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Protein

Structural maintenance of chromosomes protein 2-1

Gene

SMC2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Also involved in chromosome segregation in meiosis.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 398ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • transporter activity Source: TAIR

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic chromosome condensation Source: InterPro
  • transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2299718. Condensation of Prophase Chromosomes.
R-ATH-2514853. Condensation of Prometaphase Chromosomes.

Names & Taxonomyi

Protein namesi
Recommended name:
Structural maintenance of chromosomes protein 2-1
Short name:
AtSMC2-1
Alternative name(s):
Chromosome-associated protein E-1
Short name:
AtCAP-E1
Protein TITAN 3
Gene namesi
Name:SMC2-1
Synonyms:CAP-E1, TTN3
Ordered Locus Names:At5g62410
ORF Names:MMI9.19, MMI9.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G62410.

Subcellular locationi

  • Nucleus

  • Note: Associates with chromatin.By similarity

GO - Cellular componenti

  • condensin complex Source: TAIR
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11751175Structural maintenance of chromosomes protein 2-1PRO_0000284894Add
BLAST

Proteomic databases

PaxDbiQ9C5Y4.
PRIDEiQ9C5Y4.

PTM databases

iPTMnetiQ9C5Y4.

Expressioni

Tissue specificityi

Highly expressed in roots and young floral buds.1 Publication

Gene expression databases

GenevisibleiQ9C5Y4. AT.

Interactioni

Subunit structurei

Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: CAPH, CAPD2 and CAPG.

Protein-protein interaction databases

STRINGi3702.AT5G62410.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5Y4.
SMRiQ9C5Y4. Positions 2-334, 421-718, 842-1166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 11611160Zinc-hookAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni509 – 672164Flexible hingeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili172 – 508337Sequence analysisAdd
BLAST
Coiled coili673 – 1028356Sequence analysisAdd
BLAST

Domaini

The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.

Sequence similaritiesi

Belongs to the SMC family. SMC2 subfamily.Curated
Contains 1 zinc-hook domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0933. Eukaryota.
COG1196. LUCA.
HOGENOMiHOG000163792.
InParanoidiQ9C5Y4.
KOiK06674.
OMAiCQNGKIP.
PhylomeDBiQ9C5Y4.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR027120. Smc2.
IPR010935. SMC_hinge.
[Graphical view]
PANTHERiPTHR18937:SF9. PTHR18937:SF9. 1 hit.
PfamiPF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005719. SMC. 1 hit.
SMARTiSM00968. SMC_hinge. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C5Y4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHIKEICLEG FKSYATRTVV SGFDPHFNAI TGLNGSGKSN ILDSICFVLG
60 70 80 90 100
ITNLQQVRAA NLQELVYKQG QAGITKATVS VTFDNSERHR SPLGYEEHPE
110 120 130 140 150
ITVTRQIVVG GRNKYLINGK LAQPSQVQNL FHSVQLNVNN PHFLIMQGRI
160 170 180 190 200
TKVLNMKPPE ILSMLEEAAG TRMYENKKEA ALKTLEKKQT KVDEINKLLD
210 220 230 240 250
HEILPALEKL RKEKSQYMQW ANGNAELDRL RRFCIAFEYV QAEKIRDNAV
260 270 280 290 300
LGVGEMKAKL GKIDAETEKT QEEIQEFEKQ IKALTQAKEA SMGGEVKTLS
310 320 330 340 350
EKVDSLAQEM TRESSKLNNK EDTLLGEKEN VEKIVHSIED LKKSVKERAA
360 370 380 390 400
AVKKSEEGAA DLKQRFQELS TTLEECEKEH QGVLAGKSSG DEEKCLEDQL
410 420 430 440 450
RDAKIAVGTA GTELKQLKTK IEHCEKELKE RKSQLMSKLE EAIEVENELG
460 470 480 490 500
ARKNDVEHVK KALESIPYNE GQMEALEKDR GAELEVVQRL EDKVRGLSAQ
510 520 530 540 550
LANFQFTYSD PVRNFDRSKV KGVVAKLIKV KDRSSMTALE VTAGGKLYDV
560 570 580 590 600
VVDSEDTGKQ LLQNGALRRR VTIIPLNKIQ SYVVQPRVQQ ATARLVGKDN
610 620 630 640 650
AELALSLVGY SDELKNAMEY VFGSTFVCKT TDVAKEVAFN RDIRTPSVTL
660 670 680 690 700
EGDIFQPSGL LTGGSRKGGG DRLRKLHDLA EAESELQGHQ KRLADVESQI
710 720 730 740 750
KELQPLQMKF TDVYAQLELK TYDLSLFLKR AEQNEHHKLG EAVKKLEEEL
760 770 780 790 800
EEAKSQIKEK ELAYKNCFDA VSKLENSIKD HDKNREGRLK DLEKNIKTIK
810 820 830 840 850
AQMQAASKDL KSHENEKEKL VMEEEAMKQE QSSLESHLTS LETQISTLTS
860 870 880 890 900
EVDEQRAKVD ALQKIHDESL AELKLIHAKM KECDTQISGF VTDQEKCLQK
910 920 930 940 950
LSDMKLERKK LENEVVRMET DHKDCSVKVD KLVEKHTWIA SEKQLFGKGG
960 970 980 990 1000
TDYDFESCDP YVAREKLEKL QSDQSGLEKR VNKKVMAMFE KAEDEYNALI
1010 1020 1030 1040 1050
SKKNTIENDK SKITKVIEEL DEKKKETLKV TWVKVNQDFG SIFSTLLPGT
1060 1070 1080 1090 1100
MAKLEPPEDG NFLDGLEVRV AFGKVWKQSL SELSGGQRSL LALSLILALL
1110 1120 1130 1140 1150
LFKPAPLYIL DEVDAALDLS HTQNIGRMIR AHFPHSQFIV VSLKEGMFNN
1160 1170
ANVLFRTKFV DGVSTVQRTV TKQTK
Length:1,175
Mass (Da):132,600
Last modified:May 1, 2007 - v2
Checksum:i51EA64A11C3FFF62
GO

