Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9C5U8

- HIS8_ARATH

UniProt

Q9C5U8 - HIS8_ARATH

Protein

Histidinol dehydrogenase, chloroplastic

Gene

HISN8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.By similarity

    Catalytic activityi

    L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei155 – 1551NADBy similarity
    Binding sitei217 – 2171NADBy similarity
    Binding sitei240 – 2401NADBy similarity
    Binding sitei266 – 2661SubstrateBy similarity
    Metal bindingi288 – 2881ZincBy similarity
    Binding sitei288 – 2881SubstrateBy similarity
    Metal bindingi291 – 2911ZincBy similarity
    Binding sitei291 – 2911SubstrateBy similarity
    Active sitei356 – 3561Proton acceptorBy similarity
    Active sitei357 – 3571Proton acceptorBy similarity
    Binding sitei357 – 3571SubstrateBy similarity
    Metal bindingi390 – 3901ZincBy similarity
    Binding sitei390 – 3901SubstrateBy similarity
    Binding sitei444 – 4441SubstrateBy similarity
    Metal bindingi449 – 4491ZincBy similarity
    Binding sitei449 – 4491SubstrateBy similarity

    GO - Molecular functioni

    1. histidinol dehydrogenase activity Source: TAIR
    2. NAD binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. histidine biosynthetic process Source: UniProtKB-UniPathway
    2. pollen development Source: TAIR
    3. response to UV Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciARA:GQT-2426-MONOMER.
    UniPathwayiUPA00031; UER00014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histidinol dehydrogenase, chloroplastic (EC:1.1.1.23)
    Short name:
    HDH
    Alternative name(s):
    Protein HISTIDINE BIOSYNTHESIS 8
    Gene namesi
    Name:HISN8
    Synonyms:HDH
    Ordered Locus Names:At5g63890
    ORF Names:MGI19.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G63890.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030ChloroplastBy similarityAdd
    BLAST
    Chaini31 – 466436Histidinol dehydrogenase, chloroplasticPRO_0000007215Add
    BLAST

    Proteomic databases

    PaxDbiQ9C5U8.
    PRIDEiQ9C5U8.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9C5U8.

    Interactioni

    Protein-protein interaction databases

    BioGridi21751. 1 interaction.
    STRINGi3702.AT5G63890.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5U8.
    SMRiQ9C5U8. Positions 41-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidinol dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0141.
    HOGENOMiHOG000243914.
    InParanoidiQ9C5U8.
    KOiK00013.
    OMAiYAAKLCG.
    PhylomeDBiQ9C5U8.

    Family and domain databases

    HAMAPiMF_01024. HisD.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR001692. Histidinol_DH_CS.
    IPR022695. Histidinol_DH_monofunct.
    IPR012131. Hstdl_DH.
    [Graphical view]
    PfamiPF00815. Histidinol_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
    PRINTSiPR00083. HOLDHDRGNASE.
    SUPFAMiSSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR00069. hisD. 1 hit.
    PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9C5U8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLNLSRLSL LSSPRISIST HAPRKGYVCC SMKSYRLSEL SSSQVDSLKS    50
    RPRIDFSSIF ATVNPIIDAV RSNGDNAVKE YTERFDKVQL NKVVEDMSEL 100
    SVPELDSNVK EAFDVAYDNI YAFHLAQKST EKSVENMKGV RCKRVSRSIG 150
    SVGLYVPGGT AVLPSTALML AIPAQIAGCK TVVLATPPSK DGSICKEVLY 200
    CAKRAGVTHI LKAGGAQAIA AMAWGTDSCP KVEKIFGPGN QYVTAAKMIL 250
    QNSEAMVSID MPAGPSEVLV IADEHASPVY IAADLLSQAE HGPDSQVVLV 300
    VVGDSVDLNA IEEEIAKQCK SLPRGEFASK ALSHSFTVFA RDMIEAISFS 350
    NLYAPEHLII NVKDAEKWEG LIENAGSVFI GPWTPESVGD YASGTNHVLP 400
    TYGYARMYSG VSLDSFLKFM TVQSLTEEGL RNLGPYVATM AEIEGLDAHK 450
    RAVTLRLKDI EAKQLA 466
    Length:466
    Mass (Da):50,323
    Last modified:June 1, 2001 - v1
    Checksum:i1175DEA979244595
    GO
    Isoform 2 (identifier: Q9C5U8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-25: MSLNLSRLSLLSSPRISISTHAPRK → MNEFVDQLRFT

    Note: No experimental confirmation available.

