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Protein

Histidine kinase 4

Gene

AHK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokinins (CK) receptor related to bacterial two-component regulators. Binds also the synthetic urea-type cytokinin thiadiazuron, a potent defoliant and herbicide. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feed phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. In the absence of cytokinin, removes phosphate from HPt proteins, decreasing the system phosphoload. Involved in meristems establishment in seedlings. Acts as a redundant negative regulator of drought and salt stress responses, and abscisic acid (ABA) signaling in a cytokinin-dependent manner. Required to set vascular asymmetric cell divisions that establish phloem and procambium cell lines. Redundant positive regulator of cytokinin signaling that regulates many development process including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Can interact with isoprenoid-type cytokinins trans-zeatin (tZ and tZR), isopentenyladenine (iP), and isopentenyladenosine (iPR), the meta hydroxylated derivative of benzyladenine m-topolin, buta-2,3-dienyladenine (HA-8), penta-2,3-dienyladenine (HA-1), 4-methyl-penta-2,3-dienyladenine (HA-10), 4-hydroxy-2-butynyladenine (RM1), 2-butynyladenine (RM6), and to a lower extent, with cis-zeatin (cZ), zeatin riboside and dihydrozeatin (DZ). Together with AHK3, involved in the cytokinin-dependent responses to Pi starvation and sucrose stresses. Required for the formation of auxin-transporting vascular tissues in the hypocotyl, and primary and lateral roots, but not in adventitious roots, thus leading to auxin basipetal transport that regulates root development and branching. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Prevents the uptake of sulfate by mediating cytokinin-dependent down-regulation of high-affinity sulfate transporters (e.g. SULTR1;1 and SULTR1;2) expression in roots. Together with AHK2, required for growth and reproduction promotion stimulated by the endophytic fungus Piriformospora indica in a trans-zeatin-dependent manner. Required to trigger the phytotoxic effect of the snapdragon (Antirrhinum majus) flowers volatile organic compound (VOC) methyl benzoate (MB). Plays a role in the cytokinin-mediated repression of the iron uptake pathway.28 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Enzyme regulationi

Activated by cytokinins to initiate phosphorelay signaling. This cytokinin-mediated activation is repressed by the trans-zeatin antagonists 6-(2-hydroxy-3-methylbenzylamino)purine (PI-55) and 6-(2,5-Dihydroxybenzylamino)purine (LGR-991).3 Publications

pH dependencei

Optimum pH to bind cytokinin is about 7-8.5 at 0 degrees Celsius.2 Publications

Temperature dependencei

Cytokinin-binding is more stable at 0 degrees Celsius than at 20 and 37 degrees Celsius.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei285Cytokinin1
Binding sitei307Cytokinin; via amide nitrogen1
Binding sitei317Cytokinin1

GO - Molecular functioni

  • cytokine binding Source: TAIR
  • cytokinin receptor activity Source: TAIR
  • enzyme binding Source: UniProtKB
  • phosphoprotein phosphatase activity Source: TAIR
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: TAIR
  • protein histidine kinase binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • transmembrane histidine kinase cytokinin receptor activity Source: UniProtKB

GO - Biological processi

  • carbohydrate homeostasis Source: TAIR
  • cellular response to phosphate starvation Source: UniProtKB
  • cellular response to sucrose stimulus Source: UniProtKB
  • cytokinin-activated signaling pathway Source: TAIR
  • defense response to bacterium Source: TAIR
  • embryonic root morphogenesis Source: TAIR
  • nucleoside metabolic process Source: InterPro
  • osmosensory signaling pathway Source: TAIR
  • phosphorelay signal transduction system Source: UniProtKB
  • protein phosphorylation Source: TAIR
  • regulation of meristem development Source: UniProtKB
  • regulation of seed germination Source: TAIR
  • regulation of shoot system development Source: TAIR
  • response to water deprivation Source: TAIR
  • sulfate transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Kinase, Protein phosphatase, Transferase

