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Protein

2-Cys peroxiredoxin BAS1-like, chloroplastic

Gene

At5g06290

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf. Involved in the detoxification of alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system (By similarity).By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei126 – 1261Cysteine sulfenic acid (-SOH) intermediateBy similarity

GO - Molecular functioni

  • peroxidase activity Source: UniProtKB-KW
  • peroxiredoxin activity Source: TAIR

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • response to cold Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciARA:AT5G06290-MONOMER.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-5628897. TP53 Regulates Metabolic Genes.

Protein family/group databases

PeroxiBasei4361. At2CysPrx02.

Names & Taxonomyi

Protein namesi
Recommended name:
2-Cys peroxiredoxin BAS1-like, chloroplastic (EC:1.11.1.15)
Short name:
2-Cys Prx B
Short name:
2-Cys peroxiredoxin B
Alternative name(s):
Thiol-specific antioxidant protein B
Gene namesi
Ordered Locus Names:At5g06290
ORF Names:MHF15.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G06290.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • stromule Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7272ChloroplastBy similarityAdd
BLAST
Chaini73 – 2732012-Cys peroxiredoxin BAS1-like, chloroplasticPRO_0000284083Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi126 – 126Interchain (with C-248); in linked formBy similarity
Disulfide bondi248 – 248Interchain (with C-126); in linked formBy similarity

Post-translational modificationi

The Cys-126-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-126 (probably Cys-SOH) rapidly reacts with Cys-248-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin (By similarity).By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9C5R8.
PRIDEiQ9C5R8.

PTM databases

iPTMnetiQ9C5R8.

Expressioni

Inductioni

Down-regulated by ascorbate.1 Publication

Gene expression databases

GenevisibleiQ9C5R8. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation.By similarity

Protein-protein interaction databases

BioGridi15796. 3 interactions.
IntActiQ9C5R8. 3 interactions.
STRINGi3702.AT5G06290.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5R8.
SMRiQ9C5R8. Positions 82-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 239160ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AhpC/TSA family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
HOGENOMiHOG000022343.
InParanoidiQ9C5R8.
KOiK03386.
OMAiDHRVEEF.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C5R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMASIASSS STTLLSSSRV LLPSKSSLLS PTVSFPRIIP SSSASSSSLC
60 70 80 90 100
SGFSSLGSLT TNRSASRRNF AVKAQADDLP LVGNKAPDFE AEAVFDQEFI
110 120 130 140 150
KVKLSEYIGK KYVILFFYPL DFTFVCPTEI TAFSDRYEEF EKLNTEVLGV
160 170 180 190 200
SVDSVFSHLA WVQTDRKSGG LGDLNYPLVS DITKSISKSF GVLIPDQGIA
210 220 230 240 250
LRGLFIIDKE GVIQHSTINN LGIGRSVDET MRTLQALQYV QENPDEVCPA
260 270
GWKPGEKSMK PDPKLSKEYF SAI
Length:273
Mass (Da):29,780
Last modified:May 31, 2011 - v3
Checksum:i33CB87338DADEB1B
GO

Sequence cautioni

The sequence AAG40040.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB08951.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti217 – 2171T → P in AAG40040 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006700 Genomic DNA. Translation: BAB08951.1. Different initiation.
CP002688 Genomic DNA. Translation: AED90999.1.
AF324689 mRNA. Translation: AAG40040.2. Different initiation.
AF326871 mRNA. Translation: AAG41453.1.
AF339693 mRNA. Translation: AAK00375.1.
AY054621 mRNA. Translation: AAK96812.1.
AY081503 mRNA. Translation: AAM10065.1.
RefSeqiNP_568166.1. NM_120712.3.
UniGeneiAt.7277.

Genome annotation databases

EnsemblPlantsiAT5G06290.1; AT5G06290.1; AT5G06290.
GeneIDi830517.
GrameneiAT5G06290.1; AT5G06290.1; AT5G06290.
KEGGiath:AT5G06290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006700 Genomic DNA. Translation: BAB08951.1. Different initiation.
CP002688 Genomic DNA. Translation: AED90999.1.
AF324689 mRNA. Translation: AAG40040.2. Different initiation.
AF326871 mRNA. Translation: AAG41453.1.
AF339693 mRNA. Translation: AAK00375.1.
AY054621 mRNA. Translation: AAK96812.1.
AY081503 mRNA. Translation: AAM10065.1.
RefSeqiNP_568166.1. NM_120712.3.
UniGeneiAt.7277.

3D structure databases

ProteinModelPortaliQ9C5R8.
SMRiQ9C5R8. Positions 82-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15796. 3 interactions.
IntActiQ9C5R8. 3 interactions.
STRINGi3702.AT5G06290.1.

Protein family/group databases

PeroxiBasei4361. At2CysPrx02.

PTM databases

iPTMnetiQ9C5R8.

Proteomic databases

PaxDbiQ9C5R8.
PRIDEiQ9C5R8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G06290.1; AT5G06290.1; AT5G06290.
GeneIDi830517.
GrameneiAT5G06290.1; AT5G06290.1; AT5G06290.
KEGGiath:AT5G06290.

Organism-specific databases

TAIRiAT5G06290.

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
HOGENOMiHOG000022343.
InParanoidiQ9C5R8.
KOiK03386.
OMAiDHRVEEF.

Enzyme and pathway databases

BioCyciARA:AT5G06290-MONOMER.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-5628897. TP53 Regulates Metabolic Genes.

Miscellaneous databases

PROiQ9C5R8.

Gene expression databases

GenevisibleiQ9C5R8. AT.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
    Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Divergent light-, ascorbate-, and oxidative stress-dependent regulation of expression of the peroxiredoxin gene family in Arabidopsis."
    Horling F., Lamkemeyer P., Koenig J., Finkemeier I., Kandlbinder A., Baier M., Dietz K.-J.
    Plant Physiol. 131:317-325(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "The plant multigenic family of thiol peroxidases."
    Rouhier N., Jacquot J.-P.
    Free Radic. Biol. Med. 38:1413-1421(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.

Entry informationi

Entry nameiBAS1B_ARATH
AccessioniPrimary (citable) accession number: Q9C5R8
Secondary accession number(s): Q9FED5, Q9FNH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: May 31, 2011
Last modified: July 6, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.