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Protein

Diacylglycerol kinase 5

Gene

DGK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPlant defense, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G20900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 5 (EC:2.7.1.107)
Short name:
AtDGK5
Short name:
DAG kinase 5
Alternative name(s):
Diglyceride kinase 5
Short name:
DGK 5
Gene namesi
Name:DGK5
Ordered Locus Names:At2g20900
ORF Names:F5H14.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G20900.
TAIRilocus:2051343. AT2G20900.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221131 – 509Diacylglycerol kinase 5Add BLAST509

Proteomic databases

PaxDbiQ9C5E5.

PTM databases

iPTMnetiQ9C5E5.

Expressioni

Gene expression databases

GenevisibleiQ9C5E5. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi1977. 1 interactor.
IntActiQ9C5E5. 1 interactor.
STRINGi3702.AT2G20900.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5E5.
SMRiQ9C5E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 187DAGKcPROSITE-ProRule annotationAdd BLAST152

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 32Poly-Glu9

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
HOGENOMiHOG000241061.
InParanoidiQ9C5E5.
KOiK00901.
OMAiKIMKRAG.
OrthoDBiEOG093608DB.
PhylomeDBiQ9C5E5.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiView protein in InterPro
IPR017438. ATP-NAD_kinase_dom_1.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
IPR016064. NAD/diacylglycerol_kinase.
PfamiView protein in Pfam
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTiView protein in SMART
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiView protein in PROSITE
PS50146. DAGK. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C5E5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKYNSLSDF LKEFYIPTYV LSAETEEEEE EESRPTPASP VLVFINSKSG
60 70 80 90 100
GQLGGELILT YRSLLNHNQV FDLDQETPDK VLRRIYLNLE RLKDDDFARQ
110 120 130 140 150
IREKLKIIVA GGDGTAGWLL GVVCDLKLSH PPPIATVPLG TGNNLPFAFG
160 170 180 190 200
WGKKNPGTDR TAVESFLEQV LKAKVMKIDN WHILMRMKTP KEGGSCDPVA
210 220 230 240 250
PLELPHSLHA FHRVSPTDEL NKEGCHTFRG GFWNYFSLGM DAQISYAFHS
260 270 280 290 300
ERKLHPEKFK NQLVNQSTYV KLGCTQGWFC ASLFHPASRN IAQLAKVKIA
310 320 330 340 350
TRNGQWQDLH IPHSIRSIVC LNLPSFSGGL NPWGTPNPRK QRDRGLTPPF
360 370 380 390 400
VDDGLIEVVG FRNAWHGLVL LAPNGHGTRL AQANRIRFEF HKGATDHTFM
410 420 430 440 450
RMDGEPWKQP LPLDDETVMV EISHLGQVNM LATHDCRSRS VFDPSTPRHQ
460 470 480 490 500
DGAEDYDDNE DDSVAEGEEF RKFGAADTFK IPDEGEHSNK KGRASRRRNS

NVHGWSHVL
Length:509
Mass (Da):57,377
Last modified:June 1, 2001 - v1
Checksum:iF6F9A08630D327DA
GO
Isoform 2 (identifier: Q9C5E5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     485-491: GEHSNKK → VDISQLS
     492-509: Missing.

Note: No experimental confirmation available.
Show »
Length:491
Mass (Da):55,267
Checksum:i117384E426570568
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31E → Q in AAM62810 (Ref. 5) Curated1
Sequence conflicti97F → S in AAM62810 (Ref. 5) Curated1
Sequence conflicti102R → G in AAM62810 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046410485 – 491GEHSNKK → VDISQLS in isoform 2. 2 Publications7
Alternative sequenceiVSP_046411492 – 509Missing in isoform 2. 2 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006234 Genomic DNA. Translation: AAD20931.2.
CP002685 Genomic DNA. Translation: AEC07093.1.
CP002685 Genomic DNA. Translation: AEC07094.1.
CP002685 Genomic DNA. Translation: AEC07095.1.
CP002685 Genomic DNA. Translation: AEC07096.1.
AF360300 mRNA. Translation: AAK26010.1.
BT000983 mRNA. Translation: AAN41383.1.
AK316877 mRNA. Translation: BAH19585.1.
AK317470 mRNA. Translation: BAH20135.1.
AY085589 mRNA. Translation: AAM62810.1.
PIRiG84594.
RefSeqiNP_001031381.1. NM_001036304.1. [Q9C5E5-1]
NP_565492.1. NM_127660.4. [Q9C5E5-2]
NP_850007.1. NM_179676.3. [Q9C5E5-1]
NP_973498.1. NM_201769.3. [Q9C5E5-2]
UniGeneiAt.46381.
At.48498.

Genome annotation databases

EnsemblPlantsiAT2G20900.1; AT2G20900.1; AT2G20900. [Q9C5E5-1]
AT2G20900.2; AT2G20900.2; AT2G20900. [Q9C5E5-2]
AT2G20900.3; AT2G20900.3; AT2G20900. [Q9C5E5-2]
AT2G20900.4; AT2G20900.4; AT2G20900. [Q9C5E5-1]
GeneIDi816624.
GrameneiAT2G20900.1; AT2G20900.1; AT2G20900.
AT2G20900.2; AT2G20900.2; AT2G20900.
AT2G20900.3; AT2G20900.3; AT2G20900.
AT2G20900.4; AT2G20900.4; AT2G20900.
KEGGiath:AT2G20900.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDGK5_ARATH
AccessioniPrimary (citable) accession number: Q9C5E5
Secondary accession number(s): Q8LE70, Q9SKS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 1, 2001
Last modified: August 30, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families