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Q9C5J7

- PFKA7_ARATH

UniProt

Q9C5J7 - PFKA7_ARATH

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Protein
ATP-dependent 6-phosphofructokinase 7
Gene
PFK7, At5g56630, MIK19.8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by AMP By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATP; via amide nitrogen By similarity
Metal bindingi190 – 1901Magnesium; catalytic By similarity
Sitei191 – 1911Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei220 – 2201Proton acceptor By similarity
Binding sitei319 – 3191Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1652ATP By similarity
Nucleotide bindingi189 – 1924ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G56630-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 7 (EC:2.7.1.11)
Short name:
ATP-PFK 7
Short name:
Phosphofructokinase 7
Alternative name(s):
Phosphohexokinase 7
Gene namesi
Name:PFK7
Ordered Locus Names:At5g56630
ORF Names:MIK19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G56630.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
  2. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485ATP-dependent 6-phosphofructokinase 7UniRule annotation
PRO_0000330774Add
BLAST

Proteomic databases

PaxDbiQ9C5J7.
PRIDEiQ9C5J7.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

GenevestigatoriQ9C5J7.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi21008. 5 interactions.
IntActiQ9C5J7. 6 interactions.
STRINGi3702.AT5G56630.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5J7.
SMRiQ9C5J7. Positions 27-441.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2203Substrate binding By similarity
Regioni263 – 2653Substrate binding By similarity
Regioni374 – 3774Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiQ9C5J7.
KOiK00850.
OMAiSNENGIG.
PhylomeDBiQ9C5J7.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C5J7-1 [UniParc]FASTAAdd to Basket

« Hide

MSSPRSNKPK IVNGPGGYIL QDVPHLIDYL PDLPTYPNPL QDNPAYSVVK    50
QYFVHADDSV PEKVVVHKDG PRGVHFRRAG PRQKVYFESD EVHACIVTCG 100
GLCPGLNTVI REVVSSLSYM YGVKRILGID GGYRGFYAKN TIPLNSKVVN 150
DIHKRGGTII GTSRGGHDTN KIVDSIQDRG INQVYIIGGD GTQRGASVIF 200
EEIRRRRLKV AVVGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA 250
ESNENGIGFV KLMGRYSGYI AMYATLASRD VDCCLIPESP FYLEGEGGLF 300
EFIERRLKDH GHMVIVLAEG AGQDLMCKSM ESTPMDASGN KLLKDVGLWL 350
SQSIKDHFKK NKMVMNLKYI DPTYMIRAVP SNASDNVYCT LLAQSAVHGA 400
MAGYTGYTSG LVNGRQTYIP FYRITETQNN VVITDRMWAR LLSSTNQPSF 450
LGPKDTSEEK KELPETPLLD DGAVDIPPVT KEVTK 485
Length:485
Mass (Da):53,482
Last modified:June 1, 2001 - v1
Checksum:i6F0C6CFF43EAA7B5
GO

Sequence cautioni

The sequence BAB09881.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96790.1.
AF360207 mRNA. Translation: AAK25917.1.
AY040055 mRNA. Translation: AAK64113.1.
RefSeqiNP_568842.1. NM_125046.2.
UniGeneiAt.9267.

Genome annotation databases

EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630.
GeneIDi835764.
KEGGiath:AT5G56630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB013392 Genomic DNA. Translation: BAB09881.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED96790.1 .
AF360207 mRNA. Translation: AAK25917.1 .
AY040055 mRNA. Translation: AAK64113.1 .
RefSeqi NP_568842.1. NM_125046.2.
UniGenei At.9267.

3D structure databases

ProteinModelPortali Q9C5J7.
SMRi Q9C5J7. Positions 27-441.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21008. 5 interactions.
IntActi Q9C5J7. 6 interactions.
STRINGi 3702.AT5G56630.1-P.

Proteomic databases

PaxDbi Q9C5J7.
PRIDEi Q9C5J7.

Protocols and materials databases

DNASUi 835764.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G56630.1 ; AT5G56630.1 ; AT5G56630 .
GeneIDi 835764.
KEGGi ath:AT5G56630.

Organism-specific databases

TAIRi AT5G56630.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000016186.
InParanoidi Q9C5J7.
KOi K00850.
OMAi SNENGIG.
PhylomeDBi Q9C5J7.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci ARA:AT5G56630-MONOMER.

Gene expression databases

Genevestigatori Q9C5J7.

Family and domain databases

HAMAPi MF_01981. Phosphofructokinase_II_X.
InterProi IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFruKinase_TP0108.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana."
    Mustroph A., Sonnewald U., Biemelt S.
    FEBS Lett. 581:2401-2410(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPFKA7_ARATH
AccessioniPrimary (citable) accession number: Q9C5J7
Secondary accession number(s): Q9FJU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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