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Protein

Molybdenum cofactor sulfurase

Gene

ABA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Modulates cold stress- and osmotic stress-responsive gene expression by acting as key regulator of abscisic acid (ABA) biosynthesis.UniRule annotation5 Publications

Catalytic activityi

Molybdenum cofactor + L-cysteine + reduced acceptor + 2 H+ = thio-molybdenum cofactor + L-alanine + H2O + oxidized acceptor.UniRule annotation2 Publications

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 Publication

Kineticsi

  1. KM=50 µM for L-cysteine1 Publication
  2. KM=200 µM for L-selenocysteine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei4301

    GO - Molecular functioni

    GO - Biological processi

    • abscisic acid biosynthetic process Source: TAIR
    • auxin-activated signaling pathway Source: TAIR
    • defense response to bacterium Source: TAIR
    • molybdenum incorporation into molybdenum-molybdopterin complex Source: TAIR
    • Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-KW
    • protein import into chloroplast stroma Source: TAIR
    • response to cold Source: TAIR
    • response to heat Source: TAIR
    • response to osmotic stress Source: TAIR
    • response to salt stress Source: TAIR
    • stomatal movement Source: TAIR
    • sugar mediated signaling pathway Source: TAIR

    Keywordsi

    Molecular functionTransferase
    Biological processMolybdenum cofactor biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BRENDAi2.8.1.9 399
    ReactomeiR-ATH-947581 Molybdenum cofactor biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Molybdenum cofactor sulfuraseUniRule annotation (EC:2.8.1.9UniRule annotation2 Publications)
    Short name:
    MCSUniRule annotation
    Short name:
    MOSUniRule annotation
    Short name:
    MoCo sulfuraseUniRule annotation
    Alternative name(s):
    Abscisic acid protein 3
    Low expression of osmotically expressive genes protein 5
    Molybdenum cofactor sulfurtransferaseUniRule annotation
    Gene namesi
    Name:ABA3
    Synonyms:LOS5
    Ordered Locus Names:At1g16540
    ORF Names:F19K19.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G16540
    TAIRilocus:2017943 AT1G16540

    Subcellular locationi

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi106G → E in los5-1; induces a deficience in stress-induced ABA accumulation. 1 Publication1
    Mutagenesisi271K → S: Loss of function. 1 Publication1
    Mutagenesisi430C → A: Induces a strong reduction in enzyme activity. 1 Publication1
    Mutagenesisi469G → E in aba3-1; induces a reduced ABA biosynthesis. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002499581 – 819Molybdenum cofactor sulfuraseAdd BLAST819

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei271N6-(pyridoxal phosphate)lysine1

    Proteomic databases

    PaxDbiQ9C5X8

    PTM databases

    iPTMnetiQ9C5X8

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Inductioni

    Up-regulated in response to drought, salt or ABA treatment.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9C5X8 baseline and differential
    GenevisibleiQ9C5X8 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G16540.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5X8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini650 – 817MOSCUniRule annotationAdd BLAST168

    Sequence similaritiesi

    Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. MOCOS subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiKOG2142 Eukaryota
    COG0520 LUCA
    COG3217 LUCA
    HOGENOMiHOG000029698
    InParanoidiQ9C5X8
    KOiK15631
    OMAiRVNTYDC
    OrthoDBiEOG093603XR
    PhylomeDBiQ9C5X8

    Family and domain databases

    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    HAMAPiMF_03050 MOCOS, 1 hit
    InterProiView protein in InterPro
    IPR000192 Aminotrans_V_dom
    IPR005302 MoCF_Sase_C
    IPR028886 MoCo_sulfurase
    IPR005303 MOSC_N
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    IPR011037 Pyrv_Knase-like_insert_dom_sf
    PfamiView protein in Pfam
    PF00266 Aminotran_5, 2 hits
    PF03473 MOSC, 1 hit
    PF03476 MOSC_N, 1 hit
    SUPFAMiSSF50800 SSF50800, 1 hit
    SSF53383 SSF53383, 2 hits
    PROSITEiView protein in PROSITE
    PS51340 MOSC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9C5X8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEAFLKEFGD YYGYPDGPKN IQEIRDTEFK RLDKGVVYLD HAGSTLYSEL
    60 70 80 90 100
    QMEYIFKDFT SNVFGNPHSQ SDISSATSDL IADARHQVLE YFNASPEDYS
    110 120 130 140 150
    CLFTSGATAA LKLVGETFPW TQDSNFLYTM ENHNSVLGIR EYALAQGASA
    160 170 180 190 200
    CAVDIEEAAN QPGQLTNSGP SIKVKHRAVQ MRNTSKLQKE ESRGNAYNLF
    210 220 230 240 250
    AFPSECNFSG LRFNLDLVKL MKENTETVLQ GSPFSKSKRW MVLIDAAKGC
    260 270 280 290 300
    ATLPPDLSEY PADFVVLSFY KLFGYPTGLG ALLVRNDAAK LLKKTYFSGG
    310 320 330 340 350
    TVAASIADID FVKRRERVEE FFEDGSASFL SIAAIRHGFK LLKSLTPSAI
    360 370 380 390 400
    WMHTTSLSIY VKKKLQALRH GNGAAVCVLY GSENLELSSH KSGPTVTFNL
    410 420 430 440 450
    KRPDGSWFGY LEVEKLASLS GIQLRTGCFC NPGACAKYLE LSHSELRSNV
    460 470 480 490 500
    EAGHICWDDN DVINGKPTGA VRVSFGYMST FEDAKKFIDF IISSFASPPK
    510 520 530 540 550
    KTGNGTVVSG RFPQLPSEDL ESKESFPSHY LKSITVYPIK SCAGFSVIRW
    560 570 580 590 600
    PLCRTGLLHD REWMVQGLTG EILTQKKVPE MSLIKTFIDL EEGLLSVESS
    610 620 630 640 650
    RCEDKLHIRI KSDSYNPRND EFDSHANILE NRNEETRINR WFTNAIGRQC
    660 670 680 690 700
    KLLRYSSSTS KDCLNRNKSP GLCRDLESNI NFANEAQFLL ISEESVADLN
    710 720 730 740 750
    RRLEAKDEDY KRAHEKLNPH RFRPNLVISG GEPYGEDKWK TVKIGDNHFT
    760 770 780 790 800
    SLGGCNRCQM INISNEAGLV KKSNEPLTTL ASYRRVKGKI LFGTLLRYEI
    810
    DEKRQCWIGV GEEVNPDIE
    Length:819
    Mass (Da):91,803
    Last modified:June 1, 2001 - v1
    Checksum:i81FE806B186B4007
    GO

    Sequence cautioni

    The sequence AAG10824 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF325457 mRNA Translation: AAK12939.1
    AY034895 mRNA Translation: AAK58888.1
    AC011808 Genomic DNA Translation: AAG10824.1 Sequence problems.
    CP002684 Genomic DNA Translation: AEE29467.1
    PIRiG86300
    RefSeqiNP_564001.1, NM_101519.3
    UniGeneiAt.18927

    Genome annotation databases

    EnsemblPlantsiAT1G16540.1; AT1G16540.1; AT1G16540
    GeneIDi838224
    GrameneiAT1G16540.1; AT1G16540.1; AT1G16540
    KEGGiath:AT1G16540

    Similar proteinsi

    Entry informationi

    Entry nameiMOCOS_ARATH
    AccessioniPrimary (citable) accession number: Q9C5X8
    Secondary accession number(s): Q9FX72
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: June 1, 2001
    Last modified: May 23, 2018
    This is version 104 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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