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Q9C5W3

- NADK2_ARATH

UniProt

Q9C5W3 - NADK2_ARATH

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Protein

NAD kinase 2, chloroplastic

Gene

NADK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in chlorophyll synthesis and chloroplast protection against oxidative damage.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.

Kineticsi

Measured at pH 7.9 and 25 degrees Celsius for all experiments.

  1. KM=430 µM for NAD1 Publication
  2. KM=740 µM for ATP1 Publication

Vmax=14.3 µmol/h/mg enzyme with ATP as substrate1 Publication

pH dependencei

Optimum pH is 7.9 at 25 degrees Celsius.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1835-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 2, chloroplastic (EC:2.7.1.23)
Short name:
AtNADK-2
Gene namesi
Name:NADK2
Ordered Locus Names:At1g21640
ORF Names:F8K7.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G21640.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6161ChloroplastSequence AnalysisAdd
BLAST
Chaini62 – 985924NAD kinase 2, chloroplasticPRO_0000233705Add
BLAST

Proteomic databases

PaxDbiQ9C5W3.
PRIDEiQ9C5W3.

Expressioni

Tissue specificityi

Expressed in leaves.1 Publication

Developmental stagei

Expressed during development from young seedlings to flowering plants.1 Publication

Gene expression databases

ExpressionAtlasiQ9C5W3. baseline and differential.
GenevestigatoriQ9C5W3.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G21640.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5W3.
SMRiQ9C5W3. Positions 664-975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni335 – 38046Calmodulin-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the NAD kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000029602.
InParanoidiQ9C5W3.
PhylomeDBiQ9C5W3.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
3.90.190.10. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
IPR029021. Prot-tyrosine_phosphatase-like.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF52799. SSF52799. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9C5W3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFLCFCPCHV PIMSRLSPAT GISSRLRFSI GLSSDGRLIP FGFRFRRNDV
60 70 80 90 100
PFKRRLRFVI RAQLSEAFSP DLGLDSQAVK SRDTSNLPWI GPVPGDIAEV
110 120 130 140 150
EAYCRIFRSA ERLHGALMET LCNPVTGECR VPYDFSPEEK PLLEDKIVSV
160 170 180 190 200
LGCILSLLNK GRKEILSGRS SSMNSFNLDD VGVAEESLPP LAVFRGEMKR
210 220 230 240 250
CCESLHIALE NYLTPDDERS GIVWRKLQKL KNVCYDAGFP RSDNYPCQTL
260 270 280 290 300
FANWDPIYSS NTKEDIDSYE SEIAFWRGGQ VTQEGLKWLI ENGFKTIVDL
310 320 330 340 350
RAEIVKDTFY QTALDDAISL GKITVVQIPI DVRMAPKAEQ VELFASIVSD
360 370 380 390 400
SSKRPIYVHS KEGVWRTSAM VSRWKQYMTR PITKEIPVSE ESKRREVSET
410 420 430 440 450
KLGSNAVVSG KGVPDEQTDK VSEINEVDSR SASSQSKESG RFEGDTSASE
460 470 480 490 500
FNMVSDPLKS QVPPGNIFSR KEMSKFLKSK SIAPAGYLTN PSKILGTVPT
510 520 530 540 550
PQFSYTGVTN GNQIVDKDSI RRLAETGNSN GTLLPTSSQS LDFGNGKFSN
560 570 580 590 600
GNVHASDNTN KSISDNRGNG FSAAPIAVPP SDNLSRAVGS HSVRESQTQR
610 620 630 640 650
NNSGSSSDSS DDEAGAIEGN MCASATGVVR VQSRKKAEMF LVRTDGVSCT
660 670 680 690 700
REKVTESSLA FTHPSTQQQM LLWKTTPKTV LLLKKLGQEL MEEAKEAASF
710 720 730 740 750
LYHQENMNVL VEPEVHDVFA RIPGFGFVQT FYIQDTSDLH ERVDFVACLG
760 770 780 790 800
GDGVILHASN LFKGAVPPVV SFNLGSLGFL TSHPFEDFRQ DLKRVIHGNN
810 820 830 840 850
TLDGVYITLR MRLRCEIYRK GKAMPGKVFD VLNEIVVDRG SNPYLSKIEC
860 870 880 890 900
YEHDRLITKV QGDGVIVATP TGSTAYSTAA GGSMVHPNVP CMLFTPICPH
910 920 930 940 950
SLSFRPVILP DSAKLELKIP DDARSNAWVS FDGKRRQQLS RGDSVRIYMS
960 970 980
QHPLPTVNKS DQTGDWFRSL IRCLNWNERL DQKAL
Length:985
Mass (Da):109,188
Last modified:June 1, 2001 - v1
Checksum:iCF9BDFD804AF5C0E
GO

Sequence cautioni

The sequence AAD41416.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007727 Genomic DNA. Translation: AAD41416.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30130.1.
AF337912 mRNA. Translation: AAG60064.1.
AY099794 mRNA. Translation: AAM20645.1.
PIRiD86349.
RefSeqiNP_564145.1. NM_102013.4. [Q9C5W3-1]
UniGeneiAt.28688.
At.47967.

Genome annotation databases

EnsemblPlantsiAT1G21640.1; AT1G21640.1; AT1G21640. [Q9C5W3-1]
GeneIDi838766.
KEGGiath:AT1G21640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007727 Genomic DNA. Translation: AAD41416.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE30130.1 .
AF337912 mRNA. Translation: AAG60064.1 .
AY099794 mRNA. Translation: AAM20645.1 .
PIRi D86349.
RefSeqi NP_564145.1. NM_102013.4. [Q9C5W3-1 ]
UniGenei At.28688.
At.47967.

3D structure databases

ProteinModelPortali Q9C5W3.
SMRi Q9C5W3. Positions 664-975.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G21640.1-P.

Proteomic databases

PaxDbi Q9C5W3.
PRIDEi Q9C5W3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G21640.1 ; AT1G21640.1 ; AT1G21640 . [Q9C5W3-1 ]
GeneIDi 838766.
KEGGi ath:AT1G21640.

Organism-specific databases

TAIRi AT1G21640.

Phylogenomic databases

eggNOGi COG0061.
HOGENOMi HOG000029602.
InParanoidi Q9C5W3.
PhylomeDBi Q9C5W3.

Enzyme and pathway databases

BioCyci ARA:GQT-1835-MONOMER.

Miscellaneous databases

PROi Q9C5W3.

Gene expression databases

ExpressionAtlasi Q9C5W3. baseline and differential.
Genevestigatori Q9C5W3.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
3.90.190.10. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
IPR029021. Prot-tyrosine_phosphatase-like.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
SSF52799. SSF52799. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform."
    Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A.
    Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, CALMODULIN-BINDING DOMAIN.
  5. "Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana."
    Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M.
    Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection."
    Chai M.-F., Chen Q.-J., An R., Chen Y.-M., Chen J., Wang X.-C.
    Plant Mol. Biol. 59:553-564(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNADK2_ARATH
AccessioniPrimary (citable) accession number: Q9C5W3
Secondary accession number(s): Q9XI15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3