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Protein

Eukaryotic translation initiation factor 3 subunit H

Gene

TIF3H1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential). Regulates translation initiation of specific 5' mRNAs harboring multiple upstream open reading frames (uORFs) in their 5' leader sequence (e.g. BETA-OHASE 2 and LHY) (PubMed:15548739, PubMed:17439654, PubMed:23524850).UniRule annotation3 Publications

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • positive regulation of translational initiation Source: UniProtKB
  • response to abscisic acid Source: UniProtKB
  • response to auxin Source: UniProtKB
  • response to glucose Source: UniProtKB
  • response to maltose Source: UniProtKB
  • response to sucrose Source: UniProtKB

Keywordsi

Molecular functionInitiation factor
Biological processAbscisic acid signaling pathway, Protein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-ATH-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-ATH-72689 Formation of a pool of free 40S subunits
R-ATH-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-ATH-72702 Ribosomal scanning and start codon recognition

Protein family/group databases

MEROPSiM67.971

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit HUniRule annotation
Short name:
eIF3hUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 3UniRule annotation
eIF-3-gamma
eIF3 p38 subunit
Gene namesi
Name:TIF3H1
Ordered Locus Names:At1g10840Imported
ORF Names:T16B5.2Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G10840
TAIRilocus:2196469 AT1G10840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Pleiotropic growth defects throughout development including postembryonic growth retardation, and delayed shoot and root growth, flowering, and senescence. Lethal at 80 percent at the vegetative rosettes stage. Reduced fertility. Compromised translation efficiency of specific 5' mRNA leader sequences. Requires exogenous sugar to transit from seedling to vegetative development, but hypersensitive to elevated levels of exogenous sugars (e.g. sucrose, maltose and glucose). Enhanced sensitivity to abscisic acid (ABA) (PubMed:15548739). Impaired uORF-RNAs polysomal association (PubMed:23524850).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi178S → A: Impaired activation of uORF-mRNA translation. 1 Publication1
Mutagenesisi178S → D: Phosphorylation mimic mutant, up-regulation of uORF-mRNA translation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139631 – 337Eukaryotic translation initiation factor 3 subunit HAdd BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei178Phosphoserine; by ATPK11 Publication1

Post-translational modificationi

In response to auxin (NAA), phosphorylated at Ser-178 by ATPK1 and binds to polysomes via TOR signaling. This phosphorylation is repressed by Torin-1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C5Z2

PTM databases

iPTMnetiQ9C5Z2

Expressioni

Tissue specificityi

Mostly expressed in roots and flowers, and, to a lower extent, in leaves, stems and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9C5Z2 baseline and differential
GenevisibleiQ9C5Z2 AT

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Interacts directly with TIF3A1, TIF3B1, TIF3C1, TIF3E1 and TIF3F1 (PubMed:15548739, PubMed:20444226). Associates with the CSN (COP9 signalosome) complex. Binds to CSN1, CSN7 and CSN8 (PubMed:15548739). Interacts with ATPK1 (PubMed:23524850).UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATPK1P428183EBI-3387106,EBI-8107038

Protein-protein interaction databases

BioGridi22867, 12 interactors
IntActiQ9C5Z2, 4 interactors
MINTiQ9C5Z2
STRINGi3702.AT1G10840.1

Structurei

3D structure databases

ProteinModelPortaliQ9C5Z2
SMRiQ9C5Z2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 158MPNPROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the eIF-3 subunit H family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1560 Eukaryota
ENOG410XSR9 LUCA
HOGENOMiHOG000248787
InParanoidiQ9C5Z2
KOiK03247
OMAiKFNRYQQ
OrthoDBiEOG09360G5C
PhylomeDBiQ9C5Z2

Family and domain databases

CDDicd08065 MPN_eIF3h, 1 hit
HAMAPiMF_03007 eIF3h, 1 hit
InterProiView protein in InterPro
IPR027524 eIF3h
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
PANTHERiPTHR10410:SF3 PTHR10410:SF3, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C5Z2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATMARSFLQ AISKDEAVAP PLRVVQIEGL AVLKIIKHCK EFSPTLVTGQ
60 70 80 90 100
LLGLDVGSVL EVTNCFPFPV RDDDEEIEAD GANYQLEMMR CLREVNVDNN
110 120 130 140 150
TVGWYQSTVL GSYQTVELIE TFMNYQENIK RCVCIIYDPS KADLGVLALK
160 170 180 190 200
ALKLSDSFME LYRGGNFTGE KLREKNFSWM DIFEEIPIKV SNSALVSAFM
210 220 230 240 250
TELETDTPVS QGDYDRLHSS TTPFLENNME FLIKCMDDLS MEQQKFQYYY
260 270 280 290 300
RNLSRQQAQQ QAWLQKRRTE NMARKSAGEE PLPEEDPSNP IFKAIPEPSR
310 320 330
LESFLITNQV SNFCGQINGV AGQNFSRLYL TKALHDN
Length:337
Mass (Da):38,373
Last modified:September 19, 2002 - v2
Checksum:iF9965DA546AC977C
GO
Isoform 2 (identifier: Q9C5Z2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-87: Missing.

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:250
Mass (Da):28,888
Checksum:i6082D3F622A0185E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146V → L in AAG53614 (PubMed:11042177).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0579571 – 87Missing in isoform 2. Add BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285833 mRNA Translation: AAG53614.1
AC007354 Genomic DNA Translation: AAD31329.1
CP002684 Genomic DNA Translation: AEE28653.1
CP002684 Genomic DNA Translation: AEE28654.1
AY054641 mRNA Translation: AAK96832.1
AY081546 mRNA Translation: AAM10108.1
BT000765 mRNA Translation: AAN31904.1
AY087338 mRNA Translation: AAM64888.1
PIRiB86242
RefSeqiNP_563880.1, NM_100960.3 [Q9C5Z2-1]
NP_973808.1, NM_202079.1 [Q9C5Z2-2]
UniGeneiAt.17013

Genome annotation databases

EnsemblPlantsiAT1G10840.1; AT1G10840.1; AT1G10840 [Q9C5Z2-1]
AT1G10840.2; AT1G10840.2; AT1G10840 [Q9C5Z2-2]
GeneIDi837627
GrameneiAT1G10840.1; AT1G10840.1; AT1G10840 [Q9C5Z2-1]
AT1G10840.2; AT1G10840.2; AT1G10840 [Q9C5Z2-2]
KEGGiath:AT1G10840

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEIF3H_ARATH
AccessioniPrimary (citable) accession number: Q9C5Z2
Secondary accession number(s): F4I5Y8, Q9SAB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: April 25, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health