Reviewed, UniProtKB/Swiss-Prot Q9C5U8 (HISX_ARATH)

Names and origin

Protein names

Recommended name:
    Histidinol dehydrogenase, chloroplastic
      Short name=HDH
    EC=1.1.1.23

Gene names

Name:	HDH
Ordered Locus Names:	At5g63890
ORF Names:	MGI19.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine By similarity.
Catalytic activity	L-histidinol + 2 NAD+ = L-histidine + 2 NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the histidinol dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pollen development Inferred from mutant phenotype. Source: TAIR response to UV Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro histidinol dehydrogenase activity Traceable author statement. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C5U8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9C5U8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Chloroplast By similarity
	Chain	31 – 466	436	Histidinol dehydrogenase, chloroplastic		PRO_0000007215
Sites
	Active site	356	1	Proton acceptor By similarity
	Active site	357	1	Proton acceptor By similarity
	Metal binding	288	1	Zinc By similarity
	Metal binding	291	1	Zinc By similarity
	Metal binding	390	1	Zinc By similarity
	Metal binding	449	1	Zinc By similarity
	Binding site	155	1	NAD By similarity
	Binding site	217	1	NAD By similarity
	Binding site	240	1	NAD By similarity
	Binding site	266	1	Substrate By similarity
	Binding site	288	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	357	1	Substrate By similarity
	Binding site	390	1	Substrate By similarity
	Binding site	444	1	Substrate By similarity
	Binding site	449	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 31	31	Missing in isoform 2.		VSP_008912
Experimental info
	Sequence conflict	25	1	K → KA in BAB11037. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 1175DEA979244595	FASTA	466	50,323
	10 20 30 40 50 60 MSLNLSRLSL LSSPRISIST HAPRKGYVCC SMKSYRLSEL SSSQVDSLKS RPRIDFSSIF 70 80 90 100 110 120 ATVNPIIDAV RSNGDNAVKE YTERFDKVQL NKVVEDMSEL SVPELDSNVK EAFDVAYDNI 130 140 150 160 170 180 YAFHLAQKST EKSVENMKGV RCKRVSRSIG SVGLYVPGGT AVLPSTALML AIPAQIAGCK 190 200 210 220 230 240 TVVLATPPSK DGSICKEVLY CAKRAGVTHI LKAGGAQAIA AMAWGTDSCP KVEKIFGPGN 250 260 270 280 290 300 QYVTAAKMIL QNSEAMVSID MPAGPSEVLV IADEHASPVY IAADLLSQAE HGPDSQVVLV 310 320 330 340 350 360 VVGDSVDLNA IEEEIAKQCK SLPRGEFASK ALSHSFTVFA RDMIEAISFS NLYAPEHLII 370 380 390 400 410 420 NVKDAEKWEG LIENAGSVFI GPWTPESVGD YASGTNHVLP TYGYARMYSG VSLDSFLKFM 430 440 450 460 TVQSLTEEGL RNLGPYVATM AEIEGLDAHK RAVTLRLKDI EAKQLA
	Isoform 2. Checksum: 9B65E2EF94B96524	FASTA	435	46,963
	10 20 30 40 50 60 MKSYRLSELS SSQVDSLKSR PRIDFSSIFA TVNPIIDAVR SNGDNAVKEY TERFDKVQLN 70 80 90 100 110 120 KVVEDMSELS VPELDSNVKE AFDVAYDNIY AFHLAQKSTE KSVENMKGVR CKRVSRSIGS 130 140 150 160 170 180 VGLYVPGGTA VLPSTALMLA IPAQIAGCKT VVLATPPSKD GSICKEVLYC AKRAGVTHIL 190 200 210 220 230 240 KAGGAQAIAA MAWGTDSCPK VEKIFGPGNQ YVTAAKMILQ NSEAMVSIDM PAGPSEVLVI 250 260 270 280 290 300 ADEHASPVYI AADLLSQAEH GPDSQVVLVV VGDSVDLNAI EEEIAKQCKS LPRGEFASKA 310 320 330 340 350 360 LSHSFTVFAR DMIEAISFSN LYAPEHLIIN VKDAEKWEGL IENAGSVFIG PWTPESVGDY 370 380 390 400 410 420 ASGTNHVLPT YGYARMYSGV SLDSFLKFMT VQSLTEEGLR NLGPYVATMA EIEGLDAHKR 430 AVTLRLKDIE AKQLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of Arabidopsis cDNA for histidinol dehydrogenase." Fujimori K. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB027709 mRNA. Translation: BAB40445.1. AB007646 Genomic DNA. Translation: BAB11037.1. AY039881 mRNA. Translation: AAK63985.1. AY143900 mRNA. Translation: AAN28839.1. AY087987 mRNA. Translation: AAM65533.1.
IPI	IPI00536632. IPI00549107.
RefSeq	NP_568981.2.
UniGene	At.20645
3D structure databases
HSSP	HSSP built from PDB template 1K75 based on UniProtKB P06988.
ModBase	Search...
Proteomic databases
PRIDE	Q9C5U8.
Genome annotation databases
GeneID	836509.
GenomeReviews	Gene locus AT5G63890 in contig BA000015_GR.
KEGG	ath:AT5G63890.
NMPDR	fig|3702.1.peg.28443.
Organism-specific databases
TAIR	At5g63890.
Phylogenomic databases
OMA	Q9C5U8. LDAHKNA.
Enzyme and pathway databases
BRENDA	1.1.1.23. 302.
Gene expression databases
ArrayExpress	Q9C5U8.
GermOnline	AT5G63890. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001692. Histidinol_DH_CS. IPR012131. Hstdl_DH_prok-type. [Graphical view]
PANTHER	PTHR21256:SF2. Hstdl_DH_prok. 1 hit.
Pfam	PF00815. Histidinol_dh. 1 hit. [Graphical view]
PRINTS	PR00083. HOLDHDRGNASE.
ProDom	PD002680. Histidinol_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00069. hisD. 1 hit.
PROSITE	PS00611. HISOL_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HISX_ARATH

Accession

Primary (citable) accession number: Q9C5U8
Secondary accession number(s): Q8LA77, Q9FN00

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)