ID   USP_ARATH               Reviewed;         614 AA.
AC   Q9C5I1; Q9LTG1;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   16-JUN-2009, entry version 39.
DE   RecName: Full=UDP-sugar pyrophosphorylase;
DE            Short=AtUSP;
DE            EC=2.7.7.64;
GN   Name=USP; OrderedLocusNames=At5g52560; ORFNames=F6N7.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Bar-Peled L., Bar-Peled M.;
RT   "Biochemical characterization of a nonspecific UDP-sugar
RT   pyrophosphorylase from Arabidopsis.";
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=16757173; DOI=10.1016/j.plaphy.2006.04.004;
RA   Litterer L.A., Schnurr J.A., Plaisance K.L., Storey K.K.,
RA   Gronwald J.W., Somers D.A.;
RT   "Characterization and expression of Arabidopsis UDP-sugar
RT   pyrophosphorylase.";
RL   Plant Physiol. Biochem. 44:171-180(2006).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16557401; DOI=10.1007/s00425-006-0240-1;
RA   Schnurr J.A., Storey K.K., Jung H.-J.G., Somers D.A., Gronwald J.W.;
RT   "UDP-sugar pyrophosphorylase is essential for pollen development in
RT   Arabidopsis.";
RL   Planta 224:520-532(2006).
CC   -!- FUNCTION: Required for the synthesis of the intine, the
CC       pectocellulosic inner wall of developing pollen. May function as
CC       the terminal enzyme of the myo-inositol oxidation (MIO) pathway.
CC       May also play a role in the salvage pathway for synthesis of
CC       nucleotide sugars.
CC   -!- CATALYTIC ACTIVITY: UTP + a monosaccharide 1-phosphate =
CC       diphosphate + UDP-monosaccharide.
CC   -!- COFACTOR: Magnesium or manganese.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.13 mM for glucuronic acid-1-phosphate;
CC         KM=0.42 mM for glucose-1-phosphate;
CC         KM=0.16 mM for UTP;
CC         KM=0.56 mM for UDP-glucuronic acid;
CC         KM=0.72 mM for UPD-glucose;
CC         KM=0.15 mM for pyrophosphate;
CC         Vmax=3.47 nmol/min/mg enzyme for the forward reaction with
CC         glucuronic acid-1-phosphate as substrate;
CC         Vmax=5.76 nmol/min/mg enzyme for the forward reaction with
CC         glucose-1-phosphate as substrate;
CC         Vmax=4.24 nmol/min/mg enzyme for the reverse reaction with UDP-
CC         glucuronic acid as substrate;
CC         Vmax=4.95 nmol/min/mg enzyme for the reverse reaction with UDP-
CC         glucose as substrate;
CC         Note=High activity with glucose-1-phosphate > glucuronic acid-1-
CC         phosphate > galactose-1-phosphate, but low or no activity with
CC         N-acetylglucosamine-1-phosphate, fucose-1-phosphate, mannose-1-
CC         phosphate, inositol-1-phosphate or glucose-6-phosphate;
CC       pH dependence:
CC         Optimum pH is 7.5-8.5. Inactive at or below pH 5.0;
CC       Temperature dependence:
CC         Optimum temperature is 45 degrees Celsius;
CC   -!- TISSUE SPECIFICITY: Ubiquitous, but most abundant in rosette
CC       leaves, inflorescences, stems, stamens and pollen.
CC   -!- DEVELOPMENTAL STAGE: Expressed during pollen development, peaking
CC       at the tricellular stage.
CC   -!- DISRUPTION PHENOTYPE: Plants produce collapsed, nonviable pollen
CC       grains.
CC   -!- SIMILARITY: Belongs to the USP family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA98074.1; Type=Erroneous gene model prediction;
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DR   EMBL; DQ324529; ABC55066.1; -; mRNA.
DR   EMBL; AB025606; BAA98074.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AF360236; AAK25946.1; -; mRNA.
DR   EMBL; AY040035; AAK64093.1; -; mRNA.
DR   IPI; IPI00524775; -.
DR   RefSeq; NP_568775.1; -.
DR   UniGene; At.49163; -.
DR   PRIDE; Q9C5I1; -.
DR   GeneID; 835333; -.
DR   GenomeReviews; BA000015_GR; AT5G52560.
DR   KEGG; ath:AT5G52560; -.
DR   NMPDR; fig|3702.1.peg.27140; -.
DR   TAIR; At5g52560; -.
DR   OMA; Q9C5I1; MTSDDTH.
DR   BRENDA; 2.7.7.64; 302.
DR   ArrayExpress; Q9C5I1; -.
DR   GO; GO:0047350; F:glucuronate-1-phosphate uridylyltransferase...; IDA:TAIR.
DR   GO; GO:0010491; F:UTP:arabinose-1-phosphate uridylyltransfera...; IDA:TAIR.
DR   GO; GO:0017103; F:UTP:galactose-1-phosphate uridylyltransfera...; IDA:TAIR.
DR   GO; GO:0003983; F:UTP:glucose-1-phosphate uridylyltransferase...; IDA:TAIR.
DR   GO; GO:0047338; F:UTP:xylose-1-phosphate uridylyltransferase ...; IDA:TAIR.
DR   GO; GO:0008152; P:metabolic process; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEP:TAIR.
DR   InterPro; IPR002618; UDPGP_trans.
DR   PANTHER; PTHR11952; UDPGP_trans; 1.
DR   Pfam; PF01704; UDPGP; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Nucleotidyltransferase; Transferase.
FT   CHAIN         1    614       UDP-sugar pyrophosphorylase.
FT                                /FTId=PRO_0000289978.
SQ   SEQUENCE   614 AA;  67852 MW;  A83D823026E1BDA8 CRC64;
     MASTVDSNFF SSVPALHSNL GLLSPDQIEL AKILLENGQS HLFQQWPELG VDDKEKLAFF
     DQIARLNSSY PGGLAAYIKT AKELLADSKV GKNPYDGFSP SVPSGENLTF GTDNFIEMEK
     RGVVEARNAA FVLVAGGLGE RLGYNGIKVA LPRETTTGTC FLQHYIESIL ALQEASNKID
     SDGSERDIPF IIMTSDDTHS RTLDLLELNS YFGMKPTQVH LLKQEKVACL DDNDARLALD
     PHNKYSIQTK PHGHGDVHSL LYSSGLLHKW LEAGLKWVLF FQDTNGLLFN AIPASLGVSA
     TKQYHVNSLA VPRKAKEAIG GISKLTHVDG RSMVINVEYN QLDPLLRASG FPDGDVNCET
     GFSPFPGNIN QLILELGPYK DELQKTGGAI KEFVNPKYKD STKTAFKSST RLECMMQDYP
     KTLPPTARVG FTVMDIWLAY APVKNNPEDA AKVPKGNPYH SATSGEMAIY RANSLILQKA
     GVKVEEPVKQ VLNGQEVEVW SRITWKPKWG MIFSDIKKKV SGNCEVSQRS TMAIKGRNVF
     IKDLSLDGAL IVDSIDDAEV KLGGLIKNNG WTMESVDYKD TSVPEEIRIR GFRFNKVEQL
     EKKLTQPGKF SVED
//