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Protein

WRKY transcription factor 18

Gene

WRKY18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positively modulates defense-related gene expression and disease resistance.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi170 – 23667WRKYPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • regulation of defense response Source: TAIR
  • response to chitin Source: TAIR
  • response to molecule of bacterial origin Source: TAIR
  • response to salicylic acid Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
WRKY transcription factor 18
Alternative name(s):
WRKY DNA-binding protein 18
Short name:
AtWRKY18
Gene namesi
Name:WRKY18
Ordered Locus Names:At4g31800
ORF Names:F28M20.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G31800.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310WRKY transcription factor 18PRO_0000133660Add
BLAST

Proteomic databases

PaxDbiQ9C5T4.
PRIDEiQ9C5T4.

PTM databases

iPTMnetiQ9C5T4.

Expressioni

Inductioni

By salicylic acid and pathogens.2 Publications

Gene expression databases

ExpressionAtlasiQ9C5T4. baseline and differential.
GenevisibleiQ9C5T4. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1993349,EBI-1993349
WRKY40Q9SAH77EBI-1993349,EBI-1993363
WRKY60Q9SK334EBI-1993349,EBI-2112777

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi14594. 5 interactions.
IntActiQ9C5T4. 4 interactions.
STRINGi3702.AT4G31800.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5T4.
SMRiQ9C5T4. Positions 175-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the WRKY group II-a family.Curated
Contains 1 WRKY DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410JCDQ. Eukaryota.
ENOG410YHES. LUCA.
HOGENOMiHOG000052516.
InParanoidiQ9C5T4.
OMAiECSSITE.
PhylomeDBiQ9C5T4.

Family and domain databases

Gene3Di2.20.25.80. 1 hit.
InterProiIPR003657. WRKY_dom.
[Graphical view]
PfamiPF03106. WRKY. 1 hit.
[Graphical view]
SMARTiSM00774. WRKY. 1 hit.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 1 hit.
PROSITEiPS50811. WRKY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9C5T4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGSSFLDIS LDLNTNPFSA KLPKKEVSVL ASTHLKRKWL EQDESASELR
60 70 80 90 100
EELNRVNSEN KKLTEMLARV CESYNELHNH LEKLQSRQSP EIEQTDIPIK
110 120 130 140 150
KRKQDPDEFL GFPIGLSSGK TENSSSNEDH HHHHQQHEQK NQLLSCKRPV
160 170 180 190 200
TDSFNKAKVS TVYVPTETSD TSLTVKDGFQ WRKYGQKVTR DNPSPRAYFR
210 220 230 240 250
CSFAPSCPVK KKVQRSAEDP SLLVATYEGT HNHLGPNASE GDATSQGGSS
260 270 280 290 300
TVTLDLVNGC HRLALEKNER DNTMQEVLIQ QMASSLTKDS KFTAALAAAI
310
SGRLMEQSRT
Length:310
Mass (Da):34,766
Last modified:May 15, 2002 - v2
Checksum:i762E6B520AC3FF8D
GO

Sequence cautioni

The sequence CAA19743.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79898.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti271 – 2711D → N (PubMed:11449049).Curated
Sequence conflicti271 – 2711D → N (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF224698 mRNA. Translation: AAK28308.1.
AL031004 Genomic DNA. Translation: CAA19743.1. Sequence problems.
AL161579 Genomic DNA. Translation: CAB79898.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85960.1.
AF428421 mRNA. Translation: AAL16190.1.
AY125557 mRNA. Translation: AAM78067.1.
AY088048 mRNA. Translation: AAM65594.1.
RefSeqiNP_567882.1. NM_119329.3. [Q9C5T4-1]
UniGeneiAt.19031.

Genome annotation databases

EnsemblPlantsiAT4G31800.1; AT4G31800.1; AT4G31800. [Q9C5T4-1]
GeneIDi829308.
KEGGiath:AT4G31800.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF224698 mRNA. Translation: AAK28308.1.
AL031004 Genomic DNA. Translation: CAA19743.1. Sequence problems.
AL161579 Genomic DNA. Translation: CAB79898.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85960.1.
AF428421 mRNA. Translation: AAL16190.1.
AY125557 mRNA. Translation: AAM78067.1.
AY088048 mRNA. Translation: AAM65594.1.
RefSeqiNP_567882.1. NM_119329.3. [Q9C5T4-1]
UniGeneiAt.19031.

3D structure databases

ProteinModelPortaliQ9C5T4.
SMRiQ9C5T4. Positions 175-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14594. 5 interactions.
IntActiQ9C5T4. 4 interactions.
STRINGi3702.AT4G31800.1.

PTM databases

iPTMnetiQ9C5T4.

Proteomic databases

PaxDbiQ9C5T4.
PRIDEiQ9C5T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G31800.1; AT4G31800.1; AT4G31800. [Q9C5T4-1]
GeneIDi829308.
KEGGiath:AT4G31800.

Organism-specific databases

TAIRiAT4G31800.

Phylogenomic databases

eggNOGiENOG410JCDQ. Eukaryota.
ENOG410YHES. LUCA.
HOGENOMiHOG000052516.
InParanoidiQ9C5T4.
OMAiECSSITE.
PhylomeDBiQ9C5T4.

Miscellaneous databases

PROiQ9C5T4.

Gene expression databases

ExpressionAtlasiQ9C5T4. baseline and differential.
GenevisibleiQ9C5T4. AT.

Family and domain databases

Gene3Di2.20.25.80. 1 hit.
InterProiIPR003657. WRKY_dom.
[Graphical view]
PfamiPF03106. WRKY. 1 hit.
[Graphical view]
SMARTiSM00774. WRKY. 1 hit.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 1 hit.
PROSITEiPS50811. WRKY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression."
    Yu D., Chen C., Chen Z.
    Plant Cell 13:1527-1540(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, INDUCTION.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor."
    Chen C., Chen Z.
    Plant Physiol. 129:706-716(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiWRK18_ARATH
AccessioniPrimary (citable) accession number: Q9C5T4
Secondary accession number(s): O81768, Q944H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: May 15, 2002
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Constitutive expression at high level causes severe abnormality in plant growth.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.