Q9C5W3 (NADK2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD kinase 2, chloroplastic
Short name=AtNADK-2
EC=2.7.1.23

Gene names

Name:	NADK2
Ordered Locus Names:	At1g21640
ORF Names:	F8K7.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	985 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in chlorophyll synthesis and chloroplast protection against oxidative damage. Ref.6
Catalytic activity	ATP + NAD+ = ADP + NADP+.
Subcellular location	Plastid › chloroplast Ref.6.
Tissue specificity	Expressed in leaves. Ref.5
Developmental stage	Expressed during development from young seedlings to flowering plants. Ref.4
Sequence similarities	Belongs to the NAD kinase family.
Biophysicochemical properties	Kinetic parameters: Measured at pH 7.9 and 25 degrees Celsius for all experiments. KM=430 µM for NAD Ref.4 KM=740 µM for ATP Vmax=14.3 µmol/h/mg enzyme with ATP as substrate pH dependence: Optimum pH is 7.9 at 25 degrees Celsius.
Sequence caution	The sequence AAD41416.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Calmodulin-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9C5W3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 61	61	Chloroplast Potential
	Chain	62 – 985	924	NAD kinase 2, chloroplastic		PRO_0000233705
Regions
	Region	335 – 380	46	Calmodulin-binding

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CF9BDFD804AF5C0E	FASTA	985	109,188
	10 20 30 40 50 60 MFLCFCPCHV PIMSRLSPAT GISSRLRFSI GLSSDGRLIP FGFRFRRNDV PFKRRLRFVI 70 80 90 100 110 120 RAQLSEAFSP DLGLDSQAVK SRDTSNLPWI GPVPGDIAEV EAYCRIFRSA ERLHGALMET 130 140 150 160 170 180 LCNPVTGECR VPYDFSPEEK PLLEDKIVSV LGCILSLLNK GRKEILSGRS SSMNSFNLDD 190 200 210 220 230 240 VGVAEESLPP LAVFRGEMKR CCESLHIALE NYLTPDDERS GIVWRKLQKL KNVCYDAGFP 250 260 270 280 290 300 RSDNYPCQTL FANWDPIYSS NTKEDIDSYE SEIAFWRGGQ VTQEGLKWLI ENGFKTIVDL 310 320 330 340 350 360 RAEIVKDTFY QTALDDAISL GKITVVQIPI DVRMAPKAEQ VELFASIVSD SSKRPIYVHS 370 380 390 400 410 420 KEGVWRTSAM VSRWKQYMTR PITKEIPVSE ESKRREVSET KLGSNAVVSG KGVPDEQTDK 430 440 450 460 470 480 VSEINEVDSR SASSQSKESG RFEGDTSASE FNMVSDPLKS QVPPGNIFSR KEMSKFLKSK 490 500 510 520 530 540 SIAPAGYLTN PSKILGTVPT PQFSYTGVTN GNQIVDKDSI RRLAETGNSN GTLLPTSSQS 550 560 570 580 590 600 LDFGNGKFSN GNVHASDNTN KSISDNRGNG FSAAPIAVPP SDNLSRAVGS HSVRESQTQR 610 620 630 640 650 660 NNSGSSSDSS DDEAGAIEGN MCASATGVVR VQSRKKAEMF LVRTDGVSCT REKVTESSLA 670 680 690 700 710 720 FTHPSTQQQM LLWKTTPKTV LLLKKLGQEL MEEAKEAASF LYHQENMNVL VEPEVHDVFA 730 740 750 760 770 780 RIPGFGFVQT FYIQDTSDLH ERVDFVACLG GDGVILHASN LFKGAVPPVV SFNLGSLGFL 790 800 810 820 830 840 TSHPFEDFRQ DLKRVIHGNN TLDGVYITLR MRLRCEIYRK GKAMPGKVFD VLNEIVVDRG 850 860 870 880 890 900 SNPYLSKIEC YEHDRLITKV QGDGVIVATP TGSTAYSTAA GGSMVHPNVP CMLFTPICPH 910 920 930 940 950 960 SLSFRPVILP DSAKLELKIP DDARSNAWVS FDGKRRQQLS RGDSVRIYMS QHPLPTVNKS 970 980 DQTGDWFRSL IRCLNWNERL DQKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform." Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A. Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, CALMODULIN-BINDING DOMAIN.
[5]	"Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana." Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M. Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection." Chai M.-F., Chen Q.-J., An R., Chen Y.-M., Chen J., Wang X.-C. Plant Mol. Biol. 59:553-564(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007727 Genomic DNA. Translation: AAD41416.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE30130.1. AF337912 mRNA. Translation: AAG60064.1. AY099794 mRNA. Translation: AAM20645.1.
IPI	IPI00542614.
PIR	D86349.
RefSeq	NP_564145.1. NM_102013.4.
UniGene	At.28688. At.47967.
3D structure databases
ProteinModelPortal	Q9C5W3.
SMR	Q9C5W3. Positions 664-975.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G21640.1-P.
Proteomic databases
PaxDb	Q9C5W3.
PRIDE	Q9C5W3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G21640.1; AT1G21640.1; AT1G21640.
GeneID	838766.
KEGG	ath:AT1G21640.
Organism-specific databases
TAIR	At1g21640.
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000029602.
InParanoid	Q9C5W3.
PhylomeDB	Q9C5W3.
ProtClustDB	PLN02727.
Gene expression databases
ArrayExpress	Q9C5W3.
Genevestigator	Q9C5W3.
GermOnline	AT1G21640. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADK2_ARATH

Accession

Primary (citable) accession number: Q9C5W3
Secondary accession number(s): Q9XI15

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program