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Protein

Mitochondrial dicarboxylate/tricarboxylate transporter DTC

Gene

DTC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transport of dicarboxylates, such as oxoglutarate, oxaloacetate, malate, and succinate, and of tricarboxylates, such as citrate, isocitrate, cis-aconitate, and trans-aconitate by a counter-exchange mechanism across the inner mitochondrial membrane. Substrate preference in reconstituted proteoliposomes is oxaloacetate > malonate > malate > maleate > succinate > oxoglutarate > citrate > trans-aconitate > cis-aconitate > sulfate > isocitrate. May be important for plant metabolic functions requiring organic acid flux to or from the mitochondria, such as nitrogen assimilation, export of reducing equivalents from the mitochondria, and fatty acid elongation.1 Publication

Kineticsi

  1. KM=0.14 mM for oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  2. KM=0.36 mM for malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  3. KM=0.15 mM for citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  1. Vmax=2.9 mmol/min/g enzyme toward oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  2. Vmax=4.2 mmol/min/g enzyme toward malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  3. Vmax=1.7 mmol/min/g enzyme toward citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

GO - Molecular functioni

  • dicarboxylic acid transmembrane transporter activity Source: TAIR
  • tricarboxylic acid transmembrane transporter activity Source: TAIR

GO - Biological processi

  • dicarboxylic acid transport Source: TAIR
  • tricarboxylic acid transmembrane transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dicarboxylate/tricarboxylate transporter DTC
Alternative name(s):
Dicarboxylate/tricarboxylate carrier
Gene namesi
Name:DTC
Ordered Locus Names:At5g19760
ORF Names:T29J13.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19760.

Subcellular locationi

  • Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei18 – 38Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei68 – 88Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei109 – 129Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei169 – 189Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei208 – 228Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei268 – 288Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004207611 – 298Mitochondrial dicarboxylate/tricarboxylate transporter DTCAdd BLAST298

Proteomic databases

PaxDbiQ9C5M0.
PRIDEiQ9C5M0.

Expressioni

Tissue specificityi

Highly expressed in flower buds and at lower levels in roots, leaves and stems.1 Publication

Gene expression databases

GenevisibleiQ9C5M0. AT.

Interactioni

Protein-protein interaction databases

BioGridi17372. 1 interactor.
IntActiQ9C5M0. 2 interactors.
STRINGi3702.AT5G19760.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5M0.
SMRiQ9C5M0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati12 – 93Solcar 1Add BLAST82
Repeati103 – 194Solcar 2Add BLAST92
Repeati202 – 292Solcar 3Add BLAST91

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0759. Eukaryota.
ENOG410XQHU. LUCA.
HOGENOMiHOG000165139.
InParanoidiQ9C5M0.
KOiK15104.
OMAiSDNIFCH.
OrthoDBiEOG09360HR9.
PhylomeDBiQ9C5M0.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C5M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEKKAPIS VWTTVKPFVN GGASGMLATC VIQPIDMIKV RIQLGQGSAA
60 70 80 90 100
SITTNMLKNE GVGAFYKGLS AGLLRQATYT TARLGSFKLL TAKAIESNDG
110 120 130 140 150
KPLPLYQKAL CGLTAGAIGA CVGSPADLAL IRMQADNTLP LAQRRNYTNA
160 170 180 190 200
FHALTRISAD EGVLALWKGC GPTVVRAMAL NMGMLASYDQ SAEYMRDNLG
210 220 230 240 250
FGEMSTVVGA SAVSGFCAAA CSLPFDFVKT QIQKMQPDAQ GKYPYTGSLD
260 270 280 290
CAMKTLKEGG PLKFYSGFPV YCVRIAPHVM MTWIFLNQIT KFQKKIGM
Length:298
Mass (Da):31,912
Last modified:June 1, 2001 - v1
Checksum:i2609F4FE1D991628
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311780 mRNA. Translation: CAC84549.1.
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92746.1.
AF360153 mRNA. Translation: AAK25863.1.
AY056307 mRNA. Translation: AAL07156.1.
AY085901 mRNA. Translation: AAM63113.1.
AK226470 mRNA. Translation: BAE98612.1.
RefSeqiNP_197477.1. NM_121981.5.
UniGeneiAt.24504.
At.24857.
At.49038.

Genome annotation databases

EnsemblPlantsiAT5G19760.1; AT5G19760.1; AT5G19760.
GeneIDi832096.
GrameneiAT5G19760.1; AT5G19760.1; AT5G19760.
KEGGiath:AT5G19760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311780 mRNA. Translation: CAC84549.1.
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92746.1.
AF360153 mRNA. Translation: AAK25863.1.
AY056307 mRNA. Translation: AAL07156.1.
AY085901 mRNA. Translation: AAM63113.1.
AK226470 mRNA. Translation: BAE98612.1.
RefSeqiNP_197477.1. NM_121981.5.
UniGeneiAt.24504.
At.24857.
At.49038.

3D structure databases

ProteinModelPortaliQ9C5M0.
SMRiQ9C5M0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17372. 1 interactor.
IntActiQ9C5M0. 2 interactors.
STRINGi3702.AT5G19760.1.

Proteomic databases

PaxDbiQ9C5M0.
PRIDEiQ9C5M0.

Protocols and materials databases

DNASUi832096.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19760.1; AT5G19760.1; AT5G19760.
GeneIDi832096.
GrameneiAT5G19760.1; AT5G19760.1; AT5G19760.
KEGGiath:AT5G19760.

Organism-specific databases

TAIRiAT5G19760.

Phylogenomic databases

eggNOGiKOG0759. Eukaryota.
ENOG410XQHU. LUCA.
HOGENOMiHOG000165139.
InParanoidiQ9C5M0.
KOiK15104.
OMAiSDNIFCH.
OrthoDBiEOG09360HR9.
PhylomeDBiQ9C5M0.

Miscellaneous databases

PROiQ9C5M0.

Gene expression databases

GenevisibleiQ9C5M0. AT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDTC_ARATH
AccessioniPrimary (citable) accession number: Q9C5M0
Secondary accession number(s): Q8SFL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.