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Q9C5P4 (SUVH3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH3

EC=2.1.1.43
Alternative name(s):
Histone H3-K9 methyltransferase 3
Short name=H3-K9-HMTase 3
Protein SET DOMAIN GROUP 19
Suppressor of variegation 3-9 homolog protein 3
Short name=Su(var)3-9 homolog protein 3
Gene names
Name:SUVH3
Synonyms:SDG19, SET19
Ordered Locus Names:At1g73100
ORF Names:F3N23.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length669 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location

Nucleus. Chromosomecentromere. Note: Associates with centromeric constitutive heterochromatin. Ref.1

Tissue specificity

Expressed in leaves stems and flowers. Ref.1

Domain

Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity.

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.

Contains 1 A.T hook DNA-binding domain.

Contains 1 post-SET domain.

Contains 1 pre-SET domain.

Contains 1 SET domain.

Contains 1 YDG domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 669669Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH3
PRO_0000186074

Regions

Domain208 – 355148YDG
Domain430 – 49162Pre-SET
Domain494 – 638145SET
Domain653 – 66917Post-SET
DNA binding108 – 12013A.T hook
Region504 – 5063S-adenosyl-L-methionine binding By similarity
Region595 – 5962S-adenosyl-L-methionine binding By similarity

Sites

Metal binding4321Zinc 1 By similarity
Metal binding4321Zinc 2 By similarity
Metal binding4341Zinc 1 By similarity
Metal binding4381Zinc 1 By similarity
Metal binding4381Zinc 3 By similarity
Metal binding4451Zinc 1 By similarity
Metal binding4471Zinc 2 By similarity
Metal binding4731Zinc 2 By similarity
Metal binding4731Zinc 3 By similarity
Metal binding4771Zinc 2 By similarity
Metal binding4791Zinc 3 By similarity
Metal binding4831Zinc 3 By similarity
Metal binding5981Zinc 4 By similarity
Metal binding6571Zinc 4 By similarity
Metal binding6591Zinc 4 By similarity
Metal binding6641Zinc 4 By similarity
Binding site5401S-adenosyl-L-methionine By similarity
Binding site5421S-adenosyl-L-methionine By similarity
Binding site5921S-adenosyl-L-methionine By similarity

Experimental info

Sequence conflict4161S → P in AAK28968. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9C5P4 [UniParc].

Last modified May 9, 2003. Version 2.
Checksum: 8878E000747FD1AB

FASTA66973,439
        10         20         30         40         50         60 
MQGVPGFNTV PNPNHYDKSI VLDIKPLRSL KPVFPNGNQG PPFVGCPPFG PSSSEYSSFF 

        70         80         90        100        110        120 
PFGAQQPTHD TPDLNQTQNT PIPSFVPPLR SYRTPTKTNG PSSSSGTKRG VGRPKGTTSV 

       130        140        150        160        170        180 
KKKEKKTVAN EPNLDVQVVK KFSSDFDSGI SAAEREDGNA YLVSSVLMRF DAVRRRLSQV 

       190        200        210        220        230        240 
EFTKSATSKA AGTLMSNGVR TNMKKRVGTV PGIEVGDIFF SRIEMCLVGL HMQTMAGIDY 

       250        260        270        280        290        300 
IISKAGSDEE SLATSIVSSG RYEGEAQDPE SLIYSGQGGN ADKNRQASDQ KLERGNLALE 

       310        320        330        340        350        360 
NSLRKGNGVR VVRGEEDAAS KTGKIYIYDG LYSISESWVE KGKSGCNTFK YKLVRQPGQP 

       370        380        390        400        410        420 
PAFGFWKSVQ KWKEGLTTRP GLILPDLTSG AESKPVSLVN DVDEDKGPAY FTYTSSLKYS 

       430        440        450        460        470        480 
ETFKLTQPVI GCSCSGSCSP GNHNCSCIRK NDGDLPYLNG VILVSRRPVI YECGPTCPCH 

       490        500        510        520        530        540 
ASCKNRVIQT GLKSRLEVFK TRNRGWGLRS WDSLRAGSFI CEYAGEVKDN GNLRGNQEED 

       550        560        570        580        590        600 
AYVFDTSRVF NSFKWNYEPE LVDEDPSTEV PEEFNLPSPL LISAKKFGNV ARFMNHSCSP 

       610        620        630        640        650        660 
NVFWQPVIRE GNGESVIHIA FFAMRHIPPM AELTYDYGIS PTSEARDESL LHGQRTCLCG 


SEQCRGSFG 

« Hide

References

« Hide 'large scale' references
[1]"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes."
Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B.
Nucleic Acids Res. 29:4319-4333(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, NOMENCLATURE, TISSUE SPECIFICITY.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Heterochromatin proteins and the control of heterochromatic gene silencing in Arabidopsis."
Fischer A., Hofmann I., Naumann K., Reuter G.
J. Plant Physiol. 163:358-368(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF344446 mRNA. Translation: AAK28968.1.
AC008017 Genomic DNA. Translation: AAD55657.1.
CP002684 Genomic DNA. Translation: AEE35414.1.
AY099620 mRNA. Translation: AAM20471.1.
BT002137 mRNA. Translation: AAN72148.1.
PIRF96756.
RefSeqNP_565056.1. NM_105968.3.
UniGeneAt.11687.
At.43232.

3D structure databases

ProteinModelPortalQ9C5P4.
SMRQ9C5P4. Positions 155-666.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid28860. 3 interactions.
IntActQ9C5P4. 2 interactions.
MINTMINT-8068307.
STRING3702.AT1G73100.1-P.

Proteomic databases

PaxDbQ9C5P4.
PRIDEQ9C5P4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G73100.1; AT1G73100.1; AT1G73100.
GeneID843641.
KEGGath:AT1G73100.

Organism-specific databases

TAIRAT1G73100.

Phylogenomic databases

eggNOGCOG3440.
HOGENOMHOG000238382.
InParanoidQ9C5P4.
KOK11420.
OMAIHIAFFA.
PhylomeDBQ9C5P4.

Enzyme and pathway databases

BioCycARA:AT1G73100-MONOMER.

Gene expression databases

GenevestigatorQ9C5P4.

Family and domain databases

Gene3D2.30.280.10. 1 hit.
InterProIPR025794. Hist-Lys_N-MeTrfase_plant.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR003606. Pre-SET_Zn-bd_sub.
IPR015947. PUA-like_domain.
IPR001214. SET_dom.
IPR003105. SRA_YDG.
[Graphical view]
PfamPF05033. Pre-SET. 1 hit.
PF02182. SAD_SRA. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00508. PostSET. 1 hit.
SM00468. PreSET. 1 hit.
SM00317. SET. 1 hit.
SM00466. SRA. 1 hit.
[Graphical view]
SUPFAMSSF88697. SSF88697. 1 hit.
PROSITEPS50868. POST_SET. 1 hit.
PS50867. PRE_SET. 1 hit.
PS51575. SAM_MT43_SUVAR39_2. 1 hit.
PS50280. SET. 1 hit.
PS51015. YDG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSUVH3_ARATH
AccessionPrimary (citable) accession number: Q9C5P4
Secondary accession number(s): Q9SSL7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 9, 2003
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names