSN2912822.
Enzyme and pathway databases
BioCyc	ARA:AT1G50960-MONOMER. MetaCyc:AT1G50960-MONOMER.
Gene expression databases
Genevestigator	Q9C6I4.
GermOnline	AT1G50960. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G2OX7_ARATH

Accession

Primary (citable) accession number: Q9C6I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program