UDP-sugar pyrophosphorylase - Arabidopsis thaliana (Mouse-ear cress)

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Q9C5I1 (USP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 58. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UDP-sugar pyrophosphorylase
Short name=AtUSP
EC=2.7.7.64

Gene names

Name:	USP
Ordered Locus Names:	At5g52560
ORF Names:	F6N7.4

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the synthesis of the intine, the pectocellulosic inner wall of developing pollen. May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Ref.5 Ref.6
Catalytic activity	UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.
Cofactor	Magnesium or manganese.
Tissue specificity	Ubiquitous, but most abundant in rosette leaves, inflorescences, stems, stamens and pollen. Ref.5
Developmental stage	Expressed during pollen development, peaking at the tricellular stage. Ref.5
Disruption phenotype	Plants produce collapsed, nonviable pollen grains. Ref.6
Sequence similarities	Belongs to the USP family.
Biophysicochemical properties	Kinetic parameters: High activity with glucose-1-phosphate > glucuronic acid-1-phosphate > galactose-1-phosphate, but low or no activity with N-acetylglucosamine-1-phosphate, fucose-1-phosphate, mannose-1-phosphate, inositol-1-phosphate or glucose-6-phosphate. K_M=0.13 mM for glucuronic acid-1-phosphate Ref.5 K_M=0.42 mM for glucose-1-phosphate K_M=0.16 mM for UTP K_M=0.56 mM for UDP-glucuronic acid K_M=0.72 mM for UPD-glucose K_M=0.15 mM for pyrophosphate V_max=3.47 nmol/min/mg enzyme for the forward reaction with glucuronic acid-1-phosphate as substrate V_max=5.76 nmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate V_max=4.24 nmol/min/mg enzyme for the reverse reaction with UDP-glucuronic acid as substrate V_max=4.95 nmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate pH dependence: Optimum pH is 7.5-8.5. Inactive at or below pH 5.0. Temperature dependence: Optimum temperature is 45 degrees Celsius.
Sequence caution	The sequence BAA98074.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	UDP-D-galactose metabolic process Inferred from direct assay Ref.5. Source: TAIR UDP-L-arabinose metabolic process Inferred from mutant phenotype. Source: TAIR UDP-glucose metabolic process Inferred from direct assay Ref.5. Source: TAIR UDP-glucuronate metabolic process Inferred from direct assay Ref.5. Source: TAIR pollen development Inferred from mutant phenotype. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR pollen tube Inferred from direct assay. Source: TAIR
Molecular function	UTP:arabinose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR UTP:galactose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR UTP:glucose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR UTP:xylose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR glucuronate-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 614

614

UDP-sugar pyrophosphorylase

PRO_0000289978

Sequences

Sequence

Length

Mass (Da)

Tools

Q9C5I1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: A83D823026E1BDA8
Show »

FASTA

614

67,852

        10         20         30         40         50         60 
MASTVDSNFF SSVPALHSNL GLLSPDQIEL AKILLENGQS HLFQQWPELG VDDKEKLAFF 

        70         80         90        100        110        120 
DQIARLNSSY PGGLAAYIKT AKELLADSKV GKNPYDGFSP SVPSGENLTF GTDNFIEMEK 

       130        140        150        160        170        180 
RGVVEARNAA FVLVAGGLGE RLGYNGIKVA LPRETTTGTC FLQHYIESIL ALQEASNKID 

       190        200        210        220        230        240 
SDGSERDIPF IIMTSDDTHS RTLDLLELNS YFGMKPTQVH LLKQEKVACL DDNDARLALD 

       250        260        270        280        290        300 
PHNKYSIQTK PHGHGDVHSL LYSSGLLHKW LEAGLKWVLF FQDTNGLLFN AIPASLGVSA 

       310        320        330        340        350        360 
TKQYHVNSLA VPRKAKEAIG GISKLTHVDG RSMVINVEYN QLDPLLRASG FPDGDVNCET 

       370        380        390        400        410        420 
GFSPFPGNIN QLILELGPYK DELQKTGGAI KEFVNPKYKD STKTAFKSST RLECMMQDYP 

       430        440        450        460        470        480 
KTLPPTARVG FTVMDIWLAY APVKNNPEDA AKVPKGNPYH SATSGEMAIY RANSLILQKA 

       490        500        510        520        530        540 
GVKVEEPVKQ VLNGQEVEVW SRITWKPKWG MIFSDIKKKV SGNCEVSQRS TMAIKGRNVF 

       550        560        570        580        590        600 
IKDLSLDGAL IVDSIDDAEV KLGGLIKNNG WTMESVDYKD TSVPEEIRIR GFRFNKVEQL 

       610 
EKKLTQPGKF SVED

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical characterization of a nonspecific UDP-sugar pyrophosphorylase from Arabidopsis." Bar-Peled L., Bar-Peled M. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Characterization and expression of Arabidopsis UDP-sugar pyrophosphorylase." Litterer L.A., Schnurr J.A., Plaisance K.L., Storey K.K., Gronwald J.W., Somers D.A. Plant Physiol. Biochem. 44:171-180(2006) [PubMed: 16757173] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"UDP-sugar pyrophosphorylase is essential for pollen development in Arabidopsis." Schnurr J.A., Storey K.K., Jung H.-J.G., Somers D.A., Gronwald J.W. Planta 224:520-532(2006) [PubMed: 16557401] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ324529 mRNA. Translation: ABC55066.1. AB025606 Genomic DNA. Translation: BAA98074.1. Sequence problems. CP002688 Genomic DNA. Translation: AED96234.1. AF360236 mRNA. Translation: AAK25946.1. AY040035 mRNA. Translation: AAK64093.1.
IPI	IPI00524775.
RefSeq	NP_568775.1. NM_124635.3.
UniGene	At.19016. At.49163.
3D structure databases
ProteinModelPortal	Q9C5I1.
SMR	Q9C5I1. Positions 25-605.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C5I1.
Proteomic databases
PRIDE	Q9C5I1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G52560.1; AT5G52560.1; AT5G52560.
GeneID	835333.
GenomeReviews	Gene locus AT5G52560 in contig BA000015_GR.
KEGG	ath:AT5G52560.
NMPDR	fig\|3702.1.peg.27140.
Organism-specific databases
TAIR	At5g52560.
Phylogenomic databases
eggNOG	KOG2388.
GeneTree	EPGT00070000030731.
HOGENOM	HBG521338.
InParanoid	Q9C5I1.
OMA	PFPGNIN.
PhylomeDB	Q9C5I1.
ProtClustDB	PLN02830.
Gene expression databases
ArrayExpress	Q9C5I1.
Genevestigator	Q9C5I1.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. [Graphical view]
KO	K12447.
PANTHER	PTHR11952. UDPGP_trans. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

USP_ARATH

Accession

Primary (citable) accession number: Q9C5I1
Secondary accession number(s): Q9LTG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents