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Q9C5J7

- PFKA7_ARATH

UniProt

Q9C5J7 - PFKA7_ARATH

Protein

ATP-dependent 6-phosphofructokinase 7

Gene

PFK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011ATP; via amide nitrogenUniRule annotation
    Metal bindingi190 – 1901Magnesium; catalyticUniRule annotation
    Sitei191 – 1911Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei220 – 2201Proton acceptorUniRule annotation
    Binding sitei319 – 3191SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi164 – 1652ATPUniRule annotation
    Nucleotide bindingi189 – 1924ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: TAIR
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G56630-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 7UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 7UniRule annotation
    Short name:
    Phosphofructokinase 7UniRule annotation
    Alternative name(s):
    Phosphohexokinase 7UniRule annotation
    Gene namesi
    Name:PFK7UniRule annotation
    Ordered Locus Names:At5g56630
    ORF Names:MIK19.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G56630.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro
    2. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485ATP-dependent 6-phosphofructokinase 7PRO_0000330774Add
    BLAST

    Proteomic databases

    PaxDbiQ9C5J7.
    PRIDEiQ9C5J7.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ9C5J7.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    BioGridi21008. 5 interactions.
    IntActiQ9C5J7. 6 interactions.
    STRINGi3702.AT5G56630.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5J7.
    SMRiQ9C5J7. Positions 27-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2203Substrate bindingUniRule annotation
    Regioni263 – 2653Substrate bindingUniRule annotation
    Regioni374 – 3774Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000016186.
    InParanoidiQ9C5J7.
    KOiK00850.
    OMAiSNENGIG.
    PhylomeDBiQ9C5J7.

    Family and domain databases

    HAMAPiMF_01981. Phosphofructokinase_II_X.
    InterProiIPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9C5J7-1 [UniParc]FASTAAdd to Basket

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    MSSPRSNKPK IVNGPGGYIL QDVPHLIDYL PDLPTYPNPL QDNPAYSVVK    50
    QYFVHADDSV PEKVVVHKDG PRGVHFRRAG PRQKVYFESD EVHACIVTCG 100
    GLCPGLNTVI REVVSSLSYM YGVKRILGID GGYRGFYAKN TIPLNSKVVN 150
    DIHKRGGTII GTSRGGHDTN KIVDSIQDRG INQVYIIGGD GTQRGASVIF 200
    EEIRRRRLKV AVVGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA 250
    ESNENGIGFV KLMGRYSGYI AMYATLASRD VDCCLIPESP FYLEGEGGLF 300
    EFIERRLKDH GHMVIVLAEG AGQDLMCKSM ESTPMDASGN KLLKDVGLWL 350
    SQSIKDHFKK NKMVMNLKYI DPTYMIRAVP SNASDNVYCT LLAQSAVHGA 400
    MAGYTGYTSG LVNGRQTYIP FYRITETQNN VVITDRMWAR LLSSTNQPSF 450
    LGPKDTSEEK KELPETPLLD DGAVDIPPVT KEVTK 485
    Length:485
    Mass (Da):53,482
    Last modified:June 1, 2001 - v1
    Checksum:i6F0C6CFF43EAA7B5
    GO

    Sequence cautioni

    The sequence BAB09881.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED96790.1.
    AF360207 mRNA. Translation: AAK25917.1.
    AY040055 mRNA. Translation: AAK64113.1.
    RefSeqiNP_568842.1. NM_125046.2.
    UniGeneiAt.9267.

    Genome annotation databases

    EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630.
    GeneIDi835764.
    KEGGiath:AT5G56630.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013392 Genomic DNA. Translation: BAB09881.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED96790.1 .
    AF360207 mRNA. Translation: AAK25917.1 .
    AY040055 mRNA. Translation: AAK64113.1 .
    RefSeqi NP_568842.1. NM_125046.2.
    UniGenei At.9267.

    3D structure databases

    ProteinModelPortali Q9C5J7.
    SMRi Q9C5J7. Positions 27-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21008. 5 interactions.
    IntActi Q9C5J7. 6 interactions.
    STRINGi 3702.AT5G56630.1-P.

    Proteomic databases

    PaxDbi Q9C5J7.
    PRIDEi Q9C5J7.

    Protocols and materials databases

    DNASUi 835764.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G56630.1 ; AT5G56630.1 ; AT5G56630 .
    GeneIDi 835764.
    KEGGi ath:AT5G56630.

    Organism-specific databases

    TAIRi AT5G56630.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000016186.
    InParanoidi Q9C5J7.
    KOi K00850.
    OMAi SNENGIG.
    PhylomeDBi Q9C5J7.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci ARA:AT5G56630-MONOMER.

    Gene expression databases

    Genevestigatori Q9C5J7.

    Family and domain databases

    HAMAPi MF_01981. Phosphofructokinase_II_X.
    InterProi IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    IPR012004. PyroP-dep_PFruKinase_TP0108.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
      Mustroph A., Sonnewald U., Biemelt S.
      FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPFKA7_ARATH
    AccessioniPrimary (citable) accession number: Q9C5J7
    Secondary accession number(s): Q9FJU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3