Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9C5W3 (NADK2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase 2, chloroplastic

Short name=AtNADK-2
EC=2.7.1.23
Gene names
Name:NADK2
Ordered Locus Names:At1g21640
ORF Names:F8K7.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length985 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in chlorophyll synthesis and chloroplast protection against oxidative damage. Ref.6

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Subcellular location

Plastidchloroplast Ref.6.

Tissue specificity

Expressed in leaves. Ref.5

Developmental stage

Expressed during development from young seedlings to flowering plants. Ref.4

Sequence similarities

Belongs to the NAD kinase family.

Biophysicochemical properties

Kinetic parameters:

Measured at pH 7.9 and 25 degrees Celsius for all experiments.

KM=430 µM for NAD Ref.4

KM=740 µM for ATP

Vmax=14.3 µmol/h/mg enzyme with ATP as substrate

pH dependence:

Optimum pH is 7.9 at 25 degrees Celsius.

Sequence caution

The sequence AAD41416.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandATP-binding
Calmodulin-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9C5W3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6161Chloroplast Potential
Chain62 – 985924NAD kinase 2, chloroplastic HAMAP-Rule MF_00361
PRO_0000233705

Regions

Region335 – 38046Calmodulin-binding HAMAP-Rule MF_00361

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: CF9BDFD804AF5C0E

FASTA985109,188
        10         20         30         40         50         60 
MFLCFCPCHV PIMSRLSPAT GISSRLRFSI GLSSDGRLIP FGFRFRRNDV PFKRRLRFVI 

        70         80         90        100        110        120 
RAQLSEAFSP DLGLDSQAVK SRDTSNLPWI GPVPGDIAEV EAYCRIFRSA ERLHGALMET 

       130        140        150        160        170        180 
LCNPVTGECR VPYDFSPEEK PLLEDKIVSV LGCILSLLNK GRKEILSGRS SSMNSFNLDD 

       190        200        210        220        230        240 
VGVAEESLPP LAVFRGEMKR CCESLHIALE NYLTPDDERS GIVWRKLQKL KNVCYDAGFP 

       250        260        270        280        290        300 
RSDNYPCQTL FANWDPIYSS NTKEDIDSYE SEIAFWRGGQ VTQEGLKWLI ENGFKTIVDL 

       310        320        330        340        350        360 
RAEIVKDTFY QTALDDAISL GKITVVQIPI DVRMAPKAEQ VELFASIVSD SSKRPIYVHS 

       370        380        390        400        410        420 
KEGVWRTSAM VSRWKQYMTR PITKEIPVSE ESKRREVSET KLGSNAVVSG KGVPDEQTDK 

       430        440        450        460        470        480 
VSEINEVDSR SASSQSKESG RFEGDTSASE FNMVSDPLKS QVPPGNIFSR KEMSKFLKSK 

       490        500        510        520        530        540 
SIAPAGYLTN PSKILGTVPT PQFSYTGVTN GNQIVDKDSI RRLAETGNSN GTLLPTSSQS 

       550        560        570        580        590        600 
LDFGNGKFSN GNVHASDNTN KSISDNRGNG FSAAPIAVPP SDNLSRAVGS HSVRESQTQR 

       610        620        630        640        650        660 
NNSGSSSDSS DDEAGAIEGN MCASATGVVR VQSRKKAEMF LVRTDGVSCT REKVTESSLA 

       670        680        690        700        710        720 
FTHPSTQQQM LLWKTTPKTV LLLKKLGQEL MEEAKEAASF LYHQENMNVL VEPEVHDVFA 

       730        740        750        760        770        780 
RIPGFGFVQT FYIQDTSDLH ERVDFVACLG GDGVILHASN LFKGAVPPVV SFNLGSLGFL 

       790        800        810        820        830        840 
TSHPFEDFRQ DLKRVIHGNN TLDGVYITLR MRLRCEIYRK GKAMPGKVFD VLNEIVVDRG 

       850        860        870        880        890        900 
SNPYLSKIEC YEHDRLITKV QGDGVIVATP TGSTAYSTAA GGSMVHPNVP CMLFTPICPH 

       910        920        930        940        950        960 
SLSFRPVILP DSAKLELKIP DDARSNAWVS FDGKRRQQLS RGDSVRIYMS QHPLPTVNKS 

       970        980 
DQTGDWFRSL IRCLNWNERL DQKAL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform."
Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A.
Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, CALMODULIN-BINDING DOMAIN.
[5]"Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana."
Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M.
Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection."
Chai M.-F., Chen Q.-J., An R., Chen Y.-M., Chen J., Wang X.-C.
Plant Mol. Biol. 59:553-564(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007727 Genomic DNA. Translation: AAD41416.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30130.1.
AF337912 mRNA. Translation: AAG60064.1.
AY099794 mRNA. Translation: AAM20645.1.
PIRD86349.
RefSeqNP_564145.1. NM_102013.4. [Q9C5W3-1]
UniGeneAt.28688.
At.47967.

3D structure databases

ProteinModelPortalQ9C5W3.
SMRQ9C5W3. Positions 664-975.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G21640.1-P.

Proteomic databases

PaxDbQ9C5W3.
PRIDEQ9C5W3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G21640.1; AT1G21640.1; AT1G21640. [Q9C5W3-1]
GeneID838766.
KEGGath:AT1G21640.

Organism-specific databases

TAIRAT1G21640.

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000029602.
InParanoidQ9C5W3.
PhylomeDBQ9C5W3.

Enzyme and pathway databases

BioCycARA:GQT-1835-MONOMER.

Gene expression databases

ArrayExpressQ9C5W3.
GenevestigatorQ9C5W3.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
3.90.190.10. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
IPR029021. Prot-tyrosine_phosphatase-like.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
SSF52799. SSF52799. 1 hit.
ProtoNetSearch...

Other

PROQ9C5W3.

Entry information

Entry nameNADK2_ARATH
AccessionPrimary (citable) accession number: Q9C5W3
Secondary accession number(s): Q9XI15
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names