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Protein

ATP-dependent 6-phosphofructokinase 7

Gene

PFK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATP; via amide nitrogenUniRule annotation
Metal bindingi190 – 1901Magnesium; catalyticUniRule annotation
Sitei191 – 1911Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei220 – 2201Proton acceptorUniRule annotation
Binding sitei319 – 3191SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1652ATPUniRule annotation
Nucleotide bindingi189 – 1924ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G56630-MONOMER.
ReactomeiREACT_248260. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 7UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 7UniRule annotation
Short name:
Phosphofructokinase 7UniRule annotation
Alternative name(s):
Phosphohexokinase 7UniRule annotation
Gene namesi
Name:PFK7UniRule annotation
Ordered Locus Names:At5g56630
ORF Names:MIK19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G56630.

Subcellular locationi

Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485ATP-dependent 6-phosphofructokinase 7PRO_0000330774Add
BLAST

Proteomic databases

PaxDbiQ9C5J7.
PRIDEiQ9C5J7.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

GenevestigatoriQ9C5J7.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi21008. 5 interactions.
IntActiQ9C5J7. 6 interactions.
STRINGi3702.AT5G56630.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5J7.
SMRiQ9C5J7. Positions 27-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2203Substrate bindingUniRule annotation
Regioni263 – 2653Substrate bindingUniRule annotation
Regioni374 – 3774Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9C5J7.
KOiK00850.
OMAiIICISEG.
PhylomeDBiQ9C5J7.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C5J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPRSNKPK IVNGPGGYIL QDVPHLIDYL PDLPTYPNPL QDNPAYSVVK
60 70 80 90 100
QYFVHADDSV PEKVVVHKDG PRGVHFRRAG PRQKVYFESD EVHACIVTCG
110 120 130 140 150
GLCPGLNTVI REVVSSLSYM YGVKRILGID GGYRGFYAKN TIPLNSKVVN
160 170 180 190 200
DIHKRGGTII GTSRGGHDTN KIVDSIQDRG INQVYIIGGD GTQRGASVIF
210 220 230 240 250
EEIRRRRLKV AVVGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA
260 270 280 290 300
ESNENGIGFV KLMGRYSGYI AMYATLASRD VDCCLIPESP FYLEGEGGLF
310 320 330 340 350
EFIERRLKDH GHMVIVLAEG AGQDLMCKSM ESTPMDASGN KLLKDVGLWL
360 370 380 390 400
SQSIKDHFKK NKMVMNLKYI DPTYMIRAVP SNASDNVYCT LLAQSAVHGA
410 420 430 440 450
MAGYTGYTSG LVNGRQTYIP FYRITETQNN VVITDRMWAR LLSSTNQPSF
460 470 480
LGPKDTSEEK KELPETPLLD DGAVDIPPVT KEVTK
Length:485
Mass (Da):53,482
Last modified:June 1, 2001 - v1
Checksum:i6F0C6CFF43EAA7B5
GO

Sequence cautioni

The sequence BAB09881.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96790.1.
AF360207 mRNA. Translation: AAK25917.1.
AY040055 mRNA. Translation: AAK64113.1.
RefSeqiNP_568842.1. NM_125046.2.
UniGeneiAt.9267.

Genome annotation databases

EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630.
GeneIDi835764.
KEGGiath:AT5G56630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96790.1.
AF360207 mRNA. Translation: AAK25917.1.
AY040055 mRNA. Translation: AAK64113.1.
RefSeqiNP_568842.1. NM_125046.2.
UniGeneiAt.9267.

3D structure databases

ProteinModelPortaliQ9C5J7.
SMRiQ9C5J7. Positions 27-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21008. 5 interactions.
IntActiQ9C5J7. 6 interactions.
STRINGi3702.AT5G56630.1-P.

Proteomic databases

PaxDbiQ9C5J7.
PRIDEiQ9C5J7.

Protocols and materials databases

DNASUi835764.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630.
GeneIDi835764.
KEGGiath:AT5G56630.

Organism-specific databases

TAIRiAT5G56630.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9C5J7.
KOiK00850.
OMAiIICISEG.
PhylomeDBiQ9C5J7.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciARA:AT5G56630-MONOMER.
ReactomeiREACT_248260. Glycolysis.

Gene expression databases

GenevestigatoriQ9C5J7.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA7_ARATH
AccessioniPrimary (citable) accession number: Q9C5J7
Secondary accession number(s): Q9FJU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.