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Q9C5M0 (DTC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial dicarboxylate/tricarboxylate transporter DTC
Alternative name(s):
Dicarboxylate/tricarboxylate carrier
Gene names
Name:DTC
Ordered Locus Names:At5g19760
ORF Names:T29J13.180
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transport of dicarboxylates, such as oxoglutarate, oxaloacetate, malate, and succinate, and of tricarboxylates, such as citrate, isocitrate, cis-aconitate, and trans-aconitate by a counter-exchange mechanism across the inner mitochondrial membrane. Substrate preference in reconstituted proteoliposomes is oxaloacetate > malonate > malate > maleate > succinate > oxoglutarate > citrate > trans-aconitate > cis-aconitate > sulfate > isocitrate. May be important for plant metabolic functions requiring organic acid flux to or from the mitochondria, such as nitrogen assimilation, export of reducing equivalents from the mitochondria, and fatty acid elongation. Ref.1

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Probable Ref.6.

Tissue specificity

Highly expressed in flower buds and at lower levels in roots, leaves and stems. Ref.1

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Biophysicochemical properties

Kinetic parameters:

KM=0.14 mM for oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0) Ref.1

KM=0.36 mM for malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)

KM=0.15 mM for citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)

Vmax=2.9 mmol/min/g enzyme toward oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)

Vmax=4.2 mmol/min/g enzyme toward malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)

Vmax=1.7 mmol/min/g enzyme toward citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)

pH dependence:

Optimum pH is 5.5-6.0.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Mitochondrial dicarboxylate/tricarboxylate transporter DTC
PRO_0000420761

Regions

Transmembrane18 – 3821Helical; Name=1; Potential
Transmembrane68 – 8821Helical; Name=2; Potential
Transmembrane109 – 12921Helical; Name=3; Potential
Transmembrane169 – 18921Helical; Name=4; Potential
Transmembrane208 – 22821Helical; Name=5; Potential
Transmembrane268 – 28821Helical; Name=6; Potential
Repeat12 – 9382Solcar 1
Repeat103 – 19492Solcar 2
Repeat202 – 29291Solcar 3

Sequences

Sequence LengthMass (Da)Tools
Q9C5M0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 2609F4FE1D991628

FASTA29831,912
        10         20         30         40         50         60 
MAEEKKAPIS VWTTVKPFVN GGASGMLATC VIQPIDMIKV RIQLGQGSAA SITTNMLKNE 

        70         80         90        100        110        120 
GVGAFYKGLS AGLLRQATYT TARLGSFKLL TAKAIESNDG KPLPLYQKAL CGLTAGAIGA 

       130        140        150        160        170        180 
CVGSPADLAL IRMQADNTLP LAQRRNYTNA FHALTRISAD EGVLALWKGC GPTVVRAMAL 

       190        200        210        220        230        240 
NMGMLASYDQ SAEYMRDNLG FGEMSTVVGA SAVSGFCAAA CSLPFDFVKT QIQKMQPDAQ 

       250        260        270        280        290 
GKYPYTGSLD CAMKTLKEGG PLKFYSGFPV YCVRIAPHVM MTWIFLNQIT KFQKKIGM 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution."
Picault N., Palmieri L., Pisano I., Hodges M., Palmieri F.
J. Biol. Chem. 277:24204-24211(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Genomic and proteomic analysis of mitochondrial carrier proteins in Arabidopsis."
Millar A.H., Heazlewood J.L.
Plant Physiol. 131:443-453(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ311780 mRNA. Translation: CAC84549.1.
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92746.1.
AF360153 mRNA. Translation: AAK25863.1.
AY056307 mRNA. Translation: AAL07156.1.
AY085901 mRNA. Translation: AAM63113.1.
AK226470 mRNA. Translation: BAE98612.1.
RefSeqNP_197477.1. NM_121981.4.
UniGeneAt.24504.
At.24857.
At.49038.

3D structure databases

ProteinModelPortalQ9C5M0.
SMRQ9C5M0. Positions 14-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9C5M0. 1 interaction.
STRING3702.AT5G19760.1-P.

Proteomic databases

PRIDEQ9C5M0.

Protocols and materials databases

DNASU832096.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G19760.1; AT5G19760.1; AT5G19760.
GeneID832096.
KEGGath:AT5G19760.

Organism-specific databases

GeneFarm3826.
TAIRAT5G19760.

Phylogenomic databases

HOGENOMHOG000165139.
InParanoidQ9C5M0.
KOK15104.
OMAFRISKHE.
PhylomeDBQ9C5M0.

Gene expression databases

GenevestigatorQ9C5M0.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSPR00926. MITOCARRIER.
SUPFAMSSF103506. SSF103506. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

PROQ9C5M0.

Entry information

Entry nameDTC_ARATH
AccessionPrimary (citable) accession number: Q9C5M0
Secondary accession number(s): Q8SFL2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names