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Protein

ATP-dependent 6-phosphofructokinase 7

Gene

PFK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FAB6), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATP; via amide nitrogenUniRule annotation1
Metal bindingi190Magnesium; catalyticUniRule annotation1
Sitei191Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei220Proton acceptorUniRule annotation1
Binding sitei319SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 165ATPUniRule annotation2
Nucleotide bindingi189 – 192ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G56630-MONOMER.
BRENDAi2.7.1.11. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 7UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 7UniRule annotation
Short name:
Phosphofructokinase 7UniRule annotation
Alternative name(s):
Phosphohexokinase 7UniRule annotation
Gene namesi
Name:PFK7UniRule annotation
Ordered Locus Names:At5g56630
ORF Names:MIK19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G56630.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003307741 – 485ATP-dependent 6-phosphofructokinase 7Add BLAST485

Proteomic databases

PaxDbiQ9C5J7.
PRIDEiQ9C5J7.

PTM databases

iPTMnetiQ9C5J7.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

GenevisibleiQ9C5J7. AT.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi21008. 5 interactors.
IntActiQ9C5J7. 6 interactors.
STRINGi3702.AT5G56630.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5J7.
SMRiQ9C5J7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 220Substrate bindingUniRule annotation3
Regioni263 – 265Substrate bindingUniRule annotation3
Regioni374 – 377Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000016186.
InParanoidiQ9C5J7.
KOiK00850.
OMAiITETQNN.
OrthoDBiEOG093609PL.
PhylomeDBiQ9C5J7.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9C5J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPRSNKPK IVNGPGGYIL QDVPHLIDYL PDLPTYPNPL QDNPAYSVVK
60 70 80 90 100
QYFVHADDSV PEKVVVHKDG PRGVHFRRAG PRQKVYFESD EVHACIVTCG
110 120 130 140 150
GLCPGLNTVI REVVSSLSYM YGVKRILGID GGYRGFYAKN TIPLNSKVVN
160 170 180 190 200
DIHKRGGTII GTSRGGHDTN KIVDSIQDRG INQVYIIGGD GTQRGASVIF
210 220 230 240 250
EEIRRRRLKV AVVGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA
260 270 280 290 300
ESNENGIGFV KLMGRYSGYI AMYATLASRD VDCCLIPESP FYLEGEGGLF
310 320 330 340 350
EFIERRLKDH GHMVIVLAEG AGQDLMCKSM ESTPMDASGN KLLKDVGLWL
360 370 380 390 400
SQSIKDHFKK NKMVMNLKYI DPTYMIRAVP SNASDNVYCT LLAQSAVHGA
410 420 430 440 450
MAGYTGYTSG LVNGRQTYIP FYRITETQNN VVITDRMWAR LLSSTNQPSF
460 470 480
LGPKDTSEEK KELPETPLLD DGAVDIPPVT KEVTK
Length:485
Mass (Da):53,482
Last modified:June 1, 2001 - v1
Checksum:i6F0C6CFF43EAA7B5
GO

Sequence cautioni

The sequence BAB09881 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96790.1.
AF360207 mRNA. Translation: AAK25917.1.
AY040055 mRNA. Translation: AAK64113.1.
RefSeqiNP_568842.1. NM_125046.3.
UniGeneiAt.9267.

Genome annotation databases

EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630.
GeneIDi835764.
GrameneiAT5G56630.1; AT5G56630.1; AT5G56630.
KEGGiath:AT5G56630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA. Translation: BAB09881.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96790.1.
AF360207 mRNA. Translation: AAK25917.1.
AY040055 mRNA. Translation: AAK64113.1.
RefSeqiNP_568842.1. NM_125046.3.
UniGeneiAt.9267.

3D structure databases

ProteinModelPortaliQ9C5J7.
SMRiQ9C5J7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21008. 5 interactors.
IntActiQ9C5J7. 6 interactors.
STRINGi3702.AT5G56630.1.

PTM databases

iPTMnetiQ9C5J7.

Proteomic databases

PaxDbiQ9C5J7.
PRIDEiQ9C5J7.

Protocols and materials databases

DNASUi835764.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630.
GeneIDi835764.
GrameneiAT5G56630.1; AT5G56630.1; AT5G56630.
KEGGiath:AT5G56630.

Organism-specific databases

TAIRiAT5G56630.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000016186.
InParanoidiQ9C5J7.
KOiK00850.
OMAiITETQNN.
OrthoDBiEOG093609PL.
PhylomeDBiQ9C5J7.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciARA:AT5G56630-MONOMER.
BRENDAi2.7.1.11. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.

Miscellaneous databases

PROiQ9C5J7.

Gene expression databases

GenevisibleiQ9C5J7. AT.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA7_ARATH
AccessioniPrimary (citable) accession number: Q9C5J7
Secondary accession number(s): Q9FJU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.