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Protein

Lupeol synthase 1

Gene

LUP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that converts oxidosqualene to lupeol and 3,20-dihydroxylupane. Minor production of beta-amyrin, germanicol, taraxasterol and psi-taraxasterol.2 Publications

Catalytic activityi

Lupan-3-beta,20-diol = (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O.
(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol.

GO - Molecular functioni

  • beta-amyrin synthase activity Source: TAIR
  • lupeol synthase activity Source: TAIR
  • lyase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Enzyme and pathway databases

BioCyciARA:GQT-1793-MONOMER.
BRENDAi4.2.1.128. 399.
5.4.99.41. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Lupeol synthase 1 (EC:4.2.1.128, EC:5.4.99.41)
Short name:
AtLUP1
Alternative name(s):
Lupan-3-beta,20-diol synthase
Gene namesi
Name:LUP1
Ordered Locus Names:At1g78970
ORF Names:YUP8H12R.28, YUP8H12R.42
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G78970.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Lupeol synthase 1PRO_0000366131Add
BLAST

Proteomic databases

PaxDbiQ9C5M3.
PRIDEiQ9C5M3.

Expressioni

Gene expression databases

GenevisibleiQ9C5M3. AT.

Interactioni

Protein-protein interaction databases

BioGridi29456. 2 interactions.
STRINGi3702.AT1G78970.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5M3.
SMRiQ9C5M3. Positions 99-277, 405-753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati149 – 19042PFTB 1Add
BLAST
Repeati589 – 62941PFTB 2Add
BLAST
Repeati638 – 67942PFTB 3Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 3 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ9C5M3.
KOiK15816.
OMAiEFMANTM.
OrthoDBiEOG0936031Q.
PhylomeDBiQ9C5M3.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C5M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKLKIGKGN GEDPHLFSSN NFVGRQTWKF DHKAGSPEER AAVEEARRGF
60 70 80 90 100
LDNRFRVKGC SDLLWRMQFL REKKFEQGIP QLKATNIEEI TYETTTNALR
110 120 130 140 150
RGVRYFTALQ ASDGHWPGEI TGPLFFLPPL IFCLYITGHL EEVFDAEHRK
160 170 180 190 200
EMLRHIYCHQ NEDGGWGLHI ESKSVMFCTV LNYICLRMLG ENPEQDACKR
210 220 230 240 250
ARQWILDRGG VIFIPSWGKF WLSILGVYDW SGTNPTPPEL LMLPSFLPIH
260 270 280 290 300
PGKILCYSRM VSIPMSYLYG KRFVGPITPL ILLLREELYL EPYEEINWKK
310 320 330 340 350
SRRLYAKEDM YYAHPLVQDL LSDTLQNFVE PLLTRWPLNK LVREKALQLT
360 370 380 390 400
MKHIHYEDEN SHYITIGCVE KVLCMLACWV ENPNGDYFKK HLARIPDYMW
410 420 430 440 450
VAEDGMKMQS FGCQLWDTGF AIQALLASNL PDETDDALKR GHNYIKASQV
460 470 480 490 500
RENPSGDFRS MYRHISKGAW TFSDRDHGWQ VSDCTAEALK CCLLLSMMSA
510 520 530 540 550
DIVGQKIDDE QLYDSVNLLL SLQSGNGGVN AWEPSRAYKW LELLNPTEFM
560 570 580 590 600
ANTMVEREFV ECTSSVIQAL DLFRKLYPDH RKKEINRSIE KAVQFIQDNQ
610 620 630 640 650
TPDGSWYGNW GVCFIYATWF ALGGLAAAGE TYNDCLAMRN GVHFLLTTQR
660 670 680 690 700
DDGGWGESYL SCSEQRYIPS EGERSNLVQT SWAMMALIHT GQAERDLIPL
710 720 730 740 750
HRAAKLIINS QLENGDFPQQ EIVGAFMNTC MLHYATYRNT FPLWALAEYR

KVVFIVN
Length:757
Mass (Da):87,352
Last modified:June 1, 2001 - v1
Checksum:i98C1C0C30D7975D5
GO

Sequence cautioni

The sequence AAC17055 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti466 – 4661S → W in AAB94341 (PubMed:11247608).Curated
Sequence conflicti503 – 5031V → G in AAD05032 (PubMed:9883589).Curated
Sequence conflicti698 – 6981I → T in AAD05032 (PubMed:9883589).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49919 mRNA. Translation: AAD05032.1.
U87266 mRNA. Translation: AAB94341.1.
AC002986 Genomic DNA. Translation: AAC17055.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36186.1.
CP002684 Genomic DNA. Translation: AEE36187.1.
AF360147 mRNA. Translation: AAK25857.1.
AY052358 mRNA. Translation: AAK96549.1.
AY142678 mRNA. Translation: AAN13216.1.
PIRiT01058.
RefSeqiNP_178018.1. NM_106546.2.
NP_849903.1. NM_179572.1.
UniGeneiAt.19007.

Genome annotation databases

EnsemblPlantsiAT1G78970.1; AT1G78970.1; AT1G78970.
AT1G78970.2; AT1G78970.2; AT1G78970.
GeneIDi844237.
GrameneiAT1G78970.1; AT1G78970.1; AT1G78970.
AT1G78970.2; AT1G78970.2; AT1G78970.
KEGGiath:AT1G78970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49919 mRNA. Translation: AAD05032.1.
U87266 mRNA. Translation: AAB94341.1.
AC002986 Genomic DNA. Translation: AAC17055.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE36186.1.
CP002684 Genomic DNA. Translation: AEE36187.1.
AF360147 mRNA. Translation: AAK25857.1.
AY052358 mRNA. Translation: AAK96549.1.
AY142678 mRNA. Translation: AAN13216.1.
PIRiT01058.
RefSeqiNP_178018.1. NM_106546.2.
NP_849903.1. NM_179572.1.
UniGeneiAt.19007.

3D structure databases

ProteinModelPortaliQ9C5M3.
SMRiQ9C5M3. Positions 99-277, 405-753.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29456. 2 interactions.
STRINGi3702.AT1G78970.1.

Proteomic databases

PaxDbiQ9C5M3.
PRIDEiQ9C5M3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G78970.1; AT1G78970.1; AT1G78970.
AT1G78970.2; AT1G78970.2; AT1G78970.
GeneIDi844237.
GrameneiAT1G78970.1; AT1G78970.1; AT1G78970.
AT1G78970.2; AT1G78970.2; AT1G78970.
KEGGiath:AT1G78970.

Organism-specific databases

TAIRiAT1G78970.

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ9C5M3.
KOiK15816.
OMAiEFMANTM.
OrthoDBiEOG0936031Q.
PhylomeDBiQ9C5M3.

Enzyme and pathway databases

BioCyciARA:GQT-1793-MONOMER.
BRENDAi4.2.1.128. 399.
5.4.99.41. 399.

Miscellaneous databases

PROiQ9C5M3.

Gene expression databases

GenevisibleiQ9C5M3. AT.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUP1_ARATH
AccessioniPrimary (citable) accession number: Q9C5M3
Secondary accession number(s): O49985, O64551, P92977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.