Reviewed, UniProtKB/Swiss-Prot Q9C5P7 (SEC1A_ARATH)

Names and origin

Protein names

Recommended name:
    Protein transport Sec1a
      Short name=AtSec1a

Gene names

Name:	SEC1A
Ordered Locus Names:	At1g02010
ORF Names:	F22M8.14, T7I23.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the vesicle trafficking. Binds syntaxins By similarity.
Subunit structure	Does not bind the syntaxin KNOLLE.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.
Sequence caution	The sequence AAC24365.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAF76479.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein transport Transport
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking during exocytosis Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C5P7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Isoform 2 (identifier: Q9C5P7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     590-611: SDSVLKSASTEFKKLGQRIFVF → RHSILHNDFLFFGPFERSLDFF
     612-673: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9C5P7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     74-101: LVEELFKRREPMPGMDAIYFIQPSKENI → C
     150-160: Missing.
     452-461: Missing.
     590-606: SDSVLKSASTEFKKLGQ → RHSILHNDFLFFGPFE

Note: No experimental confirmation available. Incomplete sequence.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	Protein transport Sec1a		PRO_0000206295
Natural variations
	Alternative sequence	74 – 101	28	LVEEL…SKENI → C in isoform 3.		VSP_036635
	Alternative sequence	150 – 160	11	Missing in isoform 3.		VSP_036636
	Alternative sequence	452 – 461	10	Missing in isoform 3.		VSP_036637
	Alternative sequence	590 – 611	22	SDSVL…RIFVF → RHSILHNDFLFFGPFERSLD FF in isoform 2.		VSP_036638
	Alternative sequence	590 – 606	17	SDSVL…KKLGQ → RHSILHNDFLFFGPFE in isoform 3.		VSP_036639
	Alternative sequence	612 – 673	62	Missing in isoform 2.		VSP_036640
Experimental info
	Sequence conflict	102	1	V → F in BX816413. Ref.3
	Sequence conflict	610	1	V → L in BX816413. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 24, 2009. Version 3. Checksum: 34DD4C00816A75D7	FASTA	673	76,108
	10 20 30 40 50 60 MSFSDSESSS HGGGGGDYKF FRQISRDRLL HEMLGSTKTG DSKAWKILIM DRVTVKVMSQ 70 80 90 100 110 120 SCKMADITDQ GISLVEELFK RREPMPGMDA IYFIQPSKEN IVMFLSDMSG REPLYRKAFI 130 140 150 160 170 180 FFSSTIPKEL VNHIKSDSSV LPRIGALREM NMEYFPIDNQ GFLTDHEQAL ETLYAEDAEN 190 200 210 220 230 240 SRHFHICLNI MATRIATVFA SLKELPFVRY RAAKSTASRD LVPSKLAAAI WDCISKYKAI 250 260 270 280 290 300 PNFPQTETCE LLIVDRSVDQ IAPIIHEWTY DAMCHDLLDM EGNKHVIEVP SKTGGPPEKK 310 320 330 340 350 360 EIVLEDHDPV WLELRHTHIA DASERLHEKM TNFASKNKAA QMRSRDGSEL STRDLQKIVQ 370 380 390 400 410 420 ALPQYGEQVD KLSTHVELAG KINRIIRDTG LRDLGQLEQD LVFGDAGAKD VINFLRTNQD 430 440 450 460 470 480 TNPENKLRLL MIYATVYPEK FEGDKGVKLM QLARLSPVDM KVISNMQLIA GSPENKAKSG 490 500 510 520 530 540 SFSLKFDAGK TKQANRKDRS GEEETWQLFR FYPMIEELLE KLVKGDLSKS DYLCMNQSSH 550 560 570 580 590 600 KEESEARTGS VRKSSAPTAV PERKATPHSM RSRRTATWAR PHSSDDGYSS DSVLKSASTE 610 620 630 640 650 660 FKKLGQRIFV FIIGGATRSE LRVCHKLTSS LRREVVLGST SFDDPPQYIT KLKLLSEKDI 670 QGAPAQPFKP QYW
	Isoform 2. Checksum: 71127173E99D7067	FASTA	611	69,408
