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Q9C5E5

- DGK5_ARATH

UniProt

Q9C5E5 - DGK5_ARATH

Protein

Diacylglycerol kinase 5

Gene

DGK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Plant defense, Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G20900-MONOMER.
    ARA:GQT-2023-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase 5 (EC:2.7.1.107)
    Short name:
    AtDGK5
    Short name:
    DAG kinase 5
    Alternative name(s):
    Diglyceride kinase 5
    Short name:
    DGK 5
    Gene namesi
    Name:DGK5
    Ordered Locus Names:At2g20900
    ORF Names:F5H14.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G20900.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 509509Diacylglycerol kinase 5PRO_0000422113Add
    BLAST

    Proteomic databases

    PaxDbiQ8LE70.
    PRIDEiQ9C5E5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9C5E5.
    GenevestigatoriQ9C5E5.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    IntActiQ9C5E5. 1 interaction.
    STRINGi3702.AT2G20900.4-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5E5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 187152DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi24 – 329Poly-Glu

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000241061.
    InParanoidiQ9C5E5.
    KOiK00901.
    OMAiATHDCRS.
    PhylomeDBiQ9C5E5.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR016961. Diacylglycerol_kinase_pln.
    [Graphical view]
    PfamiPF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
    SMARTiSM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9C5E5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKYNSLSDF LKEFYIPTYV LSAETEEEEE EESRPTPASP VLVFINSKSG    50
    GQLGGELILT YRSLLNHNQV FDLDQETPDK VLRRIYLNLE RLKDDDFARQ 100
    IREKLKIIVA GGDGTAGWLL GVVCDLKLSH PPPIATVPLG TGNNLPFAFG 150
    WGKKNPGTDR TAVESFLEQV LKAKVMKIDN WHILMRMKTP KEGGSCDPVA 200
    PLELPHSLHA FHRVSPTDEL NKEGCHTFRG GFWNYFSLGM DAQISYAFHS 250
    ERKLHPEKFK NQLVNQSTYV KLGCTQGWFC ASLFHPASRN IAQLAKVKIA 300
    TRNGQWQDLH IPHSIRSIVC LNLPSFSGGL NPWGTPNPRK QRDRGLTPPF 350
    VDDGLIEVVG FRNAWHGLVL LAPNGHGTRL AQANRIRFEF HKGATDHTFM 400
    RMDGEPWKQP LPLDDETVMV EISHLGQVNM LATHDCRSRS VFDPSTPRHQ 450
    DGAEDYDDNE DDSVAEGEEF RKFGAADTFK IPDEGEHSNK KGRASRRRNS 500
    NVHGWSHVL 509
    Length:509
    Mass (Da):57,377
    Last modified:June 1, 2001 - v1
    Checksum:iF6F9A08630D327DA
    GO
    Isoform 2 (identifier: Q9C5E5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         485-491: GEHSNKK → VDISQLS
         492-509: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:491
    Mass (Da):55,267
    Checksum:i117384E426570568
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311E → Q in AAM62810. 1 PublicationCurated
    Sequence conflicti97 – 971F → S in AAM62810. 1 PublicationCurated
    Sequence conflicti102 – 1021R → G in AAM62810. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei485 – 4917GEHSNKK → VDISQLS in isoform 2. 2 PublicationsVSP_046410
    Alternative sequencei492 – 50918Missing in isoform 2. 2 PublicationsVSP_046411Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006234 Genomic DNA. Translation: AAD20931.2.
    CP002685 Genomic DNA. Translation: AEC07093.1.
    CP002685 Genomic DNA. Translation: AEC07094.1.
    CP002685 Genomic DNA. Translation: AEC07095.1.
    CP002685 Genomic DNA. Translation: AEC07096.1.
    AF360300 mRNA. Translation: AAK26010.1.
    BT000983 mRNA. Translation: AAN41383.1.
    AK316877 mRNA. Translation: BAH19585.1.
    AK317470 mRNA. Translation: BAH20135.1.
    AY085589 mRNA. Translation: AAM62810.1.
    PIRiG84594.
    RefSeqiNP_001031381.1. NM_001036304.1. [Q9C5E5-1]
    NP_565492.1. NM_127660.3. [Q9C5E5-2]
    NP_850007.1. NM_179676.2. [Q9C5E5-1]
    NP_973498.1. NM_201769.2. [Q9C5E5-2]
    UniGeneiAt.46381.
    At.48498.

    Genome annotation databases

    EnsemblPlantsiAT2G20900.1; AT2G20900.1; AT2G20900. [Q9C5E5-1]
    AT2G20900.4; AT2G20900.4; AT2G20900. [Q9C5E5-1]
    GeneIDi816624.
    KEGGiath:AT2G20900.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006234 Genomic DNA. Translation: AAD20931.2 .
    CP002685 Genomic DNA. Translation: AEC07093.1 .
    CP002685 Genomic DNA. Translation: AEC07094.1 .
    CP002685 Genomic DNA. Translation: AEC07095.1 .
    CP002685 Genomic DNA. Translation: AEC07096.1 .
    AF360300 mRNA. Translation: AAK26010.1 .
    BT000983 mRNA. Translation: AAN41383.1 .
    AK316877 mRNA. Translation: BAH19585.1 .
    AK317470 mRNA. Translation: BAH20135.1 .
    AY085589 mRNA. Translation: AAM62810.1 .
    PIRi G84594.
    RefSeqi NP_001031381.1. NM_001036304.1. [Q9C5E5-1 ]
    NP_565492.1. NM_127660.3. [Q9C5E5-2 ]
    NP_850007.1. NM_179676.2. [Q9C5E5-1 ]
    NP_973498.1. NM_201769.2. [Q9C5E5-2 ]
    UniGenei At.46381.
    At.48498.

    3D structure databases

    ProteinModelPortali Q9C5E5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9C5E5. 1 interaction.
    STRINGi 3702.AT2G20900.4-P.

    Proteomic databases

    PaxDbi Q8LE70.
    PRIDEi Q9C5E5.

    Protocols and materials databases

    DNASUi 816624.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G20900.1 ; AT2G20900.1 ; AT2G20900 . [Q9C5E5-1 ]
    AT2G20900.4 ; AT2G20900.4 ; AT2G20900 . [Q9C5E5-1 ]
    GeneIDi 816624.
    KEGGi ath:AT2G20900.

    Organism-specific databases

    TAIRi AT2G20900.

    Phylogenomic databases

    HOGENOMi HOG000241061.
    InParanoidi Q9C5E5.
    KOi K00901.
    OMAi ATHDCRS.
    PhylomeDBi Q9C5E5.

    Enzyme and pathway databases

    BioCyci ARA:AT2G20900-MONOMER.
    ARA:GQT-2023-MONOMER.

    Gene expression databases

    ArrayExpressi Q9C5E5.
    Genevestigatori Q9C5E5.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR016961. Diacylglycerol_kinase_pln.
    [Graphical view ]
    Pfami PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
    SMARTi SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

    Entry informationi

    Entry nameiDGK5_ARATH
    AccessioniPrimary (citable) accession number: Q9C5E5
    Secondary accession number(s): Q8LE70, Q9SKS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3