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Protein

Methylthioribose kinase

Gene

MTK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of methylthioribose into methylthioribose-1-phosphate in the methionine cycle. Contributes to the maintenance of AdoMet homeostasis and is required to sustain high rates of ethylene synthesis.2 Publications

Catalytic activityi

ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate.

pH dependencei

Optimum pH is 8.5-9.0.1 Publication

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 2 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1 (MTN1), 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2 (MTN2)
  2. Methylthioribose kinase (MTK)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46Substrate1 Publication1
Binding sitei63ATP1 Publication1
Binding sitei238Substrate1 Publication1
Binding sitei365Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 48ATP1 Publication5
Nucleotide bindingi117 – 119ATP1 Publication3
Nucleotide bindingi255 – 257ATP1 Publication3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: UniProtKB
  • S-methyl-5-thioribose kinase activity Source: UniProtKB

GO - Biological processi

  • cellular response to ethylene stimulus Source: TAIR
  • cellular response to iron ion Source: TAIR
  • cellular response to nitric oxide Source: TAIR
  • L-methionine biosynthetic process from methylthioadenosine Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G49820-MONOMER.
BRENDAi2.7.1.100. 399.
UniPathwayiUPA00904; UER00872.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribose kinase (EC:2.7.1.100)
Short name:
AtMTK
Short name:
MTR kinase
Gene namesi
Name:MTK
Ordered Locus Names:At1g49820
ORF Names:F10F5.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G49820.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition. Not able to grow with methylthioadenosine (MTA) as unique source of sulfur.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004013632 – 420Methylthioribose kinaseAdd BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9C6D2.
PRIDEiQ9C6D2.

PTM databases

iPTMnetiQ9C6D2.

Expressioni

Gene expression databases

GenevisibleiQ9C6D2. AT.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi26630. 1 interactor.
STRINGi3702.AT1G49820.1.

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 19Combined sources7
Helixi22 – 28Combined sources7
Turni29 – 32Combined sources4
Beta strandi38 – 41Combined sources4
Beta strandi45 – 47Combined sources3
Beta strandi49 – 54Combined sources6
Beta strandi59 – 64Combined sources6
Turni70 – 72Combined sources3
Helixi82 – 96Combined sources15
Helixi98 – 100Combined sources3
Beta strandi104 – 108Combined sources5
Turni109 – 112Combined sources4
Beta strandi113 – 116Combined sources4
Helixi126 – 132Combined sources7
Helixi139 – 152Combined sources14
Turni156 – 158Combined sources3
Helixi161 – 171Combined sources11
Helixi172 – 174Combined sources3
Helixi175 – 184Combined sources10
Turni185 – 187Combined sources3
Helixi188 – 190Combined sources3
Turni200 – 203Combined sources4
Helixi204 – 210Combined sources7
Helixi214 – 229Combined sources16
Beta strandi233 – 235Combined sources3
Helixi241 – 243Combined sources3
Beta strandi244 – 246Combined sources3
Beta strandi251 – 253Combined sources3
Beta strandi260 – 262Combined sources3
Helixi264 – 281Combined sources18
Helixi282 – 285Combined sources4
Helixi293 – 320Combined sources28
Turni321 – 323Combined sources3
Turni331 – 333Combined sources3
Helixi337 – 368Combined sources32
Beta strandi369 – 371Combined sources3
Helixi374 – 377Combined sources4
Helixi382 – 402Combined sources21
Helixi403 – 405Combined sources3
Helixi409 – 418Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PYWX-ray1.90A/B1-420[»]
ProteinModelPortaliQ9C6D2.
SMRiQ9C6D2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9C6D2.

Family & Domainsi

Sequence similaritiesi

Belongs to the methylthioribose kinase family.Curated

Phylogenomic databases

eggNOGiENOG410II9Y. Eukaryota.
COG4857. LUCA.
HOGENOMiHOG000082744.
InParanoidiQ9C6D2.
KOiK00899.
OMAiAHNADFE.
OrthoDBiEOG09360AM5.
PhylomeDBiQ9C6D2.

