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Protein

Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH8

Gene

SUVH8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression.

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei624S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei626S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei676S-adenosyl-L-methioninePROSITE-ProRule annotation1
Metal bindingi682ZincBy similarity1
Metal bindingi743ZincBy similarity1
Metal bindingi745ZincBy similarity1
Metal bindingi750ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi174 – 186A.T hookAdd BLAST13

GO - Molecular functioni

GO - Biological processi

  • leaf development Source: TAIR
  • regulation of cell size Source: TAIR
  • regulation of gene expression, epigenetic Source: TAIR

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Methyltransferase, Transferase
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH8 (EC:2.1.1.43)
Alternative name(s):
Histone H3-K9 methyltransferase 8
Short name:
H3-K9-HMTase 8
Protein SET DOMAIN GROUP 21
Suppressor of variegation 3-9 homolog protein 8
Short name:
Su(var)3-9 homolog protein 8
Gene namesi
Name:SUVH8
Synonyms:SDG21, SET21
Ordered Locus Names:At2g24740
ORF Names:F27A10.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G24740.
TAIRilocus:2047266. AT2G24740.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860791 – 755Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH8Add BLAST755

Proteomic databases

PaxDbiQ9C5P0.

PTM databases

iPTMnetiQ9C5P0.

Expressioni

Gene expression databases

GenevisibleiQ9C5P0. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT2G24740.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini310 – 448YDGPROSITE-ProRule annotationAdd BLAST139
Domaini528 – 578Pre-SETAdd BLAST51
Domaini581 – 723SETPROSITE-ProRule annotationAdd BLAST143
Domaini739 – 755Post-SETPROSITE-ProRule annotationAdd BLAST17

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni591 – 593S-adenosyl-L-methionine bindingBy similarity3
Regioni679 – 680S-adenosyl-L-methionine bindingBy similarity2

Domaini

Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity.
Cys residues in the pre-SET domain normally bind 3 zinc ions that are arranged in a triangular cluster, but here these Cys residues are only partially conserved, suggesting that the pre-Set domain may not bind a zinc cluster.

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1082. Eukaryota.
COG2940. LUCA.
COG3440. LUCA.
HOGENOMiHOG000238382.
InParanoidiQ9C5P0.
KOiK11420.
OMAiTASTNCM.
OrthoDBiEOG093613YB.
PhylomeDBiQ9C5P0.

Family and domain databases

Gene3Di2.30.280.10. 1 hit.
InterProiView protein in InterPro
IPR025794. Hist-Lys_N-MeTrfase_plant.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR015947. PUA-like_domain.
IPR001214. SET_dom.
IPR003105. SRA_YDG.
PfamiView protein in Pfam
PF05033. Pre-SET. 1 hit.
PF02182. SAD_SRA. 1 hit.
PF00856. SET. 1 hit.
SMARTiView protein in SMART
SM00468. PreSET. 1 hit.
SM00317. SET. 1 hit.
SM00466. SRA. 1 hit.
SUPFAMiSSF88697. SSF88697. 1 hit.
PROSITEiView protein in PROSITE
PS50868. POST_SET. 1 hit.
PS51575. SAM_MT43_SUVAR39_2. 1 hit.
PS50280. SET. 1 hit.
PS51015. YDG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C5P0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSTPPTLLM LFDDGDAGPS TGLVHREKSD AVNEEAHATS VPPHAPPQTL
60 70 80 90 100
WLLDNFNIED SYDRDAGPST GPVHRERSDA VNEEAHATSI PPHAPPQTLW
110 120 130 140 150
LLDNFNIEDS YDRDAGPSTS PIDREASHEV NEDAHATSAP PHVMVSPLQN
160 170 180 190 200
RRPFDQFNNQ PYDASAGPST GPGKRGRGRP KGSKNGSRKP KKPKAYDNNS
210 220 230 240 250
TDASAGPSSG LGKRRCGRPK GLKNRSRKPK KPKADDPNSK MVISCPDFDS
260 270 280 290 300
RITEAERESG NQEIVDSILM RFDAVRRRLC QLNYRKDKIL TASTNCMNLG
310 320 330 340 350
VRTNMTRRIG PIPGVQVGDI FYYWCEMCLV GLHRNTAGGI DSLLAKESGV
360 370 380 390 400
DGPAATSVVT SGKYDNETED LETLIYSGHG GKPCDQVLQR GNRALEASVR
410 420 430 440 450
RRNEVRVIRG ELYNNEKVYI YDGLYLVSDC WQVTGKSGFK EYRFKLLRKP
460 470 480 490 500
GQPPGYAIWK LVENLRNHEL IDPRQGFILG DLSFGEEGLR VPLVNEVDEE
510 520 530 540 550
DKTIPDDFDY IRSQCYSGMT NDVNVDSQSL VQSYIHQNCT CILKNCGQLP
560 570 580 590 600
YHDNILVCRK PLIYECGGSC PTRMVETGLK LHLEVFKTSN CGWGLRSWDP
610 620 630 640 650
IRAGTFICEF TGVSKTKEEV EEDDDYLFDT SRIYHSFRWN YEPELLCEDA
660 670 680 690 700
CEQVSEDANL PTQVLISAKE KGNVGRFMNH NCWPNVFWQP IEYDDNNGHI
710 720 730 740 750
YVRIGLFAMK HIPPMTELTY DYGISCVEKT GEDEVIYKGK KICLCGSVKC

RGSFG
Length:755
Mass (Da):84,528
Last modified:June 1, 2001 - v1
Checksum:i4B5BF379B8BF0C27
GO

Sequence cautioni

The sequence AAD26896 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF344451 Genomic DNA. Translation: AAK28973.1.
AC007266 Genomic DNA. Translation: AAD26896.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC07623.1.
PIRiC84640.
RefSeqiNP_180049.2. NM_128034.2.
UniGeneiAt.66241.

Genome annotation databases

EnsemblPlantsiAT2G24740.1; AT2G24740.1; AT2G24740.
GeneIDi817010.
GrameneiAT2G24740.1; AT2G24740.1; AT2G24740.
KEGGiath:AT2G24740.

Similar proteinsi

Entry informationi

Entry nameiSUVH8_ARATH
AccessioniPrimary (citable) accession number: Q9C5P0
Secondary accession number(s): Q9TP24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families