Gamma carbonic anhydrase 2, mitochondrial precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q9C6B3 (GCA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Gamma carbonic anhydrase 2, mitochondrial
Short name=AtCA2
Short name=GAMMA CA2
EC=4.2.1.-

Alternative name(s):
Transcription factor APFI

Gene names

Name:	GAMMACA2
Synonyms:	APFI
Ordered Locus Names:	At1g47260
ORF Names:	F8G22.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enzyme involved in the catabolism of H₂CO₃ but that does not mediates the reversible hydration of carbon dioxide (Ref.15). Mediates complex I assembly in mitochondria and respiration. Binds HCO(3)-. Required for male fertility during anther development and dehiscence to regulate the secondary thickenings of the endothecial cell wall, probably by modulating H₂O(2)-dependent lignin polymerization. Ref.12 Ref.15 Ref.16
Cofactor	Zinc By similarity.
Subunit structure	Homotrimer. Component of the mitochondrial oxidoreductase respiratory chain complex I; element of the extra matrix-exposed domain, which is attached to the membrane arm of this complex. Interacts with GAMMACAL1 and GAMMACAL2. Ref.5 Ref.10 Ref.12 Ref.13 Ref.15
Subcellular location	Mitochondrion membrane; Peripheral membrane protein; Matrix side. Note: Probably integral to the membrane (Ref.13). Ref.6 Ref.7 Ref.9 Ref.13
Tissue specificity	Pollen (at protein level). Constitutively expressed in roots and leaves, with higher levels in flowers, particularly in tapetal tissue of anthers, inflorescence (IM) and floral meristems (FM). Ref.16
Developmental stage	During flower development, first expressed in anthers and later in seeds. Ref.16
Disruption phenotype	Reduced levels of complex I and supercomplex I + III₂ in mitochondrion. Reduced growth rates and respiration of suspension cell culture. Ref.12
Sequence similarities	Belongs to the gamma-class carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anther dehiscence Inferred from mutant phenotype Ref.16. Source: TAIR photorespiration Traceable author statement Ref.13. Source: TAIR protein homotrimerization Inferred from direct assay Ref.15. Source: UniProtKB regulation of reactive oxygen species metabolic process Inferred from mutant phenotype Ref.16. Source: TAIR response to salt stress Inferred from expression pattern PubMed 17916636. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay Ref.6. Source: TAIR mitochondrial respiratory chain complex I Inferred from direct assay PubMed 18189341 PubMed 20197505. Source: TAIR
Molecular_function	carbonate dehydratase activity Inferred from sequence or structural similarity Ref.13. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GAMMACAL1	Q9FMV1	2	EBI-531995,EBI-532008
GAMMACAL2	Q9SMN1	3	EBI-531995,EBI-532001

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 43

Mitochondrion Potential

Chain

44 – 278

235

Gamma carbonic anhydrase 2, mitochondrial

PRO_0000417611

Regions

Region

86 – 88

Substrate binding By similarity

Region

101 – 102

Substrate binding By similarity

Sites

Metal binding

107

Zinc By similarity

Metal binding

130

Zinc By similarity

Metal binding

135

Zinc By similarity

Binding site

209

Substrate By similarity

Experimental info

Sequence conflict

112

N → T in AAK28403. Ref.1

Sequence conflict

191

K → E in AAK28403. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9C6B3 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 15396A4C3D61F731
Show »

FASTA

278

30,065

        10         20         30         40         50         60 
MGTLGRAIYT VGNWIRGTGQ ALDRVGSLLQ GSHRIEEHLS RHRTLMNVFD KSPLVDKDVF 

        70         80         90        100        110        120 
VAPSASVIGD VQIGKGSSIW YGCVLRGDVN NISVGSGTNI QDNTLVHVAK TNISGKVLPT 

       130        140        150        160        170        180 
LIGDNVTVGH SAVIHGCTVE DDAFVGMGAT LLDGVVVEKH AMVAAGSLVK QNTRIPSGEV 

       190        200        210        220        230        240 
WGGNPAKFMR KLTDEEIVYI SQSAKNYINL AQIHASENSK SFEQIEVERA LRKKYARKDE 