Keywords - Biological processi

Cytokinin signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine kinase 4 (EC:2.7.13.3)
Alternative name(s):
Arabidopsis histidine kinase 4
Short name:
AtHK4
Cytokinin receptor CYTOKININ RESPONSE 1
Short name:
AtCRE1
Short name:
Cytokinin receptor CRE1
Phosphoprotein phosphatase AHK4 (EC:3.1.3.16)
Protein AUTHENTIC HIS-KINASE 4
Protein ROOT AS IN WOL 1
Protein WOODEN LEG
Gene namesi
Name:AHK4
Synonyms:CRE1, RAW1, WOL
Ordered Locus Names:At2g01830
ORF Names:T23K3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01830.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Topological domaini146 – 429ExtracellularSequence analysisAdd BLAST284
Transmembranei430 – 450HelicalSequence analysisAdd BLAST21
Topological domaini451 – 1080CytoplasmicSequence analysisAdd BLAST630

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced sensitivity to cytokinin (mostly in shoots). Narrow vascular cylinder composed mainly of protoxylem cell files, with no apparent metaxylem or phloem. Hypersensitivity to ABA. Strong drought and salinity tolerance only in the presence of CK. Reduced cytokinin repression of several Pi starvation-responsive genes and increased sucrose sensitivity. More rapid germination, reduced requirement for light, and decreased far-red light sensitivity. Reduced sensitivity to N-isobutyl decanamide. Impaired benzyladenine (6-BA)-mediated repression of the iron uptake pathway. Impaired meristematic development in seedlings.12 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi225A → L: Loss of activity. 1 Publication1
Mutagenesisi227A → L: Loss of activity. 1 Publication1
Mutagenesisi273Y → E: Loss of activity. 1 Publication1
Mutagenesisi279M → A: No effect on activity. 1 Publication1
Mutagenesisi285D → A, E or R: Loss of activity. 1 Publication1
Mutagenesisi301T → I in wol-1; locked in the phosphoprotein phosphatase active form, retardation of the primary root growth with reduced cell number and exclusive xylem differentiation within the vascular tissue associated with abnormal vascular asymmetric cell divisions, impaired metaxylem and phloem differentiation, and reduced cytokinin-binding ability leading to impaired kinase activity and cyokinin-mediated activation. 6 Publications1
Mutagenesisi303P → V: Loss of activity. 1 Publication1
Mutagenesisi304F → A: Loss of activity. 1 Publication1
Mutagenesisi306L → A: Loss of activity. 1 Publication1
Mutagenesisi307L → A: Loss of activity. 1 Publication1
Mutagenesisi317T → A: No effect on activity. 1 Publication1
Mutagenesisi343G → L: Loss of activity. 1 Publication1
Mutagenesisi435G → C: Constitutively activated independently of cytokinin. 1 Publication1
Mutagenesisi436F → S: Constitutively activated independently of cytokinin. 1 Publication1
Mutagenesisi447M → T: Constitutively activated independently of cytokinin. 1 Publication1
Mutagenesisi459M → I in wol-3; retardation of the primary root growth, no production of lateral roots and enhanced formation of adventitious roots associated with impaired auxin basipetal transport. 1 Publication1
Mutagenesisi471V → A: Constitutively activated independently of cytokinin. 1 Publication1
Mutagenesisi482H → Q: Reduced phosphoprotein phosphatase activity. 2 Publications1
Mutagenesisi490G → D in cre1-1; impaired histidine-kinase receptor activity and reduced responses to cytokinins, including rapid cell proliferation and shoot formation in tissue culture, repression of sulfate uptake, retardation of the primary root growth, no production of lateral roots and enhanced formation of adventitious roots associated with impaired auxin basipetal transport, as well as reduced cell number within the vascular tissues in roots. 4 Publications1
Mutagenesisi493G → R in cre1-6; reduced sensitivity to cytokinin. 1 Publication1
Mutagenesisi494M → L: Constitutively activated independently of cytokinin. 1 Publication1
Mutagenesisi529L → F in wol-2/raw1; impaired metaxylem and phloem differentiation, and reduced sensitivity to cytokinins. 1 Publication1
Mutagenesisi529L → F in wol-2; retardation of the primary root growth with reduced cell number and exclusive xylem differentiation within the vascular tissue associated with abnormal vascular asymmetric cell divisions, and impaired cytokinin-binding ability. 1 Publication1
Mutagenesisi708F → L: No histidine kinase activity, but normal phosphoprotein phosphatase activity. 1 Publication1
Mutagenesisi996D → N: Cytokinin-mediated autophosphorylation but impaired phosphotransfer to an HPt, abolished phosphoprotein phosphatase activity. 2 Publications1
Mutagenesisi1008T → I in cre1-4; slightly reduced sensitivity to cytokinin, and impaired cytokinin repression of several Pi starvation-responses. 1 Publication1
Mutagenesisi1032A → T in cre1-9; impaired cytokinin repression of several Pi starvation-responses. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL6124.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003985891 – 1080Histidine kinase 4Add BLAST1080

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei482Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1
Modified residuei9964-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated predominantly on His residues. Activation probably requires a transfer of a phosphate group between a His in the transmitter domain and an Asp of the receiver domain.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C5U0.

PTM databases

iPTMnetiQ9C5U0.

Expressioni

Tissue specificityi

Mostly expressed in roots, specifically in the vascular cylinder and pericycle, and, to a lower extent, in leaves and flowers. Present in seedlings.4 Publications

Developmental stagei

Expressed specifically in the vasculature since the early stages of embryogenesis. At the globular stage of embryogenesis, detected in the four innermost cells, which are the precursors of the vascular tissue. During the heart, torpedo, and nearly mature stages, expressed in the procambium of the cotyledon shoulders, prospective hypocotyl, and embryonic root. In seedlings, mainly localized in meristematic tissues (e.g. shoot apical meristem SAM, root tips, and growing leaf and lateral root primordia), especially in vasculature. Present in all the vasculature and the shoot apical meristem (SAM) of the adult plant. In flowers, localized in carpels and developing ovules. In the root tips, expressed in the central cylinder.3 Publications

Inductioni

Rapidly induced by dehydration. Down-regulated by Pi starvation and induced by cytokinins.2 Publications

Gene expression databases

GenevisibleiQ9C5U0. AT.

Interactioni

Subunit structurei

Homodimer. Interacts with AHP1, AHP2, AHP3, AHP5, AHK3, AMPD, FBR12, WNK5 and At4g15630.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHK3Q9C5U12EBI-1100775,EBI-1100653
AMPDO804522EBI-1100775,EBI-1807679
At1g03430Q67XQ13EBI-1100775,EBI-1100725
At4g15630Q8L8Z12EBI-1100775,EBI-1807704
WNK5Q9SCU52EBI-1100775,EBI-1807651

GO - Molecular functioni

  • cytokine binding Source: TAIR
  • enzyme binding Source: UniProtKB
  • protein histidine kinase binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117. 32 interactors.
IntActiQ9C5U0. 29 interactors.
STRINGi3702.AT2G01830.2.

Chemistry databases

BindingDBiQ9C5U0.

Structurei

Secondary structure

11080
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi151 – 193Combined sources43
Turni194 – 196Combined sources3
Beta strandi197 – 199Combined sources3
Helixi204 – 213Combined sources10
Helixi215 – 217Combined sources3
Beta strandi221 – 228Combined sources8
Helixi232 – 234Combined sources3
Helixi235 – 242Combined sources8
Turni249 – 251Combined sources3
Beta strandi259 – 261Combined sources3
Beta strandi263 – 267Combined sources5
Helixi269 – 274Combined sources6
Helixi279 – 281Combined sources3
Helixi283 – 295Combined sources13
Turni307 – 309Combined sources3
Beta strandi312 – 321Combined sources10
Helixi331 – 336Combined sources6
Beta strandi338 – 346Combined sources9
Helixi348 – 357Combined sources10
Helixi362 – 364Combined sources3
Beta strandi365 – 371Combined sources7
Beta strandi379 – 382Combined sources4
Beta strandi385 – 387Combined sources3
Beta strandi395 – 399Combined sources5
Beta strandi408 – 414Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T4JX-ray1.65A/B149-418[»]
3T4KX-ray1.77A/B149-418[»]
3T4LX-ray1.53A/B149-418[»]
3T4OX-ray1.75A/B149-418[»]
3T4QX-ray2.30A/B149-418[»]
3T4SX-ray1.60A/B149-418[»]
3T4TX-ray1.70A/B149-418[»]
ProteinModelPortaliQ9C5U0.
SMRiQ9C5U0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9C5U0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 411CHASEPROSITE-ProRule annotationAdd BLAST214
Domaini479 – 760Histidine kinasePROSITE-ProRule annotationAdd BLAST282
Domaini786 – 920Response regulatory 1PROSITE-ProRule annotationAdd BLAST135
Domaini946 – 1071Response regulatory 2PROSITE-ProRule annotationAdd BLAST126

