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Protein

26S protease regulatory subunit 8 homolog A

Gene

RPT6A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi202 – 2098ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 8 homolog A
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT6a
26S proteasome subunit 8 homolog A
Regulatory particle triple-A ATPase subunit 6a
Gene namesi
Name:RPT6A
Synonyms:SUG1
Ordered Locus Names:At5g19990
ORF Names:F28I16.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19990.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • cytosolic proteasome complex Source: GO_Central
  • nuclear proteasome complex Source: GO_Central
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • proteasome complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 41941826S protease regulatory subunit 8 homolog APRO_0000391487Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Cross-linki406 – 406Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9C5U3.
PRIDEiQ9C5U3.

Expressioni

Gene expression databases

GenevisibleiQ9C5U3. AT.

Interactioni

Subunit structurei

Component of the 19S regulatory particule (RP/PA700) base subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi17397. 11 interactions.
IntActiQ9C5U3. 9 interactions.
STRINGi3702.AT5G19990.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5U3.
SMRiQ9C5U3. Positions 38-409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0728. Eukaryota.
COG1222. LUCA.
HOGENOMiHOG000225143.
InParanoidiQ9C5U3.
KOiK03066.
OMAiRTIDTIS.
OrthoDBiEOG09360CE6.
PhylomeDBiQ9C5U3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C5U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVGVDSRR PETAMEETCN VKGAAAKQGE GLKQYYLQHI HELQRQLRQK
60 70 80 90 100
TNNLNRLEAQ RNELNSRVRM LREELQLLQE PGSYVGEVVK VMGKNKVLVK
110 120 130 140 150
VHPEGKYVVD IDKSIDITKI TPSTRVALRN DSYVLHLVLP SKVDPLVNLM
160 170 180 190 200
KVEKVPDSTY DMIGGLDQQI KEIKEVIELP IKHPELFESL GIAQPKGVLL
210 220 230 240 250
YGPPGTGKTL LARAVAHHTD CTFIRVSGSE LVQKYIGEGS RMVRELFVMA
260 270 280 290 300
REHAPSIIFM DEIDSIGSAR MESGSGNGDS EVQRTMLELL NQLDGFEASN
310 320 330 340 350
KIKVLMATNR IDILDQALLR PGRIDRKIEF PNPNEESRFD ILKIHSRKMN
360 370 380 390 400
LMRGIDLKKI AEKMNGASGA ELKAVCTEAG MFALRERRVH VTQEDFEMAV
410
AKVMKKDTEK NMSLRKLWK
Length:419
Mass (Da):47,248
Last modified:June 1, 2001 - v1
Checksum:i3186E05B7142EC8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123395 mRNA. Translation: AAF22526.1.
AB044348 mRNA. Translation: BAB40755.1.
AF296836 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92777.1.
AY065174 mRNA. Translation: AAL38350.1.
BT009668 mRNA. Translation: AAP78936.1.
RefSeqiNP_568389.1. NM_122006.3.
UniGeneiAt.10507.

Genome annotation databases

EnsemblPlantsiAT5G19990.1; AT5G19990.1; AT5G19990.
GeneIDi832121.
GrameneiAT5G19990.1; AT5G19990.1; AT5G19990.
KEGGiath:AT5G19990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123395 mRNA. Translation: AAF22526.1.
AB044348 mRNA. Translation: BAB40755.1.
AF296836 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92777.1.
AY065174 mRNA. Translation: AAL38350.1.
BT009668 mRNA. Translation: AAP78936.1.
RefSeqiNP_568389.1. NM_122006.3.
UniGeneiAt.10507.

3D structure databases

ProteinModelPortaliQ9C5U3.
SMRiQ9C5U3. Positions 38-409.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17397. 11 interactions.
IntActiQ9C5U3. 9 interactions.
STRINGi3702.AT5G19990.1.

Proteomic databases

PaxDbiQ9C5U3.
PRIDEiQ9C5U3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19990.1; AT5G19990.1; AT5G19990.
GeneIDi832121.
GrameneiAT5G19990.1; AT5G19990.1; AT5G19990.
KEGGiath:AT5G19990.

Organism-specific databases

TAIRiAT5G19990.

Phylogenomic databases

eggNOGiKOG0728. Eukaryota.
COG1222. LUCA.
HOGENOMiHOG000225143.
InParanoidiQ9C5U3.
KOiK03066.
OMAiRTIDTIS.
OrthoDBiEOG09360CE6.
PhylomeDBiQ9C5U3.

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ9C5U3.

Gene expression databases

GenevisibleiQ9C5U3. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRS8A_ARATH
AccessioniPrimary (citable) accession number: Q9C5U3
Secondary accession number(s): Q9SEI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.