       250        260        270 
DYDSMLGITR ETPPELILPD NVLPGGKPVA KVPSTQYF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"APFI: A novel transcription factor involved in the anther specific expression of nuclear-encoded mitochondrial complex I genes." Zabaleta E., Perales M., Lohrmann J., Walter M., Brennicke A., Harter K., Kudla J. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian and fungal components coupled with plant-specific subunits." Heazlewood J.L., Howell K.A., Millar A.H. Biochim. Biophys. Acta 1604:159-169(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, GENE FAMILY. Strain: cv. Landsberg erecta.
[6]	"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions." Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S. Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions." Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S., Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J., Rolland N. Phytochemistry 65:1693-1707(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
[9]	"Gamma carbonic anhydrases in plant mitochondria." Parisi G., Perales M., Fornasari M.S., Colaneri A., Gonzalez-Schain N., Gomez-Casati D., Zimmermann S., Brennicke A., Araya A., Ferry J.G., Echave J., Zabaleta E. Plant Mol. Biol. 55:193-207(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
[10]	"Gamma carbonic anhydrase like complex interact with plant mitochondrial complex I." Perales M., Parisi G., Fornasari M.S., Colaneri A., Villarreal F., Gonzalez-Schain N., Echave J., Gomez-Casati D., Braun H.-P., Araya A., Zabaleta E. Plant Mol. Biol. 56:947-957(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GAMMACAL1 AND GAMMACAL2.
[11]	"A reference map of the Arabidopsis thaliana mature pollen proteome." Noir S., Braeutigam A., Colby T., Schmidt J., Panstruga R. Biochem. Biophys. Res. Commun. 337:1257-1266(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Disruption of a nuclear gene encoding a mitochondrial gamma carbonic anhydrase reduces complex I and supercomplex I + III2 levels and alters mitochondrial physiology in Arabidopsis." Perales M., Eubel H., Heinemeyer J., Colaneri A., Zabaleta E., Braun H.-P. J. Mol. Biol. 350:263-277(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBUNIT.
[13]	"Carbonic anhydrase subunits form a matrix-exposed domain attached to the membrane arm of mitochondrial complex I in plants." Sunderhaus S., Dudkina N.V., Jaensch L., Klodmann J., Heinemeyer J., Perales M., Zabaleta E., Boekema E.J., Braun H.-P. J. Biol. Chem. 281:6482-6488(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
[14]	"Mapping the Arabidopsis organelle proteome." Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S. Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[15]	"Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon." Martin V., Villarreal F., Miras I., Navaza A., Haouz A., Gonzalez-Lebrero R.M., Kaufman S.B., Zabaleta E. FEBS Lett. 583:3425-3430(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[16]	"Ectopic expression of mitochondrial gamma carbonic anhydrase 2 causes male sterility by anther indehiscence." Villarreal F., Martin V., Colaneri A., Gonzalez-Schain N., Perales M., Martin M., Lombardo C., Braun H.-P., Bartoli C., Zabaleta E. Plant Mol. Biol. 70:471-485(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF249876 mRNA. Translation: AAK28403.1. AC079677 Genomic DNA. Translation: AAG52641.1. CP002684 Genomic DNA. Translation: AEE32144.1. AY045979 mRNA. Translation: AAK76653.1. AY113936 mRNA. Translation: AAM44984.1.
IPI	IPI00544889.
PIR	D96513.
RefSeq	NP_175159.1. NM_103620.3.
UniGene	At.397.
3D structure databases
ProteinModelPortal	Q9C6B3.
SMR	Q9C6B3. Positions 43-219.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C6B3. 64 interactions.
MINT	MINT-7266055.
STRING	3702.AT1G47260.1-P.
Proteomic databases
PRIDE	Q9C6B3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G47260.1; AT1G47260.1; AT1G47260.
GeneID	841129.
KEGG	ath:AT1G47260.
Organism-specific databases
GeneFarm	4351. 438.
TAIR	At1g47260.
Phylogenomic databases
eggNOG	COG0663.
InParanoid	Q9C6B3.
OMA	NSIHIGS.
PhylomeDB	Q9C6B3.
ProtClustDB	PLN02296.
Enzyme and pathway databases
BioCyc	ARA:AT1G47260-MONOMER. MetaCyc:AT1G47260-MONOMER.
Gene expression databases
ArrayExpress	Q9C6B3.
Genevestigator	Q9C6B3.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 3 hits. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCA2_ARATH

Accession

Primary (citable) accession number: Q9C6B3
Secondary accession number(s): Q9C5S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2012
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents