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Protein

ATP-dependent 6-phosphofructokinase 7

Gene

PFK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (MJB21.12), Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (FBA6), Fructose-bisphosphate aldolase (FBA4), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FBA6), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (FBA7), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101ATP; via amide nitrogenUniRule annotation1
Metal bindingi190Magnesium; catalyticUniRule annotation1
Sitei191Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei220Proton acceptorUniRule annotation1
Binding sitei319SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 165ATPUniRule annotation2
Nucleotide bindingi189 – 192ATPUniRule annotation4

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G56630-MONOMER
BRENDAi2.7.1.11 399
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 7UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 7UniRule annotation
Short name:
Phosphofructokinase 7UniRule annotation
Alternative name(s):
Phosphohexokinase 7UniRule annotation
Gene namesi
Name:PFK7UniRule annotation
Ordered Locus Names:At5g56630
ORF Names:MIK19.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56630
TAIRilocus:2165046 AT5G56630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003307741 – 485ATP-dependent 6-phosphofructokinase 7Add BLAST485

Proteomic databases

PaxDbiQ9C5J7
PRIDEiQ9C5J7

PTM databases

iPTMnetiQ9C5J7

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9C5J7 baseline and differential
GenevisibleiQ9C5J7 AT

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi21008, 5 interactors
IntActiQ9C5J7, 6 interactors
STRINGi3702.AT5G56630.1

Structurei

3D structure databases

ProteinModelPortaliQ9C5J7
SMRiQ9C5J7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 220Substrate bindingUniRule annotation3
Regioni263 – 265Substrate bindingUniRule annotation3
Regioni374 – 377Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440 Eukaryota
COG0205 LUCA
HOGENOMiHOG000016186
InParanoidiQ9C5J7
KOiK00850
OMAiITETQNN
OrthoDBiEOG093609PL
PhylomeDBiQ9C5J7

Family and domain databases

HAMAPiMF_01981 Phosphofructokinase_II_X, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR012004 PyroP-dep_PFK_TP0108
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000534 PPi_PFK_TP0108, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

Q9C5J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPRSNKPK IVNGPGGYIL QDVPHLIDYL PDLPTYPNPL QDNPAYSVVK
60 70 80 90 100
QYFVHADDSV PEKVVVHKDG PRGVHFRRAG PRQKVYFESD EVHACIVTCG
110 120 130 140 150
GLCPGLNTVI REVVSSLSYM YGVKRILGID GGYRGFYAKN TIPLNSKVVN
160 170 180 190 200
DIHKRGGTII GTSRGGHDTN KIVDSIQDRG INQVYIIGGD GTQRGASVIF
210 220 230 240 250
EEIRRRRLKV AVVGIPKTID NDIPVIDKSF GFDTAVEEAQ RAINAAHVEA
260 270 280 290 300
ESNENGIGFV KLMGRYSGYI AMYATLASRD VDCCLIPESP FYLEGEGGLF
310 320 330 340 350
EFIERRLKDH GHMVIVLAEG AGQDLMCKSM ESTPMDASGN KLLKDVGLWL
360 370 380 390 400
SQSIKDHFKK NKMVMNLKYI DPTYMIRAVP SNASDNVYCT LLAQSAVHGA
410 420 430 440 450
MAGYTGYTSG LVNGRQTYIP FYRITETQNN VVITDRMWAR LLSSTNQPSF
460 470 480
LGPKDTSEEK KELPETPLLD DGAVDIPPVT KEVTK
Length:485
Mass (Da):53,482
Last modified:June 1, 2001 - v1
Checksum:i6F0C6CFF43EAA7B5
GO

Sequence cautioni

The sequence BAB09881 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013392 Genomic DNA Translation: BAB09881.1 Sequence problems.
CP002688 Genomic DNA Translation: AED96790.1
AF360207 mRNA Translation: AAK25917.1
AY040055 mRNA Translation: AAK64113.1
RefSeqiNP_568842.1, NM_125046.3
UniGeneiAt.9267

Genome annotation databases

EnsemblPlantsiAT5G56630.1; AT5G56630.1; AT5G56630
GeneIDi835764
GrameneiAT5G56630.1; AT5G56630.1; AT5G56630
KEGGiath:AT5G56630

Similar proteinsi

Entry informationi

Entry nameiPFKA7_ARATH
AccessioniPrimary (citable) accession number: Q9C5J7
Secondary accession number(s): Q9FJU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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