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Q9C5E5

- DGK5_ARATH

UniProt

Q9C5E5 - DGK5_ARATH

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Protein

Diacylglycerol kinase 5

Gene
DGK5, At2g20900, F5H14.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress By similarity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: UniProtKB-EC
  3. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G20900-MONOMER.
ARA:GQT-2023-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 5 (EC:2.7.1.107)
Short name:
AtDGK5
Short name:
DAG kinase 5
Alternative name(s):
Diglyceride kinase 5
Short name:
DGK 5
Gene namesi
Name:DGK5
Ordered Locus Names:At2g20900
ORF Names:F5H14.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G20900.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Diacylglycerol kinase 5PRO_0000422113Add
BLAST

Proteomic databases

PaxDbiQ8LE70.
PRIDEiQ9C5E5.

Expressioni

Gene expression databases

ArrayExpressiQ9C5E5.
GenevestigatoriQ9C5E5.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

IntActiQ9C5E5. 1 interaction.
STRINGi3702.AT2G20900.4-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5E5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 187152DAGKcAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi24 – 329Poly-Glu

Sequence similaritiesi

Contains 1 DAGKc domain.

Phylogenomic databases

HOGENOMiHOG000241061.
InParanoidiQ9C5E5.
KOiK00901.
OMAiATHDCRS.
PhylomeDBiQ9C5E5.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
[Graphical view]
PfamiPF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTiSM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9C5E5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEKYNSLSDF LKEFYIPTYV LSAETEEEEE EESRPTPASP VLVFINSKSG    50
GQLGGELILT YRSLLNHNQV FDLDQETPDK VLRRIYLNLE RLKDDDFARQ 100
IREKLKIIVA GGDGTAGWLL GVVCDLKLSH PPPIATVPLG TGNNLPFAFG 150
WGKKNPGTDR TAVESFLEQV LKAKVMKIDN WHILMRMKTP KEGGSCDPVA 200
PLELPHSLHA FHRVSPTDEL NKEGCHTFRG GFWNYFSLGM DAQISYAFHS 250
ERKLHPEKFK NQLVNQSTYV KLGCTQGWFC ASLFHPASRN IAQLAKVKIA 300
TRNGQWQDLH IPHSIRSIVC LNLPSFSGGL NPWGTPNPRK QRDRGLTPPF 350
VDDGLIEVVG FRNAWHGLVL LAPNGHGTRL AQANRIRFEF HKGATDHTFM 400
RMDGEPWKQP LPLDDETVMV EISHLGQVNM LATHDCRSRS VFDPSTPRHQ 450
DGAEDYDDNE DDSVAEGEEF RKFGAADTFK IPDEGEHSNK KGRASRRRNS 500
NVHGWSHVL 509
Length:509
Mass (Da):57,377
Last modified:June 1, 2001 - v1
Checksum:iF6F9A08630D327DA
GO
Isoform 2 (identifier: Q9C5E5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     485-491: GEHSNKK → VDISQLS
     492-509: Missing.

Note: No experimental confirmation available.

Show »
Length:491
Mass (Da):55,267
Checksum:i117384E426570568
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei485 – 4917GEHSNKK → VDISQLS in isoform 2. VSP_046410
Alternative sequencei492 – 50918Missing in isoform 2. VSP_046411Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311E → Q in AAM62810. 1 Publication
Sequence conflicti97 – 971F → S in AAM62810. 1 Publication
Sequence conflicti102 – 1021R → G in AAM62810. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006234 Genomic DNA. Translation: AAD20931.2.
CP002685 Genomic DNA. Translation: AEC07093.1.
CP002685 Genomic DNA. Translation: AEC07094.1.
CP002685 Genomic DNA. Translation: AEC07095.1.
CP002685 Genomic DNA. Translation: AEC07096.1.
AF360300 mRNA. Translation: AAK26010.1.
BT000983 mRNA. Translation: AAN41383.1.
AK316877 mRNA. Translation: BAH19585.1.
AK317470 mRNA. Translation: BAH20135.1.
AY085589 mRNA. Translation: AAM62810.1.
PIRiG84594.
RefSeqiNP_001031381.1. NM_001036304.1. [Q9C5E5-1]
NP_565492.1. NM_127660.3. [Q9C5E5-2]
NP_850007.1. NM_179676.2. [Q9C5E5-1]
NP_973498.1. NM_201769.2. [Q9C5E5-2]
UniGeneiAt.46381.
At.48498.

Genome annotation databases

EnsemblPlantsiAT2G20900.1; AT2G20900.1; AT2G20900. [Q9C5E5-1]
AT2G20900.4; AT2G20900.4; AT2G20900. [Q9C5E5-1]
GeneIDi816624.
KEGGiath:AT2G20900.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006234 Genomic DNA. Translation: AAD20931.2 .
CP002685 Genomic DNA. Translation: AEC07093.1 .
CP002685 Genomic DNA. Translation: AEC07094.1 .
CP002685 Genomic DNA. Translation: AEC07095.1 .
CP002685 Genomic DNA. Translation: AEC07096.1 .
AF360300 mRNA. Translation: AAK26010.1 .
BT000983 mRNA. Translation: AAN41383.1 .
AK316877 mRNA. Translation: BAH19585.1 .
AK317470 mRNA. Translation: BAH20135.1 .
AY085589 mRNA. Translation: AAM62810.1 .
PIRi G84594.
RefSeqi NP_001031381.1. NM_001036304.1. [Q9C5E5-1 ]
NP_565492.1. NM_127660.3. [Q9C5E5-2 ]
NP_850007.1. NM_179676.2. [Q9C5E5-1 ]
NP_973498.1. NM_201769.2. [Q9C5E5-2 ]
UniGenei At.46381.
At.48498.

3D structure databases

ProteinModelPortali Q9C5E5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9C5E5. 1 interaction.
STRINGi 3702.AT2G20900.4-P.

Proteomic databases

PaxDbi Q8LE70.
PRIDEi Q9C5E5.

Protocols and materials databases

DNASUi 816624.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G20900.1 ; AT2G20900.1 ; AT2G20900 . [Q9C5E5-1 ]
AT2G20900.4 ; AT2G20900.4 ; AT2G20900 . [Q9C5E5-1 ]
GeneIDi 816624.
KEGGi ath:AT2G20900.

Organism-specific databases

TAIRi AT2G20900.

Phylogenomic databases

HOGENOMi HOG000241061.
InParanoidi Q9C5E5.
KOi K00901.
OMAi ATHDCRS.
PhylomeDBi Q9C5E5.

Enzyme and pathway databases

BioCyci ARA:AT2G20900-MONOMER.
ARA:GQT-2023-MONOMER.

Gene expression databases

ArrayExpressi Q9C5E5.
Genevestigatori Q9C5E5.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
[Graphical view ]
Pfami PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view ]
PIRSFi PIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTi SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Entry informationi

Entry nameiDGK5_ARATH
AccessioniPrimary (citable) accession number: Q9C5E5
Secondary accession number(s): Q8LE70, Q9SKS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi