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Q9C5M0

- DTC_ARATH

UniProt

Q9C5M0 - DTC_ARATH

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Protein

Mitochondrial dicarboxylate/tricarboxylate transporter DTC

Gene

DTC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transport of dicarboxylates, such as oxoglutarate, oxaloacetate, malate, and succinate, and of tricarboxylates, such as citrate, isocitrate, cis-aconitate, and trans-aconitate by a counter-exchange mechanism across the inner mitochondrial membrane. Substrate preference in reconstituted proteoliposomes is oxaloacetate > malonate > malate > maleate > succinate > oxoglutarate > citrate > trans-aconitate > cis-aconitate > sulfate > isocitrate. May be important for plant metabolic functions requiring organic acid flux to or from the mitochondria, such as nitrogen assimilation, export of reducing equivalents from the mitochondria, and fatty acid elongation.1 Publication

Kineticsi

  1. KM=0.14 mM for oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  2. KM=0.36 mM for malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication
  3. KM=0.15 mM for citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

Vmax=2.9 mmol/min/g enzyme toward oxoglutarate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

Vmax=4.2 mmol/min/g enzyme toward malate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

Vmax=1.7 mmol/min/g enzyme toward citrate (for the recombinant protein in reconstituted proteoliposomes at pH 6.0)1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

GO - Molecular functioni

  1. dicarboxylic acid transmembrane transporter activity Source: TAIR
  2. tricarboxylic acid transmembrane transporter activity Source: TAIR

GO - Biological processi

  1. dicarboxylic acid transport Source: TAIR
  2. tricarboxylic acid transmembrane transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dicarboxylate/tricarboxylate transporter DTC
Alternative name(s):
Dicarboxylate/tricarboxylate carrier
Gene namesi
Name:DTC
Ordered Locus Names:At5g19760
ORF Names:T29J13.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G19760.

Subcellular locationi

Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast envelope Source: TAIR
  4. integral component of membrane Source: UniProtKB-KW
  5. mitochondrial inner membrane Source: UniProtKB-KW
  6. mitochondrion Source: TAIR
  7. plasmodesma Source: TAIR
  8. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Mitochondrial dicarboxylate/tricarboxylate transporter DTCPRO_0000420761Add
BLAST

Proteomic databases

PRIDEiQ9C5M0.

Expressioni

Tissue specificityi

Highly expressed in flower buds and at lower levels in roots, leaves and stems.1 Publication

Gene expression databases

GenevestigatoriQ9C5M0.

Interactioni

Protein-protein interaction databases

IntActiQ9C5M0. 2 interactions.
STRINGi3702.AT5G19760.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5M0.
SMRiQ9C5M0. Positions 14-291.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei68 – 8821Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei109 – 12921Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei169 – 18921Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei208 – 22821Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei268 – 28821Helical; Name=6Sequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati12 – 9382Solcar 1Add
BLAST
Repeati103 – 19492Solcar 2Add
BLAST
Repeati202 – 29291Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000165139.
InParanoidiQ9C5M0.
KOiK15104.
OMAiFRISKHE.
PhylomeDBiQ9C5M0.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C5M0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEEKKAPIS VWTTVKPFVN GGASGMLATC VIQPIDMIKV RIQLGQGSAA
60 70 80 90 100
SITTNMLKNE GVGAFYKGLS AGLLRQATYT TARLGSFKLL TAKAIESNDG
110 120 130 140 150
KPLPLYQKAL CGLTAGAIGA CVGSPADLAL IRMQADNTLP LAQRRNYTNA
160 170 180 190 200
FHALTRISAD EGVLALWKGC GPTVVRAMAL NMGMLASYDQ SAEYMRDNLG
210 220 230 240 250
FGEMSTVVGA SAVSGFCAAA CSLPFDFVKT QIQKMQPDAQ GKYPYTGSLD
260 270 280 290
CAMKTLKEGG PLKFYSGFPV YCVRIAPHVM MTWIFLNQIT KFQKKIGM
Length:298
Mass (Da):31,912
Last modified:June 1, 2001 - v1
Checksum:i2609F4FE1D991628
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ311780 mRNA. Translation: CAC84549.1.
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92746.1.
AF360153 mRNA. Translation: AAK25863.1.
AY056307 mRNA. Translation: AAL07156.1.
AY085901 mRNA. Translation: AAM63113.1.
AK226470 mRNA. Translation: BAE98612.1.
RefSeqiNP_197477.1. NM_121981.4.
UniGeneiAt.24504.
At.24857.
At.49038.

Genome annotation databases

EnsemblPlantsiAT5G19760.1; AT5G19760.1; AT5G19760.
GeneIDi832096.
KEGGiath:AT5G19760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ311780 mRNA. Translation: CAC84549.1 .
AF296838 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92746.1 .
AF360153 mRNA. Translation: AAK25863.1 .
AY056307 mRNA. Translation: AAL07156.1 .
AY085901 mRNA. Translation: AAM63113.1 .
AK226470 mRNA. Translation: BAE98612.1 .
RefSeqi NP_197477.1. NM_121981.4.
UniGenei At.24504.
At.24857.
At.49038.

3D structure databases

ProteinModelPortali Q9C5M0.
SMRi Q9C5M0. Positions 14-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9C5M0. 2 interactions.
STRINGi 3702.AT5G19760.1-P.

Proteomic databases

PRIDEi Q9C5M0.

Protocols and materials databases

DNASUi 832096.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G19760.1 ; AT5G19760.1 ; AT5G19760 .
GeneIDi 832096.
KEGGi ath:AT5G19760.

Organism-specific databases

GeneFarmi 3826.
TAIRi AT5G19760.

Phylogenomic databases

HOGENOMi HOG000165139.
InParanoidi Q9C5M0.
KOi K15104.
OMAi FRISKHE.
PhylomeDBi Q9C5M0.

Miscellaneous databases

PROi Q9C5M0.

Gene expression databases

Genevestigatori Q9C5M0.

Family and domain databases

Gene3Di 1.50.40.10. 1 hit.
InterProi IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view ]
Pfami PF00153. Mito_carr. 3 hits.
[Graphical view ]
PRINTSi PR00926. MITOCARRIER.
SUPFAMi SSF103506. SSF103506. 1 hit.
PROSITEi PS50920. SOLCAR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel transporter for dicarboxylates and tricarboxylates in plant mitochondria. Bacterial expression, reconstitution, functional characterization, and tissue distribution."
    Picault N., Palmieri L., Pisano I., Hodges M., Palmieri F.
    J. Biol. Chem. 277:24204-24211(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Genomic and proteomic analysis of mitochondrial carrier proteins in Arabidopsis."
    Millar A.H., Heazlewood J.L.
    Plant Physiol. 131:443-453(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiDTC_ARATH
AccessioniPrimary (citable) accession number: Q9C5M0
Secondary accession number(s): Q8SFL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3