	10 20 30 40 50 60 MSFSDSESSS HGGGGGDYKF FRQISRDRLL HEMLGSTKTG DSKAWKILIM DRVTVKVMSQ 70 80 90 100 110 120 SCKMADITDQ GISLVEELFK RREPMPGMDA IYFIQPSKEN IVMFLSDMSG REPLYRKAFI 130 140 150 160 170 180 FFSSTIPKEL VNHIKSDSSV LPRIGALREM NMEYFPIDNQ GFLTDHEQAL ETLYAEDAEN 190 200 210 220 230 240 SRHFHICLNI MATRIATVFA SLKELPFVRY RAAKSTASRD LVPSKLAAAI WDCISKYKAI 250 260 270 280 290 300 PNFPQTETCE LLIVDRSVDQ IAPIIHEWTY DAMCHDLLDM EGNKHVIEVP SKTGGPPEKK 310 320 330 340 350 360 EIVLEDHDPV WLELRHTHIA DASERLHEKM TNFASKNKAA QMRSRDGSEL STRDLQKIVQ 370 380 390 400 410 420 ALPQYGEQVD KLSTHVELAG KINRIIRDTG LRDLGQLEQD LVFGDAGAKD VINFLRTNQD 430 440 450 460 470 480 TNPENKLRLL MIYATVYPEK FEGDKGVKLM QLARLSPVDM KVISNMQLIA GSPENKAKSG 490 500 510 520 530 540 SFSLKFDAGK TKQANRKDRS GEEETWQLFR FYPMIEELLE KLVKGDLSKS DYLCMNQSSH 550 560 570 580 590 600 KEESEARTGS VRKSSAPTAV PERKATPHSM RSRRTATWAR PHSSDDGYSR HSILHNDFLF 610 FGPFERSLDF F
	Isoform 3. Checksum: 783772D2B88596DD	FASTA	624	70,533
	10 20 30 40 50 60 MSFSDSESSS HGGGGGDYKF FRQISRDRLL HEMLGSTKTG DSKAWKILIM DRVTVKVMSQ 70 80 90 100 110 120 SCKMADITDQ GISCVMFLSD MSGREPLYRK AFIFFSSTIP KELVNHIKSD SSVLPRIGAL 130 140 150 160 170 180 REGFLTDHEQ ALETLYAEDA ENSRHFHICL NIMATRIATV FASLKELPFV RYRAAKSTAS 190 200 210 220 230 240 RDLVPSKLAA AIWDCISKYK AIPNFPQTET CELLIVDRSV DQIAPIIHEW TYDAMCHDLL 250 260 270 280 290 300 DMEGNKHVIE VPSKTGGPPE KKEIVLEDHD PVWLELRHTH IADASERLHE KMTNFASKNK 310 320 330 340 350 360 AAQMRSRDGS ELSTRDLQKI VQALPQYGEQ VDKLSTHVEL AGKINRIIRD TGLRDLGQLE 370 380 390 400 410 420 QDLVFGDAGA KDVINFLRTN QDTNPENKLR LLMIYATVYP EKFEGDKGVK LMQVISNMQL 430 440 450 460 470 480 IAGSPENKAK SGSFSLKFDA GKTKQANRKD RSGEEETWQL FRFYPMIEEL LEKLVKGDLS 490 500 510 520 530 540 KSDYLCMNQS SHKEESEART GSVRKSSAPT AVPERKATPH SMRSRRTATW ARPHSSDDGY 550 560 570 580 590 600 SRHSILHNDF LFFGPFERIF VFIIGGATRS ELRVCHKLTS SLRREVVLGS TSFDDPPQYI 610 620 TKLKLLSEKD IQGAPAQPFK PQYW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cytokinesis gene KEULE encodes a Sec1 protein that binds the syntaxin KNOLLE." Assaad F.F., Huet Y., Mayer U., Juergens G. J. Cell Biol. 152:531-544(2001) [PubMed: 11157980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Flower.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF335539 mRNA. Translation: AAK15767.1. AC020622 Genomic DNA. Translation: AAF76479.1. Sequence problems. U89959 Genomic DNA. Translation: AAC24365.1. Sequence problems. BX816413 mRNA. No translation available.
IPI	IPI00527087. IPI00923723. IPI00923751.
PIR	B86152.
RefSeq	NP_563643.4.
UniGene	At.42623
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9C5P7.
Genome annotation databases
GeneID	839288.
GenomeReviews	Gene locus AT1G02010 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g02010.
Phylogenomic databases
HOGENOM	HBG318312.
OMA	CHDLLEM.
Gene expression databases
Genevestigator	Q9C5P7.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SEC1A_ARATH

Accession

Primary (citable) accession number: Q9C5P7
Secondary accession number(s): O23672, Q9LPC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	March 24, 2009
Last modified:	December 15, 2009
This is version 51 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)