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi823 – 826Poly-Ala4

Sequence similaritiesi

Contains 1 CHASE domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 2 response regulatory domains.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0519. Eukaryota.
COG0642. LUCA.
InParanoidiQ9C5U0.
KOiK14489.
OMAiSENRMST.
OrthoDBiEOG093601CR.
PhylomeDBiQ9C5U0.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR006189. CHASE_dom.
IPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000836. PRibTrfase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF03924. CHASE. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM01079. CHASE. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 2 hits.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50839. CHASE. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C5U0-1) [UniParc]FASTAAdd to basket
Also known as: CRE1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRDFVYNNN AMFNPLTTHY SSDMNWALNN HQEEEEEPRR IEISDSESLE
60 70 80 90 100
NLKSSDFYQL GGGGALNSSE KPRKIDFWRS GLMGFAKMQQ QQQLQHSVAV
110 120 130 140 150
KMNNNNNNDL MGNKKGSTFI QEHRALLPKA LILWIIIVGF ISSGIYQWMD
160 170 180 190 200
DANKIRREEV LVSMCDQRAR MLQDQFSVSV NHVHALAILV STFHYHKNPS
210 220 230 240 250
AIDQETFAEY TARTAFERPL LSGVAYAEKV VNFEREMFER QHNWVIKTMD
260 270 280 290 300
RGEPSPVRDE YAPVIFSQDS VSYLESLDMM SGEEDRENIL RARETGKAVL
310 320 330 340 350
TSPFRLLETH HLGVVLTFPV YKSSLPENPT VEERIAATAG YLGGAFDVES
360 370 380 390 400
LVENLLGQLA GNQAIVVHVY DITNASDPLV MYGNQDEEAD RSLSHESKLD
410 420 430 440 450
FGDPFRKHKM ICRYHQKAPI PLNVLTTVPL FFAIGFLVGY ILYGAAMHIV
460 470 480 490 500
KVEDDFHEMQ ELKVRAEAAD VAKSQFLATV SHEIRTPMNG ILGMLAMLLD
510 520 530 540 550
TELSSTQRDY AQTAQVCGKA LIALINEVLD RAKIEAGKLE LESVPFDIRS
560 570 580 590 600
ILDDVLSLFS EESRNKSIEL AVFVSDKVPE IVKGDSGRFR QIIINLVGNS
610 620 630 640 650
VKFTEKGHIF VKVHLAEQSK DESEPKNALN GGVSEEMIVV SKQSSYNTLS
660 670 680 690 700
GYEAADGRNS WDSFKHLVSE EQSLSEFDIS SNVRLMVSIE DTGIGIPLVA
710 720 730 740 750
QGRVFMPFMQ ADSSTSRNYG GTGIGLSISK CLVELMRGQI NFISRPHIGS
760 770 780 790 800
TFWFTAVLEK CDKCSAINHM KKPNVEHLPS TFKGMKAIVV DAKPVRAAVT
810 820 830 840 850
RYHMKRLGIN VDVVTSLKTA VVAAAAFERN GSPLPTKPQL DMILVEKDSW
860 870 880 890 900
ISTEDNDSEI RLLNSRTNGN VHHKSPKLAL FATNITNSEF DRAKSAGFAD
910 920 930 940 950
TVIMKPLRAS MIGACLQQVL ELRKTRQQHP EGSSPATLKS LLTGKKILVV
960 970 980 990 1000
DDNIVNRRVA AGALKKFGAE VVCAESGQVA LGLLQIPHTF DACFMDIQMP
1010 1020 1030 1040 1050
QMDGFEATRQ IRMMEKETKE KTNLEWHLPI LAMTADVIHA TYEECLKSGM
1060 1070 1080
DGYVSKPFEE ENLYKSVAKS FKPNPISPSS
Length:1,080
Mass (Da):120,731
Last modified:June 1, 2001 - v1
Checksum:i5950DB968B529401
GO
Isoform 2 (identifier: Q9C5U0-2) [UniParc]FASTAAdd to basket
Also known as: CRE1a

