Q9C5J7 (K6PF7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 7
EC=2.7.1.11

Alternative name(s):
Phosphofructokinase 7
Phosphohexokinase 7

Gene names

Name:	PFK7
Ordered Locus Names:	At5g56630
ORF Names:	MIK19.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. Ref.4
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subcellular location	Cytoplasm Ref.4.
Tissue specificity	Expressed in roots, leaves, stems and flowers. Ref.4
Sequence similarities	Belongs to the phosphofructokinase family.
Sequence caution	The sequence BAB09881.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from direct assay Ref.4. Source: TAIR
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro cytosol Inferred from direct assay Ref.4PubMed 21166475. Source: TAIR
Molecular_function	6-phosphofructokinase activity Inferred from direct assay Ref.4. Source: TAIR ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	6-phosphofructokinase 7		PRO_0000330774
Regions
	Nucleotide binding	111 – 115	5	ATP By similarity
	Nucleotide binding	247 – 251	5	ATP By similarity
	Nucleotide binding	265 – 282	18	ATP By similarity
Sites
	Active site	220	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C5J7 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 6F0C6CFF43EAA7B5	FASTA	485	53,482
	10 20 30 40 50 60 MSSPRSNKPK IVNGPGGYIL QDVPHLIDYL PDLPTYPNPL QDNPAYSVVK QYFVHADDSV 70 80 90 100 110 120 PEKVVVHKDG PRGVHFRRAG PRQKVYFESD EVHACIVTCG GLCPGLNTVI REVVSSLSYM 130 140 150 160 170 180 YGVKRILGID GGYRGFYAKN TIPLNSKVVN DIHKRGGTII GTSRGGHDTN KIVDSIQDRG 190 200 210 220 230 240 INQVYIIGGD GTQRGASVIF EEIRRRRLKV AVVGIPKTID NDIPVIDKSF GFDTAVEEAQ 250 260 270 280 290 300 RAINAAHVEA ESNENGIGFV KLMGRYSGYI AMYATLASRD VDCCLIPESP FYLEGEGGLF 310 320 330 340 350 360 EFIERRLKDH GHMVIVLAEG AGQDLMCKSM ESTPMDASGN KLLKDVGLWL SQSIKDHFKK 370 380 390 400 410 420 NKMVMNLKYI DPTYMIRAVP SNASDNVYCT LLAQSAVHGA MAGYTGYTSG LVNGRQTYIP 430 440 450 460 470 480 FYRITETQNN VVITDRMWAR LLSSTNQPSF LGPKDTSEEK KELPETPLLD DGAVDIPPVT KEVTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana." Mustroph A., Sonnewald U., Biemelt S. FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems. CP002688 Genomic DNA. Translation: AED96790.1. AF360207 mRNA. Translation: AAK25917.1. AY040055 mRNA. Translation: AAK64113.1.
IPI	IPI00533425.
RefSeq	NP_568842.1. NM_125046.2.
UniGene	At.9267.
3D structure databases
HSSP	HSSP built from PDB template 1MTO based on UniProtKB P00512.
ProteinModelPortal	Q9C5J7.
SMR	Q9C5J7. Positions 27-441.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C5J7. 6 interactions.
STRING	3702.AT5G56630.1-P.
Proteomic databases
PaxDb	Q9C5J7.
PRIDE	Q9C5J7.
Protocols and materials databases
DNASU	835764.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G56630.1; AT5G56630.1; AT5G56630.
GeneID	835764.
KEGG	ath:AT5G56630.
Organism-specific databases
TAIR	At5g56630.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000016186.
InParanoid	Q9C5J7.
KO	K00850.
OMA	RITETQN.
PhylomeDB	Q9C5J7.
ProtClustDB	PLN02564.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Gene expression databases
Genevestigator	Q9C5J7.
Family and domain databases
InterPro	IPR022953. Phosphofructokinase. IPR000023. Phosphofructokinase_dom. IPR012004. PyroP-dep_PFruKinase_TP0108. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

K6PF7_ARATH

Accession

Primary (citable) accession number: Q9C5J7
Secondary accession number(s): Q9FJU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program