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Q9C5M0

- DTC_ARATH

UniProt

Q9C5M0 - DTC_ARATH

Protein

Mitochondrial dicarboxylate/tricarboxylate transporter DTC

Gene

DTC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the transport of dicarboxylates, such as oxoglutarate, oxaloacetate, malate, and succinate, and of tricarboxylates, such as citrate, isocitrate, cis-aconitate, and trans-aconitate by a counter-exchange mechanism across the inner mitochondrial membrane. Substrate preference in reconstituted proteoliposomes is oxaloacetate > malonate > malate > maleate > succinate > oxoglutarate > citrate > trans-aconitate > cis-aconitate > sulfate > isocitrate. May be important for plant metabolic functions requiring organic acid flux to or from the mitochondria, such as nitrogen assimilation, export of reducing equivalents from the mitochondria, and fatty acid elongation.1 Publication

    Kineticsi

    1. KM=0.14 mM for oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
    2. KM=0.36 mM for malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
    3. KM=0.15 mM for citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

    Vmax=2.9 mmol/min/g enzyme toward oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

    Vmax=4.2 mmol/min/g enzyme toward malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

    Vmax=1.7 mmol/min/g enzyme toward citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

    pH dependencei

    Optimum pH is 5.5-6.0.1 Publication

    GO - Molecular functioni

    1. dicarboxylic acid transmembrane transporter activity Source: TAIR
    2. tricarboxylic acid transmembrane transporter activity Source: TAIR

    GO - Biological processi

    1. dicarboxylic acid transport Source: TAIR
    2. tricarboxylic acid transmembrane transport Source: TAIR

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial dicarboxylate/tricarboxylate transporter DTC
    Alternative name(s):
    Dicarboxylate/tricarboxylate carrier
    Gene namesi
    Name:DTC
    Ordered Locus Names:At5g19760
    ORF Names:T29J13.180
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G19760.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. chloroplast Source: TAIR
    3. chloroplast envelope Source: TAIR
    4. integral component of membrane Source: UniProtKB-KW
    5. mitochondrial inner membrane Source: UniProtKB-SubCell
    6. mitochondrion Source: TAIR
    7. plasmodesma Source: TAIR
    8. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Mitochondrial dicarboxylate/tricarboxylate transporter DTCPRO_0000420761Add
    BLAST

    Proteomic databases

    PRIDEiQ9C5M0.

    Expressioni

    Tissue specificityi

    Highly expressed in flower buds and at lower levels in roots, leaves and stems.1 Publication

    Gene expression databases

    GenevestigatoriQ9C5M0.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9C5M0. 2 interactions.
    STRINGi3702.AT5G19760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5M0.
    SMRiQ9C5M0. Positions 14-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei18 – 3821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei68 – 8821Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei109 – 12921Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei169 – 18921Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei208 – 22821Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei268 – 28821Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati12 – 9382Solcar 1Add
    BLAST
    Repeati103 – 19492Solcar 2Add
    BLAST
    Repeati202 – 29291Solcar 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 Solcar repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000165139.
    InParanoidiQ9C5M0.
    KOiK15104.
    OMAiFRISKHE.
    PhylomeDBiQ9C5M0.

    Family and domain databases

    Gene3Di1.50.40.10. 1 hit.
    InterProiIPR002067. Mit_carrier.
    IPR018108. Mitochondrial_sb/sol_carrier.
    IPR023395. Mt_carrier_dom.
    [Graphical view]
    PfamiPF00153. Mito_carr. 3 hits.
    [Graphical view]
    PRINTSiPR00926. MITOCARRIER.
    SUPFAMiSSF103506. SSF103506. 1 hit.
    PROSITEiPS50920. SOLCAR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9C5M0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEEKKAPIS VWTTVKPFVN GGASGMLATC VIQPIDMIKV RIQLGQGSAA    50
    SITTNMLKNE GVGAFYKGLS AGLLRQATYT TARLGSFKLL TAKAIESNDG 100
    KPLPLYQKAL CGLTAGAIGA CVGSPADLAL IRMQADNTLP LAQRRNYTNA 150
    FHALTRISAD EGVLALWKGC GPTVVRAMAL NMGMLASYDQ SAEYMRDNLG 200
    FGEMSTVVGA SAVSGFCAAA CSLPFDFVKT QIQKMQPDAQ GKYPYTGSLD 250
    CAMKTLKEGG PLKFYSGFPV YCVRIAPHVM MTWIFLNQIT KFQKKIGM 298
    Length:298
    Mass (Da):31,912
    Last modified:June 1, 2001 - v1
    Checksum:i2609F4FE1D991628
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ311780 mRNA. Translation: CAC84549.1.
    AF296838 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92746.1.
    AF360153 mRNA. Translation: AAK25863.1.
    AY056307 mRNA. Translation: AAL07156.1.
    AY085901 mRNA. Translation: AAM63113.1.
    AK226470 mRNA. Translation: BAE98612.1.
    RefSeqiNP_197477.1. NM_121981.4.
    UniGeneiAt.24504.
    At.24857.
    At.49038.

    Genome annotation databases

    EnsemblPlantsiAT5G19760.1; AT5G19760.1; AT5G19760.
    GeneIDi832096.
    KEGGiath:AT5G19760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ311780 mRNA. Translation: CAC84549.1 .
    AF296838 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92746.1 .
    AF360153 mRNA. Translation: AAK25863.1 .
    AY056307 mRNA. Translation: AAL07156.1 .
    AY085901 mRNA. Translation: AAM63113.1 .
    AK226470 mRNA. Translation: BAE98612.1 .
    RefSeqi NP_197477.1. NM_121981.4.
    UniGenei At.24504.
    At.24857.
    At.49038.

    3D structure databases

    ProteinModelPortali Q9C5M0.
    SMRi Q9C5M0. Positions 14-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9C5M0. 2 interactions.
    STRINGi 3702.AT5G19760.1-P.

    Proteomic databases

    PRIDEi Q9C5M0.

    Protocols and materials databases

    DNASUi 832096.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G19760.1 ; AT5G19760.1 ; AT5G19760 .
    GeneIDi 832096.
    KEGGi ath:AT5G19760.

    Organism-specific databases

    GeneFarmi 3826.
    TAIRi AT5G19760.

    Phylogenomic databases

    HOGENOMi HOG000165139.
    InParanoidi Q9C5M0.
    KOi K15104.
    OMAi FRISKHE.
    PhylomeDBi Q9C5M0.

    Miscellaneous databases

    PROi Q9C5M0.

    Gene expression databases

    Genevestigatori Q9C5M0.

    Family and domain databases

    Gene3Di 1.50.40.10. 1 hit.
    InterProi IPR002067. Mit_carrier.
    IPR018108. Mitochondrial_sb/sol_carrier.
    IPR023395. Mt_carrier_dom.
    [Graphical view ]
    Pfami PF00153. Mito_carr. 3 hits.
    [Graphical view ]
    PRINTSi PR00926. MITOCARRIER.
    SUPFAMi SSF103506. SSF103506. 1 hit.
    PROSITEi PS50920. SOLCAR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution."
      Picault N., Palmieri L., Pisano I., Hodges M., Palmieri F.
      J. Biol. Chem. 277:24204-24211(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Genomic and proteomic analysis of mitochondrial carrier proteins in Arabidopsis."
      Millar A.H., Heazlewood J.L.
      Plant Physiol. 131:443-453(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiDTC_ARATH
    AccessioniPrimary (citable) accession number: Q9C5M0
    Secondary accession number(s): Q8SFL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3