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Q9C5W3

- NADK2_ARATH

UniProt

Q9C5W3 - NADK2_ARATH

Protein

NAD kinase 2, chloroplastic

Gene

NADK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Involved in chlorophyll synthesis and chloroplast protection against oxidative damage.1 Publication

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.

    Kineticsi

    Measured at pH 7.9 and 25 degrees Celsius for all experiments.

    1. KM=430 µM for NAD1 Publication
    2. KM=740 µM for ATP1 Publication

    Vmax=14.3 µmol/h/mg enzyme with ATP as substrate1 Publication

    pH dependencei

    Optimum pH is 7.9 at 25 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. NAD+ kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1835-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinase 2, chloroplastic (EC:2.7.1.23)
    Short name:
    AtNADK-2
    Gene namesi
    Name:NADK2
    Ordered Locus Names:At1g21640
    ORF Names:F8K7.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G21640.

    Subcellular locationi

    Plastidchloroplast 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6161ChloroplastSequence AnalysisAdd
    BLAST
    Chaini62 – 985924NAD kinase 2, chloroplasticPRO_0000233705Add
    BLAST

    Proteomic databases

    PaxDbiQ9C5W3.
    PRIDEiQ9C5W3.

    Expressioni

    Tissue specificityi

    Expressed in leaves.1 Publication

    Developmental stagei

    Expressed during development from young seedlings to flowering plants.1 Publication

    Gene expression databases

    ArrayExpressiQ9C5W3.
    GenevestigatoriQ9C5W3.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G21640.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5W3.
    SMRiQ9C5W3. Positions 664-975.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni335 – 38046Calmodulin-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000029602.
    InParanoidiQ9C5W3.
    PhylomeDBiQ9C5W3.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    3.90.190.10. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    IPR029021. Prot-tyrosine_phosphatase-like.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    SSF52799. SSF52799. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9C5W3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFLCFCPCHV PIMSRLSPAT GISSRLRFSI GLSSDGRLIP FGFRFRRNDV    50
    PFKRRLRFVI RAQLSEAFSP DLGLDSQAVK SRDTSNLPWI GPVPGDIAEV 100
    EAYCRIFRSA ERLHGALMET LCNPVTGECR VPYDFSPEEK PLLEDKIVSV 150
    LGCILSLLNK GRKEILSGRS SSMNSFNLDD VGVAEESLPP LAVFRGEMKR 200
    CCESLHIALE NYLTPDDERS GIVWRKLQKL KNVCYDAGFP RSDNYPCQTL 250
    FANWDPIYSS NTKEDIDSYE SEIAFWRGGQ VTQEGLKWLI ENGFKTIVDL 300
    RAEIVKDTFY QTALDDAISL GKITVVQIPI DVRMAPKAEQ VELFASIVSD 350
    SSKRPIYVHS KEGVWRTSAM VSRWKQYMTR PITKEIPVSE ESKRREVSET 400
    KLGSNAVVSG KGVPDEQTDK VSEINEVDSR SASSQSKESG RFEGDTSASE 450
    FNMVSDPLKS QVPPGNIFSR KEMSKFLKSK SIAPAGYLTN PSKILGTVPT 500
    PQFSYTGVTN GNQIVDKDSI RRLAETGNSN GTLLPTSSQS LDFGNGKFSN 550
    GNVHASDNTN KSISDNRGNG FSAAPIAVPP SDNLSRAVGS HSVRESQTQR 600
    NNSGSSSDSS DDEAGAIEGN MCASATGVVR VQSRKKAEMF LVRTDGVSCT 650
    REKVTESSLA FTHPSTQQQM LLWKTTPKTV LLLKKLGQEL MEEAKEAASF 700
    LYHQENMNVL VEPEVHDVFA RIPGFGFVQT FYIQDTSDLH ERVDFVACLG 750
    GDGVILHASN LFKGAVPPVV SFNLGSLGFL TSHPFEDFRQ DLKRVIHGNN 800
    TLDGVYITLR MRLRCEIYRK GKAMPGKVFD VLNEIVVDRG SNPYLSKIEC 850
    YEHDRLITKV QGDGVIVATP TGSTAYSTAA GGSMVHPNVP CMLFTPICPH 900
    SLSFRPVILP DSAKLELKIP DDARSNAWVS FDGKRRQQLS RGDSVRIYMS 950
    QHPLPTVNKS DQTGDWFRSL IRCLNWNERL DQKAL 985
    Length:985
    Mass (Da):109,188
    Last modified:June 1, 2001 - v1
    Checksum:iCF9BDFD804AF5C0E
    GO

    Sequence cautioni

    The sequence AAD41416.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007727 Genomic DNA. Translation: AAD41416.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE30130.1.
    AF337912 mRNA. Translation: AAG60064.1.
    AY099794 mRNA. Translation: AAM20645.1.
    PIRiD86349.
    RefSeqiNP_564145.1. NM_102013.4. [Q9C5W3-1]
    UniGeneiAt.28688.
    At.47967.

    Genome annotation databases

    EnsemblPlantsiAT1G21640.1; AT1G21640.1; AT1G21640. [Q9C5W3-1]
    GeneIDi838766.
    KEGGiath:AT1G21640.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007727 Genomic DNA. Translation: AAD41416.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE30130.1 .
    AF337912 mRNA. Translation: AAG60064.1 .
    AY099794 mRNA. Translation: AAM20645.1 .
    PIRi D86349.
    RefSeqi NP_564145.1. NM_102013.4. [Q9C5W3-1 ]
    UniGenei At.28688.
    At.47967.

    3D structure databases

    ProteinModelPortali Q9C5W3.
    SMRi Q9C5W3. Positions 664-975.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G21640.1-P.

    Proteomic databases

    PaxDbi Q9C5W3.
    PRIDEi Q9C5W3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G21640.1 ; AT1G21640.1 ; AT1G21640 . [Q9C5W3-1 ]
    GeneIDi 838766.
    KEGGi ath:AT1G21640.

    Organism-specific databases

    TAIRi AT1G21640.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000029602.
    InParanoidi Q9C5W3.
    PhylomeDBi Q9C5W3.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1835-MONOMER.

    Miscellaneous databases

    PROi Q9C5W3.

    Gene expression databases

    ArrayExpressi Q9C5W3.
    Genevestigatori Q9C5W3.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    3.90.190.10. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    IPR029021. Prot-tyrosine_phosphatase-like.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    SSF52799. SSF52799. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform."
      Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A.
      Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, CALMODULIN-BINDING DOMAIN.
    5. "Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana."
      Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M.
      Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection."
      Chai M.-F., Chen Q.-J., An R., Chen Y.-M., Chen J., Wang X.-C.
      Plant Mol. Biol. 59:553-564(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiNADK2_ARATH
    AccessioniPrimary (citable) accession number: Q9C5W3
    Secondary accession number(s): Q9XI15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3