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Q9C5U8

- HIS8_ARATH

UniProt

Q9C5U8 - HIS8_ARATH

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Protein
Histidinol dehydrogenase, chloroplastic
Gene
HISN8, HDH, At5g63890, MGI19.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine By similarity.UniRule annotation

Catalytic activityi

L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei155 – 1551NAD By similarity
Binding sitei217 – 2171NAD By similarity
Binding sitei240 – 2401NAD By similarity
Binding sitei266 – 2661Substrate By similarity
Metal bindingi288 – 2881Zinc By similarity
Binding sitei288 – 2881Substrate By similarity
Metal bindingi291 – 2911Zinc By similarity
Binding sitei291 – 2911Substrate By similarity
Active sitei356 – 3561Proton acceptor By similarity
Active sitei357 – 3571Proton acceptor By similarity
Binding sitei357 – 3571Substrate By similarity
Metal bindingi390 – 3901Zinc By similarity
Binding sitei390 – 3901Substrate By similarity
Binding sitei444 – 4441Substrate By similarity
Metal bindingi449 – 4491Zinc By similarity
Binding sitei449 – 4491Substrate By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. histidinol dehydrogenase activity Source: TAIR
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. histidine biosynthetic process Source: UniProtKB-UniPathway
  2. pollen development Source: TAIR
  3. response to UV Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciARA:GQT-2426-MONOMER.
UniPathwayiUPA00031; UER00014.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol dehydrogenase, chloroplastic (EC:1.1.1.23)
Short name:
HDH
Alternative name(s):
Protein HISTIDINE BIOSYNTHESIS 8
Gene namesi
Name:HISN8
Synonyms:HDH
Ordered Locus Names:At5g63890
ORF Names:MGI19.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G63890.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030Chloroplast By similarity
Add
BLAST
Chaini31 – 466436Histidinol dehydrogenase, chloroplasticUniRule annotation
PRO_0000007215Add
BLAST

Proteomic databases

PaxDbiQ9C5U8.
PRIDEiQ9C5U8.

Expressioni

Gene expression databases

GenevestigatoriQ9C5U8.

Interactioni

Protein-protein interaction databases

BioGridi21751. 1 interaction.
STRINGi3702.AT5G63890.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5U8.
SMRiQ9C5U8. Positions 41-463.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
InParanoidiQ9C5U8.
KOiK00013.
OMAiYAAKLCG.
PhylomeDBiQ9C5U8.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9C5U8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLNLSRLSL LSSPRISIST HAPRKGYVCC SMKSYRLSEL SSSQVDSLKS    50
RPRIDFSSIF ATVNPIIDAV RSNGDNAVKE YTERFDKVQL NKVVEDMSEL 100
SVPELDSNVK EAFDVAYDNI YAFHLAQKST EKSVENMKGV RCKRVSRSIG 150
SVGLYVPGGT AVLPSTALML AIPAQIAGCK TVVLATPPSK DGSICKEVLY 200
CAKRAGVTHI LKAGGAQAIA AMAWGTDSCP KVEKIFGPGN QYVTAAKMIL 250
QNSEAMVSID MPAGPSEVLV IADEHASPVY IAADLLSQAE HGPDSQVVLV 300
VVGDSVDLNA IEEEIAKQCK SLPRGEFASK ALSHSFTVFA RDMIEAISFS 350
NLYAPEHLII NVKDAEKWEG LIENAGSVFI GPWTPESVGD YASGTNHVLP 400
TYGYARMYSG VSLDSFLKFM TVQSLTEEGL RNLGPYVATM AEIEGLDAHK 450
RAVTLRLKDI EAKQLA 466
Length:466
Mass (Da):50,323
Last modified:June 1, 2001 - v1
Checksum:i1175DEA979244595
GO
Isoform 2 (identifier: Q9C5U8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MSLNLSRLSLLSSPRISISTHAPRK → MNEFVDQLRFT

Note: No experimental confirmation available.

Show »
Length:452
Mass (Da):48,957
Checksum:i7DC0EAB6B02866F9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2525MSLNL…HAPRK → MNEFVDQLRFT in isoform 2.
VSP_047341Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251K → KA in BAB11037. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB027709 mRNA. Translation: BAB40445.1.
AB007646 Genomic DNA. Translation: BAB11037.1.
CP002688 Genomic DNA. Translation: AED97811.1.
CP002688 Genomic DNA. Translation: AED97812.1.
AY039881 mRNA. Translation: AAK63985.1.
AY143900 mRNA. Translation: AAN28839.1.
AY087987 mRNA. Translation: AAM65533.1.
RefSeqiNP_568981.2. NM_125784.3. [Q9C5U8-1]
NP_851260.1. NM_180929.3. [Q9C5U8-2]
UniGeneiAt.20645.
At.66745.

Genome annotation databases

EnsemblPlantsiAT5G63890.2; AT5G63890.2; AT5G63890. [Q9C5U8-1]
GeneIDi836509.
KEGGiath:AT5G63890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB027709 mRNA. Translation: BAB40445.1 .
AB007646 Genomic DNA. Translation: BAB11037.1 .
CP002688 Genomic DNA. Translation: AED97811.1 .
CP002688 Genomic DNA. Translation: AED97812.1 .
AY039881 mRNA. Translation: AAK63985.1 .
AY143900 mRNA. Translation: AAN28839.1 .
AY087987 mRNA. Translation: AAM65533.1 .
RefSeqi NP_568981.2. NM_125784.3. [Q9C5U8-1 ]
NP_851260.1. NM_180929.3. [Q9C5U8-2 ]
UniGenei At.20645.
At.66745.

3D structure databases

ProteinModelPortali Q9C5U8.
SMRi Q9C5U8. Positions 41-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21751. 1 interaction.
STRINGi 3702.AT5G63890.2-P.

Proteomic databases

PaxDbi Q9C5U8.
PRIDEi Q9C5U8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G63890.2 ; AT5G63890.2 ; AT5G63890 . [Q9C5U8-1 ]
GeneIDi 836509.
KEGGi ath:AT5G63890.

Organism-specific databases

TAIRi AT5G63890.

Phylogenomic databases

eggNOGi COG0141.
HOGENOMi HOG000243914.
InParanoidi Q9C5U8.
KOi K00013.
OMAi YAAKLCG.
PhylomeDBi Q9C5U8.

Enzyme and pathway databases

UniPathwayi UPA00031 ; UER00014 .
BioCyci ARA:GQT-2426-MONOMER.

Miscellaneous databases

PROi Q9C5U8.

Gene expression databases

Genevestigatori Q9C5U8.

Family and domain databases

HAMAPi MF_01024. HisD.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view ]
Pfami PF00815. Histidinol_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000099. Histidinol_dh. 1 hit.
PRINTSi PR00083. HOLDHDRGNASE.
SUPFAMi SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR00069. hisD. 1 hit.
PROSITEi PS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of Arabidopsis cDNA for histidinol dehydrogenase."
    Fujimori K.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Genetic dissection of histidine biosynthesis in Arabidopsis."
    Muralla R., Sweeney C., Stepansky A., Leustek T., Meinke D.
    Plant Physiol. 144:890-903(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiHIS8_ARATH
AccessioniPrimary (citable) accession number: Q9C5U8
Secondary accession number(s): F4KC60, Q8LA77, Q9FN00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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