Q9C5P4 (SUVH3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH3
EC=2.1.1.43

Alternative name(s):
Histone H3-K9 methyltransferase 3
Short name=H3-K9-HMTase 3
Protein SET DOMAIN GROUP 19
Suppressor of variegation 3-9 homolog protein 3
Short name=Su(var)3-9 homolog protein 3

Gene names

Name:	SUVH3
Synonyms:	SDG19, SET19
Ordered Locus Names:	At1g73100
ORF Names:	F3N23.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus. Chromosome › centromere. Note: Associates with centromeric constitutive heterochromatin. Ref.1
Tissue specificity	Expressed in leaves stems and flowers. Ref.1
Domain	Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 A.T hook DNA-binding domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain. Contains 1 YDG domain.

Ontologies

Keywords
Cellular component	Centromere Chromosome Nucleus
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of gene expression, epigenetic Traceable author statement Ref.1. Source: TAIR
Cellular_component	chromosome Inferred from direct assay PubMed 16928780. Source: TAIR chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW histone methyltransferase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH3		PRO_0000186074
Regions
	Domain	208 – 355	148	YDG
	Domain	430 – 491	62	Pre-SET
	Domain	493 – 642	150	SET
	Domain	653 – 669	17	Post-SET
	DNA binding	108 – 120	13	A.T hook
Experimental info
	Sequence conflict	416	1	S → P in AAK28968. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9C5P4 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 8878E000747FD1AB	FASTA	669	73,439
	10 20 30 40 50 60 MQGVPGFNTV PNPNHYDKSI VLDIKPLRSL KPVFPNGNQG PPFVGCPPFG PSSSEYSSFF 70 80 90 100 110 120 PFGAQQPTHD TPDLNQTQNT PIPSFVPPLR SYRTPTKTNG PSSSSGTKRG VGRPKGTTSV 130 140 150 160 170 180 KKKEKKTVAN EPNLDVQVVK KFSSDFDSGI SAAEREDGNA YLVSSVLMRF DAVRRRLSQV 190 200 210 220 230 240 EFTKSATSKA AGTLMSNGVR TNMKKRVGTV PGIEVGDIFF SRIEMCLVGL HMQTMAGIDY 250 260 270 280 290 300 IISKAGSDEE SLATSIVSSG RYEGEAQDPE SLIYSGQGGN ADKNRQASDQ KLERGNLALE 310 320 330 340 350 360 NSLRKGNGVR VVRGEEDAAS KTGKIYIYDG LYSISESWVE KGKSGCNTFK YKLVRQPGQP 370 380 390 400 410 420 PAFGFWKSVQ KWKEGLTTRP GLILPDLTSG AESKPVSLVN DVDEDKGPAY FTYTSSLKYS 430 440 450 460 470 480 ETFKLTQPVI GCSCSGSCSP GNHNCSCIRK NDGDLPYLNG VILVSRRPVI YECGPTCPCH 490 500 510 520 530 540 ASCKNRVIQT GLKSRLEVFK TRNRGWGLRS WDSLRAGSFI CEYAGEVKDN GNLRGNQEED 550 560 570 580 590 600 AYVFDTSRVF NSFKWNYEPE LVDEDPSTEV PEEFNLPSPL LISAKKFGNV ARFMNHSCSP 610 620 630 640 650 660 NVFWQPVIRE GNGESVIHIA FFAMRHIPPM AELTYDYGIS PTSEARDESL LHGQRTCLCG SEQCRGSFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, NOMENCLATURE, TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Heterochromatin proteins and the control of heterochromatic gene silencing in Arabidopsis." Fischer A., Hofmann I., Naumann K., Reuter G. J. Plant Physiol. 163:358-368(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF344446 mRNA. Translation: AAK28968.1. AC008017 Genomic DNA. Translation: AAD55657.1. CP002684 Genomic DNA. Translation: AEE35414.1. AY099620 mRNA. Translation: AAM20471.1. BT002137 mRNA. Translation: AAN72148.1.
IPI	IPI00533947.
PIR	F96756.
RefSeq	NP_565056.1. NM_105968.3.
UniGene	At.11687. At.43232.
3D structure databases
ProteinModelPortal	Q9C5P4.
SMR	Q9C5P4. Positions 207-360, 386-666.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C5P4. 2 interactions.
STRING	3702.AT1G73100.1-P.
Proteomic databases
PaxDb	Q9C5P4.
PRIDE	Q9C5P4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G73100.1; AT1G73100.1; AT1G73100.
GeneID	843641.
KEGG	ath:AT1G73100.
Organism-specific databases
TAIR	At1g73100.
Phylogenomic databases
eggNOG	COG3440.
HOGENOM	HOG000238382.
InParanoid	Q9C5P4.
KO	K11420.
OMA	IHIAFFA.
PhylomeDB	Q9C5P4.
ProtClustDB	CLSN2917436.
Gene expression databases
Genevestigator	Q9C5P4.
GermOnline	AT1G73100. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.30.280.10. 1 hit.
InterPro	IPR025794. Hist-Lys_N-MeTrfase_plant. IPR003616. Post-SET_dom. IPR007728. Pre-SET_dom. IPR003606. Pre-SET_Zn-bd_sub. IPR001214. SET_dom. IPR003105. SRA_YDG. [Graphical view]
Pfam	PF05033. Pre-SET. 1 hit. PF00856. SET. 1 hit. PF02182. YDG_SRA. 1 hit. [Graphical view]
SMART	SM00508. PostSET. 1 hit. SM00468. PreSET. 1 hit. SM00317. SET. 1 hit. SM00466. SRA. 1 hit. [Graphical view]
PROSITE	PS50868. POST_SET. 1 hit. PS50867. PRE_SET. 1 hit. PS50280. SET. 1 hit. PS51015. YDG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUVH3_ARATH

Accession

Primary (citable) accession number: Q9C5P4
Secondary accession number(s): Q9SSL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	May 9, 2003
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program