Sequence cautioni

The sequence AAG27593.2 differs from that shown. Reason: Frameshift at positions 168 and 174. Curated
The sequence AAK58634.1 differs from that shown. Reason: Frameshift at positions 168 and 174. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071I → V in AAK58634 (PubMed:11846874).Curated
Sequence conflicti107 – 1071I → V in AAG27593 (PubMed:11846874).Curated
Sequence conflicti213 – 2131E → K in AAG53093 (PubMed:12783798).Curated
Sequence conflicti254 – 2541G → GVG in AAK58634 (PubMed:11846874).Curated
Sequence conflicti254 – 2541G → GVG in AAG27593 (PubMed:11846874).Curated
Sequence conflicti439 – 4391L → R in AAK58634 (PubMed:11846874).Curated
Sequence conflicti439 – 4391L → R in AAG27593 (PubMed:11846874).Curated
Sequence conflicti439 – 4391L → R in AAG53093 (PubMed:12783798).Curated
Sequence conflicti602 – 6021E → G in AAG53093 (PubMed:12783798).Curated
Sequence conflicti965 – 9651E → V in AAK58634 (PubMed:11846874).Curated
Sequence conflicti965 – 9651E → V in AAG27593 (PubMed:11846874).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271730 Genomic DNA. Translation: AAG27593.2. Frameshift.
AF271731 mRNA. Translation: AAK58634.1. Frameshift.
AF306547 mRNA. Translation: AAG53093.1.
AB015469, AB019235 Genomic DNA. Translation: BAB11491.1.
CP002688 Genomic DNA. Translation: AED97605.1.
RefSeqiNP_201047.1. NM_125635.2.
UniGeneiAt.19029.

Genome annotation databases

EnsemblPlantsiAT5G62410.1; AT5G62410.1; AT5G62410.
GeneIDi836362.
GrameneiAT5G62410.1; AT5G62410.1; AT5G62410.
KEGGiath:AT5G62410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271730 Genomic DNA. Translation: AAG27593.2. Frameshift.
AF271731 mRNA. Translation: AAK58634.1. Frameshift.
AF306547 mRNA. Translation: AAG53093.1.
AB015469, AB019235 Genomic DNA. Translation: BAB11491.1.
CP002688 Genomic DNA. Translation: AED97605.1.
RefSeqiNP_201047.1. NM_125635.2.
UniGeneiAt.19029.

3D structure databases

ProteinModelPortaliQ9C5Y4.
SMRiQ9C5Y4. Positions 2-334, 421-718, 842-1166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G62410.1.

PTM databases

iPTMnetiQ9C5Y4.

Proteomic databases

PaxDbiQ9C5Y4.
PRIDEiQ9C5Y4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G62410.1; AT5G62410.1; AT5G62410.
GeneIDi836362.
GrameneiAT5G62410.1; AT5G62410.1; AT5G62410.
KEGGiath:AT5G62410.

Organism-specific databases

TAIRiAT5G62410.

Phylogenomic databases

eggNOGiKOG0933. Eukaryota.
COG1196. LUCA.
HOGENOMiHOG000163792.
InParanoidiQ9C5Y4.
KOiK06674.
OMAiCQNGKIP.
PhylomeDBiQ9C5Y4.

Enzyme and pathway databases

ReactomeiR-ATH-2299718. Condensation of Prophase Chromosomes.
R-ATH-2514853. Condensation of Prometaphase Chromosomes.

Miscellaneous databases

PROiQ9C5Y4.

Gene expression databases

GenevisibleiQ9C5Y4. AT.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR024704. SMC.
IPR027120. Smc2.
IPR010935. SMC_hinge.
[Graphical view]
PANTHERiPTHR18937:SF9. PTHR18937:SF9. 1 hit.
PfamiPF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005719. SMC. 1 hit.
SMARTiSM00968. SMC_hinge. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF75553. SSF75553. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Condensin and cohesin knockouts in Arabidopsis exhibit a titan seed phenotype."
    Liu C.-M., McElver J., Tzafrir I., Joosen R., Wittich P., Patton D., Van Lammeren A.A.M., Meinke D.
    Plant J. 29:405-415(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
  2. "Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis."
    Siddiqui N.U., Stronghill P.E., Dengler R.E., Hasenkampf C.A., Riggs C.D.
    Development 130:3283-3295(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiSMC21_ARATH
AccessioniPrimary (citable) accession number: Q9C5Y4
Secondary accession number(s): Q9FJK1, Q9FUY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: February 17, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.