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Show »
Length:1,057
Mass (Da):117,955
Checksum:iE1381C8685F0D787
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567S → G in BAB33311 (PubMed:11234017).Curated1
Sequence conflicti1018T → A in BAB33311 (PubMed:11234017).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti765S → N in strain: cv. Se-0. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0397701 – 23Missing in isoform 2. 3 PublicationsAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278528 mRNA. Translation: CAC18521.1.
AJ278529 mRNA. Translation: CAC18522.1.
AJ278530 mRNA. Translation: CAC18523.1.
AB049934 mRNA. Translation: BAB33310.1.
AB049935 mRNA. Translation: BAB33311.1.
AB046871 mRNA. Translation: BAB40776.1.
AC007069 Genomic DNA. Translation: AAD21777.2.
CP002685 Genomic DNA. Translation: AEC05505.1.
CP002685 Genomic DNA. Translation: AEC05506.1.
CP002685 Genomic DNA. Translation: AEC05507.1.
EF598292 Genomic DNA. Translation: ABQ85264.1.
EF598293 Genomic DNA. Translation: ABQ85265.1.
EF598294 Genomic DNA. Translation: ABQ85266.1.
EF598295 Genomic DNA. Translation: ABQ85267.1.
EF598296 Genomic DNA. Translation: ABQ85268.1.
EF598297 Genomic DNA. Translation: ABQ85269.1.
EF598298 Genomic DNA. Translation: ABQ85270.1.
EF598299 Genomic DNA. Translation: ABQ85271.1.
EF598300 Genomic DNA. Translation: ABQ85272.1.
EF598301 Genomic DNA. Translation: ABQ85273.1.
EF598302 Genomic DNA. Translation: ABQ85274.1.
EF598303 Genomic DNA. Translation: ABQ85275.1.
EF598304 Genomic DNA. Translation: ABQ85276.1.
EF598305 Genomic DNA. Translation: ABQ85277.1.
EF598306 Genomic DNA. Translation: ABQ85278.1.
EF598307 Genomic DNA. Translation: ABQ85279.1.
EF598308 Genomic DNA. Translation: ABQ85280.1.
EF598309 Genomic DNA. Translation: ABQ85281.1.
EF598310 Genomic DNA. Translation: ABQ85282.1.
EF598311 Genomic DNA. Translation: ABQ85283.1.
EF598312 Genomic DNA. Translation: ABQ85284.1.
EF598313 Genomic DNA. Translation: ABQ85285.1.
EF598314 Genomic DNA. Translation: ABQ85286.1.
EF598315 Genomic DNA. Translation: ABQ85287.1.
PIRiF84429.
RefSeqiNP_565277.1. NM_126244.3. [Q9C5U0-2]
NP_849925.1. NM_179594.2. [Q9C5U0-1]
NP_973396.1. NM_201667.1. [Q9C5U0-2]
UniGeneiAt.10485.