    Show »
    Length:452
    Mass (Da):48,957
    Checksum:i7DC0EAB6B02866F9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251K → KA in BAB11037. (PubMed:9501997)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2525MSLNL…HAPRK → MNEFVDQLRFT in isoform 2. 1 PublicationVSP_047341Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB027709 mRNA. Translation: BAB40445.1.
    AB007646 Genomic DNA. Translation: BAB11037.1.
    CP002688 Genomic DNA. Translation: AED97811.1.
    CP002688 Genomic DNA. Translation: AED97812.1.
    AY039881 mRNA. Translation: AAK63985.1.
    AY143900 mRNA. Translation: AAN28839.1.
    AY087987 mRNA. Translation: AAM65533.1.
    RefSeqiNP_568981.2. NM_125784.3. [Q9C5U8-1]
    NP_851260.1. NM_180929.3. [Q9C5U8-2]
    UniGeneiAt.20645.
    At.66745.

    Genome annotation databases

    EnsemblPlantsiAT5G63890.2; AT5G63890.2; AT5G63890. [Q9C5U8-1]
    GeneIDi836509.
    KEGGiath:AT5G63890.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB027709 mRNA. Translation: BAB40445.1 .
    AB007646 Genomic DNA. Translation: BAB11037.1 .
    CP002688 Genomic DNA. Translation: AED97811.1 .
    CP002688 Genomic DNA. Translation: AED97812.1 .
    AY039881 mRNA. Translation: AAK63985.1 .
    AY143900 mRNA. Translation: AAN28839.1 .
    AY087987 mRNA. Translation: AAM65533.1 .
    RefSeqi NP_568981.2. NM_125784.3. [Q9C5U8-1 ]
    NP_851260.1. NM_180929.3. [Q9C5U8-2 ]
    UniGenei At.20645.
    At.66745.

    3D structure databases

    ProteinModelPortali Q9C5U8.
    SMRi Q9C5U8. Positions 41-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21751. 1 interaction.
    STRINGi 3702.AT5G63890.2-P.

    Proteomic databases

    PaxDbi Q9C5U8.
    PRIDEi Q9C5U8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G63890.2 ; AT5G63890.2 ; AT5G63890 . [Q9C5U8-1 ]
    GeneIDi 836509.
    KEGGi ath:AT5G63890.

    Organism-specific databases

    TAIRi AT5G63890.

    Phylogenomic databases

    eggNOGi COG0141.
    HOGENOMi HOG000243914.
    InParanoidi Q9C5U8.
    KOi K00013.
    OMAi YAAKLCG.
    PhylomeDBi Q9C5U8.

    Enzyme and pathway databases

    UniPathwayi UPA00031 ; UER00014 .
    BioCyci ARA:GQT-2426-MONOMER.

    Miscellaneous databases

    PROi Q9C5U8.

    Gene expression databases

    Genevestigatori Q9C5U8.

    Family and domain databases

    HAMAPi MF_01024. HisD.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR001692. Histidinol_DH_CS.
    IPR022695. Histidinol_DH_monofunct.
    IPR012131. Hstdl_DH.
    [Graphical view ]
    Pfami PF00815. Histidinol_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000099. Histidinol_dh. 1 hit.
    PRINTSi PR00083. HOLDHDRGNASE.
    SUPFAMi SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR00069. hisD. 1 hit.
    PROSITEi PS00611. HISOL_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of Arabidopsis cDNA for histidinol dehydrogenase."
      Fujimori K.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Genetic dissection of histidine biosynthesis in Arabidopsis."
      Muralla R., Sweeney C., Stepansky A., Leustek T., Meinke D.
      Plant Physiol. 144:890-903(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiHIS8_ARATH
    AccessioniPrimary (citable) accession number: Q9C5U8
    Secondary accession number(s): F4KC60, Q8LA77, Q9FN00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3