Reviewed, UniProtKB/Swiss-Prot Q9C5I1 (USP_ARATH)

Names and origin

Protein names

Recommended name:
    UDP-sugar pyrophosphorylase
      Short name=AtUSP
    EC=2.7.7.64

Gene names

Name:	USP
Ordered Locus Names:	At5g52560
ORF Names:	F6N7.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the synthesis of the intine, the pectocellulosic inner wall of developing pollen. May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Ref.4 Ref.5
Catalytic activity	UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.
Cofactor	Magnesium or manganese.
Tissue specificity	Ubiquitous, but most abundant in rosette leaves, inflorescences, stems, stamens and pollen. Ref.4
Developmental stage	Expressed during pollen development, peaking at the tricellular stage. Ref.4
Disruption phenotype	Plants produce collapsed, nonviable pollen grains. Ref.5
Sequence similarities	Belongs to the USP family.
Biophysicochemical properties	Kinetic parameters: High activity with glucose-1-phosphate > glucuronic acid-1-phosphate > galactose-1-phosphate, but low or no activity with N-acetylglucosamine-1-phosphate, fucose-1-phosphate, mannose-1-phosphate, inositol-1-phosphate or glucose-6-phosphate. KM=0.13 mM for glucuronic acid-1-phosphate KM=0.42 mM for glucose-1-phosphate KM=0.16 mM for UTP KM=0.56 mM for UDP-glucuronic acid KM=0.72 mM for UPD-glucose KM=0.15 mM for pyrophosphate Vmax=3.47 nmol/min/mg enzyme for the forward reaction with glucuronic acid-1-phosphate as substrate Vmax=5.76 nmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate Vmax=4.24 nmol/min/mg enzyme for the reverse reaction with UDP-glucuronic acid as substrate Vmax=4.95 nmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate pH dependence: Optimum pH is 7.5-8.5. Inactive at or below pH 5.0. Temperature dependence: Optimum temperature is 45 degrees Celsius.
Sequence caution	The sequence BAA98074.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from expression pattern. Source: TAIR
Molecular function	UTP:arabinose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR UTP:galactose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR UTP:glucose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR UTP:xylose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR glucuronate-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	UDP-sugar pyrophosphorylase		PRO_0000289978

Sequences

	Sequence		Length	Mass (Da)
	Q9C5I1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A83D823026E1BDA8	FASTA	614	67,852
	10 20 30 40 50 60 MASTVDSNFF SSVPALHSNL GLLSPDQIEL AKILLENGQS HLFQQWPELG VDDKEKLAFF 70 80 90 100 110 120 DQIARLNSSY PGGLAAYIKT AKELLADSKV GKNPYDGFSP SVPSGENLTF GTDNFIEMEK 130 140 150 160 170 180 RGVVEARNAA FVLVAGGLGE RLGYNGIKVA LPRETTTGTC FLQHYIESIL ALQEASNKID 190 200 210 220 230 240 SDGSERDIPF IIMTSDDTHS RTLDLLELNS YFGMKPTQVH LLKQEKVACL DDNDARLALD 250 260 270 280 290 300 PHNKYSIQTK PHGHGDVHSL LYSSGLLHKW LEAGLKWVLF FQDTNGLLFN AIPASLGVSA 310 320 330 340 350 360 TKQYHVNSLA VPRKAKEAIG GISKLTHVDG RSMVINVEYN QLDPLLRASG FPDGDVNCET 370 380 390 400 410 420 GFSPFPGNIN QLILELGPYK DELQKTGGAI KEFVNPKYKD STKTAFKSST RLECMMQDYP 430 440 450 460 470 480 KTLPPTARVG FTVMDIWLAY APVKNNPEDA AKVPKGNPYH SATSGEMAIY RANSLILQKA 490 500 510 520 530 540 GVKVEEPVKQ VLNGQEVEVW SRITWKPKWG MIFSDIKKKV SGNCEVSQRS TMAIKGRNVF 550 560 570 580 590 600 IKDLSLDGAL IVDSIDDAEV KLGGLIKNNG WTMESVDYKD TSVPEEIRIR GFRFNKVEQL 610 EKKLTQPGKF SVED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical characterization of a nonspecific UDP-sugar pyrophosphorylase from Arabidopsis." Bar-Peled L., Bar-Peled M. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Characterization and expression of Arabidopsis UDP-sugar pyrophosphorylase." Litterer L.A., Schnurr J.A., Plaisance K.L., Storey K.K., Gronwald J.W., Somers D.A. Plant Physiol. Biochem. 44:171-180(2006) [PubMed: 16757173] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"UDP-sugar pyrophosphorylase is essential for pollen development in Arabidopsis." Schnurr J.A., Storey K.K., Jung H.-J.G., Somers D.A., Gronwald J.W. Planta 224:520-532(2006) [PubMed: 16557401] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ324529 mRNA. Translation: ABC55066.1. AB025606 Genomic DNA. Translation: BAA98074.1. Sequence problems. AF360236 mRNA. Translation: AAK25946.1. AY040035 mRNA. Translation: AAK64093.1.
IPI	IPI00524775.
RefSeq	NP_568775.1.
UniGene	At.49163
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9C5I1.
Genome annotation databases
GeneID	835333.
GenomeReviews	Gene locus AT5G52560 in contig BA000015_GR.
KEGG	ath:AT5G52560.
NMPDR	fig|3702.1.peg.27140.
Organism-specific databases
TAIR	At5g52560.
Phylogenomic databases
OMA	Q9C5I1. MTSDDTH.
Enzyme and pathway databases
BRENDA	2.7.7.64. 302.
Gene expression databases
ArrayExpress	Q9C5I1.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. [Graphical view]
PANTHER	PTHR11952. UDPGP_trans. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

USP_ARATH

Accession

Primary (citable) accession number: Q9C5I1
Secondary accession number(s): Q9LTG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)