Genome annotation databases

EnsemblPlantsiAT2G01830.2; AT2G01830.2; AT2G01830. [Q9C5U0-1]
GeneIDi814714.
GrameneiAT2G01830.2; AT2G01830.2; AT2G01830.
KEGGiath:AT2G01830.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278528 mRNA. Translation: CAC18521.1.
AJ278529 mRNA. Translation: CAC18522.1.
AJ278530 mRNA. Translation: CAC18523.1.
AB049934 mRNA. Translation: BAB33310.1.
AB049935 mRNA. Translation: BAB33311.1.
AB046871 mRNA. Translation: BAB40776.1.
AC007069 Genomic DNA. Translation: AAD21777.2.
CP002685 Genomic DNA. Translation: AEC05505.1.
CP002685 Genomic DNA. Translation: AEC05506.1.
CP002685 Genomic DNA. Translation: AEC05507.1.
EF598292 Genomic DNA. Translation: ABQ85264.1.
EF598293 Genomic DNA. Translation: ABQ85265.1.
EF598294 Genomic DNA. Translation: ABQ85266.1.
EF598295 Genomic DNA. Translation: ABQ85267.1.
EF598296 Genomic DNA. Translation: ABQ85268.1.
EF598297 Genomic DNA. Translation: ABQ85269.1.
EF598298 Genomic DNA. Translation: ABQ85270.1.
EF598299 Genomic DNA. Translation: ABQ85271.1.
EF598300 Genomic DNA. Translation: ABQ85272.1.
EF598301 Genomic DNA. Translation: ABQ85273.1.
EF598302 Genomic DNA. Translation: ABQ85274.1.
EF598303 Genomic DNA. Translation: ABQ85275.1.
EF598304 Genomic DNA. Translation: ABQ85276.1.
EF598305 Genomic DNA. Translation: ABQ85277.1.
EF598306 Genomic DNA. Translation: ABQ85278.1.
EF598307 Genomic DNA. Translation: ABQ85279.1.
EF598308 Genomic DNA. Translation: ABQ85280.1.
EF598309 Genomic DNA. Translation: ABQ85281.1.
EF598310 Genomic DNA. Translation: ABQ85282.1.
EF598311 Genomic DNA. Translation: ABQ85283.1.
EF598312 Genomic DNA. Translation: ABQ85284.1.
EF598313 Genomic DNA. Translation: ABQ85285.1.
EF598314 Genomic DNA. Translation: ABQ85286.1.
EF598315 Genomic DNA. Translation: ABQ85287.1.
PIRiF84429.
RefSeqiNP_565277.1. NM_126244.3. [Q9C5U0-2]
NP_849925.1. NM_179594.2. [Q9C5U0-1]
NP_973396.1. NM_201667.1. [Q9C5U0-2]
UniGeneiAt.10485.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T4JX-ray1.65A/B149-418[»]
3T4KX-ray1.77A/B149-418[»]
3T4LX-ray1.53A/B149-418[»]
3T4OX-ray1.75A/B149-418[»]
3T4QX-ray2.30A/B149-418[»]
3T4SX-ray1.60A/B149-418[»]
3T4TX-ray1.70A/B149-418[»]
ProteinModelPortaliQ9C5U0.
SMRiQ9C5U0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117. 32 interactors.
IntActiQ9C5U0. 29 interactors.
STRINGi3702.AT2G01830.2.

Chemistry databases

BindingDBiQ9C5U0.
ChEMBLiCHEMBL6124.

PTM databases

iPTMnetiQ9C5U0.

Proteomic databases

PaxDbiQ9C5U0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01830.2; AT2G01830.2; AT2G01830. [Q9C5U0-1]
GeneIDi814714.
GrameneiAT2G01830.2; AT2G01830.2; AT2G01830.
KEGGiath:AT2G01830.

Organism-specific databases

TAIRiAT2G01830.

Phylogenomic databases

eggNOGiKOG0519. Eukaryota.
COG0642. LUCA.
InParanoidiQ9C5U0.
KOiK14489.
OMAiSENRMST.
OrthoDBiEOG093601CR.
PhylomeDBiQ9C5U0.

Miscellaneous databases

EvolutionaryTraceiQ9C5U0.
PROiQ9C5U0.

Gene expression databases

GenevisibleiQ9C5U0. AT.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR006189. CHASE_dom.
IPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000836. PRibTrfase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF03924. CHASE. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM01079. CHASE. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 2 hits.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50839. CHASE. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAHK4_ARATH
AccessioniPrimary (citable) accession number: Q9C5U0
Secondary accession number(s): A5YY60
, A5YY75, Q9C5T8, Q9C5T9, Q9FDZ3, Q9SIT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.