Q9C5W9 (ARFR_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auxin response factor 18

Gene names

Name:	ARF18
Ordered Locus Names:	At3g61830
ORF Names:	F15G16.220

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Ref.5
Subunit structure	Homo- and heterodimers By similarity.
Subcellular location	Nucleus.
Domain	Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.
Sequence similarities	Belongs to the ARF family. Contains 1 Aux/IAA-ARF domain. Contains 1 TF-B3 DNA-binding domain.
Sequence caution	The sequence CAB71113.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Auxin response factor 18		PRO_0000111522
Regions
	Domain	490 – 571	82	Aux/IAA-ARF
	DNA binding	128 – 230	103	TF-B3

Sequences

	Sequence		Length	Mass (Da)
	Q9C5W9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EC299D42B1A1C0BD	FASTA	602	67,662
	10 20 30 40 50 60 MASVEGDDDF GSSSSRSYQD QLYTELWKVC AGPLVEVPRA QERVFYFPQG HMEQLVASTN 70 80 90 100 110 120 QGINSEEIPV FDLPPKILCR VLDVTLKAEH ETDEVYAQIT LQPEEDQSEP TSLDPPIVGP 130 140 150 160 170 180 TKQEFHSFVK ILTASDTSTH GGFSVLRKHA TECLPSLDMT QATPTQELVT RDLHGFEWRF 190 200 210 220 230 240 KHIFRGQPRR HLLTTGWSTF VSSKRLVAGD AFVFLRGENG DLRVGVRRLA RHQSTMPTSV 250 260 270 280 290 300 ISSQSMHLGV LATASHAVRT TTIFVVFYKP RISQFIVGVN KYMEAIKHGF SLGTRFRMRF 310 320 330 340 350 360 EGEESPERIF TGTIVGSGDL SSQWPASKWR SLQVQWDEPT TVQRPDKVSP WEIEPFLATS 370 380 390 400 410 420 PISTPAQQPQ SKCKRSRPIE PSVKTPAPPS FLYSLPQSQD SINASLKLFQ DPSLERISGG 430 440 450 460 470 480 YSSNNSFKPE TPPPPTNCSY RLFGFDLTSN SPAPIPQDKQ PMDTCGAAKC QEPITPTSMS 490 500 510 520 530 540 EQKKQQTSRS RTKVQMQGIA VGRAVDLTLL KSYDELIDEL EEMFEIQGQL LARDKWIVVF 550 560 570 580 590 600 TDDEGDMMLA GDDPWNEFCK MAKKIFIYSS DEVKKMTTKL KISSSLENEE YGNESFENRS RG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 409-602. Strain: cv. Columbia.
[5]	"Auxin-responsive gene expression: genes, promoters and regulatory factors." Hagen G., Guilfoyle T.J. Plant Mol. Biol. 49:373-385(2002) [PubMed: 12036261] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[6]	"The plant B3 superfamily." Swaminathan K., Peterson K., Jack T. Trends Plant Sci. 13:647-655(2008) [PubMed: 18986826] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL132959 Genomic DNA. Translation: CAB71113.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE80264.1. AF334717 mRNA. Translation: AAG50095.1. AY059746 mRNA. Translation: AAL24094.1. AY070463 mRNA. Translation: AAL49929.1. AY091392 mRNA. Translation: AAM14331.1. AK229945 mRNA. Translation: BAF01771.1.
IPI	IPI00524053.
PIR	T47975.
RefSeq	NP_567119.1. NM_116048.2.
UniGene	At.17167. At.69165.
3D structure databases
ProteinModelPortal	Q9C5W9.
SMR	Q9C5W9. Positions 123-232.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C5W9. 5 interactions.
Proteomic databases
PRIDE	Q9C5W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G61830.1; AT3G61830.1; AT3G61830.
GeneID	825356.
GenomeReviews	Gene locus AT3G61830 in contig BA000014_GR.
KEGG	ath:AT3G61830.
NMPDR	fig|3702.1.peg.17561.
Organism-specific databases
GeneFarm	3414. 345.
TAIR	At3g61830.
Phylogenomic databases
GeneTree	EPGT00070000028018.
HOGENOM	HBG746399.
InParanoid	Q9C5W9.
OMA	TASHAVR.
PhylomeDB	Q9C5W9.
ProtClustDB	CLSN2917487.
Gene expression databases
ArrayExpress	Q9C5W9.
Genevestigator	Q9C5W9.
GermOnline	AT3G61830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. IPR010525. Auxin_resp. IPR003340. B3_DNA-bd. IPR015300. DNA-bd_pseudobarrel. [Graphical view]
Gene3D	G3DSA:2.40.330.10. G3DSA:2.40.330.10. 1 hit.
Pfam	PF02309. AUX_IAA. 1 hit. PF06507. Auxin_resp. 1 hit. PF02362. B3. 1 hit. [Graphical view]
SMART	SM01019. B3. 1 hit. [Graphical view]
SUPFAM	SSF101936. Restrict_endonuc_II_EcoRII_N. 1 hit.
PROSITE	PS50863. B3. 1 hit. PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARFR_ARATH

Accession

Primary (citable) accession number: Q9C5W9
Secondary accession number(s): Q0WM85, Q9M355

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program