Family and domain databases

HAMAPiMF_01683. Salvage_MtnK. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR009212. Methylthioribose_kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF031134. MTRK. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR01767. MTRK. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C6D2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEEFTPLN EKSLVDYIKS TPALSSKIGA DKSDDDLVIK EVGDGNLNFV
60 70 80 90 100
FIVVGSSGSL VIKQALPYIR CIGESWPMTK ERAYFEATTL RKHGNLSPDH
110 120 130 140 150
VPEVYHFDRT MALIGMRYLE PPHIILRKGL IAGIEYPFLA DHMSDYMAKT
160 170 180 190 200
LFFTSLLYHD TTEHRRAVTE FCGNVELCRL TEQVVFSDPY RVSTFNRWTS
210 220 230 240 250
PYLDDDAKAV REDSALKLEI AELKSMFCER AQALIHGDLH TGSVMVTQDS
260 270 280 290 300
TQVIDPEFSF YGPMGFDIGA YLGNLILAFF AQDGHATQEN DRKEYKQWIL
310 320 330 340 350
RTIEQTWNLF NKRFIALWDQ NKDGPGEAYL ADIYNNTEVL KFVQENYMRN
360 370 380 390 400
LLHDSLGFGA AKMIRRIVGV AHVEDFESIE EDKRRAICER SALEFAKMLL
410 420
KERRKFKSIG EVVSAIQQQS
Length:420
Mass (Da):48,071
Last modified:June 1, 2001 - v1
Checksum:i92809E787ED28B69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079674 Genomic DNA. Translation: AAG51775.1.
CP002684 Genomic DNA. Translation: AEE32480.1.
AY057512 mRNA. Translation: AAL09753.1.
AY090315 mRNA. Translation: AAL90976.1.
PIRiA96535.
RefSeqiNP_564555.1. NM_103869.4.
UniGeneiAt.20360.

Genome annotation databases

EnsemblPlantsiAT1G49820.1; AT1G49820.1; AT1G49820.
GeneIDi841405.
GrameneiAT1G49820.1; AT1G49820.1; AT1G49820.
KEGGiath:AT1G49820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079674 Genomic DNA. Translation: AAG51775.1.
CP002684 Genomic DNA. Translation: AEE32480.1.
AY057512 mRNA. Translation: AAL09753.1.
AY090315 mRNA. Translation: AAL90976.1.
PIRiA96535.
RefSeqiNP_564555.1. NM_103869.4.
UniGeneiAt.20360.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PYWX-ray1.90A/B1-420[»]
ProteinModelPortaliQ9C6D2.
SMRiQ9C6D2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26630. 1 interactor.
STRINGi3702.AT1G49820.1.

PTM databases

iPTMnetiQ9C6D2.

Proteomic databases

PaxDbiQ9C6D2.
PRIDEiQ9C6D2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G49820.1; AT1G49820.1; AT1G49820.
GeneIDi841405.
GrameneiAT1G49820.1; AT1G49820.1; AT1G49820.
KEGGiath:AT1G49820.

Organism-specific databases

TAIRiAT1G49820.

Phylogenomic databases

eggNOGiENOG410II9Y. Eukaryota.
COG4857. LUCA.
HOGENOMiHOG000082744.
InParanoidiQ9C6D2.
KOiK00899.
OMAiAHNADFE.
OrthoDBiEOG09360AM5.
PhylomeDBiQ9C6D2.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00872.
BioCyciARA:AT1G49820-MONOMER.
BRENDAi2.7.1.100. 399.

Miscellaneous databases

EvolutionaryTraceiQ9C6D2.
PROiQ9C6D2.

Gene expression databases

GenevisibleiQ9C6D2. AT.

Family and domain databases

HAMAPiMF_01683. Salvage_MtnK. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR009212. Methylthioribose_kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF031134. MTRK. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR01767. MTRK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTK_ARATH
AccessioniPrimary (citable) accession number: Q9C6D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.