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Protein

Histidinol dehydrogenase, chloroplastic

Gene

HISN8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.By similarity

Caution

Catalytic activityi

L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-histidine biosynthesis

This protein is involved in step 9 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase 1, chloroplastic (HISN1A), ATP phosphoribosyltransferase 2, chloroplastic (HISN1B)
  2. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  3. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (At2g36230), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic, 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (HISN3), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (AXX17_At2g32880)
  5. Imidazole glycerol phosphate synthase hisHF (AXX17_At4g31050), Imidazole glycerol phosphate synthase hisHF, chloroplastic (HISN4)
  6. Imidazoleglycerol-phosphate dehydratase 2, chloroplastic (HISN5B), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At3g24180), Imidazoleglycerol-phosphate dehydratase (At3g22425), Imidazoleglycerol-phosphate dehydratase 1, chloroplastic (HISN5A), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260)
  7. Histidinol-phosphate aminotransferase 2, chloroplastic (HISN6B), Histidinol-phosphate aminotransferase 1, chloroplastic (HISN6A)
  8. Bifunctional phosphatase IMPL2, chloroplastic (HISN7)
  9. Histidinol dehydrogenase, chloroplastic (HISN8), Histidinol dehydrogenase, chloroplastic (AXX17_At5g63470)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155NADBy similarity1
Binding sitei217NADBy similarity1
Binding sitei240NADBy similarity1
Binding sitei266SubstrateBy similarity1
Metal bindingi288ZincBy similarity1
Binding sitei288SubstrateBy similarity1
Metal bindingi291ZincBy similarity1
Binding sitei291SubstrateBy similarity1
Active sitei356Proton acceptorBy similarity1
Active sitei357Proton acceptorBy similarity1
Binding sitei357SubstrateBy similarity1
Metal bindingi390ZincBy similarity1
Binding sitei390SubstrateBy similarity1
Binding sitei444SubstrateBy similarity1
Metal bindingi449ZincBy similarity1
Binding sitei449SubstrateBy similarity1

GO - Molecular functioni

  • histidinol dehydrogenase activity Source: TAIR
  • NAD binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

  • histidine biosynthetic process Source: GO_Central
  • pollen development Source: TAIR
  • response to UV Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

UniPathwayiUPA00031; UER00014

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol dehydrogenase, chloroplastic (EC:1.1.1.23)
Short name:
HDH
Alternative name(s):
Protein HISTIDINE BIOSYNTHESIS 8
Gene namesi
Name:HISN8
Synonyms:HDH
Ordered Locus Names:At5g63890
ORF Names:MGI19.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G63890
TAIRilocus:2163946 AT5G63890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30ChloroplastBy similarityAdd BLAST30
ChainiPRO_000000721531 – 466Histidinol dehydrogenase, chloroplasticAdd BLAST436

Proteomic databases

PaxDbiQ9C5U8
PRIDEiQ9C5U8

PTM databases

iPTMnetiQ9C5U8

Expressioni

Gene expression databases

ExpressionAtlasiQ9C5U8 baseline and differential
GenevisibleiQ9C5U8 AT

Interactioni

Protein-protein interaction databases

BioGridi217511 interactor.
STRINGi3702.AT5G63890.2

Structurei

3D structure databases

ProteinModelPortaliQ9C5U8
SMRiQ9C5U8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidinol dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2697 Eukaryota
COG0141 LUCA
HOGENOMiHOG000243914
InParanoidiQ9C5U8
KOiK00013
OMAiQAEHDPM
OrthoDBiEOG09360BMC
PhylomeDBiQ9C5U8

Family and domain databases

CDDicd06572 Histidinol_dh, 1 hit
HAMAPiMF_01024 HisD, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR001692 Histidinol_DH_CS
IPR022695 Histidinol_DH_monofunct
IPR012131 Hstdl_DH
PANTHERiPTHR21256 PTHR21256, 1 hit
PfamiView protein in Pfam
PF00815 Histidinol_dh, 1 hit
PIRSFiPIRSF000099 Histidinol_dh, 1 hit
PRINTSiPR00083 HOLDHDRGNASE
SUPFAMiSSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR00069 hisD, 1 hit
PROSITEiView protein in PROSITE
PS00611 HISOL_DEHYDROGENASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C5U8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLNLSRLSL LSSPRISIST HAPRKGYVCC SMKSYRLSEL SSSQVDSLKS
60 70 80 90 100
RPRIDFSSIF ATVNPIIDAV RSNGDNAVKE YTERFDKVQL NKVVEDMSEL
110 120 130 140 150
SVPELDSNVK EAFDVAYDNI YAFHLAQKST EKSVENMKGV RCKRVSRSIG
160 170 180 190 200
SVGLYVPGGT AVLPSTALML AIPAQIAGCK TVVLATPPSK DGSICKEVLY
210 220 230 240 250
CAKRAGVTHI LKAGGAQAIA AMAWGTDSCP KVEKIFGPGN QYVTAAKMIL
260 270 280 290 300
QNSEAMVSID MPAGPSEVLV IADEHASPVY IAADLLSQAE HGPDSQVVLV
310 320 330 340 350
VVGDSVDLNA IEEEIAKQCK SLPRGEFASK ALSHSFTVFA RDMIEAISFS
360 370 380 390 400
NLYAPEHLII NVKDAEKWEG LIENAGSVFI GPWTPESVGD YASGTNHVLP
410 420 430 440 450
TYGYARMYSG VSLDSFLKFM TVQSLTEEGL RNLGPYVATM AEIEGLDAHK
460
RAVTLRLKDI EAKQLA
Length:466
Mass (Da):50,323
Last modified:June 1, 2001 - v1
Checksum:i1175DEA979244595
GO
Isoform 2 (identifier: Q9C5U8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MSLNLSRLSLLSSPRISISTHAPRK → MNEFVDQLRFT

Note: No experimental confirmation available.
Show »
Length:452
Mass (Da):48,957
Checksum:i7DC0EAB6B02866F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25K → KA in BAB11037 (PubMed:9501997).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0473411 – 25MSLNL…HAPRK → MNEFVDQLRFT in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027709 mRNA Translation: BAB40445.1
AB007646 Genomic DNA Translation: BAB11037.1
CP002688 Genomic DNA Translation: AED97811.1
CP002688 Genomic DNA Translation: AED97812.1
AY039881 mRNA Translation: AAK63985.1
AY143900 mRNA Translation: AAN28839.1
AY087987 mRNA Translation: AAM65533.1
RefSeqiNP_568981.2, NM_125784.3 [Q9C5U8-1]
NP_851260.1, NM_180929.4 [Q9C5U8-2]
UniGeneiAt.20645
At.66745

Genome annotation databases

EnsemblPlantsiAT5G63890.1; AT5G63890.1; AT5G63890 [Q9C5U8-2]
AT5G63890.2; AT5G63890.2; AT5G63890 [Q9C5U8-1]
GeneIDi836509
GrameneiAT5G63890.1; AT5G63890.1; AT5G63890 [Q9C5U8-2]
AT5G63890.2; AT5G63890.2; AT5G63890 [Q9C5U8-1]
KEGGiath:AT5G63890

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHIS8_ARATH
AccessioniPrimary (citable) accession number: Q9C5U8
Secondary accession number(s): F4KC60, Q8LA77, Q9FN00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome