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Protein

2-Cys peroxiredoxin BAS1-like, chloroplastic

Gene

At5g06290

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf.HMP:

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin CDSP32.HMP:

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.HMP:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei126Cysteine sulfenic acid (-SOH) intermediateHMP:1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • defense response to bacterium Source: TAIR
  • response to cold Source: TAIR

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciARA:AT5G06290-MONOMER.
ReactomeiR-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei4361. At2CysPrx02.

Names & Taxonomyi

Protein namesi
Recommended name:
2-Cys peroxiredoxin BAS1-like, chloroplastic (EC:1.11.1.15)
Short name:
2-Cys Prx B
Short name:
2-Cys peroxiredoxin B
Alternative name(s):
Thiol-specific antioxidant protein B
Gene namesi
Ordered Locus Names:At5g06290
ORF Names:MHF15.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G06290.
TAIRilocus:2164265. AT5G06290.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 72ChloroplastHMP:Add BLAST72
ChainiPRO_000028408373 – 2732-Cys peroxiredoxin BAS1-like, chloroplasticAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi126Interchain (with C-248); in linked formHMP:
Disulfide bondi248Interchain (with C-126); in linked formHMP:

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9C5R8.
PRIDEiQ9C5R8.

PTM databases

iPTMnetiQ9C5R8.

Expressioni

Inductioni

Down-regulated by ascorbate.1 Publication

Gene expression databases

ExpressionAtlasiQ9C5R8. baseline and differential.
GenevisibleiQ9C5R8. AT.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation (By similarity). Interacts with the plastidial thioredoxin CDSP32. Interacts with the plastidial NADPH-dependent thioredoxin reductase ANTR-C (By similarity).HMP:

Protein-protein interaction databases

BioGridi15796. 3 interactors.
IntActiQ9C5R8. 3 interactors.
STRINGi3702.AT5G06290.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5R8.
SMRiQ9C5R8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 239ThioredoxinIEP:Add BLAST160

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
HOGENOMiHOG000022343.
InParanoidiQ9C5R8.
KOiK03386.
OMAiCPANWEE.
OrthoDBiEOG09360MKG.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C5R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMASIASSS STTLLSSSRV LLPSKSSLLS PTVSFPRIIP SSSASSSSLC
60 70 80 90 100
SGFSSLGSLT TNRSASRRNF AVKAQADDLP LVGNKAPDFE AEAVFDQEFI
110 120 130 140 150
KVKLSEYIGK KYVILFFYPL DFTFVCPTEI TAFSDRYEEF EKLNTEVLGV
160 170 180 190 200
SVDSVFSHLA WVQTDRKSGG LGDLNYPLVS DITKSISKSF GVLIPDQGIA
210 220 230 240 250
LRGLFIIDKE GVIQHSTINN LGIGRSVDET MRTLQALQYV QENPDEVCPA
260 270
GWKPGEKSMK PDPKLSKEYF SAI
Length:273
Mass (Da):29,780
Last modified:May 31, 2011 - v3
Checksum:i33CB87338DADEB1B
GO

Sequence cautioni

The sequence AAG40040 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.IKR:
The sequence BAB08951 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.IKR:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti217T → P in AAG40040 (PubMed:14593172).IKR:1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006700 Genomic DNA. Translation: BAB08951.1. Different initiation.
CP002688 Genomic DNA. Translation: AED90999.1.
AF324689 mRNA. Translation: AAG40040.2. Different initiation.
AF326871 mRNA. Translation: AAG41453.1.
AF339693 mRNA. Translation: AAK00375.1.
AY054621 mRNA. Translation: AAK96812.1.
AY081503 mRNA. Translation: AAM10065.1.
RefSeqiNP_568166.1. NM_120712.4.
UniGeneiAt.7277.

Genome annotation databases

EnsemblPlantsiAT5G06290.1; AT5G06290.1; AT5G06290.
GeneIDi830517.
GrameneiAT5G06290.1; AT5G06290.1; AT5G06290.
KEGGiath:AT5G06290.

Similar proteinsi

Entry informationi

Entry nameiBAS1B_ARATH
AccessioniPrimary (citable) accession number: Q9C5R8
Secondary accession number(s): Q9FED5, Q9FNH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: May 31, 2011
Last modified: October 25, 2017
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families